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The map label for this gene is pit [H]

Identifier: 187932989

GI number: 187932989

Start: 3745959

End: 3746957

Strand: Reverse

Name: pit [H]

Synonym: CLL_A3538

Alternate gene names: 187932989

Gene position: 3746957-3745959 (Counterclockwise)

Preceding gene: 187935722

Following gene: 187932438

Centisome position: 98.6

GC content: 31.13

Gene sequence:

>999_bases
ATGCATAGTTCGTTAATTGTAACAATACTAATAATAATTTTTGCGGTAACATTTGACTTTATAAATGGATTTCATGATAC
AGCTACAGCGGTTGCAACTTCAATAACTACAAGGGCACTTACTCCTAAACAAGCTATAATAATATGTTGTATATTTAATT
TTTTAGGTGCCTTTATGGGGACAGCAGTTGCAAAAACAGTTGGTGAAAATATTGTAAGTCATACATCTATACCACAATGG
GTAATAATGTGTGTATTAATTTCATCTATAATTTGGAATTTGCTAACATGGTATTTTGGAATTCCAAGTAGTTCATCTCA
TGCTTTAATAGGAGCACTTGTAGGAGCAGGAATTGCATATACTAGTACTCTTAATGTTGTTAATTGGTACAATCTTTTTC
ATAGCGTAATTTTATGGTTATTTCTTTCACCTGTAATAGGCTTTATAGTTGGATATATATTGATGGTAATATTAAATTTT
GCATTAAAGTCTCATAAGAGAACTGTTGTAAATAAGATATTTTTAAAATTACAAGTATTAGCTGGAGCATTTATGGCATT
TAACCATGGTGGAAATGATGCACAAAAATCTATGGGAATAATAACGATGGCATTACTAAGTGGTGGGTTAATAAGCACAT
TTGAAGTGCCTGCATGGGTAATTTTTATTTGTGCATTATCAATGGCACTTGGAACTTCTATAGGTGGTAAAAAGATAATA
AAAACTATGGGAAGTGGAATGGCAAAATTAACTCCGGTAAATGGATTTGCAGCTCAAACTGGTGCTGCTTGTGTAATATT
AACAGCAACATTATTTCATGCACCAGTAAGTACAACACATATAATAACAACAACCATAATGGGGGTTGGAGCATCTAAAA
GGTTTAAAAATGTAAAATGGGGAGTAGCTAAACAAATAGTTTGGGCATGGGTTTTAACTATTCCAATAACAGCAATATTT
TCAGCATTACTTATAGTAATAATAAAATTATTTATATAA

Upstream 100 bases:

>100_bases
ATTGAAATTATGAAATGGAAAGAAATATATCAAATTTTAGAAGATACAGTGGATAATTGTGAAAAAGTGGCTAATATAGT
AGAAAGAGTTGTAATTAAAA

Downstream 100 bases:

>100_bases
TAATAAATACATAACATATGCAATAAAAACAATTTTATAGCTATATAATTAAAAGTGCTATCTTTGAAATAAAATGTACC
TATGATTTAAGTACATTTCA

Product: phosphate permease

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MHSSLIVTILIIIFAVTFDFINGFHDTATAVATSITTRALTPKQAIIICCIFNFLGAFMGTAVAKTVGENIVSHTSIPQW
VIMCVLISSIIWNLLTWYFGIPSSSSHALIGALVGAGIAYTSTLNVVNWYNLFHSVILWLFLSPVIGFIVGYILMVILNF
ALKSHKRTVVNKIFLKLQVLAGAFMAFNHGGNDAQKSMGIITMALLSGGLISTFEVPAWVIFICALSMALGTSIGGKKII
KTMGSGMAKLTPVNGFAAQTGAACVILTATLFHAPVSTTHIITTTIMGVGASKRFKNVKWGVAKQIVWAWVLTIPITAIF
SALLIVIIKLFI

Sequences:

>Translated_332_residues
MHSSLIVTILIIIFAVTFDFINGFHDTATAVATSITTRALTPKQAIIICCIFNFLGAFMGTAVAKTVGENIVSHTSIPQW
VIMCVLISSIIWNLLTWYFGIPSSSSHALIGALVGAGIAYTSTLNVVNWYNLFHSVILWLFLSPVIGFIVGYILMVILNF
ALKSHKRTVVNKIFLKLQVLAGAFMAFNHGGNDAQKSMGIITMALLSGGLISTFEVPAWVIFICALSMALGTSIGGKKII
KTMGSGMAKLTPVNGFAAQTGAACVILTATLFHAPVSTTHIITTTIMGVGASKRFKNVKWGVAKQIVWAWVLTIPITAIF
SALLIVIIKLFI
>Mature_332_residues
MHSSLIVTILIIIFAVTFDFINGFHDTATAVATSITTRALTPKQAIIICCIFNFLGAFMGTAVAKTVGENIVSHTSIPQW
VIMCVLISSIIWNLLTWYFGIPSSSSHALIGALVGAGIAYTSTLNVVNWYNLFHSVILWLFLSPVIGFIVGYILMVILNF
ALKSHKRTVVNKIFLKLQVLAGAFMAFNHGGNDAQKSMGIITMALLSGGLISTFEVPAWVIFICALSMALGTSIGGKKII
KTMGSGMAKLTPVNGFAAQTGAACVILTATLFHAPVSTTHIITTTIMGVGASKRFKNVKWGVAKQIVWAWVLTIPITAIF
SALLIVIIKLFI

Specific function: Low-affinity inorganic phosphate transport [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Homo sapiens, GI31543630, Length=172, Percent_Identity=29.0697674418605, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI5803173, Length=162, Percent_Identity=31.4814814814815, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1789360, Length=221, Percent_Identity=30.7692307692308, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI1789907, Length=217, Percent_Identity=32.258064516129, Blast_Score=110, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17539280, Length=164, Percent_Identity=30.4878048780488, Blast_Score=94, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI25146401, Length=171, Percent_Identity=31.5789473684211, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71981576, Length=163, Percent_Identity=30.0613496932515, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI32566716, Length=158, Percent_Identity=29.746835443038, Blast_Score=81, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17536725, Length=174, Percent_Identity=25.2873563218391, Blast_Score=75, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6319773, Length=163, Percent_Identity=30.0613496932515, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21356511, Length=193, Percent_Identity=26.9430051813472, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 35654; Mature: 35654

Theoretical pI: Translated: 10.48; Mature: 10.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSSLIVTILIIIFAVTFDFINGFHDTATAVATSITTRALTPKQAIIICCIFNFLGAFMG
CCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
TAVAKTVGENIVSHTSIPQWVIMCVLISSIIWNLLTWYFGIPSSSSHALIGALVGAGIAY
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHH
TSTLNVVNWYNLFHSVILWLFLSPVIGFIVGYILMVILNFALKSHKRTVVNKIFLKLQVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGAFMAFNHGGNDAQKSMGIITMALLSGGLISTFEVPAWVIFICALSMALGTSIGGKKII
HHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
KTMGSGMAKLTPVNGFAAQTGAACVILTATLFHAPVSTTHIITTTIMGVGASKRFKNVKW
HHHCCCCEEECCCCCCHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHCCHH
GVAKQIVWAWVLTIPITAIFSALLIVIIKLFI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MHSSLIVTILIIIFAVTFDFINGFHDTATAVATSITTRALTPKQAIIICCIFNFLGAFMG
CCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
TAVAKTVGENIVSHTSIPQWVIMCVLISSIIWNLLTWYFGIPSSSSHALIGALVGAGIAY
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHH
TSTLNVVNWYNLFHSVILWLFLSPVIGFIVGYILMVILNFALKSHKRTVVNKIFLKLQVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGAFMAFNHGGNDAQKSMGIITMALLSGGLISTFEVPAWVIFICALSMALGTSIGGKKII
HHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
KTMGSGMAKLTPVNGFAAQTGAACVILTATLFHAPVSTTHIITTTIMGVGASKRFKNVKW
HHHCCCCEEECCCCCCHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHCCHH
GVAKQIVWAWVLTIPITAIFSALLIVIIKLFI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]