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The map label for this gene is rlmI [H]

Identifier: 187933779

GI number: 187933779

Start: 92015

End: 93205

Strand: Reverse

Name: rlmI [H]

Synonym: CLL_A0085

Alternate gene names: 187933779

Gene position: 93205-92015 (Counterclockwise)

Preceding gene: 187932813

Following gene: 187933911

Centisome position: 2.45

GC content: 29.3

Gene sequence:

>1191_bases
ATGGCAAGTAAATTTTATTTACATAAGGGACGTAGCGGTAAGGCTGAACAAGGTCGTCCTTGGGTTTATACTGATGAAAT
AAATGAATATGATGGTGAATATGAAAATGGTGATATCGTAGAGGTATACAATCATAAGGGTTACTTTATCGGTAAAGGTT
ACATAAATGATAAAAGTAAAATAACCATCAGAATAATGTCAAGAGATATAAATGAAAATATAGATGAGGAATTCTTCAAA
AGCAGATTTAAAACAGCTTTTGAATATAGAAGAAGTATCATTGATGATTCATCTTGTAGAATGATATTTGGAGAAGCTGA
TTTTCTTCCTGGATTAACAGTTGATAAATTTGAAGATTATTATGTAATTCAAATTTCTACACTTGGAATGGATCAATATA
AAGATTTAATTGTTAAAGTATTAGTTAATGAATATAATGCTAAAGGTGTCTATGAAAGAAGTGACATAGCAACTAGAGAA
ATAGAAGGTTTAGAACAAACTAGGGGATTTTTAACTGAACCTTTTGATACTAATGTTGAAATTGTTGAAAATGGAGTTAA
GTACATAGTTGATCTAGCAAATGGTCAAAAAACTGGATTCTTCTTAGATCAAAAAGAAAATAGAGCTGCTATTCATAGAA
TATGTAAAGATAAAGACGTATTAGATTGCTTTACTCATACTGGTTCTTTTGCTTTAAATGCTGGTATTGCTGGTGCAAAA
TCAGTTCTTGGAATAGATGTTTCTCAGCATGCCATAGATTGTGCTAATAAAAATGCTGAATTAAACAATCTTCAAGATAC
AGTTAAATTTGAATGTCATAATGCTTTCGATGTTCTTCCTGCATGGTCAAGAGAAGAAAAGAAATTTGACGTTGTAATAC
TTGATCCTCCTGCATTTACAAAGTCTAGAAATACTATTAATGCAGCAAAAAGAGGATATAAAGAAATTAACTTAAGAGGT
TTAAAATTAGTTAAACATGGTGGTTACTTCATAACTTGCTCTTGTTCTCATTACATGAATGAAGAACTTTTAAAGAAAAC
TATTTTAGAAGCTTGCCAAGATGCTAATAAACTTATAAGACAAGTAGAATTTAGAACTCAATCTTGTGATCATCCAATAC
TTTGGAATTCAGATGAAAGTTACTATTTAAAATTCTTCATATTCCAAGTATTTGATAAAACAAAATTATAA

Upstream 100 bases:

>100_bases
TATTATTACAATAGGCTCATTAAAAAAGACTTTTCTTAATAATCTAAGAATAAGGCCTATTATTAAATATAATAATTATT
TTGGAAGGGGTCTTTTTAAT

Downstream 100 bases:

>100_bases
AAAAGTGGCTTATGCCACTTTTTTTATAGTTTAATTATCTTATCTCCATCATAAATCGGAACCACATCTATTATACTTTT
CTTTATACAATATTCTATAT

Product: PUA domain containing protein

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MASKFYLHKGRSGKAEQGRPWVYTDEINEYDGEYENGDIVEVYNHKGYFIGKGYINDKSKITIRIMSRDINENIDEEFFK
SRFKTAFEYRRSIIDDSSCRMIFGEADFLPGLTVDKFEDYYVIQISTLGMDQYKDLIVKVLVNEYNAKGVYERSDIATRE
IEGLEQTRGFLTEPFDTNVEIVENGVKYIVDLANGQKTGFFLDQKENRAAIHRICKDKDVLDCFTHTGSFALNAGIAGAK
SVLGIDVSQHAIDCANKNAELNNLQDTVKFECHNAFDVLPAWSREEKKFDVVILDPPAFTKSRNTINAAKRGYKEINLRG
LKLVKHGGYFITCSCSHYMNEELLKKTILEACQDANKLIRQVEFRTQSCDHPILWNSDESYYLKFFIFQVFDKTKL

Sequences:

>Translated_396_residues
MASKFYLHKGRSGKAEQGRPWVYTDEINEYDGEYENGDIVEVYNHKGYFIGKGYINDKSKITIRIMSRDINENIDEEFFK
SRFKTAFEYRRSIIDDSSCRMIFGEADFLPGLTVDKFEDYYVIQISTLGMDQYKDLIVKVLVNEYNAKGVYERSDIATRE
IEGLEQTRGFLTEPFDTNVEIVENGVKYIVDLANGQKTGFFLDQKENRAAIHRICKDKDVLDCFTHTGSFALNAGIAGAK
SVLGIDVSQHAIDCANKNAELNNLQDTVKFECHNAFDVLPAWSREEKKFDVVILDPPAFTKSRNTINAAKRGYKEINLRG
LKLVKHGGYFITCSCSHYMNEELLKKTILEACQDANKLIRQVEFRTQSCDHPILWNSDESYYLKFFIFQVFDKTKL
>Mature_395_residues
ASKFYLHKGRSGKAEQGRPWVYTDEINEYDGEYENGDIVEVYNHKGYFIGKGYINDKSKITIRIMSRDINENIDEEFFKS
RFKTAFEYRRSIIDDSSCRMIFGEADFLPGLTVDKFEDYYVIQISTLGMDQYKDLIVKVLVNEYNAKGVYERSDIATREI
EGLEQTRGFLTEPFDTNVEIVENGVKYIVDLANGQKTGFFLDQKENRAAIHRICKDKDVLDCFTHTGSFALNAGIAGAKS
VLGIDVSQHAIDCANKNAELNNLQDTVKFECHNAFDVLPAWSREEKKFDVVILDPPAFTKSRNTINAAKRGYKEINLRGL
KLVKHGGYFITCSCSHYMNEELLKKTILEACQDANKLIRQVEFRTQSCDHPILWNSDESYYLKFFIFQVFDKTKL

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=396, Percent_Identity=34.5959595959596, Blast_Score=274, Evalue=8e-75,
Organism=Escherichia coli, GI1787181, Length=264, Percent_Identity=25.3787878787879, Blast_Score=79, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 45592; Mature: 45461

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASKFYLHKGRSGKAEQGRPWVYTDEINEYDGEYENGDIVEVYNHKGYFIGKGYINDKSK
CCCCEEEECCCCCCCCCCCCEEEECCCCCCCCCCCCCCEEEEECCCCEEEECCEECCCCE
ITIRIMSRDINENIDEEFFKSRFKTAFEYRRSIIDDSSCRMIFGEADFLPGLTVDKFEDY
EEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCE
YVIQISTLGMDQYKDLIVKVLVNEYNAKGVYERSDIATREIEGLEQTRGFLTEPFDTNVE
EEEEEEECCCHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHCCCCCCCCCCCHH
IVENGVKYIVDLANGQKTGFFLDQKENRAAIHRICKDKDVLDCFTHTGSFALNAGIAGAK
HHHCCCEEEEECCCCCCCCEEEECCCCHHHHHHHHCCCCHHHHHHCCCCEEEECCCCCCC
SVLGIDVSQHAIDCANKNAELNNLQDTVKFECHNAFDVLPAWSREEKKFDVVILDPPAFT
CEEECCHHHHHHHHCCCCCCCCCCCHHEEEEECCCEECCCCCCCCCCCEEEEEECCCCCC
KSRNTINAAKRGYKEINLRGLKLVKHGGYFITCSCSHYMNEELLKKTILEACQDANKLIR
CCCCHHHHHHCCCHHCCCEEEEEEECCCEEEEEECHHHCCHHHHHHHHHHHHHHHHHHHH
QVEFRTQSCDHPILWNSDESYYLKFFIFQVFDKTKL
HHHHHHCCCCCCEEECCCCCEEHHHHHHHHHHHCCC
>Mature Secondary Structure 
ASKFYLHKGRSGKAEQGRPWVYTDEINEYDGEYENGDIVEVYNHKGYFIGKGYINDKSK
CCCEEEECCCCCCCCCCCCEEEECCCCCCCCCCCCCCEEEEECCCCEEEECCEECCCCE
ITIRIMSRDINENIDEEFFKSRFKTAFEYRRSIIDDSSCRMIFGEADFLPGLTVDKFEDY
EEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCE
YVIQISTLGMDQYKDLIVKVLVNEYNAKGVYERSDIATREIEGLEQTRGFLTEPFDTNVE
EEEEEEECCCHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHCCCCCCCCCCCHH
IVENGVKYIVDLANGQKTGFFLDQKENRAAIHRICKDKDVLDCFTHTGSFALNAGIAGAK
HHHCCCEEEEECCCCCCCCEEEECCCCHHHHHHHHCCCCHHHHHHCCCCEEEECCCCCCC
SVLGIDVSQHAIDCANKNAELNNLQDTVKFECHNAFDVLPAWSREEKKFDVVILDPPAFT
CEEECCHHHHHHHHCCCCCCCCCCCHHEEEEECCCEECCCCCCCCCCCEEEEEECCCCCC
KSRNTINAAKRGYKEINLRGLKLVKHGGYFITCSCSHYMNEELLKKTILEACQDANKLIR
CCCCHHHHHHCCCHHCCCEEEEEEECCCEEEEEECHHHCCHHHHHHHHHHHHHHHHHHHH
QVEFRTQSCDHPILWNSDESYYLKFFIFQVFDKTKL
HHHHHHCCCCCCEEECCCCCEEHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA