Click here to switch to the map view.

The map label for this gene is truB [H]

Identifier: 187918657

GI number: 187918657

Start: 861050

End: 861895

Strand: Direct

Name: truB [H]

Synonym: BH0803

Alternate gene names: 187918657

Gene position: 861050-861895 (Clockwise)

Preceding gene: 187918656

Following gene: 187918658

Centisome position: 93.36

GC content: 29.67

Gene sequence:

>846_bases
GTGAATGGGATTATTTTATTAAATAAGAGAATTGGAATAACTTCTTGTGATACCCTTTTTCCTTTAAAGAGGTATTTTTC
TACAAGTCGAGTTGGACATACGGGTACTCTTGATAAGTTTGCAAGTGGTCTTTTGGTTGTTTTAGTTGGCAAATACACCA
AACTTTCAAATTACATTACATCTTTAGATAAAGAATATATATCAGAGTTTGAGTTTGGAATTGAAACTGATACCCTTGAT
CCTAACGGTAGAATAGCAAATAGGACAGATTATATTCCAAATTTGGAAGAATTAAGTCTTGGGATTAGGTCTTTTATAGG
TGAGATTTATCAAATTCCACCCAAGTTTTCTTCAGTGCATGTTAAAGGAAAAAGAGCTTATAAGTTAGCTTTAAGTGGGG
AGTATTTTGAGCTTCAGCCTAGAAAAGTCAATATATATGATATTCAGATCTTAAATTATAATGTTGATTCTCGTATTTTG
AAATTACAAATAAAATGCTCTAAGGGAACTTATGTTAGAAGTATAGCAAGAGATTTGGCATTGTCTTTGGGGTCATTTGC
TTATGTTAAGAGCCTTGAGAGAATTAAGATTGGAGATTTTAGATTAGATAATGCTTGTTTTTGTGAAGATTTCAATAGCA
ATTCTTTAATGAGTTTAGAATCTTTGGGGCTTTTTGAAAAAATTTATGTTGATAATAGTATGATTAAGCTTATTCGGAAT
GGTGCTTATATCAATGTTGTTATTAATGTTGGTGAGTTTAAGATTTTAAAATCTTACAATGAAGAGATATTAGCAATAAT
TTGTGGCATTGGCTTGAATAAATATAAGTATATTATTATTTTTTGA

Upstream 100 bases:

>100_bases
TCCAAAATTAAGTTTTTTAAGAGATGATACTATTTCTAAGGCTTTTTATGTTAATAAGATAATTGAAAATTTAAGTTTTA
GTGACGAATAGTAAAATTTA

Downstream 100 bases:

>100_bases
TATTTAATTTCTGAAAACTAATTAGTAGTATAAATATTTTAAGAGCAAGATTATTAGTCTTTATCGCGGTGCCCTTTTAC
AAGTAATAGTAAGATAGAAA

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MNGIILLNKRIGITSCDTLFPLKRYFSTSRVGHTGTLDKFASGLLVVLVGKYTKLSNYITSLDKEYISEFEFGIETDTLD
PNGRIANRTDYIPNLEELSLGIRSFIGEIYQIPPKFSSVHVKGKRAYKLALSGEYFELQPRKVNIYDIQILNYNVDSRIL
KLQIKCSKGTYVRSIARDLALSLGSFAYVKSLERIKIGDFRLDNACFCEDFNSNSLMSLESLGLFEKIYVDNSMIKLIRN
GAYINVVINVGEFKILKSYNEEILAIICGIGLNKYKYIIIF

Sequences:

