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The map label for this gene is ntcA [H]

Identifier: 187933066

GI number: 187933066

Start: 706861

End: 707538

Strand: Reverse

Name: ntcA [H]

Synonym: CLL_A0705

Alternate gene names: 187933066

Gene position: 707538-706861 (Counterclockwise)

Preceding gene: 187933448

Following gene: 187935588

Centisome position: 18.62

GC content: 28.17

Gene sequence:

>678_bases
ATGAATAATAAACTAAAATTTTTTCCCAGTTCAACTGTAAATATAACATCTGAATTTTATAAAGTATTTGAAGATGCCGG
TACACTAAAAAAATATCGTAAAAATGAAATAATATATTTCCAAGACGATATAGCTGAAAATTTCTATTTAATTAAATCAG
GAAGAGTTCGAGTTTTTTTAATATCTCCTCAAGGTACAGAACTTACTATTGAAATTTTAAGGAAAGGAAATCTTTTTGGT
GAATCATCTTATTTTGCCTACGGTTCTAGAATGACCTCTGGTAGCGCTGCTACAGATGTGGAATTAATCGCTGTAAGCCT
AAGTGATCTTTATCCTTATCTAACAAAATATCCTGAATTAATGATTCAAATGTTCCATCTTATGTCACTATCCATTCAGA
ATTTATCAATTCAAGTTACTAGCATGGCTTTTTTAGAAGCCGATAAAAAAACCGCTGAATTATTAGTACGTTTAGGTGTG
TATTTTAAGAAAAATAAATGTGATAATTCCTACACTATTGATTATTCGCATGAAGAGATTGCTCAATTAATTGGATGTTG
TAGAGTTACTGTAACCAAAATATTAAATAGATTTCAAAAGGATGGATTAGTATCTTTAGGATATAAAGAAGTGAAAATTA
TAGATGAAAAAAAACTGAAAGAACTTGTTTTTTATTAA

Upstream 100 bases:

>100_bases
TATTCGTTTTTCCTTACAATATTTTAACTAGATACTTGAAAATAGATTGTAGTATAGGCTACAATCTATTTAATAAATAT
TTTTTATAGATGGTGATTTT

Downstream 100 bases:

>100_bases
ATTCATTTGAATAATCTATGATTATATTAAATACCTGACTATTATTATTACTAAAATCCGTTGTTTTAGGTATAACCTTA
ACTTTTTCATTAATTGAAGT

Product: CRP

Products: NA

Alternate protein names: DNA-binding protein VF1; Nitrogen-responsive regulatory protein [H]

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MNNKLKFFPSSTVNITSEFYKVFEDAGTLKKYRKNEIIYFQDDIAENFYLIKSGRVRVFLISPQGTELTIEILRKGNLFG
ESSYFAYGSRMTSGSAATDVELIAVSLSDLYPYLTKYPELMIQMFHLMSLSIQNLSIQVTSMAFLEADKKTAELLVRLGV
YFKKNKCDNSYTIDYSHEEIAQLIGCCRVTVTKILNRFQKDGLVSLGYKEVKIIDEKKLKELVFY

Sequences:

>Translated_225_residues
MNNKLKFFPSSTVNITSEFYKVFEDAGTLKKYRKNEIIYFQDDIAENFYLIKSGRVRVFLISPQGTELTIEILRKGNLFG
ESSYFAYGSRMTSGSAATDVELIAVSLSDLYPYLTKYPELMIQMFHLMSLSIQNLSIQVTSMAFLEADKKTAELLVRLGV
YFKKNKCDNSYTIDYSHEEIAQLIGCCRVTVTKILNRFQKDGLVSLGYKEVKIIDEKKLKELVFY
>Mature_225_residues
MNNKLKFFPSSTVNITSEFYKVFEDAGTLKKYRKNEIIYFQDDIAENFYLIKSGRVRVFLISPQGTELTIEILRKGNLFG
ESSYFAYGSRMTSGSAATDVELIAVSLSDLYPYLTKYPELMIQMFHLMSLSIQNLSIQVTSMAFLEADKKTAELLVRLGV
YFKKNKCDNSYTIDYSHEEIAQLIGCCRVTVTKILNRFQKDGLVSLGYKEVKIIDEKKLKELVFY

Specific function: Has affinity for the xisA upstream region. Binds to a 66 bp region containing three repeats of the consensus recognition sequence 5'-ACATT-3' [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789756, Length=178, Percent_Identity=25.8426966292135, Blast_Score=74, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR022299
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 25879; Mature: 25879

Theoretical pI: Translated: 8.68; Mature: 8.68

Prosite motif: PS50042 CNMP_BINDING_3 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNKLKFFPSSTVNITSEFYKVFEDAGTLKKYRKNEIIYFQDDIAENFYLIKSGRVRVFL
CCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEECCCEEEEE
ISPQGTELTIEILRKGNLFGESSYFAYGSRMTSGSAATDVELIAVSLSDLYPYLTKYPEL
ECCCCCEEEEEEEECCCCCCCCCEEEECCEECCCCCCCCCEEEEEEHHHHHHHHHHHHHH
MIQMFHLMSLSIQNLSIQVTSMAFLEADKKTAELLVRLGVYFKKNKCDNSYTIDYSHEEI
HHHHHHHHHHHHCCEEEEEEEEEEHHCCHHHHHHHHHHHHHEECCCCCCCEEEEECHHHH
AQLIGCCRVTVTKILNRFQKDGLVSLGYKEVKIIDEKKLKELVFY
HHHHHHHHHHHHHHHHHHHHCCCEECCCCEEEEECHHHHHHHHCC
>Mature Secondary Structure
MNNKLKFFPSSTVNITSEFYKVFEDAGTLKKYRKNEIIYFQDDIAENFYLIKSGRVRVFL
CCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEECCCEEEEE
ISPQGTELTIEILRKGNLFGESSYFAYGSRMTSGSAATDVELIAVSLSDLYPYLTKYPEL
ECCCCCEEEEEEEECCCCCCCCCEEEECCEECCCCCCCCCEEEEEEHHHHHHHHHHHHHH
MIQMFHLMSLSIQNLSIQVTSMAFLEADKKTAELLVRLGVYFKKNKCDNSYTIDYSHEEI
HHHHHHHHHHHHCCEEEEEEEEEEHHCCHHHHHHHHHHHHHEECCCCCCCEEEEECHHHH
AQLIGCCRVTVTKILNRFQKDGLVSLGYKEVKIIDEKKLKELVFY
HHHHHHHHHHHHHHHHHHHHCCCEECCCCEEEEECHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11114927 [H]