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The map label for this gene is dacF [H]

Identifier: 187932841

GI number: 187932841

Start: 2458174

End: 2459364

Strand: Reverse

Name: dacF [H]

Synonym: CLL_A2367

Alternate gene names: 187932841

Gene position: 2459364-2458174 (Counterclockwise)

Preceding gene: 187933384

Following gene: 187933873

Centisome position: 64.71

GC content: 29.3

Gene sequence:

>1191_bases
ATGAAAAATAATATAAAAAGAATAATATCTTTTACATTGATTTTTGTTTTAACTATTATATTAATTCCAATAGGAGCTGT
AAATGCATCTATGTTAGAGGATGAAGTTAAGGCTGATGGGATGAATGTAGAAGCAAAATCAGCATTGTTAATTGAACCAA
CAACAGGAAAAGTTGTGTATGAAAAAAATCCGGATGAAAAGTTTGCACCAGCATCTGTAACTAAAATCATGACAATGCTT
CTTACAATGGAAGCGATAGATAATGGTAAAATTAAATTAGATGATAAAGTAACTTGTAGTGAAAATGCAAAAAAAATGGG
TGGAAGTACAATGTTACTAGATACAGGTGAAATAAGAAGTGTTGAGGAATTATTAAAAGGTGTAGCTATAGCATCTGGAA
ATGATGCAGCAGTTGCTTTAGCTGAATATTTAGGTGGAACAGAATCAGATTTTGTTGGATTAATGAATAAAAGAGCTCAA
GAACTAGGAATGAAAAATACTGTATTCAAAAACTGTAATGGACTTCCAGCAGATGGACATTTATCAACAGCACGTGATAT
TTCTTTAATGTCTATTGAGCTATTAAAACATCCAACTATATTAAAATACACAGGAACTTATATGGATTCTATTTCAGAAG
GAAGAAAATCACCAATTGAACTAGTGAATCATAATAAATTAGTTAGATTTTTTGAAGGTTGTGATGGACTAAAAACAGGA
TTCACAAATGAAGCAAAATATTGTATTTCTGCAACAGCAACTAGAAATGGAGTAAGAATGCTTACTGTAATTATGGGAGC
ACCAACTTATAAAATAAGAAATAGAGATGCAGGAATACTATTAAACCATGGCTTTTCAAAATATGAAGGTAAAAAAATAG
TATCTAAAGACGAAGATGTCGAAAAAGTCCTTATGGATAAGCAAACAGATAGATTCTTTATGGCAAAAGCAAAAGATGAT
TTAACAGCAATATTACCTAAAGGAAGCAATGGCGAAATTTCTAATAAAATAGTTATTGAAGAATTGAAACCTGAATATAA
AATGGGTGATATAGTAGGAAAATGTGAAGTTTATTTAGGTGAAGAAAAAGTAGGAGAAGTAGAAATATACTCTGATAGAG
ATATAAAGAAGGGTAATTTATTTGATAATATAAAATATAATATCAAAAACTTATTTGAAAAAGGAGTATAA

Upstream 100 bases:

>100_bases
AAATAATTTAAAAATCTTGTCACATAATAAGGAATTTTATAGTTTAATAATAATATTATTTATAATTTTGGTAACACTAA
TTTTGAAGGGAGGAAGATTG

Downstream 100 bases:

>100_bases
TAAGAATTTTTAAGAGATAGATTTTAGCATAGTGTTTATAATACATTAAATTTTAAGGGAAGTTATTTTTAACTTCCCTT
AATTTACATTTTTTACTATT

Product: penicillin-binding protein DacF

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein dacF; PBP [H]

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MKNNIKRIISFTLIFVLTIILIPIGAVNASMLEDEVKADGMNVEAKSALLIEPTTGKVVYEKNPDEKFAPASVTKIMTML
LTMEAIDNGKIKLDDKVTCSENAKKMGGSTMLLDTGEIRSVEELLKGVAIASGNDAAVALAEYLGGTESDFVGLMNKRAQ
ELGMKNTVFKNCNGLPADGHLSTARDISLMSIELLKHPTILKYTGTYMDSISEGRKSPIELVNHNKLVRFFEGCDGLKTG
FTNEAKYCISATATRNGVRMLTVIMGAPTYKIRNRDAGILLNHGFSKYEGKKIVSKDEDVEKVLMDKQTDRFFMAKAKDD
LTAILPKGSNGEISNKIVIEELKPEYKMGDIVGKCEVYLGEEKVGEVEIYSDRDIKKGNLFDNIKYNIKNLFEKGV

Sequences:

>Translated_396_residues
MKNNIKRIISFTLIFVLTIILIPIGAVNASMLEDEVKADGMNVEAKSALLIEPTTGKVVYEKNPDEKFAPASVTKIMTML
LTMEAIDNGKIKLDDKVTCSENAKKMGGSTMLLDTGEIRSVEELLKGVAIASGNDAAVALAEYLGGTESDFVGLMNKRAQ
ELGMKNTVFKNCNGLPADGHLSTARDISLMSIELLKHPTILKYTGTYMDSISEGRKSPIELVNHNKLVRFFEGCDGLKTG
FTNEAKYCISATATRNGVRMLTVIMGAPTYKIRNRDAGILLNHGFSKYEGKKIVSKDEDVEKVLMDKQTDRFFMAKAKDD
LTAILPKGSNGEISNKIVIEELKPEYKMGDIVGKCEVYLGEEKVGEVEIYSDRDIKKGNLFDNIKYNIKNLFEKGV
>Mature_396_residues
MKNNIKRIISFTLIFVLTIILIPIGAVNASMLEDEVKADGMNVEAKSALLIEPTTGKVVYEKNPDEKFAPASVTKIMTML
LTMEAIDNGKIKLDDKVTCSENAKKMGGSTMLLDTGEIRSVEELLKGVAIASGNDAAVALAEYLGGTESDFVGLMNKRAQ
ELGMKNTVFKNCNGLPADGHLSTARDISLMSIELLKHPTILKYTGTYMDSISEGRKSPIELVNHNKLVRFFEGCDGLKTG
FTNEAKYCISATATRNGVRMLTVIMGAPTYKIRNRDAGILLNHGFSKYEGKKIVSKDEDVEKVLMDKQTDRFFMAKAKDD
LTAILPKGSNGEISNKIVIEELKPEYKMGDIVGKCEVYLGEEKVGEVEIYSDRDIKKGNLFDNIKYNIKNLFEKGV

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=370, Percent_Identity=33.2432432432432, Blast_Score=174, Evalue=8e-45,
Organism=Escherichia coli, GI1786851, Length=376, Percent_Identity=31.1170212765957, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI87082022, Length=356, Percent_Identity=29.2134831460674, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI87082054, Length=265, Percent_Identity=28.3018867924528, Blast_Score=85, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 43638; Mature: 43638

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNNIKRIISFTLIFVLTIILIPIGAVNASMLEDEVKADGMNVEAKSALLIEPTTGKVVY
CCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEECCCEEEEECCCCEEEE
EKNPDEKFAPASVTKIMTMLLTMEAIDNGKIKLDDKVTCSENAKKMGGSTMLLDTGEIRS
ECCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCEECCCHHHHCCCCEEEEECCCHHH
VEELLKGVAIASGNDAAVALAEYLGGTESDFVGLMNKRAQELGMKNTVFKNCNGLPADGH
HHHHHHCCEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCC
LSTARDISLMSIELLKHPTILKYTGTYMDSISEGRKSPIELVNHNKLVRFFEGCDGLKTG
CCCHHCCHHEEHHHHCCCEEEEECCHHHHHHHHCCCCCHHHHCCCHHHHHHHCCCCCCCC
FTNEAKYCISATATRNGVRMLTVIMGAPTYKIRNRDAGILLNHGFSKYEGKKIVSKDEDV
CCCCCCEEEEECCCCCCEEEEEEEECCCCEEEECCCCCEEEECCCCCCCCCCCCCCCHHH
EKVLMDKQTDRFFMAKAKDDLTAILPKGSNGEISNKIVIEELKPEYKMGDIVGKCEVYLG
HHHHHCCCCCCEEEEECCCCEEEEECCCCCCCCCCEEEEECCCCCCHHCCEEEEEEEEEC
EEKVGEVEIYSDRDIKKGNLFDNIKYNIKNLFEKGV
CCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKNNIKRIISFTLIFVLTIILIPIGAVNASMLEDEVKADGMNVEAKSALLIEPTTGKVVY
CCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEECCCEEEEECCCCEEEE
EKNPDEKFAPASVTKIMTMLLTMEAIDNGKIKLDDKVTCSENAKKMGGSTMLLDTGEIRS
ECCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCEECCCHHHHCCCCEEEEECCCHHH
VEELLKGVAIASGNDAAVALAEYLGGTESDFVGLMNKRAQELGMKNTVFKNCNGLPADGH
HHHHHHCCEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCC
LSTARDISLMSIELLKHPTILKYTGTYMDSISEGRKSPIELVNHNKLVRFFEGCDGLKTG
CCCHHCCHHEEHHHHCCCEEEEECCHHHHHHHHCCCCCHHHHCCCHHHHHHHCCCCCCCC
FTNEAKYCISATATRNGVRMLTVIMGAPTYKIRNRDAGILLNHGFSKYEGKKIVSKDEDV
CCCCCCEEEEECCCCCCEEEEEEEECCCCEEEECCCCCEEEECCCCCCCCCCCCCCCHHH
EKVLMDKQTDRFFMAKAKDDLTAILPKGSNGEISNKIVIEELKPEYKMGDIVGKCEVYLG
HHHHHCCCCCCEEEEECCCCEEEEECCCCCCCCCCEEEEECCCCCCHHCCEEEEEEEEEC
EEKVGEVEIYSDRDIKKGNLFDNIKYNIKNLFEKGV
CCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629150; 8969508; 9384377 [H]