>Translated_281_residues
MNGIILLNKRIGITSCDTLFPLKRYFSTSRVGHTGTLDKFASGLLVVLVGKYTKLSNYITSLDKEYISEFEFGIETDTLD
PNGRIANRTDYIPNLEELSLGIRSFIGEIYQIPPKFSSVHVKGKRAYKLALSGEYFELQPRKVNIYDIQILNYNVDSRIL
KLQIKCSKGTYVRSIARDLALSLGSFAYVKSLERIKIGDFRLDNACFCEDFNSNSLMSLESLGLFEKIYVDNSMIKLIRN
GAYINVVINVGEFKILKSYNEEILAIICGIGLNKYKYIIIF
>Mature_281_residues
MNGIILLNKRIGITSCDTLFPLKRYFSTSRVGHTGTLDKFASGLLVVLVGKYTKLSNYITSLDKEYISEFEFGIETDTLD
PNGRIANRTDYIPNLEELSLGIRSFIGEIYQIPPKFSSVHVKGKRAYKLALSGEYFELQPRKVNIYDIQILNYNVDSRIL
KLQIKCSKGTYVRSIARDLALSLGSFAYVKSLERIKIGDFRLDNACFCEDFNSNSLMSLESLGLFEKIYVDNSMIKLIRN
GAYINVVINVGEFKILKSYNEEILAIICGIGLNKYKYIIIF

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=228, Percent_Identity=33.7719298245614, Blast_Score=112, Evalue=3e-25,
Organism=Escherichia coli, GI2367200, Length=209, Percent_Identity=34.4497607655502, Blast_Score=135, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6324037, Length=134, Percent_Identity=35.0746268656716, Blast_Score=80, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6323204, Length=197, Percent_Identity=23.8578680203046, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI281364189, Length=238, Percent_Identity=23.5294117647059, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI281364187, Length=238, Percent_Identity=23.5294117647059, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI281364185, Length=238, Percent_Identity=23.5294117647059, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI281364183, Length=238, Percent_Identity=23.5294117647059, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI62471759, Length=238, Percent_Identity=23.5294117647059, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI17975520, Length=238, Percent_Identity=23.5294117647059, Blast_Score=68, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780 [H]

Pfam domain/function: PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 31925; Mature: 31925

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGIILLNKRIGITSCDTLFPLKRYFSTSRVGHTGTLDKFASGLLVVLVGKYTKLSNYIT
CCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHCCEEEEEECCHHHHHHHHH
SLDKEYISEFEFGIETDTLDPNGRIANRTDYIPNLEELSLGIRSFIGEIYQIPPKFSSVH
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEE
VKGKRAYKLALSGEYFELQPRKVNIYDIQILNYNVDSRILKLQIKCSKGTYVRSIARDLA
EECCEEEEEEECCCEEEECCCEEEEEEEEEEEECCCCEEEEEEEEECCCHHHHHHHHHHH
LSLGSFAYVKSLERIKIGDFRLDNACFCEDFNSNSLMSLESLGLFEKIYVDNSMIKLIRN
HHHCCHHHHHHHHHEEECCEEECCEEEEECCCCCCEEEHHHCCCEEHHHHCHHHHHHHHC
GAYINVVINVGEFKILKSYNEEILAIICGIGLNKYKYIIIF
CCEEEEEEECCCEEEEHHCCHHEEEEEECCCCCCEEEEEEC
>Mature Secondary Structure
MNGIILLNKRIGITSCDTLFPLKRYFSTSRVGHTGTLDKFASGLLVVLVGKYTKLSNYIT
CCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHCCEEEEEECCHHHHHHHHH
SLDKEYISEFEFGIETDTLDPNGRIANRTDYIPNLEELSLGIRSFIGEIYQIPPKFSSVH
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEE
VKGKRAYKLALSGEYFELQPRKVNIYDIQILNYNVDSRILKLQIKCSKGTYVRSIARDLA
EECCEEEEEEECCCEEEECCCEEEEEEEEEEEECCCCEEEEEEEEECCCHHHHHHHHHHH
LSLGSFAYVKSLERIKIGDFRLDNACFCEDFNSNSLMSLESLGLFEKIYVDNSMIKLIRN
HHHCCHHHHHHHHHEEECCEEECCEEEEECCCCCCEEEHHHCCCEEHHHHCHHHHHHHHC
GAYINVVINVGEFKILKSYNEEILAIICGIGLNKYKYIIIF
CCEEEEEEECCCEEEEHHCCHHEEEEEECCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA