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The map label for this gene is epsF [H]

Identifier: 187933799

GI number: 187933799

Start: 2508621

End: 2509697

Strand: Reverse

Name: epsF [H]

Synonym: CLL_A2425

Alternate gene names: 187933799

Gene position: 2509697-2508621 (Counterclockwise)

Preceding gene: 187934424

Following gene: 187934321

Centisome position: 66.04

GC content: 22.66

Gene sequence:

>1077_bases
ATGAGTATCTTTAAGAAAATACATTGTTTTCTTAAGAAAAGGTACAAAAAAATAAACTACAATGAATTAAGTTTGATTTC
TGGAAGCTTAGCACAATTATATAAAGATGGAATAAAAATAAATGAAGCTTTAAATTTAATAAAAGAGGTTGTATTAACTA
AAGAGTACAAAAGAAGTATTAGTGATATTGTTAAAAACATAGAAAATGGGATGACATTATCTAATGCTTTCGATGAAAAA
AGAGAGTTATATCCTAAGTTTTTTATAGGAATAATCTCTATAGGAGAAAATTCAGGAAAGTTATATGAAGCTTTAAAGAC
ATTGAAAAATTATTATGGGAAAAGATCTTTTATAAACAAGTATATTATAAGTTCATTAACATATCCAATGATATTAATGA
TAAGTATATTAGCTCTAATGGTATTTTTAACTTTAGTAGTTATACCTAATTTTCGTGGCATTTATTTATCAATAGGAATA
AAACCACCTTTTAGTTGTGAAAAAATATATGAATTAAGCAATTATATAAAAGAAAATCCGATTTTTAGTTTTATATTTTT
TGTGTGTTGGGGAGTTTTTATACCATTTATAAGCATAAAGAGATTAATAAATAAAAAATATGTAAGTTTATTAAGTAATT
TCAAGATTTTTAACAAGTTTTTAGAGTATGTAATTGTATTCATATTATCCATTATAGTTAATAGTGGAATGAATATTTCA
TATGGACTTAGGTATTGTTCAGAGAGTATGCATTTTAATTATATAAGTGACAAACTTAATAAAATTAATGAAGATATATT
AAAAGGAAGTACATTAAGTGAAGCAATAACTGATATAAATATATTTTCTAAATATACAGTTGCAGTAGTAAGAGTGAGAG
AAGAAAGTGGCTCTATAGGAGAAGCTTTAAAAGAACTAAGTTTAATATTAGAAGAATCTTTAATTAAAACAGTTAATAAA
TATCTAAGTTTATTGCAACCATTTCTTATAATATTTATATCAATAGTTATTACAGTATTTTTTATGATTTTTATTCTTCC
TTTATTTGATAGCTTAAAAATAGGAGCAATTAAGTGA

Upstream 100 bases:

>100_bases
TTATCTAATATTGGTATGAAAGATGATTTAGAGGAGTTATTAAAAAGAGGTCAAATATCCTATGAAGATTATGTACGGTT
TATGAAAGGAGAAGGATTAA

Downstream 100 bases:

>100_bases
AGAAAAAAGGATTTACTTTAATAGAAGTCATAGCAAGTTTATCAATAATTCTAATAATATTTTCTTTAACTTTATCAGTA
AAAGATATATATTCTACCAT

Product: pilus biogenesis protein

Products: NA

Alternate protein names: Cholera toxin secretion protein epsF [H]

Number of amino acids: Translated: 358; Mature: 357

Protein sequence:

>358_residues
MSIFKKIHCFLKKRYKKINYNELSLISGSLAQLYKDGIKINEALNLIKEVVLTKEYKRSISDIVKNIENGMTLSNAFDEK
RELYPKFFIGIISIGENSGKLYEALKTLKNYYGKRSFINKYIISSLTYPMILMISILALMVFLTLVVIPNFRGIYLSIGI
KPPFSCEKIYELSNYIKENPIFSFIFFVCWGVFIPFISIKRLINKKYVSLLSNFKIFNKFLEYVIVFILSIIVNSGMNIS
YGLRYCSESMHFNYISDKLNKINEDILKGSTLSEAITDINIFSKYTVAVVRVREESGSIGEALKELSLILEESLIKTVNK
YLSLLQPFLIIFISIVITVFFMIFILPLFDSLKIGAIK

