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The map label for this gene is outE [H]

Identifier: 187934424

GI number: 187934424

Start: 2509684

End: 2510712

Strand: Reverse

Name: outE [H]

Synonym: CLL_A2426

Alternate gene names: 187934424

Gene position: 2510712-2509684 (Counterclockwise)

Preceding gene: 187935164

Following gene: 187933799

Centisome position: 66.07

GC content: 26.82

Gene sequence:

>1029_bases
GTGGATATAAGATATAGAATTAATGGCGGATTAATTCTAGTTCACAAAATGCTTTTATCTGAATATTTAACTTTAGCTTC
AAAAATAAAACTTAAAGCTAATATGGATATATCAGAAAGAAGAAAGCCACAAGATGGAAAAATAATTATAAATTACAACG
ATAATAATTATGATTTAAGGGTTTCATCTATTCCCGTAGTTTATGGTGAAAAAATAGTTATAAGAATACTATATAGCGAA
AATTTCAACTATAAATTAGACGATTTATACTACTCAAAAGAAAAAATAGAATTAATAAAAAAGATTATGTCATTAAAGAA
AGGTCTAGTTTTAGTTAATGGACCAACTGGAAGTGGGAAATCGACAACTCTGTATACTATATTGAAAGAAATCAATATAG
AAGATATAAATATAACAACTCTAGAAGACCCAGTGGAAGCAATCATTCCAAATATCAATCAAATGAGTTTAAATAAAAAA
TTAGATATAAATTTTTCAAATGGTTTAAAAAATATATTAAGGCAAGATCCAGACGTTATCATGATTGGTGAGGTTAGAGA
TGAAGAAACGGCAGCTATGTCTGTAAGAGCATCAATAACAGGACATAAAGTATATACAACTATCCATACGTCAACACCAA
GAGAAGTCTTTCTCAGACTTGAGGATATGGGAATAAAACCATATTTACTTAGGGATTCAATTGTAGGAATAATATCCCAA
AGATTAATAAAAATATTGTGTGAAAATTGTAAAAAAGAAGACATTAAGAATACTTATAAGGGAATTAAATTATATAAAAG
AGCTAGTTGTTCAAAATGTAATTATTCTGGTTATGAATCAAGAAAACTTATAAGTGCTGTATACTTTTTAGATAAGGAAT
ATGATGAAATTGATATTTATAAAGATAAGTACTGCTTATCTAATATTGGTATGAAAGATGATTTAGAGGAGTTATTAAAA
AGAGGTCAAATATCCTATGAAGATTATGTACGGTTTATGAAAGGAGAAGGATTAAATGAGTATCTTTAA

Upstream 100 bases:

>100_bases
TATATTTAAAGTAGAAGATGAAGATATAGAAAACACTTTAATTTATAATGCTATTTTAAGGAATGCTAGTGATATACATT
TTGAACCTTGCGAAAAAGAG

Downstream 100 bases:

>100_bases
GAAAATACATTGTTTTCTTAAGAAAAGGTACAAAAAAATAAACTACAATGAATTAAGTTTGATTTCTGGAAGCTTAGCAC
AATTATATAAAGATGGAATA

Product: type II/IV secretion system protein

Products: NA

Alternate protein names: Pectic enzymes secretion protein outE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MDIRYRINGGLILVHKMLLSEYLTLASKIKLKANMDISERRKPQDGKIIINYNDNNYDLRVSSIPVVYGEKIVIRILYSE
NFNYKLDDLYYSKEKIELIKKIMSLKKGLVLVNGPTGSGKSTTLYTILKEINIEDINITTLEDPVEAIIPNINQMSLNKK
LDINFSNGLKNILRQDPDVIMIGEVRDEETAAMSVRASITGHKVYTTIHTSTPREVFLRLEDMGIKPYLLRDSIVGIISQ
RLIKILCENCKKEDIKNTYKGIKLYKRASCSKCNYSGYESRKLISAVYFLDKEYDEIDIYKDKYCLSNIGMKDDLEELLK
RGQISYEDYVRFMKGEGLNEYL

Sequences:

>Translated_342_residues
MDIRYRINGGLILVHKMLLSEYLTLASKIKLKANMDISERRKPQDGKIIINYNDNNYDLRVSSIPVVYGEKIVIRILYSE
NFNYKLDDLYYSKEKIELIKKIMSLKKGLVLVNGPTGSGKSTTLYTILKEINIEDINITTLEDPVEAIIPNINQMSLNKK
LDINFSNGLKNILRQDPDVIMIGEVRDEETAAMSVRASITGHKVYTTIHTSTPREVFLRLEDMGIKPYLLRDSIVGIISQ
RLIKILCENCKKEDIKNTYKGIKLYKRASCSKCNYSGYESRKLISAVYFLDKEYDEIDIYKDKYCLSNIGMKDDLEELLK
RGQISYEDYVRFMKGEGLNEYL
>Mature_342_residues
MDIRYRINGGLILVHKMLLSEYLTLASKIKLKANMDISERRKPQDGKIIINYNDNNYDLRVSSIPVVYGEKIVIRILYSE
NFNYKLDDLYYSKEKIELIKKIMSLKKGLVLVNGPTGSGKSTTLYTILKEINIEDINITTLEDPVEAIIPNINQMSLNKK
LDINFSNGLKNILRQDPDVIMIGEVRDEETAAMSVRASITGHKVYTTIHTSTPREVFLRLEDMGIKPYLLRDSIVGIISQ
RLIKILCENCKKEDIKNTYKGIKLYKRASCSKCNYSGYESRKLISAVYFLDKEYDEIDIYKDKYCLSNIGMKDDLEELLK
RGQISYEDYVRFMKGEGLNEYL

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of pectate lyases and cellulases across the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=303, Percent_Identity=40.2640264026403, Blast_Score=245, Evalue=3e-66,
Organism=Escherichia coli, GI1786296, Length=346, Percent_Identity=31.7919075144509, Blast_Score=215, Evalue=3e-57,
Organism=Escherichia coli, GI87082188, Length=171, Percent_Identity=37.4269005847953, Blast_Score=102, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 39503; Mature: 39503

Theoretical pI: Translated: 8.74; Mature: 8.74

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIRYRINGGLILVHKMLLSEYLTLASKIKLKANMDISERRKPQDGKIIINYNDNNYDLR
CCEEEEECCCCHHHHHHHHHHHHHHHHHEEEEECCCHHHCCCCCCCEEEEEECCCCEEEE
VSSIPVVYGEKIVIRILYSENFNYKLDDLYYSKEKIELIKKIMSLKKGLVLVNGPTGSGK
EEEECEEECCEEEEEEEECCCCCEEHHHHEECHHHHHHHHHHHHHHCCEEEEECCCCCCC
STTLYTILKEINIEDINITTLEDPVEAIIPNINQMSLNKKLDINFSNGLKNILRQDPDVI
CHHHHHHHHHCCCCEEEEEECCCCHHHHCCCCCCEECCCEEEEEHHHHHHHHHHCCCCEE
MIGEVRDEETAAMSVRASITGHKVYTTIHTSTPREVFLRLEDMGIKPYLLRDSIVGIISQ
EEECCCCCCHHHEEEEEEECCCEEEEEEECCCCHHHEEEHCCCCCCCEEHHHHHHHHHHH
RLIKILCENCKKEDIKNTYKGIKLYKRASCSKCNYSGYESRKLISAVYFLDKEYDEIDIY
HHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEE
KDKYCLSNIGMKDDLEELLKRGQISYEDYVRFMKGEGLNEYL
CCHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MDIRYRINGGLILVHKMLLSEYLTLASKIKLKANMDISERRKPQDGKIIINYNDNNYDLR
CCEEEEECCCCHHHHHHHHHHHHHHHHHEEEEECCCHHHCCCCCCCEEEEEECCCCEEEE
VSSIPVVYGEKIVIRILYSENFNYKLDDLYYSKEKIELIKKIMSLKKGLVLVNGPTGSGK
EEEECEEECCEEEEEEEECCCCCEEHHHHEECHHHHHHHHHHHHHHCCEEEEECCCCCCC
STTLYTILKEINIEDINITTLEDPVEAIIPNINQMSLNKKLDINFSNGLKNILRQDPDVI
CHHHHHHHHHCCCCEEEEEECCCCHHHHCCCCCCEECCCEEEEEHHHHHHHHHHCCCCEE
MIGEVRDEETAAMSVRASITGHKVYTTIHTSTPREVFLRLEDMGIKPYLLRDSIVGIISQ
EEECCCCCCHHHEEEEEEECCCEEEEEEECCCCHHHEEEHCCCCCCCEEHHHHHHHHHHH
RLIKILCENCKKEDIKNTYKGIKLYKRASCSKCNYSGYESRKLISAVYFLDKEYDEIDIY
HHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEE
KDKYCLSNIGMKDDLEELLKRGQISYEDYVRFMKGEGLNEYL
CCHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1429461 [H]