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The map label for this gene is rihA [H]

Identifier: 187934547

GI number: 187934547

Start: 3121758

End: 3122738

Strand: Reverse

Name: rihA [H]

Synonym: CLL_A3021

Alternate gene names: 187934547

Gene position: 3122738-3121758 (Counterclockwise)

Preceding gene: 187934435

Following gene: 187932410

Centisome position: 82.17

GC content: 29.97

Gene sequence:

>981_bases
ATGAATAAGAAAAAAATAATAATAGATTGTGATCCTGGAATTGATGATTCATTAGCAATCATGCTTGCATTAAAATCGGA
GGAATTAGAAGTAAAAGGTATAACTATTGTTTCTGGAAATGTACATGCTAAAAAAGGTGCAGAAAATGCTCTTAAAATAT
TAAAAGAACTTGGCAGGTTAGATATTCCTGTATATATAGGAGATGGTGAACCATTAGTAAGGGAGCTAATTACAGCAGAA
GACACTCATGGAGGCGATGGACTTGGAGAAACATATTTACCTAAAGTAGAAAAAGCTAATTATAAAGATGGAGCAGTAGA
TTTTATGCTAAATTCTTTAAGAGAAGAGGATGAATTATCAATAATAGCAATAGGACCATTAACTAATATTGCAAAAGCTT
TAGATAAAGATAAAGAAACAACAAGGAAAATGAAAGAATTAATTTTAATGGGGGGAGCTTTTAAGAGCTTTGGAAACTGT
TCTCAAGTTGCTGAATTTAATTTCTGGGTAGATCCACATGGAGCAGAAAAAGTTTTTAATGAACTTAATAGAAAAATTAC
CATGGTTGGATTAGATGTAACAAGAAAAATTGTATTAACACCTAACTATATAGAAATGTTAAAGCAATTTAAGAGTCCAT
TAGCAGATTTAATAGTTAAAATAACAAGATTTTATGTGGATTTTCATTGGGAGCAAGAAAGAACTTTGGGGTGTGTGATA
AATGATCCATTAGCAATAGCTTATTTTATAGATTCAAGTATTTGTAGTGGAAAAGAATATTATGTGGATATAGTTACAGA
GGGAAAGGCAATTGGCATGAGCTTAGTAGATGAAGGGGATTTTTATAGAAAAGAACCAAATTGTTTGGTATTAACAGAAG
TAGATGCAAAAGCATTCATGGAAATGTTTTTAACAAGATTATTTCCAGAGCATAAGAAAGATATTTTTAATATATTAAAT
AATTCAAAGTACGGCAATTAA

Upstream 100 bases:

>100_bases
AATAATATATTGGTGTAGAAATCAGATTAATTGAAGAATGAATAGAAAAAATAGAGAGTTGTGGCTTTATTCTAGATATA
GAAGATAAGGAGGATTTTTA

Downstream 100 bases:

>100_bases
GCAATAAAAGGGGGAATTAAAATATGAAAAAAATAAATTTAAATGTAATGATATTTATGTCGTTATGTATAGTTATAAAT
CTTGTGGGGGGATTTATAGC

Product: nucleoside hydrolase, IUNH family

Products: uracil; ribose; cytosine [C]

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MNKKKIIIDCDPGIDDSLAIMLALKSEELEVKGITIVSGNVHAKKGAENALKILKELGRLDIPVYIGDGEPLVRELITAE
DTHGGDGLGETYLPKVEKANYKDGAVDFMLNSLREEDELSIIAIGPLTNIAKALDKDKETTRKMKELILMGGAFKSFGNC
SQVAEFNFWVDPHGAEKVFNELNRKITMVGLDVTRKIVLTPNYIEMLKQFKSPLADLIVKITRFYVDFHWEQERTLGCVI
NDPLAIAYFIDSSICSGKEYYVDIVTEGKAIGMSLVDEGDFYRKEPNCLVLTEVDAKAFMEMFLTRLFPEHKKDIFNILN
NSKYGN

Sequences:

>Translated_326_residues
MNKKKIIIDCDPGIDDSLAIMLALKSEELEVKGITIVSGNVHAKKGAENALKILKELGRLDIPVYIGDGEPLVRELITAE
DTHGGDGLGETYLPKVEKANYKDGAVDFMLNSLREEDELSIIAIGPLTNIAKALDKDKETTRKMKELILMGGAFKSFGNC
SQVAEFNFWVDPHGAEKVFNELNRKITMVGLDVTRKIVLTPNYIEMLKQFKSPLADLIVKITRFYVDFHWEQERTLGCVI
NDPLAIAYFIDSSICSGKEYYVDIVTEGKAIGMSLVDEGDFYRKEPNCLVLTEVDAKAFMEMFLTRLFPEHKKDIFNILN
NSKYGN
>Mature_326_residues
MNKKKIIIDCDPGIDDSLAIMLALKSEELEVKGITIVSGNVHAKKGAENALKILKELGRLDIPVYIGDGEPLVRELITAE
DTHGGDGLGETYLPKVEKANYKDGAVDFMLNSLREEDELSIIAIGPLTNIAKALDKDKETTRKMKELILMGGAFKSFGNC
SQVAEFNFWVDPHGAEKVFNELNRKITMVGLDVTRKIVLTPNYIEMLKQFKSPLADLIVKITRFYVDFHWEQERTLGCVI
NDPLAIAYFIDSSICSGKEYYVDIVTEGKAIGMSLVDEGDFYRKEPNCLVLTEVDAKAFMEMFLTRLFPEHKKDIFNILN
NSKYGN

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786871, Length=305, Percent_Identity=37.0491803278689, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI1788486, Length=302, Percent_Identity=34.4370860927152, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1786213, Length=303, Percent_Identity=32.3432343234323, Blast_Score=125, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI32565474, Length=198, Percent_Identity=33.8383838383838, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17565698, Length=296, Percent_Identity=30.0675675675676, Blast_Score=98, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6320608, Length=276, Percent_Identity=31.1594202898551, Blast_Score=110, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24583915, Length=239, Percent_Identity=28.8702928870293, Blast_Score=98, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24641839, Length=289, Percent_Identity=27.3356401384083, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24641837, Length=204, Percent_Identity=32.3529411764706, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022975 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: NA

Molecular weight: Translated: 36586; Mature: 36586

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKKIIIDCDPGIDDSLAIMLALKSEELEVKGITIVSGNVHAKKGAENALKILKELGRL
CCCCEEEEECCCCCCCCEEEEEEEECCCEEEEEEEEEECCCCCCCCHHHHHHHHHHHCCC
DIPVYIGDGEPLVRELITAEDTHGGDGLGETYLPKVEKANYKDGAVDFMLNSLREEDELS
CCEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEE
IIAIGPLTNIAKALDKDKETTRKMKELILMGGAFKSFGNCSQVAEFNFWVDPHGAEKVFN
EEEECCHHHHHHHHCCCHHHHHHHHHHHHHCCCHHCCCCCHHHHEEEEEECCCCHHHHHH
ELNRKITMVGLDVTRKIVLTPNYIEMLKQFKSPLADLIVKITRFYVDFHWEQERTLGCVI
HHCCEEEEEEECEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEEEE
NDPLAIAYFIDSSICSGKEYYVDIVTEGKAIGMSLVDEGDFYRKEPNCLVLTEVDAKAFM
CCCEEEEEEECCHHCCCCEEEEEEEECCCEECEEEECCCCCCCCCCCEEEEEECCHHHHH
EMFLTRLFPEHKKDIFNILNNSKYGN
HHHHHHHCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNKKKIIIDCDPGIDDSLAIMLALKSEELEVKGITIVSGNVHAKKGAENALKILKELGRL
CCCCEEEEECCCCCCCCEEEEEEEECCCEEEEEEEEEECCCCCCCCHHHHHHHHHHHCCC
DIPVYIGDGEPLVRELITAEDTHGGDGLGETYLPKVEKANYKDGAVDFMLNSLREEDELS
CCEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEE
IIAIGPLTNIAKALDKDKETTRKMKELILMGGAFKSFGNCSQVAEFNFWVDPHGAEKVFN
EEEECCHHHHHHHHCCCHHHHHHHHHHHHHCCCHHCCCCCHHHHEEEEEECCCCHHHHHH
ELNRKITMVGLDVTRKIVLTPNYIEMLKQFKSPLADLIVKITRFYVDFHWEQERTLGCVI
HHCCEEEEEEECEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEEEE
NDPLAIAYFIDSSICSGKEYYVDIVTEGKAIGMSLVDEGDFYRKEPNCLVLTEVDAKAFM
CCCEEEEEEECCHHCCCCEEEEEEEECCCEECEEEECCCCCCCCCCCEEEEEECCHHHHH
EMFLTRLFPEHKKDIFNILNNSKYGN
HHHHHHHCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uridine; H2O; cytidine [C]

Specific reaction: uridine + H2O = uracil + ribose cytidine + H2O = cytosine + ribose [C]

General reaction: Pyrimidine-specific nucleoside hydrolase [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA