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The map label for this gene is nusA [H]

Identifier: 187918654

GI number: 187918654

Start: 856568

End: 858058

Strand: Direct

Name: nusA [H]

Synonym: BH0800

Alternate gene names: 187918654

Gene position: 856568-858058 (Clockwise)

Preceding gene: 187918653

Following gene: 187918655

Centisome position: 92.87

GC content: 33.2

Gene sequence:

>1491_bases
GTGATGTCAAGAAGGCTAAATTATCGTGAAGGAGTCTTGGATATGATAAAGGGTACTGGTCAAATGATTTCCAATATTGC
TAGTGAGCGAGGAATGAGCATAGATGCTATTCGAAAGACGGTTAGGGAATCTGTAATGATAGCTTATAAGAAGTATTTTG
GAACAAGTGAGAATGCTTTAATTAAATTTGATGAAGATACTGGAGATTTAGTAGTTTATTCTAAAAAAAAGATCGTAGAG
GAAGTTCAAAACGATATACTTGAGATATTGCAAGATGATATTCAAGAATTTGAAGTCGTGGAAGATGGATATGCATATAT
TGAGATTGATCCCAAGATTTTTGATAGACTTTCAATTCAAGTCGCTAAACAGAGAACTAAGAGCGATTTGCAGGGAATCG
AAGATAATGAGCTTTACTTAGAATTTAAACACAAATTGCATAAGATTGTTATTGGTTATGTTCAACAGAATAGAAATGGA
GATCTTTATGTTAATCTTGGCAGTACAGATGGTGTTATCCCTAAAAAGTATCAATCTTCAAGGGAAGTTTATGGACTTAA
TGATAAAGTTCGAGTTCTTGTTTACAGTGTAAAGAAGGGTAAAAATGGAATAGAGGTAGTCTTGTCAAGAACTCACCCTA
AATTTATTGAAGAACTTCTTACTCTTGAGATTCCTGAAATTGAAGAAGGTATTATTAAAATCCATAAGATAGTAAGAGAT
CCAGGTTATAGAACTAAGGTTGCTGTTTATTCTGAGAAAGAAGAGATTGATCCTGTAGGTCCTTGTATTGGGCAAAAAGG
TGTTAGAATTCAATCAATAATTAAGGAACTTGAAGGTGAGAAAATAGATATTATTCCTTATTCTAGGGATATTAAAGAAT
TTATTAGAGATGCCTTAACTCCTGCTAAGATAGATAATGTATATATTGTTGATGAGGATTTACATAAAGCTTTAGTGGTT
GTTAGTGATGAACAGCTATCGCTTGCAATAGGTAAGATGGGACAAAATGTTAGACTTGCAAATAGACTTCTTGATTGGGC
AATTGATGTTAAAACTAATAGTCAGTTTGCGGAAATGAAAGCAAGTGGAGAGTTTAAACAAGAGACTTTTGAAATGTTTG
ACAAGATTCTTCAAGATAATGTTCAGGAAGATGAATTCGAGGAGATAAACAGGATTAGTGAGCTTAAGATTCTTGATAGT
GATATTGTTGACAAGTTGATTGAAGCAGGTCTTGATGATATTGATAATTTTTTGGATGCTAGCGAGGAAAACCTTTTTGA
GTTAGGAATAAGCTATGAAAAGCAAGAAGAAATCAATAAGATATTGAAGGAAGGGATGGTAATAATTGCTAATGATGATG
ATGGATCTATTGAAGGCATAAAAGATGAAGAAGAGTTGCTTTGTCCTGAGTGTGGAGCTGTTATTAATGAAAATATGACA
TTTTGTCCAGGTTGTAAGATAGGGCTTAGCTTTGAATTTGAAGAGGAGTAA

Upstream 100 bases:

>100_bases
TTAATGCTAGATAATGATTTTGAAGAAGGTTTTATTTTAAAAGCAGGAGCAGATAGTTTTATTTTTAAAACAGATAATAA
AGAGGTAAAAGTTCTTTATA

Downstream 100 bases:

>100_bases
TAGTTTGTCAGAAAATATTGATGATGATCGCAGTGAAGATGAAAAAAAAATTAAGGTTGTTAAATTACGAAAGAAAGTGG
TAAAGGTTGTAGCCCATACT

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 496; Mature: 496

Protein sequence:

>496_residues
MMSRRLNYREGVLDMIKGTGQMISNIASERGMSIDAIRKTVRESVMIAYKKYFGTSENALIKFDEDTGDLVVYSKKKIVE
EVQNDILEILQDDIQEFEVVEDGYAYIEIDPKIFDRLSIQVAKQRTKSDLQGIEDNELYLEFKHKLHKIVIGYVQQNRNG
DLYVNLGSTDGVIPKKYQSSREVYGLNDKVRVLVYSVKKGKNGIEVVLSRTHPKFIEELLTLEIPEIEEGIIKIHKIVRD
PGYRTKVAVYSEKEEIDPVGPCIGQKGVRIQSIIKELEGEKIDIIPYSRDIKEFIRDALTPAKIDNVYIVDEDLHKALVV
VSDEQLSLAIGKMGQNVRLANRLLDWAIDVKTNSQFAEMKASGEFKQETFEMFDKILQDNVQEDEFEEINRISELKILDS
DIVDKLIEAGLDDIDNFLDASEENLFELGISYEKQEEINKILKEGMVIIANDDDGSIEGIKDEEELLCPECGAVINENMT
FCPGCKIGLSFEFEEE

Sequences:

>Translated_496_residues
MMSRRLNYREGVLDMIKGTGQMISNIASERGMSIDAIRKTVRESVMIAYKKYFGTSENALIKFDEDTGDLVVYSKKKIVE
EVQNDILEILQDDIQEFEVVEDGYAYIEIDPKIFDRLSIQVAKQRTKSDLQGIEDNELYLEFKHKLHKIVIGYVQQNRNG
DLYVNLGSTDGVIPKKYQSSREVYGLNDKVRVLVYSVKKGKNGIEVVLSRTHPKFIEELLTLEIPEIEEGIIKIHKIVRD
PGYRTKVAVYSEKEEIDPVGPCIGQKGVRIQSIIKELEGEKIDIIPYSRDIKEFIRDALTPAKIDNVYIVDEDLHKALVV
VSDEQLSLAIGKMGQNVRLANRLLDWAIDVKTNSQFAEMKASGEFKQETFEMFDKILQDNVQEDEFEEINRISELKILDS
DIVDKLIEAGLDDIDNFLDASEENLFELGISYEKQEEINKILKEGMVIIANDDDGSIEGIKDEEELLCPECGAVINENMT
FCPGCKIGLSFEFEEE
>Mature_496_residues
MMSRRLNYREGVLDMIKGTGQMISNIASERGMSIDAIRKTVRESVMIAYKKYFGTSENALIKFDEDTGDLVVYSKKKIVE
EVQNDILEILQDDIQEFEVVEDGYAYIEIDPKIFDRLSIQVAKQRTKSDLQGIEDNELYLEFKHKLHKIVIGYVQQNRNG
DLYVNLGSTDGVIPKKYQSSREVYGLNDKVRVLVYSVKKGKNGIEVVLSRTHPKFIEELLTLEIPEIEEGIIKIHKIVRD
PGYRTKVAVYSEKEEIDPVGPCIGQKGVRIQSIIKELEGEKIDIIPYSRDIKEFIRDALTPAKIDNVYIVDEDLHKALVV
VSDEQLSLAIGKMGQNVRLANRLLDWAIDVKTNSQFAEMKASGEFKQETFEMFDKILQDNVQEDEFEEINRISELKILDS
DIVDKLIEAGLDDIDNFLDASEENLFELGISYEKQEEINKILKEGMVIIANDDDGSIEGIKDEEELLCPECGAVINENMT
FCPGCKIGLSFEFEEE

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=489, Percent_Identity=27.8118609406953, Blast_Score=185, Evalue=7e-48,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 56596; Mature: 56596

Theoretical pI: Translated: 4.35; Mature: 4.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSRRLNYREGVLDMIKGTGQMISNIASERGMSIDAIRKTVRESVMIAYKKYFGTSENAL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCE
IKFDEDTGDLVVYSKKKIVEEVQNDILEILQDDIQEFEVVEDGYAYIEIDPKIFDRLSIQ
EEEECCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHH
VAKQRTKSDLQGIEDNELYLEFKHKLHKIVIGYVQQNRNGDLYVNLGSTDGVIPKKYQSS
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHEECCCCCEEEEECCCCCCCCCHHCCC
REVYGLNDKVRVLVYSVKKGKNGIEVVLSRTHPKFIEELLTLEIPEIEEGIIKIHKIVRD
CCEECCCCCEEEEEEEECCCCCCEEEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHC
PGYRTKVAVYSEKEEIDPVGPCIGQKGVRIQSIIKELEGEKIDIIPYSRDIKEFIRDALT
CCCCEEEEEECCHHCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHCCC
PAKIDNVYIVDEDLHKALVVVSDEQLSLAIGKMGQNVRLANRLLDWAIDVKTNSQFAEMK
CCCCCCEEEECCCCCCEEEEEECCHHHHHHHHCCCCHHHHHHHHHHEEEECCCCHHHHHH
ASGEFKQETFEMFDKILQDNVQEDEFEEINRISELKILDSDIVDKLIEAGLDDIDNFLDA
CCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCC
SEENLFELGISYEKQEEINKILKEGMVIIANDDDGSIEGIKDEEELLCPECGAVINENMT
CCCCHHHHCCCCCHHHHHHHHHHCCEEEEEECCCCCCCCCCCHHHHCCCHHHHHHCCCCE
FCPGCKIGLSFEFEEE
ECCCCCCCEEEEECCC
>Mature Secondary Structure
MMSRRLNYREGVLDMIKGTGQMISNIASERGMSIDAIRKTVRESVMIAYKKYFGTSENAL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCE
IKFDEDTGDLVVYSKKKIVEEVQNDILEILQDDIQEFEVVEDGYAYIEIDPKIFDRLSIQ
EEEECCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHH
VAKQRTKSDLQGIEDNELYLEFKHKLHKIVIGYVQQNRNGDLYVNLGSTDGVIPKKYQSS
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHEECCCCCEEEEECCCCCCCCCHHCCC
REVYGLNDKVRVLVYSVKKGKNGIEVVLSRTHPKFIEELLTLEIPEIEEGIIKIHKIVRD
CCEECCCCCEEEEEEEECCCCCCEEEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHC
PGYRTKVAVYSEKEEIDPVGPCIGQKGVRIQSIIKELEGEKIDIIPYSRDIKEFIRDALT
CCCCEEEEEECCHHCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHCCC
PAKIDNVYIVDEDLHKALVVVSDEQLSLAIGKMGQNVRLANRLLDWAIDVKTNSQFAEMK
CCCCCCEEEECCCCCCEEEEEECCHHHHHHHHCCCCHHHHHHHHHHEEEECCCCHHHHHH
ASGEFKQETFEMFDKILQDNVQEDEFEEINRISELKILDSDIVDKLIEAGLDDIDNFLDA
CCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCC
SEENLFELGISYEKQEEINKILKEGMVIIANDDDGSIEGIKDEEELLCPECGAVINENMT
CCCCHHHHCCCCCHHHHHHHHHHCCEEEEEECCCCCCCCCCCHHHHCCCHHHHHHCCCCE
FCPGCKIGLSFEFEEE
ECCCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685 [H]