| Definition | Clostridium botulinum B str. Eklund 17B, complete genome. |
|---|---|
| Accession | NC_010674 |
| Length | 3,800,327 |
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The map label for this gene is epsC [H]
Identifier: 187933244
GI number: 187933244
Start: 3308638
End: 3310500
Strand: Reverse
Name: epsC [H]
Synonym: CLL_A3186
Alternate gene names: 187933244
Gene position: 3310500-3308638 (Counterclockwise)
Preceding gene: 187932491
Following gene: 187935706
Centisome position: 87.11
GC content: 29.58
Gene sequence:
>1863_bases ATGAGAAATTGGAAGACATTAATAATTATGATAATCGATATATTTATGGTAAATATGGCATACCTTTTTGCTATAAATAT AACTTTAAGTGGAAGGTTCACAGAAATTGCAAAAATATATACAGAAGATGTTATAGCTGTAAGTTTAATATATTTAGTGT GTTTTTACTTATTTAAAATGTATGAAAGCTTGTGGCATTTAACAGGAACAGATGAATTTCTACTAGGTGTTGGAGGATCG ATTTTAGCAGGAATATTATCAATTGGATATACTAGATTTTGGGGATCTGTTATTCCATTAAATGTTTCAGTTGTAGGCAT TCTTTTAAGCATTTTCTTTGTATTAGGTTATAGAATTTTATATAGAGTATATAGAAGAACTCTTTTGTACATACCATTTA AATATTCATCAGATCAAAGAAGAGTAATGATTGTTGGTGCTGGTTCAGCAGGAACTATGATCATTAATGAAATGATGGCA AGAAGAGAATTAAAATATAATCCCATAGTTCTTATTGATGATGATAAAGAAAAGCTTGGAAGAAGAATTTCAGGTGTAAA GATAGTAGGAAATAGATACGATATACCATATGTTGTAGGAGAACAAGAAATTGATTTAATATTAATATCAATACCTTCTC TTGATTCAAAAAATAAGGCAGAAATTATTGATATTTGTAAAAAGACTAATTGCAAGCTTCAAATAATTCCAGGTATATAT GAAATATTAAGTGGAGATGCAAATGTTAGTAGAATTAAAGATGTAGATTTAGAAGATTTACTAGGAAGAGATCCTATTGT ATTGGATAACAAAGGTATCTCAGATTATATACAAGGAAAAACAATATTAGTTACCGGAGCAGGTGGATCAATAGGTTCTG AGCTTTGTAGACAAATATCAGTGTATAATCCTAAAAGACTTATATTATTTGATATATATGAAAACAATATATACGATATT CAAAATGAATTAAAAGAAGAGTTCCCGGATATGAACCTTACTGTTTTAATTGGTTCTATAAGAGATAGGCAAAGATTACA TGAGGTATTTAGTAAATATAAAATAAATGTTGTATTCCATGCAGCAGCACATAAACATGTACCATTAATGGAGGATAGTC CTAAAGAAGCTGTTAAGAATAATGTTTTTGGAACATTAAATTTAGCAACTGAAGCAAGTAAAGCTAAGATTGATAGATTT GTAATGATTTCTACTGATAAAGCTGTTAATCCAACAAATATAATGGGAGCAACTAAAAGATTATGTGAAATGATTGTTCA AGCTATGGATAAACAGTCAAAAACTGAGTTTGTTGCTGTTAGATTTGGAAATGTTCTTGGTAGTAATGGATCAGTAATTC CATTATTTAAAAAGCAAATAGCTAATGGTGGTCCAGTTACGCTTACTCATAAAAAGATAGTTAGATACTTTATGCTGATT CCAGAAGCCGCCCAATTAGTGCTTCAAGCAGGTGCATTTGCTAAGGGTGGAGAAGTATTTGTACTAGATATGGGAAAACC AGTTAAGATTTATGACTTAGCTTGTGATCTTATAAGACTTTCAGGATTTGAACCTAATAGAGATATCAAAATAGTTGTTA CTGGACTTAGACCAGGAGAAAAATTATATGAAGAACTTTTAATGAGTGAAGAAGGTTTAAAAGACACTGCCCATCAAAAG ATATACGTTGGAAAACCAACTTTTGAAGATATGGACACATTAAATTATAAGCTTGAACAATTACGTAAGTTATTAGAGTT AAATGATATAAATGAAATTAAACATCAAATGCAAACTATTGTTCCAACATATCATTACAAAAACGAAGATGAAGTTGCAG CAGAAAATGCTGATAAGGAGTAA
Upstream 100 bases:
>100_bases AAGTTACTAATAAATGCGTTAAATAAAATGAGGTAATTAAAATAGAGTCTTACTTGCTATAAAGTTTAGCAGGAATCTCT CCAGGTAGGAGGAAACTATA
Downstream 100 bases:
>100_bases GTATGAGTAGAGAAGATAAGACTAGAATCACATTTATAACACTGATATCGATAATTGCTATGGTTTTTGGAGTAGCTAAT GGAAATTATGTAATACCTAT
Product: capsular polysaccharide biosynthesis protein
Products: UDPglucoseal [C]
Alternate protein names: NA
Number of amino acids: Translated: 620; Mature: 620
Protein sequence:
>620_residues MRNWKTLIIMIIDIFMVNMAYLFAINITLSGRFTEIAKIYTEDVIAVSLIYLVCFYLFKMYESLWHLTGTDEFLLGVGGS ILAGILSIGYTRFWGSVIPLNVSVVGILLSIFFVLGYRILYRVYRRTLLYIPFKYSSDQRRVMIVGAGSAGTMIINEMMA RRELKYNPIVLIDDDKEKLGRRISGVKIVGNRYDIPYVVGEQEIDLILISIPSLDSKNKAEIIDICKKTNCKLQIIPGIY EILSGDANVSRIKDVDLEDLLGRDPIVLDNKGISDYIQGKTILVTGAGGSIGSELCRQISVYNPKRLILFDIYENNIYDI QNELKEEFPDMNLTVLIGSIRDRQRLHEVFSKYKINVVFHAAAHKHVPLMEDSPKEAVKNNVFGTLNLATEASKAKIDRF VMISTDKAVNPTNIMGATKRLCEMIVQAMDKQSKTEFVAVRFGNVLGSNGSVIPLFKKQIANGGPVTLTHKKIVRYFMLI PEAAQLVLQAGAFAKGGEVFVLDMGKPVKIYDLACDLIRLSGFEPNRDIKIVVTGLRPGEKLYEELLMSEEGLKDTAHQK IYVGKPTFEDMDTLNYKLEQLRKLLELNDINEIKHQMQTIVPTYHYKNEDEVAAENADKE
Sequences:
>Translated_620_residues MRNWKTLIIMIIDIFMVNMAYLFAINITLSGRFTEIAKIYTEDVIAVSLIYLVCFYLFKMYESLWHLTGTDEFLLGVGGS ILAGILSIGYTRFWGSVIPLNVSVVGILLSIFFVLGYRILYRVYRRTLLYIPFKYSSDQRRVMIVGAGSAGTMIINEMMA RRELKYNPIVLIDDDKEKLGRRISGVKIVGNRYDIPYVVGEQEIDLILISIPSLDSKNKAEIIDICKKTNCKLQIIPGIY EILSGDANVSRIKDVDLEDLLGRDPIVLDNKGISDYIQGKTILVTGAGGSIGSELCRQISVYNPKRLILFDIYENNIYDI QNELKEEFPDMNLTVLIGSIRDRQRLHEVFSKYKINVVFHAAAHKHVPLMEDSPKEAVKNNVFGTLNLATEASKAKIDRF VMISTDKAVNPTNIMGATKRLCEMIVQAMDKQSKTEFVAVRFGNVLGSNGSVIPLFKKQIANGGPVTLTHKKIVRYFMLI PEAAQLVLQAGAFAKGGEVFVLDMGKPVKIYDLACDLIRLSGFEPNRDIKIVVTGLRPGEKLYEELLMSEEGLKDTAHQK IYVGKPTFEDMDTLNYKLEQLRKLLELNDINEIKHQMQTIVPTYHYKNEDEVAAENADKE >Mature_620_residues MRNWKTLIIMIIDIFMVNMAYLFAINITLSGRFTEIAKIYTEDVIAVSLIYLVCFYLFKMYESLWHLTGTDEFLLGVGGS ILAGILSIGYTRFWGSVIPLNVSVVGILLSIFFVLGYRILYRVYRRTLLYIPFKYSSDQRRVMIVGAGSAGTMIINEMMA RRELKYNPIVLIDDDKEKLGRRISGVKIVGNRYDIPYVVGEQEIDLILISIPSLDSKNKAEIIDICKKTNCKLQIIPGIY