| Definition | Clostridium botulinum B str. Eklund 17B, complete genome. |
|---|---|
| Accession | NC_010674 |
| Length | 3,800,327 |
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The map label for this gene is spoVD [H]
Identifier: 187933438
GI number: 187933438
Start: 2529401
End: 2531659
Strand: Reverse
Name: spoVD [H]
Synonym: CLL_A2446
Alternate gene names: 187933438
Gene position: 2531659-2529401 (Counterclockwise)
Preceding gene: 187934253
Following gene: 187932581
Centisome position: 66.62
GC content: 32.58
Gene sequence:
>2259_bases GTGAAAAAACTAAATTATAAAGATAGAGCAAAAATGCGCAAAAGAATGACTATAATTATTGCGGGATTAAGTTGTGTATT TTTAGTGTTAAGTATTAGACTATCTTACATAATGATAGCTAAAAGACAAGAATATGCAGCAAGAGCAGAAGAACAGTGGA CAAGCGAAGTGAAAATAGATGCAAGAAGAGGAAGAATATTAGACAGAAATGGTAAAGAATTAGCTGTATCTGCTAATGTA TATAGAGTTGATTTTGACTTAAACTCTATAAGATCTTTTTTAAGGCAAGATTTAACTGAAAAAAGGCGTGAAAAGCTTAG TAGTGTTGGAATTAATGTTAAACCAGGACAGGAAGCCTTAACAACTGCTGATATTGCTCCTGTTATTGCAAGTGCTCTAG ATTTAGATTCAGAAAAAGTAAAAGAAAAACTAGAAACTAAGCTCCCAAGTGGAGCAGATGCTGGTTCAGCAACACTTGTA AGAAGAATTGAAAAAGATGCTGCTGATAAAGTAAGAGATTTAAATATTAGTGGCGTTTTGGTATCGCCAGATACTAAAAG GTATTATCCAAATAATAACTTTTTATCACATGTTCTTGGAAGTACTAATATTGATGGTCAAGGGTTAACTGGAGTAGAAG TACAATATAATAATTATTTGTCTGGAGTACCTGGAATGAAAATTTCAGAATTAGATAGAAATAGTGGAGAACTACCATAT ACAATATCACAATTCACACCTCCAGTAGATGGAAAAGATATAACTTTAACGATTGATGAGAATATTCAGTTCTTTGCTGA AAAGGCAGCACAGCAAACATATGAAGATAATAAAGCTAAAGCAGTGTCTATATTAGTTATGGATCCTAAAAGTGGAGAGG TTTTAGCTATGGTTAATAAACCTGACTTTAACCCTAATGCCCCATATGAAGGTACTGATAACTTTGACGGTGCAAATGAA TCGGAAAGATTACAAAAGATGTGGAGAAATAGGCTTGTAAATGATACGTTTGAACCAGGATCTATTTTTAAGGTCGTAAC AGCAATTACTGCATTAGAAGAGAATATTGTAAATGCAAATACTGATTTTACATGTGGTGGTGGTTTACACTTTGGAAATA GATATATAAAGTGTTGGAGAACACAAGGACATGGAGCGCAAAAATTCCCAGATATAATTCAAAATTCATGTAATGTTGGT TTTATGAAGCTTGGAGAAATGATAGGAAAAGAAAAATTATGTGAATATATTGAAAAGTTTGGATTTGGAAAGGTAAGTGG AATTGATTTACCAGGAGAAGCTAAAGGAATTGTTAAAAAAGTTGATAAAATTTCAGAAACAGATTTAGCAACTATAGCTT TTGGACAAACTAATACTGTAAATTCAGTTCAATATATGACAGCCTTTAATGCTGTTGCAAATGGTGGTCAATTAATACAA CCACATGTTATGAAGGAAATTACTCATAGCGGTGATAATGGAGTTAAAATAGTTGATGAATCATTTAATTCTAAGGTTAC TGATGTAGCAACTAAAGAAAAAACAGCTGAACTTAGAAGTTATCTTGAAAGAGTTGTAACTGGAGGATCTGGAGCAGGAA CATTTATGGAAGGATATCACATTGGAGGAAAAACAGGAACAGCTCAAAAAGTTAATCCTGAAAATGGTACTTATGGTGCT GGACAGTATATATCATCTTTTGTTGGTATGGCACCAGTAGATGATCCTAAGATAACTGTGATGGTAACAGTAGATGAGCC GAGTAATGGTGCGTATTATGCAGGTCAAGTATGTACACCAGCAGCTAAAATGCTATTCTCTGATATATTTAATTATTTAG ACAGTAAATTTTCTGATGAAAATTTAAGTCAAATATCTAGAGATGTTATTATTCCAGAAGTTAGAGGCATGAAGGTTGAT GAAGCAAAGAAAGTACTAAAAGAATCTAAATTAAATTTTTCTATAGATGAATCAGGTGATACTGTTATAGATATGAAGCC ATATCCAGGATATGCAGTAAAGGAAGAATCTGAAATAAATCTTTACACAGGTAACAGTGGGTCTTATAATAAAAATGTTG TTATGCCTGATGTAAGAGGATATTCTAAAGATGATGCTAATCAATTATTAACTAGTATTGGAATAGCACCTATCTTCCAA GGTGGAGGTATGGTAATAGAACAAAATGTTAATCCAGGAGAGGTTATAACTAAGGGCACATCGGTTAAATTAACTTTAAA TTCAGATTATAAAGACTAA
Upstream 100 bases:
>100_bases AAGTATTTTTTAGACTAAATTATAAGGGGATTTTTAATTACATTACAGTTTAAAAAAGAGACACTAATCTAATAGAAATT ATATGGAGGAGTTATAAGTA
Downstream 100 bases:
>100_bases TTAGAATACTTAAGTTAAATTTTTAGCATGTGGGTTTTGCTCACATGCTAAATTATTGTAAAATAATCTTTTATGTAGTA ATTAAATAACATAATAAGCA
Product: stage V sporulation protein D
Products: NA
Alternate protein names: Sporulation-specific penicillin-binding protein [H]
Number of amino acids: Translated: 752; Mature: 752
Protein sequence:
>752_residues MKKLNYKDRAKMRKRMTIIIAGLSCVFLVLSIRLSYIMIAKRQEYAARAEEQWTSEVKIDARRGRILDRNGKELAVSANV YRVDFDLNSIRSFLRQDLTEKRREKLSSVGINVKPGQEALTTADIAPVIASALDLDSEKVKEKLETKLPSGADAGSATLV RRIEKDAADKVRDLNISGVLVSPDTKRYYPNNNFLSHVLGSTNIDGQGLTGVEVQYNNYLSGVPGMKISELDRNSGELPY TISQFTPPVDGKDITLTIDENIQFFAEKAAQQTYEDNKAKAVSILVMDPKSGEVLAMVNKPDFNPNAPYEGTDNFDGANE SERLQKMWRNRLVNDTFEPGSIFKVVTAITALEENIVNANTDFTCGGGLHFGNRYIKCWRTQGHGAQKFPDIIQNSCNVG FMKLGEMIGKEKLCEYIEKFGFGKVSGIDLPGEAKGIVKKVDKISETDLATIAFGQTNTVNSVQYMTAFNAVANGGQLIQ PHVMKEITHSGDNGVKIVDESFNSKVTDVATKEKTAELRSYLERVVTGGSGAGTFMEGYHIGGKTGTAQKVNPENGTYGA GQYISSFVGMAPVDDPKITVMVTVDEPSNGAYYAGQVCTPAAKMLFSDIFNYLDSKFSDENLSQISRDVIIPEVRGMKVD EAKKVLKESKLNFSIDESGDTVIDMKPYPGYAVKEESEINLYTGNSGSYNKNVVMPDVRGYSKDDANQLLTSIGIAPIFQ GGGMVIEQNVNPGEVITKGTSVKLTLNSDYKD
Sequences:
>Translated_752_residues MKKLNYKDRAKMRKRMTIIIAGLSCVFLVLSIRLSYIMIAKRQEYAARAEEQWTSEVKIDARRGRILDRNGKELAVSANV YRVDFDLNSIRSFLRQDLTEKRREKLSSVGINVKPGQEALTTADIAPVIASALDLDSEKVKEKLETKLPSGADAGSATLV RRIEKDAADKVRDLNISGVLVSPDTKRYYPNNNFLSHVLGSTNIDGQGLTGVEVQYNNYLSGVPGMKISELDRNSGELPY TISQFTPPVDGKDITLTIDENIQFFAEKAAQQTYEDNKAKAVSILVMDPKSGEVLAMVNKPDFNPNAPYEGTDNFDGANE SERLQKMWRNRLVNDTFEPGSIFKVVTAITALEENIVNANTDFTCGGGLHFGNRYIKCWRTQGHGAQKFPDIIQNSCNVG FMKLGEMIGKEKLCEYIEKFGFGKVSGIDLPGEAKGIVKKVDKISETDLATIAFGQTNTVNSVQYMTAFNAVANGGQLIQ PHVMKEITHSGDNGVKIVDESFNSKVTDVATKEKTAELRSYLERVVTGGSGAGTFMEGYHIGGKTGTAQKVNPENGTYGA GQYISSFVGMAPVDDPKITVMVTVDEPSNGAYYAGQVCTPAAKMLFSDIFNYLDSKFSDENLSQISRDVIIPEVRGMKVD EAKKVLKESKLNFSIDESGDTVIDMKPYPGYAVKEESEINLYTGNSGSYNKNVVMPDVRGYSKDDANQLLTSIGIAPIFQ GGGMVIEQNVNPGEVITKGTSVKLTLNSDYKD >Mature_752_residues MKKLNYKDRAKMRKRMTIIIAGLSCVFLVLSIRLSYIMIAKRQEYAARAEEQWTSEVKIDARRGRILDRNGKELAVSANV YRVDFDLNSIRSFLRQDLTEKRREKLSSVGINVKPGQEALTTADIAPVIASALDLDSEKVKEKLETKLPSGADAGSATLV RRIEKDAADKVRDLNISGVLVSPDTKRYYPNNNFLSHVLGSTNIDGQGLTGVEVQYNNYLSGVPGMKISELDRNSGELPY TISQFTPPVDGKDITLTIDENIQFFAEKAAQQTYEDNKAKAVSILVMDPKSGEVLAMVNKPDFNPNAPYEGTDNFDGANE SERLQKMWRNRLVNDTFEPGSIFKVVTAITALEENIVNANTDFTCGGGLHFGNRYIKCWRTQGHGAQKFPDIIQNSCNVG FMKLGEMIGKEKLCEYIEKFGFGKVSGIDLPGEAKGIVKKVDKISETDLATIAFGQTNTVNSVQYMTAFNAVANGGQLIQ PHVMKEITHSGDNGVKIVDESFNSKVTDVATKEKTAELRSYLERVVTGGSGAGTFMEGYHIGGKTGTAQKVNPENGTYGA GQYISSFVGMAPVDDPKITVMVTVDEPSNGAYYAGQVCTPAAKMLFSDIFNYLDSKFSDENLSQISRDVIIPEVRGMKVD EAKKVLKESKLNFSIDESGDTVIDMKPYPGYAVKEESEINLYTGNSGSYNKNVVMPDVRGYSKDDANQLLTSIGIAPIFQ GGGMVIEQNVNPGEVITKGTSVKLTLNSDYKD
Specific function: Penicillin-binding protein with an unknown catalytic activity. May have a specialized role in the morphogenesis of spore cortex, which is a modified form of peptidoglycan. Pore cortex formation is determined primarily by the mother cell [H]
COG id: COG0768
COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2
Gene ontology:
Cell location: Cell membrane; Peripheral membrane protein (Potential) [H]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 PASTA domain [H]
Homologues:
Organism=Escherichia coli, GI1786272, Length=601, Percent_Identity=26.955074875208, Blast_Score=211, Evalue=2e-55, Organism=Escherichia coli, GI1786854, Length=649, Percent_Identity=23.5747303543914, Blast_Score=132, Evalue=1e-31,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR012338 - InterPro: IPR005543 - InterPro: IPR005311 - InterPro: IPR001460 - InterPro: IPR011927 [H]
Pfam domain/function: PF03793 PASTA; PF03717 PBP_dimer; PF00905 Transpeptidase [H]
EC number: NA
Molecular weight: Translated: 82538; Mature: 82538
Theoretical pI: Translated: 5.57; Mature: 5.57
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.8 %Cys (Translated Protein) 2.7 %Met (Translated Protein) 3.5 %Cys+Met (Translated Protein) 0.8 %Cys (Mature Protein) 2.7 %Met (Mature Protein) 3.5 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKKLNYKDRAKMRKRMTIIIAGLSCVFLVLSIRLSYIMIAKRQEYAARAEEQWTSEVKID CCCCCCHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHCCCEEEE ARRGRILDRNGKELAVSANVYRVDFDLNSIRSFLRQDLTEKRREKLSSVGINVKPGQEAL CCCCCEECCCCCEEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH TTADIAPVIASALDLDSEKVKEKLETKLPSGADAGSATLVRRIEKDAADKVRDLNISGVL HHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEE VSPDTKRYYPNNNFLSHVLGSTNIDGQGLTGVEVQYNNYLSGVPGMKISELDRNSGELPY ECCCCCCCCCCCCHHHHHCCCCCCCCCCCEEEEEEECCEECCCCCCEEHHCCCCCCCCCE TISQFTPPVDGKDITLTIDENIQFFAEKAAQQTYEDNKAKAVSILVMDPKSGEVLAMVNK EHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCEEEEEEC PDFNPNAPYEGTDNFDGANESERLQKMWRNRLVNDTFEPGSIFKVVTAITALEENIVNAN CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCC TDFTCGGGLHFGNRYIKCWRTQGHGAQKFPDIIQNSCNVGFMKLGEMIGKEKLCEYIEKF CCEEECCCCCCCCEEEEEEECCCCCCHHHHHHHHCCCCCCHHHHHHHHCHHHHHHHHHHH GFGKVSGIDLPGEAKGIVKKVDKISETDLATIAFGQTNTVNSVQYMTAFNAVANGGQLIQ CCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEC PHVMKEITHSGDNGVKIVDESFNSKVTDVATKEKTAELRSYLERVVTGGSGAGTFMEGYH HHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEE IGGKTGTAQKVNPENGTYGAGQYISSFVGMAPVDDPKITVMVTVDEPSNGAYYAGQVCTP ECCCCCCCCEECCCCCCCCHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCEEECCCCCH AAKMLFSDIFNYLDSKFSDENLSQISRDVIIPEVRGMKVDEAKKVLKESKLNFSIDESGD HHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEECCCCC TVIDMKPYPGYAVKEESEINLYTGNSGSYNKNVVMPDVRGYSKDDANQLLTSIGIAPIFQ EEEEECCCCCCCCCCCCEEEEEECCCCCCCCCEECCCCCCCCCCHHHHHHHHHCCCEEEC GGGMVIEQNVNPGEVITKGTSVKLTLNSDYKD CCCEEEECCCCCCCEEECCCEEEEEECCCCCC >Mature Secondary Structure MKKLNYKDRAKMRKRMTIIIAGLSCVFLVLSIRLSYIMIAKRQEYAARAEEQWTSEVKID CCCCCCHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHCCCEEEE ARRGRILDRNGKELAVSANVYRVDFDLNSIRSFLRQDLTEKRREKLSSVGINVKPGQEAL CCCCCEECCCCCEEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH TTADIAPVIASALDLDSEKVKEKLETKLPSGADAGSATLVRRIEKDAADKVRDLNISGVL HHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEE VSPDTKRYYPNNNFLSHVLGSTNIDGQGLTGVEVQYNNYLSGVPGMKISELDRNSGELPY ECCCCCCCCCCCCHHHHHCCCCCCCCCCCEEEEEEECCEECCCCCCEEHHCCCCCCCCCE TISQFTPPVDGKDITLTIDENIQFFAEKAAQQTYEDNKAKAVSILVMDPKSGEVLAMVNK EHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCEEEEEEC PDFNPNAPYEGTDNFDGANESERLQKMWRNRLVNDTFEPGSIFKVVTAITALEENIVNAN CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCC TDFTCGGGLHFGNRYIKCWRTQGHGAQKFPDIIQNSCNVGFMKLGEMIGKEKLCEYIEKF CCEEECCCCCCCCEEEEEEECCCCCCHHHHHHHHCCCCCCHHHHHHHHCHHHHHHHHHHH GFGKVSGIDLPGEAKGIVKKVDKISETDLATIAFGQTNTVNSVQYMTAFNAVANGGQLIQ CCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEC PHVMKEITHSGDNGVKIVDESFNSKVTDVATKEKTAELRSYLERVVTGGSGAGTFMEGYH HHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEE IGGKTGTAQKVNPENGTYGAGQYISSFVGMAPVDDPKITVMVTVDEPSNGAYYAGQVCTP ECCCCCCCCEECCCCCCCCHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCEEECCCCCH AAKMLFSDIFNYLDSKFSDENLSQISRDVIIPEVRGMKVDEAKKVLKESKLNFSIDESGD HHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEECCCCC TVIDMKPYPGYAVKEESEINLYTGNSGSYNKNVVMPDVRGYSKDDANQLLTSIGIAPIFQ EEEEECCCCCCCCCCCCEEEEEECCCCCCCCCEECCCCCCCCCCHHHHHHHHHCCCEEEC GGGMVIEQNVNPGEVITKGTSVKLTLNSDYKD CCCEEEECCCCCCCEEECCCEEEEEECCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 6.0
TargetDB status: NA
Availability: NA
References: 8289242; 9384377; 8244929; 8436954 [H]