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The map label for this gene is murE [H]

Identifier: 187932581

GI number: 187932581

Start: 2527821

End: 2529275

Strand: Reverse

Name: murE [H]

Synonym: CLL_A2445

Alternate gene names: 187932581

Gene position: 2529275-2527821 (Counterclockwise)

Preceding gene: 187933438

Following gene: 187935120

Centisome position: 66.55

GC content: 27.84

Gene sequence:

>1455_bases
ATGAACCTTAAAGATATTTTAAAGGGAATAGACTATAAGCTTGTACAAGGAAGCTTAGATAAAGAAATATCTTCAATTAA
TTATGATAGTAGAAAGGTTAATAAGTCAGATATTTTTATCTGTATAAAAGGGTATGCTACTGATGGGCATAAATATATTC
AAAAGGCAATTGAAAATGGCGCCTCTGTTATTATAATGCAAGATGATATTGAAGTAGTAAATAAAAATATATCTGTTATT
AAAGTAAAGGATACTAGAAAAGCGTTAGCTCTTATAGGAATAAATTATTACGATAATCCAAGTAAAAAAATGAAGATTAT
TGGTATAACAGGCACTAATGGCAAAACGACAACTGCATTTATGATCAAATCAATTCTAGAATCCAATGGTAAAAAGGTAG
GACTTATAGGAACTATAGCTAATTACATTGGAAATGAAAAACTTCATACAGAAAGAACTACACCTGAATCATTAGAATTA
CAAGAATTGTTCAATAATATGGTTAATAAGGGTGTAGAATACTGCGTTATGGAAGTATCTTCTCATTCTCTTGAATTAGA
TAGAGTATATGGAGTTGACTTTGAGGTTGGAATTTTTACGAATTTAACGAGAGATCATTTAGATTTCCATAAAACTTTTG
AAAATTATTACAATTCTAAATTTAAATTATTTAAGAGAAGTAATATAAGAATAATTAATAATGATGACAATTATGGAAAG
CAAGTAATTAAAGATTTAAATAAACTGAAAGCAAAAAATATATATGAATATTCTATAAAGGAAGACTCTAATTTTAAAGC
ATTTGATGAAAAAATGGGAAGTAAAAACATAAGTTTTAAAGTTAATTTAGAAAAAGAGGAAGAATTTATTTTATCAATAC
CAGGCGAGTACAATATATATAATGCCTTAGGTGCTATAGGAGCATGCTATAAGTTAAATATACCAATGGAAGCTATAAAA
AACGGAATTAACAAAGTTATAGTTCCAGGTAGATGTGAAAGAACAGCTACTAATTACAATTTACCTTATGACATAATAAT
AGATTATGCTCATACTCCAGATGGATTACAAAACATCTTAGAAACTGCAAGAGGATTTACGCAAGGAAGATTAATTGCCA
TCTTTGGATGTGGAGGGGATAGAGATAAGGTAAAGCGTCCTCAAATGGGTAAAATAGGAACTGATATAGCAGATATAGCT
ATAGTAACTTCAGATAACCCAAGAAGCGAAGAGCCTATGAGAATAATAGAGGATATACTAGTAGGAATAGAAAAAGATAA
TTATGAAATTATAGAAAATAGAAAAGATGCTATAAAAAAAGCAATGGATATAGCCAAAGAAAATGATGTAATAGTTATAG
CTGGTAAAGGACATGAAACATATCAAATTTTAAAAGACAAAACAATACATTTTGATGAAAGAGAAGTTGTTAAACAAATA
TTAGATGATAAATAA

Upstream 100 bases:

>100_bases
GCATGTGGGTTTTGCTCACATGCTAAATTATTGTAAAATAATCTTTTATGTAGTAATTAAATAACATAATAAGCATATTA
TTTAAGGAGATGGTTAAAAA

Downstream 100 bases:

>100_bases
TAAGAGGTGAGATTATATTATGGATTTAACTCTAAATGAAGTACTAAAAGCAATTGATGGTGAATTGCTAATAAAAAATA
ATGAAGGAAATTTTGAAAAA

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MNLKDILKGIDYKLVQGSLDKEISSINYDSRKVNKSDIFICIKGYATDGHKYIQKAIENGASVIIMQDDIEVVNKNISVI
KVKDTRKALALIGINYYDNPSKKMKIIGITGTNGKTTTAFMIKSILESNGKKVGLIGTIANYIGNEKLHTERTTPESLEL
QELFNNMVNKGVEYCVMEVSSHSLELDRVYGVDFEVGIFTNLTRDHLDFHKTFENYYNSKFKLFKRSNIRIINNDDNYGK
QVIKDLNKLKAKNIYEYSIKEDSNFKAFDEKMGSKNISFKVNLEKEEEFILSIPGEYNIYNALGAIGACYKLNIPMEAIK
NGINKVIVPGRCERTATNYNLPYDIIIDYAHTPDGLQNILETARGFTQGRLIAIFGCGGDRDKVKRPQMGKIGTDIADIA
IVTSDNPRSEEPMRIIEDILVGIEKDNYEIIENRKDAIKKAMDIAKENDVIVIAGKGHETYQILKDKTIHFDEREVVKQI
LDDK

Sequences:

>Translated_484_residues
MNLKDILKGIDYKLVQGSLDKEISSINYDSRKVNKSDIFICIKGYATDGHKYIQKAIENGASVIIMQDDIEVVNKNISVI
KVKDTRKALALIGINYYDNPSKKMKIIGITGTNGKTTTAFMIKSILESNGKKVGLIGTIANYIGNEKLHTERTTPESLEL
QELFNNMVNKGVEYCVMEVSSHSLELDRVYGVDFEVGIFTNLTRDHLDFHKTFENYYNSKFKLFKRSNIRIINNDDNYGK
QVIKDLNKLKAKNIYEYSIKEDSNFKAFDEKMGSKNISFKVNLEKEEEFILSIPGEYNIYNALGAIGACYKLNIPMEAIK
NGINKVIVPGRCERTATNYNLPYDIIIDYAHTPDGLQNILETARGFTQGRLIAIFGCGGDRDKVKRPQMGKIGTDIADIA
IVTSDNPRSEEPMRIIEDILVGIEKDNYEIIENRKDAIKKAMDIAKENDVIVIAGKGHETYQILKDKTIHFDEREVVKQI
LDDK
>Mature_484_residues
MNLKDILKGIDYKLVQGSLDKEISSINYDSRKVNKSDIFICIKGYATDGHKYIQKAIENGASVIIMQDDIEVVNKNISVI
KVKDTRKALALIGINYYDNPSKKMKIIGITGTNGKTTTAFMIKSILESNGKKVGLIGTIANYIGNEKLHTERTTPESLEL
QELFNNMVNKGVEYCVMEVSSHSLELDRVYGVDFEVGIFTNLTRDHLDFHKTFENYYNSKFKLFKRSNIRIINNDDNYGK
QVIKDLNKLKAKNIYEYSIKEDSNFKAFDEKMGSKNISFKVNLEKEEEFILSIPGEYNIYNALGAIGACYKLNIPMEAIK
NGINKVIVPGRCERTATNYNLPYDIIIDYAHTPDGLQNILETARGFTQGRLIAIFGCGGDRDKVKRPQMGKIGTDIADIA
IVTSDNPRSEEPMRIIEDILVGIEKDNYEIIENRKDAIKKAMDIAKENDVIVIAGKGHETYQILKDKTIHFDEREVVKQI
LDDK

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=494, Percent_Identity=34.0080971659919, Blast_Score=267, Evalue=1e-72,
Organism=Escherichia coli, GI1786274, Length=347, Percent_Identity=23.9193083573487, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 54837; Mature: 54837

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLKDILKGIDYKLVQGSLDKEISSINYDSRKVNKSDIFICIKGYATDGHKYIQKAIENG
CCHHHHHHCCCCEEECCCHHHHHHHCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCC
ASVIIMQDDIEVVNKNISVIKVKDTRKALALIGINYYDNPSKKMKIIGITGTNGKTTTAF
CEEEEEECCHHHHCCCEEEEEEECCCCEEEEEEEECCCCCCCEEEEEEEECCCCCCHHHH
MIKSILESNGKKVGLIGTIANYIGNEKLHTERTTPESLELQELFNNMVNKGVEYCVMEVS
HHHHHHHCCCCEEEEHHHHHHHHCCCCEECCCCCCCCCHHHHHHHHHHHCCHHEEEEECC
SHSLELDRVYGVDFEVGIFTNLTRDHLDFHKTFENYYNSKFKLFKRSNIRIINNDDNYGK
CCCEEEEEEECCEEEEEEECCCCHHHHHHHHHHHHHCCCEEEEEECCCEEEEECCCHHHH
QVIKDLNKLKAKNIYEYSIKEDSNFKAFDEKMGSKNISFKVNLEKEEEFILSIPGEYNIY
HHHHHHHHHHHCCEEEEEECCCCCCHHHHHHCCCCCEEEEEEECCCCEEEEECCCCCCHH
NALGAIGACYKLNIPMEAIKNGINKVIVPGRCERTATNYNLPYDIIIDYAHTPDGLQNIL
HHHHHHCCEEEECCCHHHHHCCCCEEECCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHH
ETARGFTQGRLIAIFGCGGDRDKVKRPQMGKIGTDIADIAIVTSDNPRSEEPMRIIEDIL
HHHCCCCCCCEEEEEECCCCHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHH
VGIEKDNYEIIENRKDAIKKAMDIAKENDVIVIAGKGHETYQILKDKTIHFDEREVVKQI
HCCCCCCHHHHHCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHCCCCCCCCHHHHHHHH
LDDK
HCCC
>Mature Secondary Structure
MNLKDILKGIDYKLVQGSLDKEISSINYDSRKVNKSDIFICIKGYATDGHKYIQKAIENG
CCHHHHHHCCCCEEECCCHHHHHHHCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCC
ASVIIMQDDIEVVNKNISVIKVKDTRKALALIGINYYDNPSKKMKIIGITGTNGKTTTAF
CEEEEEECCHHHHCCCEEEEEEECCCCEEEEEEEECCCCCCCEEEEEEEECCCCCCHHHH
MIKSILESNGKKVGLIGTIANYIGNEKLHTERTTPESLELQELFNNMVNKGVEYCVMEVS
HHHHHHHCCCCEEEEHHHHHHHHCCCCEECCCCCCCCCHHHHHHHHHHHCCHHEEEEECC
SHSLELDRVYGVDFEVGIFTNLTRDHLDFHKTFENYYNSKFKLFKRSNIRIINNDDNYGK
CCCEEEEEEECCEEEEEEECCCCHHHHHHHHHHHHHCCCEEEEEECCCEEEEECCCHHHH
QVIKDLNKLKAKNIYEYSIKEDSNFKAFDEKMGSKNISFKVNLEKEEEFILSIPGEYNIY
HHHHHHHHHHHCCEEEEEECCCCCCHHHHHHCCCCCEEEEEEECCCCEEEEECCCCCCHH
NALGAIGACYKLNIPMEAIKNGINKVIVPGRCERTATNYNLPYDIIIDYAHTPDGLQNIL
HHHHHHCCEEEECCCHHHHHCCCCEEECCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHH
ETARGFTQGRLIAIFGCGGDRDKVKRPQMGKIGTDIADIAIVTSDNPRSEEPMRIIEDIL
HHHCCCCCCCEEEEEECCCCHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHH
VGIEKDNYEIIENRKDAIKKAMDIAKENDVIVIAGKGHETYQILKDKTIHFDEREVVKQI
HCCCCCCHHHHHCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHCCCCCCCCHHHHHHHH
LDDK
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11792842 [H]