| Definition | Clostridium botulinum B str. Eklund 17B, complete genome. |
|---|---|
| Accession | NC_010674 |
| Length | 3,800,327 |
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The map label for this gene is fruA [H]
Identifier: 187932403
GI number: 187932403
Start: 2365034
End: 2366926
Strand: Reverse
Name: fruA [H]
Synonym: CLL_A2296
Alternate gene names: 187932403
Gene position: 2366926-2365034 (Counterclockwise)
Preceding gene: 187932782
Following gene: 187935324
Centisome position: 62.28
GC content: 33.33
Gene sequence:
>1893_bases ATGAGAATTGTTGATTTATTAAAAAAACAAGGCATTGAGTTAAACTTTAACCCATCTTCTAAAGATGAATGTATTGAGAA ACTAGTAAACTTGATGGACAAAACAGGCAATTTAAATAACAAAGAAGAGTACAAAAAGGCTATTATAGCAAGGGAAGCAC AAAGTACAACTGGTATTGGTGAGGGAATAGCTATACCACACGGAAAAACCAATGCTGTTAAAAAAGCATCACTAGCTGCA GCAGTATGTAAAAAAGGGGTAGATTATGACTCGCTAGATGGAGCTCCAGCAAATTTATTTTTTATGATTGCAGTTCCAGA TAACAGTGATAACTTGCATTTAGAAGTTTTAGCTAGATTATCTACTATATTAATGGATGAAAAATTTAGAGAAACTTTAA TTAATTGTGAGGATAAAGACCAGTTTTTAAAGTTAATAGATGAAAAGGAAAGTGAAAAGTTTCCAGAAGAAGTTAAAGAA ACTAAACAAACATCAAGCAATGGTACATATAGAGTTTTAGCTGTTACAGCATGCCCAACAGGGATAGCTCATACTTATAT GGCAGCAGAAAGTCTTGAAAACAAGGGAAAAGACTTAGGTGTTACAATAAAAGTTGAAACTAATGGTTCTGGAGGATCTA AAAATGTTTTAACTAAAAAAGAAATTGAAGAAGCAGATTGTATTATAATTGCAGCAGATAAAAATGTTGAAATGAGTAGA TTTAATGGAAAAAGAGTTATAAAAACTAAAGTAGCAAATGGAATACATAAAGCAGAAGAGTTAATCAATGAAGCTATAAG TGGAAATGCACCTGTATATCATCATAATGGTAATGGCAGTGTTGAAACAGAGAATATAGAAAATGAAAGTATAGGTCGTC AAATTTATAAACATCTTATGAATGGAGTATCACATATGTTACCATTTGTAATTGGTGGAGGTATATTAATTGCATTAGCA TTCCTTTTTGATGATTATAGTATTGATCCAAGCAACTTTGGTATGAATACACCACTTGCAGCATTTTTTAAAACAGTAGG TGGAACAGCTTTTGACTTTATGCTTCCAGTGCTTGCAGGATTTATTGCAATGAGTATTGGAGATAGGCCAGCACTAGCAG TAGGGTTCGTTGGTGGAGCATTAGCTAATAAAGGTGGATCAGGATTCTTAGGTGCATTACTTGCAGGATTTATAGCTGGT TACTTAGTTGTTTTATTAAAGAAATTATTCGATAAATTACCTCAGAGCTTAGAAGGTTTAAAACCTGTATTATTATATCC TTTCTTTGGTATATTACTAATTGGAGCAATTATGGTGTTTGCTGTTAATCCACCGGTTGGTGCTTTAAATACTATGATTA CAAATGGACTTAATTCAATGGGGGGAACTAGTAAAGTTCTTTTAGGTATAGTATTAGGTGGAATGATGTCAGTTGATATG GGAGGTCCTGTAAACAAAGCAGCATATGTCTTCGGAACAGCATCACTTGCAAGTGGCAATTTTGAAATAATGGCAGCTGT AATGGCTGGTGGTATGGTTCCACCACTTGCAATAGCATTAAGTACTACTTTCTTTAAAAATAGATATACAGTAAGGGAAA GACAATCAGGTCTTACTAACTATATAATGGGTTTATCTTTTATAACAGAAGGAGCAATTCCATTTGCAGCAGCAGATCCA TTAAGAGTAATACCAGCTTGTGTAGTTGGTTCAGCAGTTGCTGGTGGATTATCAATGTTCTTTGAGTGTGCTTTAAGAGC TCCACATGGTGGATTATTTGTAATAGGTATTATAAGTAATAAATTGGGTTATATTGGAGCAATAGCAGCGGGTGCAGTAG TAGGTATGATAATACTTTCAGTATTAAAAAAACCAATAAAAGATAATAAATAA
Upstream 100 bases:
>100_bases TGCAACAGGTAGTGCAAGTGCATTTTCTGATGAATTAGCAACAAAAGAAGAAGTTGAAAAACTATTATCTAAAATGTAAA AATGCAAGGAGGAGTATTTA
Downstream 100 bases:
>100_bases TATTATAAAGAAATCCCCTTTAGAGCGAATGATTATTTACTCTAAAGGGGATTTTTATGTATAAAAATATTGCAATTTCA CCTAAAGTATGTTATACTGA
Product: PTS system, fructose-specific component family
Products: NA
Alternate protein names: EIIABC-Fru; Fructose-specific phosphotransferase enzyme IIA component; EII-Fru; PTS system fructose-specific EIIA component; Fructose-specific phosphotransferase enzyme IIB component; EIII-Fru; PTS system fructose-specific EIIB component; Fructose permease IIC component; PTS system fructose-specific EIIC component [H]
Number of amino acids: Translated: 630; Mature: 630
Protein sequence:
>630_residues MRIVDLLKKQGIELNFNPSSKDECIEKLVNLMDKTGNLNNKEEYKKAIIAREAQSTTGIGEGIAIPHGKTNAVKKASLAA AVCKKGVDYDSLDGAPANLFFMIAVPDNSDNLHLEVLARLSTILMDEKFRETLINCEDKDQFLKLIDEKESEKFPEEVKE TKQTSSNGTYRVLAVTACPTGIAHTYMAAESLENKGKDLGVTIKVETNGSGGSKNVLTKKEIEEADCIIIAADKNVEMSR FNGKRVIKTKVANGIHKAEELINEAISGNAPVYHHNGNGSVETENIENESIGRQIYKHLMNGVSHMLPFVIGGGILIALA FLFDDYSIDPSNFGMNTPLAAFFKTVGGTAFDFMLPVLAGFIAMSIGDRPALAVGFVGGALANKGGSGFLGALLAGFIAG YLVVLLKKLFDKLPQSLEGLKPVLLYPFFGILLIGAIMVFAVNPPVGALNTMITNGLNSMGGTSKVLLGIVLGGMMSVDM GGPVNKAAYVFGTASLASGNFEIMAAVMAGGMVPPLAIALSTTFFKNRYTVRERQSGLTNYIMGLSFITEGAIPFAAADP LRVIPACVVGSAVAGGLSMFFECALRAPHGGLFVIGIISNKLGYIGAIAAGAVVGMIILSVLKKPIKDNK
Sequences:
>Translated_630_residues MRIVDLLKKQGIELNFNPSSKDECIEKLVNLMDKTGNLNNKEEYKKAIIAREAQSTTGIGEGIAIPHGKTNAVKKASLAA AVCKKGVDYDSLDGAPANLFFMIAVPDNSDNLHLEVLARLSTILMDEKFRETLINCEDKDQFLKLIDEKESEKFPEEVKE TKQTSSNGTYRVLAVTACPTGIAHTYMAAESLENKGKDLGVTIKVETNGSGGSKNVLTKKEIEEADCIIIAADKNVEMSR FNGKRVIKTKVANGIHKAEELINEAISGNAPVYHHNGNGSVETENIENESIGRQIYKHLMNGVSHMLPFVIGGGILIALA FLFDDYSIDPSNFGMNTPLAAFFKTVGGTAFDFMLPVLAGFIAMSIGDRPALAVGFVGGALANKGGSGFLGALLAGFIAG YLVVLLKKLFDKLPQSLEGLKPVLLYPFFGILLIGAIMVFAVNPPVGALNTMITNGLNSMGGTSKVLLGIVLGGMMSVDM GGPVNKAAYVFGTASLASGNFEIMAAVMAGGMVPPLAIALSTTFFKNRYTVRERQSGLTNYIMGLSFITEGAIPFAAADP LRVIPACVVGSAVAGGLSMFFECALRAPHGGLFVIGIISNKLGYIGAIAAGAVVGMIILSVLKKPIKDNK >Mature_630_residues MRIVDLLKKQGIELNFNPSSKDECIEKLVNLMDKTGNLNNKEEYKKAIIAREAQSTTGIGEGIAIPHGKTNAVKKASLAA AVCKKGVDYDSLDGAPANLFFMIAVPDNSDNLHLEVLARLSTILMDEKFRETLINCEDKDQFLKLIDEKESEKFPEEVKE TKQTSSNGTYRVLAVTACPTGIAHTYMAAESLENKGKDLGVTIKVETNGSGGSKNVLTKKEIEEADCIIIAADKNVEMSR FNGKRVIKTKVANGIHKAEELINEAISGNAPVYHHNGNGSVETENIENESIGRQIYKHLMNGVSHMLPFVIGGGILIALA FLFDDYSIDPSNFGMNTPLAAFFKTVGGTAFDFMLPVLAGFIAMSIGDRPALAVGFVGGALANKGGSGFLGALLAGFIAG YLVVLLKKLFDKLPQSLEGLKPVLLYPFFGILLIGAIMVFAVNPPVGALNTMITNGLNSMGGTSKVLLGIVLGGMMSVDM GGPVNKAAYVFGTASLASGNFEIMAAVMAGGMVPPLAIALSTTFFKNRYTVRERQSGLTNYIMGLSFITEGAIPFAAADP LRVIPACVVGSAVAGGLSMFFECALRAPHGGLFVIGIISNKLGYIGAIAAGAVVGMIILSVLKKPIKDNK
Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This
COG id: COG1299
COG function: function code G; Phosphotransferase system, fructose-specific IIC component
Gene ontology:
Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 PTS EIIC type-2 domain [H]
Homologues:
Organism=Escherichia coli, GI1788492, Length=466, Percent_Identity=45.2789699570815, Blast_Score=402, Evalue=1e-113, Organism=Escherichia coli, GI1786951, Length=642, Percent_Identity=33.8006230529595, Blast_Score=353, Evalue=1e-98, Organism=Escherichia coli, GI87082348, Length=468, Percent_Identity=32.6923076923077, Blast_Score=246, Evalue=5e-66, Organism=Escherichia coli, GI1790386, Length=325, Percent_Identity=38.7692307692308, Blast_Score=211, Evalue=1e-55, Organism=Escherichia coli, GI1788729, Length=384, Percent_Identity=25.2604166666667, Blast_Score=115, Evalue=1e-26, Organism=Escherichia coli, GI1790387, Length=95, Percent_Identity=40, Blast_Score=85, Evalue=2e-17, Organism=Escherichia coli, GI2367327, Length=140, Percent_Identity=24.2857142857143, Blast_Score=66, Evalue=6e-12, Organism=Escherichia coli, GI1788730, Length=95, Percent_Identity=36.8421052631579, Blast_Score=66, Evalue=7e-12, Organism=Escherichia coli, GI1788726, Length=142, Percent_Identity=27.4647887323944, Blast_Score=62, Evalue=9e-11,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR016152 - InterPro: IPR002178 - InterPro: IPR013011 - InterPro: IPR003501 - InterPro: IPR003352 - InterPro: IPR013014 - InterPro: IPR004715 - InterPro: IPR003353 - InterPro: IPR006327 [H]
Pfam domain/function: PF00359 PTS_EIIA_2; PF02378 PTS_EIIC; PF02302 PTS_IIB [H]
EC number: =2.7.1.69 [H]
Molecular weight: Translated: 66846; Mature: 66846
Theoretical pI: Translated: 7.52; Mature: 7.52
Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2 ; PS51099 PTS_EIIB_TYPE_2 ; PS51104 PTS_EIIC_TYPE_2
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.1 %Cys (Translated Protein) 3.7 %Met (Translated Protein) 4.8 %Cys+Met (Translated Protein) 1.1 %Cys (Mature Protein) 3.7 %Met (Mature Protein) 4.8 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MRIVDLLKKQGIELNFNPSSKDECIEKLVNLMDKTGNLNNKEEYKKAIIAREAQSTTGIG CCHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCC EGIAIPHGKTNAVKKASLAAAVCKKGVDYDSLDGAPANLFFMIAVPDNSDNLHLEVLARL CCEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHH STILMDEKFRETLINCEDKDQFLKLIDEKESEKFPEEVKETKQTSSNGTYRVLAVTACPT HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEEEEECCC GIAHTYMAAESLENKGKDLGVTIKVETNGSGGSKNVLTKKEIEEADCIIIAADKNVEMSR HHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCCHHHCCCCCEEEEEECCCCCHHH FNGKRVIKTKVANGIHKAEELINEAISGNAPVYHHNGNGSVETENIENESIGRQIYKHLM CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEECCCCCHHHHHHHHHHHHH NGVSHMLPFVIGGGILIALAFLFDDYSIDPSNFGMNTPLAAFFKTVGGTAFDFMLPVLAG HHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHH FIAMSIGDRPALAVGFVGGALANKGGSGFLGALLAGFIAGYLVVLLKKLFDKLPQSLEGL HHHHHCCCCCCEEEEHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC KPVLLYPFFGILLIGAIMVFAVNPPVGALNTMITNGLNSMGGTSKVLLGIVLGGMMSVDM CHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHEECC GGPVNKAAYVFGTASLASGNFEIMAAVMAGGMVPPLAIALSTTFFKNRYTVRERQSGLTN CCCCCCCEEEEEEHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHCCHHH YIMGLSFITEGAIPFAAADPLRVIPACVVGSAVAGGLSMFFECALRAPHGGLFVIGIISN HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECC KLGYIGAIAAGAVVGMIILSVLKKPIKDNK CCHHHHHHHHHHHHHHHHHHHHHHHCCCCC >Mature Secondary Structure MRIVDLLKKQGIELNFNPSSKDECIEKLVNLMDKTGNLNNKEEYKKAIIAREAQSTTGIG CCHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCC EGIAIPHGKTNAVKKASLAAAVCKKGVDYDSLDGAPANLFFMIAVPDNSDNLHLEVLARL CCEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHH STILMDEKFRETLINCEDKDQFLKLIDEKESEKFPEEVKETKQTSSNGTYRVLAVTACPT HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEEEEECCC GIAHTYMAAESLENKGKDLGVTIKVETNGSGGSKNVLTKKEIEEADCIIIAADKNVEMSR HHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCCHHHCCCCCEEEEEECCCCCHHH FNGKRVIKTKVANGIHKAEELINEAISGNAPVYHHNGNGSVETENIENESIGRQIYKHLM CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEECCCCCHHHHHHHHHHHHH NGVSHMLPFVIGGGILIALAFLFDDYSIDPSNFGMNTPLAAFFKTVGGTAFDFMLPVLAG HHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHH FIAMSIGDRPALAVGFVGGALANKGGSGFLGALLAGFIAGYLVVLLKKLFDKLPQSLEGL HHHHHCCCCCCEEEEHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC KPVLLYPFFGILLIGAIMVFAVNPPVGALNTMITNGLNSMGGTSKVLLGIVLGGMMSVDM CHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHEECC GGPVNKAAYVFGTASLASGNFEIMAAVMAGGMVPPLAIALSTTFFKNRYTVRERQSGLTN CCCCCCCEEEEEEHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHCCHHH YIMGLSFITEGAIPFAAADPLRVIPACVVGSAVAGGLSMFFECALRAPHGGLFVIGIISN HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECC KLGYIGAIAAGAVVGMIILSVLKKPIKDNK CCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 6.0
TargetDB status: NA
Availability: NA
References: 9384377 [H]