Sequences:

>Translated_358_residues
MSIFKKIHCFLKKRYKKINYNELSLISGSLAQLYKDGIKINEALNLIKEVVLTKEYKRSISDIVKNIENGMTLSNAFDEK
RELYPKFFIGIISIGENSGKLYEALKTLKNYYGKRSFINKYIISSLTYPMILMISILALMVFLTLVVIPNFRGIYLSIGI
KPPFSCEKIYELSNYIKENPIFSFIFFVCWGVFIPFISIKRLINKKYVSLLSNFKIFNKFLEYVIVFILSIIVNSGMNIS
YGLRYCSESMHFNYISDKLNKINEDILKGSTLSEAITDINIFSKYTVAVVRVREESGSIGEALKELSLILEESLIKTVNK
YLSLLQPFLIIFISIVITVFFMIFILPLFDSLKIGAIK
>Mature_357_residues
SIFKKIHCFLKKRYKKINYNELSLISGSLAQLYKDGIKINEALNLIKEVVLTKEYKRSISDIVKNIENGMTLSNAFDEKR
ELYPKFFIGIISIGENSGKLYEALKTLKNYYGKRSFINKYIISSLTYPMILMISILALMVFLTLVVIPNFRGIYLSIGIK
PPFSCEKIYELSNYIKENPIFSFIFFVCWGVFIPFISIKRLINKKYVSLLSNFKIFNKFLEYVIVFILSIIVNSGMNISY
GLRYCSESMHFNYISDKLNKINEDILKGSTLSEAITDINIFSKYTVAVVRVREESGSIGEALKELSLILEESLIKTVNKY
LSLLQPFLIIFISIVITVFFMIFILPLFDSLKIGAIK

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG1459

COG function: function code NU; Type II secretory pathway, component PulF

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP F family [H]

Homologues:

Organism=Escherichia coli, GI1786295, Length=288, Percent_Identity=25.6944444444444, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1789724, Length=347, Percent_Identity=22.1902017291066, Blast_Score=92, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003004
- InterPro:   IPR011850
- InterPro:   IPR018076
- InterPro:   IPR001992 [H]

Pfam domain/function: PF00482 GSPII_F [H]

EC number: NA

Molecular weight: Translated: 41271; Mature: 41140

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIFKKIHCFLKKRYKKINYNELSLISGSLAQLYKDGIKINEALNLIKEVVLTKEYKRSI
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
SDIVKNIENGMTLSNAFDEKRELYPKFFIGIISIGENSGKLYEALKTLKNYYGKRSFINK
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
YIISSLTYPMILMISILALMVFLTLVVIPNFRGIYLSIGIKPPFSCEKIYELSNYIKENP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCH
IFSFIFFVCWGVFIPFISIKRLINKKYVSLLSNFKIFNKFLEYVIVFILSIIVNSGMNIS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
YGLRYCSESMHFNYISDKLNKINEDILKGSTLSEAITDINIFSKYTVAVVRVREESGSIG
HHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEHHHCCCHH
EALKELSLILEESLIKTVNKYLSLLQPFLIIFISIVITVFFMIFILPLFDSLKIGAIK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SIFKKIHCFLKKRYKKINYNELSLISGSLAQLYKDGIKINEALNLIKEVVLTKEYKRSI
CHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
SDIVKNIENGMTLSNAFDEKRELYPKFFIGIISIGENSGKLYEALKTLKNYYGKRSFINK
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
YIISSLTYPMILMISILALMVFLTLVVIPNFRGIYLSIGIKPPFSCEKIYELSNYIKENP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCH
IFSFIFFVCWGVFIPFISIKRLINKKYVSLLSNFKIFNKFLEYVIVFILSIIVNSGMNIS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
YGLRYCSESMHFNYISDKLNKINEDILKGSTLSEAITDINIFSKYTVAVVRVREESGSIG
HHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEHHHCCCHH
EALKELSLILEESLIKTVNKYLSLLQPFLIIFISIVITVFFMIFILPLFDSLKIGAIK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]