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The map label for this gene is yfkN [H]

Identifier: 187932571

GI number: 187932571

Start: 2933361

End: 2935109

Strand: Reverse

Name: yfkN [H]

Synonym: CLL_A2858

Alternate gene names: 187932571

Gene position: 2935109-2933361 (Counterclockwise)

Preceding gene: 187932953

Following gene: 187934123

Centisome position: 77.23

GC content: 29.5

Gene sequence:

>1749_bases
ATGAAAAAGAAGATTTTAAGTGCTATGTTATCGCTTACATTAGTTTTTAGTTTAAATACCACTAGTGTATGGGCAGCTAG
CAAAGATGATAGCACAAAAATAACAATAATTCATACAAATGATACCCATAGTAGGGTATTATCAGCAGATGGTGGGTTTG
GATTTGCAAAGATTGCAACTATAGCAAATGAAACTAAAAAAGAAAATCCAAATACATTATTAGTAGATGCTGGAGACACT
TTACATGGAAAACCAATAGTTAATGTTAGTAAAGGTGAAAATGCTGTTAAAATATTGGATGCAGCTGGTTATGATTTTAT
GGTTCCAGGTAATCATGATTTTAACTATGGAAATGAAAGGTTAATAGAATTAAGCAAATTAGCTAAAAATTTTAAAATGT
TAGATGCTAATGTAAAAAAGAATGGAAAAGATATGTTACCACCATATGAAATTATAGAAAAAGGAAATGTTAAAATAGGA
ATTTTTGGACTATCAACTCCAGAAACAGCATATAAAACAAATCCTGCTAATGTAAAAGATGTAACTTTTGAAGATCCTAT
AAAGGTATCAGAAGAGATGGTTAAAGAATTAAAAGATAAGACAGATGTTATTGTAGCGTTAGCTCATGTAGGATTAGATG
ATAGTTCAGTTGTAACATCTAAGCAAATTGCTGAAAAAGTTAAGGGAATTGATGTAATAATAGATGGACATAGCCATACA
ACTTTAAATGATGGCTTAGTAGTTAATAATACATTAATAGCACAAACAGGTGAATATGACCAAAATCTTGGTGTAGTAGA
ATTAGAAGTTAAAGATAAAAAAGTAACAGAGAAGAAAGCAAAATTATTAAACTCAAAAGATTATAAGGAATTAAAAGAAG
ATGAAAAAGTAACAGATTTATTAAATGATATACAAGCTGAAAATGACAAGGTATTTTCAGAAGTTGTTGCAAGTAATGAT
GTATTACTTGATGGTGTTAGAGGTAATGTAAGAACTAAAGAAACAAACCTTGGAAATTTAACAGCAGATGCTACTAGAGA
AGTAACTAAAGCTGATATTGCATTTGTAAATGGAGGAACTTTAAGAACTTCAATTGAACCAGGGGATATAACTAAGGGAA
AACTTGCAGAATTATTCCCATTTGGTAGTGTAGCACAAACTGTAAAATTATCAGGTAGTGATATAGTTAATGCTCTTGAA
GTATCAGTAGGGGAATATCCAACAGAACAAGGTGGATTTTTACAAGTAAGTGGAATAACATTTTCATTTGATCCAACGAA
GGAAAAAGGAAATAGAGTTTTTGATGTAAAAGTTGGTGAAAATCCAATTGATTTAAATAAGGAATATACTGTAGCAATAA
ATGATTTCTTATCTCAAGGTGGAGATGGATATACAATGCTTAAAACTACTGTTGTAGGAGAATTTGCAACATATGATGAT
ATTTTTGCAAAATATTTAAATGACAATGGTATGAAAAATGATATTAATTTAGGTAGAATACAAGTTAAAGAAAATGGTAA
ACAAGTAGTAACAAATCAAAATAACGTAGTTCCAGTTCAAGCAGTACCAACAGTACAAGAACCACAAGTATCTGCTAAAA
CAGAAACTGTATATACTGTAATTGCAGGTGATAATCTTACTAAGATAGCTAGAATAAATAATACTACATGGCAAACATTA
GCTCAATATAATAATTTACAAAACCCAGATCTAATATATGTAGGAGATCAAATTAAAATTCCAGCATAA

Upstream 100 bases:

>100_bases
AAAATAAAATTACTAAAACACAAGAAGTCCTTGAAAAAAATACAAATATATACCATAATTAAATATATAGTTACAATTGA
TCAGGGGGGGGATAATAAAT

Downstream 100 bases:

>100_bases
TTTATCTTAGTTATTTATAGAGTAGAAACATGATAAAAAATGTTTCTACTTTTTTTATGTATAGGAAATTGAAGAATTTT
TAAAGACTAGAAGGATGATG

Product: nucleotidase

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 582; Mature: 582

Protein sequence:

>582_residues
MKKKILSAMLSLTLVFSLNTTSVWAASKDDSTKITIIHTNDTHSRVLSADGGFGFAKIATIANETKKENPNTLLVDAGDT
LHGKPIVNVSKGENAVKILDAAGYDFMVPGNHDFNYGNERLIELSKLAKNFKMLDANVKKNGKDMLPPYEIIEKGNVKIG
IFGLSTPETAYKTNPANVKDVTFEDPIKVSEEMVKELKDKTDVIVALAHVGLDDSSVVTSKQIAEKVKGIDVIIDGHSHT
TLNDGLVVNNTLIAQTGEYDQNLGVVELEVKDKKVTEKKAKLLNSKDYKELKEDEKVTDLLNDIQAENDKVFSEVVASND
VLLDGVRGNVRTKETNLGNLTADATREVTKADIAFVNGGTLRTSIEPGDITKGKLAELFPFGSVAQTVKLSGSDIVNALE
VSVGEYPTEQGGFLQVSGITFSFDPTKEKGNRVFDVKVGENPIDLNKEYTVAINDFLSQGGDGYTMLKTTVVGEFATYDD
IFAKYLNDNGMKNDINLGRIQVKENGKQVVTNQNNVVPVQAVPTVQEPQVSAKTETVYTVIAGDNLTKIARINNTTWQTL
AQYNNLQNPDLIYVGDQIKIPA

Sequences:

>Translated_582_residues
MKKKILSAMLSLTLVFSLNTTSVWAASKDDSTKITIIHTNDTHSRVLSADGGFGFAKIATIANETKKENPNTLLVDAGDT
LHGKPIVNVSKGENAVKILDAAGYDFMVPGNHDFNYGNERLIELSKLAKNFKMLDANVKKNGKDMLPPYEIIEKGNVKIG
IFGLSTPETAYKTNPANVKDVTFEDPIKVSEEMVKELKDKTDVIVALAHVGLDDSSVVTSKQIAEKVKGIDVIIDGHSHT
TLNDGLVVNNTLIAQTGEYDQNLGVVELEVKDKKVTEKKAKLLNSKDYKELKEDEKVTDLLNDIQAENDKVFSEVVASND
VLLDGVRGNVRTKETNLGNLTADATREVTKADIAFVNGGTLRTSIEPGDITKGKLAELFPFGSVAQTVKLSGSDIVNALE
VSVGEYPTEQGGFLQVSGITFSFDPTKEKGNRVFDVKVGENPIDLNKEYTVAINDFLSQGGDGYTMLKTTVVGEFATYDD
IFAKYLNDNGMKNDINLGRIQVKENGKQVVTNQNNVVPVQAVPTVQEPQVSAKTETVYTVIAGDNLTKIARINNTTWQTL
AQYNNLQNPDLIYVGDQIKIPA
>Mature_582_residues
MKKKILSAMLSLTLVFSLNTTSVWAASKDDSTKITIIHTNDTHSRVLSADGGFGFAKIATIANETKKENPNTLLVDAGDT
LHGKPIVNVSKGENAVKILDAAGYDFMVPGNHDFNYGNERLIELSKLAKNFKMLDANVKKNGKDMLPPYEIIEKGNVKIG
IFGLSTPETAYKTNPANVKDVTFEDPIKVSEEMVKELKDKTDVIVALAHVGLDDSSVVTSKQIAEKVKGIDVIIDGHSHT
TLNDGLVVNNTLIAQTGEYDQNLGVVELEVKDKKVTEKKAKLLNSKDYKELKEDEKVTDLLNDIQAENDKVFSEVVASND
VLLDGVRGNVRTKETNLGNLTADATREVTKADIAFVNGGTLRTSIEPGDITKGKLAELFPFGSVAQTVKLSGSDIVNALE
VSVGEYPTEQGGFLQVSGITFSFDPTKEKGNRVFDVKVGENPIDLNKEYTVAINDFLSQGGDGYTMLKTTVVGEFATYDD
IFAKYLNDNGMKNDINLGRIQVKENGKQVVTNQNNVVPVQAVPTVQEPQVSAKTETVYTVIAGDNLTKIARINNTTWQTL
AQYNNLQNPDLIYVGDQIKIPA

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=523, Percent_Identity=30.9751434034417, Blast_Score=194, Evalue=3e-49,
Organism=Escherichia coli, GI1786687, Length=507, Percent_Identity=27.4161735700197, Blast_Score=179, Evalue=3e-46,
Organism=Escherichia coli, GI1790658, Length=552, Percent_Identity=26.0869565217391, Blast_Score=102, Evalue=5e-23,
Organism=Drosophila melanogaster, GI19921980, Length=451, Percent_Identity=29.7117516629712, Blast_Score=177, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24652512, Length=451, Percent_Identity=29.7117516629712, Blast_Score=177, Evalue=2e-44,
Organism=Drosophila melanogaster, GI161076508, Length=451, Percent_Identity=29.7117516629712, Blast_Score=177, Evalue=2e-44,
Organism=Drosophila melanogaster, GI28573524, Length=503, Percent_Identity=26.8389662027833, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI19922446, Length=536, Percent_Identity=27.6119402985075, Blast_Score=135, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24654424, Length=536, Percent_Identity=27.6119402985075, Blast_Score=135, Evalue=1e-31,
Organism=Drosophila melanogaster, GI19922444, Length=549, Percent_Identity=26.0473588342441, Blast_Score=132, Evalue=9e-31,
Organism=Drosophila melanogaster, GI221329836, Length=536, Percent_Identity=25.9328358208955, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24641187, Length=510, Percent_Identity=26.8627450980392, Blast_Score=122, Evalue=9e-28,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 63422; Mature: 63422

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00785 5_NUCLEOTIDASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKILSAMLSLTLVFSLNTTSVWAASKDDSTKITIIHTNDTHSRVLSADGGFGFAKIAT
CCHHHHHHHHHEEEEEEECCCEEEEECCCCCEEEEEEEECCCCCEEEECCCCCCHHHHHH
IANETKKENPNTLLVDAGDTLHGKPIVNVSKGENAVKILDAAGYDFMVPGNHDFNYGNER
HHHHHHCCCCCEEEEECCCCCCCCEEEEECCCCCEEEEEEECCCEEECCCCCCCCCCCHH
LIELSKLAKNFKMLDANVKKNGKDMLPPYEIIEKGNVKIGIFGLSTPETAYKTNPANVKD
HHHHHHHHHHHEEEECCCCCCCCCCCCCHHHHHCCCEEEEEEECCCCCCCCCCCCCCCEE
VTFEDPIKVSEEMVKELKDKTDVIVALAHVGLDDSSVVTSKQIAEKVKGIDVIIDGHSHT
CCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCEEEEEECCCCC
TLNDGLVVNNTLIAQTGEYDQNLGVVELEVKDKKVTEKKAKLLNSKDYKELKEDEKVTDL
EECCCEEEECEEEEECCCCCCCCCEEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHH
LNDIQAENDKVFSEVVASNDVLLDGVRGNVRTKETNLGNLTADATREVTKADIAFVNGGT
HHHHHCCCHHHHHHHHCCCCEEEECCCCCEEEECCCCCCCCCHHHHHHHHEEEEEEECCE
LRTSIEPGDITKGKLAELFPFGSVAQTVKLSGSDIVNALEVSVGEYPTEQGGFLQVSGIT
EEEECCCCCCCCCCHHHHCCCCCHHHEEEECCCHHHHHHHEECCCCCCCCCCEEEEECEE
FSFDPTKEKGNRVFDVKVGENPIDLNKEYTVAINDFLSQGGDGYTMLKTTVVGEFATYDD
EEECCCHHHCCEEEEEEECCCCCCCCCEEEEEEEHHHHCCCCCEEEEEEEEECCHHHHHH
IFAKYLNDNGMKNDINLGRIQVKENGKQVVTNQNNVVPVQAVPTVQEPQVSAKTETVYTV
HHHHHHCCCCCCCCCCEEEEEEECCCCEEEECCCCEEEEEECCCCCCCCCCCCCEEEEEE
IAGDNLTKIARINNTTWQTLAQYNNLQNPDLIYVGDQIKIPA
EECCCEEEEEEECCCHHHHHHHHCCCCCCCEEEECCEEECCC
>Mature Secondary Structure
MKKKILSAMLSLTLVFSLNTTSVWAASKDDSTKITIIHTNDTHSRVLSADGGFGFAKIAT
CCHHHHHHHHHEEEEEEECCCEEEEECCCCCEEEEEEEECCCCCEEEECCCCCCHHHHHH
IANETKKENPNTLLVDAGDTLHGKPIVNVSKGENAVKILDAAGYDFMVPGNHDFNYGNER
HHHHHHCCCCCEEEEECCCCCCCCEEEEECCCCCEEEEEEECCCEEECCCCCCCCCCCHH
LIELSKLAKNFKMLDANVKKNGKDMLPPYEIIEKGNVKIGIFGLSTPETAYKTNPANVKD
HHHHHHHHHHHEEEECCCCCCCCCCCCCHHHHHCCCEEEEEEECCCCCCCCCCCCCCCEE
VTFEDPIKVSEEMVKELKDKTDVIVALAHVGLDDSSVVTSKQIAEKVKGIDVIIDGHSHT
CCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCEEEEEECCCCC
TLNDGLVVNNTLIAQTGEYDQNLGVVELEVKDKKVTEKKAKLLNSKDYKELKEDEKVTDL
EECCCEEEECEEEEECCCCCCCCCEEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHH
LNDIQAENDKVFSEVVASNDVLLDGVRGNVRTKETNLGNLTADATREVTKADIAFVNGGT
HHHHHCCCHHHHHHHHCCCCEEEECCCCCEEEECCCCCCCCCHHHHHHHHEEEEEEECCE
LRTSIEPGDITKGKLAELFPFGSVAQTVKLSGSDIVNALEVSVGEYPTEQGGFLQVSGIT
EEEECCCCCCCCCCHHHHCCCCCHHHEEEECCCHHHHHHHEECCCCCCCCCCEEEEECEE
FSFDPTKEKGNRVFDVKVGENPIDLNKEYTVAINDFLSQGGDGYTMLKTTVVGEFATYDD
EEECCCHHHCCEEEEEEECCCCCCCCCEEEEEEEHHHHCCCCCEEEEEEEEECCHHHHHH
IFAKYLNDNGMKNDINLGRIQVKENGKQVVTNQNNVVPVQAVPTVQEPQVSAKTETVYTV
HHHHHHCCCCCCCCCCEEEEEEECCCCEEEECCCCEEEEEECCCCCCCCCCCCCEEEEEE
IAGDNLTKIARINNTTWQTLAQYNNLQNPDLIYVGDQIKIPA
EECCCEEEEEEECCCHHHHHHHHCCCCCCCEEEECCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]