EILSGDANVSRIKDVDLEDLLGRDPIVLDNKGISDYIQGKTILVTGAGGSIGSELCRQISVYNPKRLILFDIYENNIYDI QNELKEEFPDMNLTVLIGSIRDRQRLHEVFSKYKINVVFHAAAHKHVPLMEDSPKEAVKNNVFGTLNLATEASKAKIDRF VMISTDKAVNPTNIMGATKRLCEMIVQAMDKQSKTEFVAVRFGNVLGSNGSVIPLFKKQIANGGPVTLTHKKIVRYFMLI PEAAQLVLQAGAFAKGGEVFVLDMGKPVKIYDLACDLIRLSGFEPNRDIKIVVTGLRPGEKLYEELLMSEEGLKDTAHQK IYVGKPTFEDMDTLNYKLEQLRKLLELNDINEIKHQMQTIVPTYHYKNEDEVAAENADKE
Specific function: Involved in biofilm formation [H]
COG id: COG1086
COG function: function code MG; Predicted nucleoside-diphosphate sugar epimerases
Gene ontology:
Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the polysaccharide synthase family [H]
Homologues:
Organism=Homo sapiens, GI7657641, Length=266, Percent_Identity=25.5639097744361, Blast_Score=71, Evalue=3e-12,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR016040 - InterPro: IPR003869 [H]
Pfam domain/function: PF02719 Polysacc_synt_2 [H]
EC number: 5.1.3.2 [C]
Molecular weight: Translated: 70050; Mature: 70050
Theoretical pI: Translated: 7.54; Mature: 7.54
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.0 %Cys (Translated Protein) 3.2 %Met (Translated Protein) 4.2 %Cys+Met (Translated Protein) 1.0 %Cys (Mature Protein) 3.2 %Met (Mature Protein) 4.2 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MRNWKTLIIMIIDIFMVNMAYLFAINITLSGRFTEIAKIYTEDVIAVSLIYLVCFYLFKM CCCHHHHHHHHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH YESLWHLTGTDEFLLGVGGSILAGILSIGYTRFWGSVIPLNVSVVGILLSIFFVLGYRIL HHHHHCCCCCCHHHECCCHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHHH YRVYRRTLLYIPFKYSSDQRRVMIVGAGSAGTMIINEMMARRELKYNPIVLIDDDKEKLG HHHHHHHEEEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEECCHHHHC RRISGVKIVGNRYDIPYVVGEQEIDLILISIPSLDSKNKAEIIDICKKTNCKLQIIPGIY CHHCCEEEECCCCCCCEEECCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCEEEECCCHH EILSGDANVSRIKDVDLEDLLGRDPIVLDNKGISDYIQGKTILVTGAGGSIGSELCRQIS HHHCCCCCHHHCCCCCHHHHCCCCCEEECCCCCHHHHCCCEEEEECCCCCHHHHHHHHHH VYNPKRLILFDIYENNIYDIQNELKEEFPDMNLTVLIGSIRDRQRLHEVFSKYKINVVFH CCCCCEEEEEEEECCCHHHHHHHHHHHCCCCEEEEEEECHHHHHHHHHHHHHEEEEEEEE AAAHKHVPLMEDSPKEAVKNNVFGTLNLATEASKAKIDRFVMISTDKAVNPTNIMGATKR ECCCCCCCCCCCCHHHHHHCCCCEEEEECCCCHHHCCCEEEEEECCCCCCCHHHHHHHHH LCEMIVQAMDKQSKTEFVAVRFGNVLGSNGSVIPLFKKQIANGGPVTLTHKKIVRYFMLI HHHHHHHHHCCCCCCCEEEEEECHHCCCCCCEEHHHHHHHCCCCCEEEEHHHHHHHHHHC PEAAQLVLQAGAFAKGGEVFVLDMGKPVKIYDLACDLIRLSGFEPNRDIKIVVTGLRPGE CHHHHHHHHHCCCCCCCEEEEEECCCCEEEHHHHHHHHHHCCCCCCCCEEEEEECCCCCH KLYEELLMSEEGLKDTAHQKIYVGKPTFEDMDTLNYKLEQLRKLLELNDINEIKHQMQTI HHHHHHHHCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH VPTYHYKNEDEVAAENADKE CCCEECCCCCHHHCCCCCCC >Mature Secondary Structure MRNWKTLIIMIIDIFMVNMAYLFAINITLSGRFTEIAKIYTEDVIAVSLIYLVCFYLFKM CCCHHHHHHHHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH YESLWHLTGTDEFLLGVGGSILAGILSIGYTRFWGSVIPLNVSVVGILLSIFFVLGYRIL HHHHHCCCCCCHHHECCCHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHHH YRVYRRTLLYIPFKYSSDQRRVMIVGAGSAGTMIINEMMARRELKYNPIVLIDDDKEKLG HHHHHHHEEEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEECCHHHHC RRISGVKIVGNRYDIPYVVGEQEIDLILISIPSLDSKNKAEIIDICKKTNCKLQIIPGIY CHHCCEEEECCCCCCCEEECCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCEEEECCCHH EILSGDANVSRIKDVDLEDLLGRDPIVLDNKGISDYIQGKTILVTGAGGSIGSELCRQIS HHHCCCCCHHHCCCCCHHHHCCCCCEEECCCCCHHHHCCCEEEEECCCCCHHHHHHHHHH VYNPKRLILFDIYENNIYDIQNELKEEFPDMNLTVLIGSIRDRQRLHEVFSKYKINVVFH CCCCCEEEEEEEECCCHHHHHHHHHHHCCCCEEEEEEECHHHHHHHHHHHHHEEEEEEEE AAAHKHVPLMEDSPKEAVKNNVFGTLNLATEASKAKIDRFVMISTDKAVNPTNIMGATKR ECCCCCCCCCCCCHHHHHHCCCCEEEEECCCCHHHCCCEEEEEECCCCCCCHHHHHHHHH LCEMIVQAMDKQSKTEFVAVRFGNVLGSNGSVIPLFKKQIANGGPVTLTHKKIVRYFMLI HHHHHHHHHCCCCCCCEEEEEECHHCCCCCCEEHHHHHHHCCCCCEEEEHHHHHHHHHHC PEAAQLVLQAGAFAKGGEVFVLDMGKPVKIYDLACDLIRLSGFEPNRDIKIVVTGLRPGE CHHHHHHHHHCCCCCCCEEEEEECCCCEEEHHHHHHHHHHCCCCCCCCEEEEEECCCCCH KLYEELLMSEEGLKDTAHQKIYVGKPTFEDMDTLNYKLEQLRKLLELNDINEIKHQMQTI HHHHHHHHCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH VPTYHYKNEDEVAAENADKE CCCEECCCCCHHHCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NAD+ [C]
Metal ions: NA
Kcat value (1/min): 57600 [C]
Specific activity: 233.3
Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]
Substrates: UDPglucose [C]
Specific reaction: UDPglucose <==> UDPglucoseal [C]
General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]
Inhibitor: NA
Structure determination priority: 6.0
TargetDB status: NA
Availability: NA
References: 8969506; 9384377 [H]