Click here to switch to the map view.

The map label for this gene is pflA

Identifier: 187734201

GI number: 187734201

Start: 2225352

End: 2226119

Strand: Direct

Name: pflA

Synonym: SbBS512_E2426

Alternate gene names: 187734201

Gene position: 2225352-2226119 (Clockwise)

Preceding gene: 187733224

Following gene: 187732506

Centisome position: 48.21

GC content: 50.65

Gene sequence:

>768_bases
ATGGGCCACATCTGGAGAAACACCGCAATGTCAGTTATTGGTCGCATTCACTCCTTTGAATCCTGTGGAACCGTAGACGG
CCCGGGTATTCGCTTTATCACCTTTTTCCAGGGCTGCCTGATGCGCTGCCTGTATTGTCATAACCGCGACACCTGGGACA
CGCATGGCGGTAAAGAAGTTACCGTTGAAGATTTGATGAAGGAAGTGGTGACCTATCGCCACTTTATGAACGCTTCCGGC
GGCGGCGTTACCGCATCCGGCGGTGAAGCAATCCTGCAAGCTGAGTTTGTTCGTGACTGGTTCCGCGCCTGCAAAAAAGA
AGGCATTCATACCTGTCTGGACACCAACGGTTTTGTTCGTCGTTACGATCCGGTGATTGATGAACTGCTGGAAGTAACCG
ACCTGGTAATGCTCGATCTCAAACAGATGAACGACGAGATCCACCAAAATCTGGTTGGAGTTTCCAACCACCGCACGCTG
GAGTTCGCCAAATATCTGGCGAACAAAAATGTGAAGGTGTGGATCCGCTACGTTGTTGTCCCAGGCTGGTCTGACGATGA
CGATTCAGCGCATCGCCTCGGTGAATTTACCCGTGATATGGGCAACGTTGAGAAAATCGAACTCCTCCCCTACCACGAGC
TGGGCAAACACAAATGGGTGGCAATGGGTGAAGAGTACAAACTCGACGGTGTTAAACCACCTAAGAAAGAGACCATGGAA
CGCGTGAAAGGCATTCTTGAGCAGTACGGTCATAAGGTAATGTTCTAA

Upstream 100 bases:

>100_bases
TTTTAGCACGAGAGCCTTTTTTGTCAGCTATCTATACTTTAAGGTGACTGCCAAAACAGACTCGACGTAGCCTTCGAGCT
GCGCACCAACACGGCCTCAG

Downstream 100 bases:

>100_bases
TTTCCCCGTAAAGCGGCAACTTTATTGAGTTGCCGCTTTTTTATTCCGCGCTTCATTCTGCTACAGTGTTTTTAACACAT
TTTATAGTGCATCTACCATA

Product: pyruvate formate lyase-activating enzyme 1

Products: NA

Alternate protein names: Formate-C-acetyltransferase-activating enzyme 1; PFL-activating enzyme 1 [H]

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MGHIWRNTAMSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWDTHGGKEVTVEDLMKEVVTYRHFMNASG
GGVTASGGEAILQAEFVRDWFRACKKEGIHTCLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTL
EFAKYLANKNVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYKLDGVKPPKKETME
RVKGILEQYGHKVMF

Sequences:

>Translated_255_residues
MGHIWRNTAMSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWDTHGGKEVTVEDLMKEVVTYRHFMNASG
GGVTASGGEAILQAEFVRDWFRACKKEGIHTCLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTL
EFAKYLANKNVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYKLDGVKPPKKETME
RVKGILEQYGHKVMF
>Mature_254_residues
GHIWRNTAMSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWDTHGGKEVTVEDLMKEVVTYRHFMNASGG
GVTASGGEAILQAEFVRDWFRACKKEGIHTCLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLE
FAKYLANKNVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYKLDGVKPPKKETMER
VKGILEQYGHKVMF

Specific function: Activation of pyruvate formate-lyase 1 under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family [H]

Homologues:

Organism=Escherichia coli, GI1787130, Length=246, Percent_Identity=100, Blast_Score=512, Evalue=1e-147,
Organism=Escherichia coli, GI1790389, Length=274, Percent_Identity=25.9124087591241, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1790839, Length=291, Percent_Identity=27.1477663230241, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI226510931, Length=174, Percent_Identity=32.7586206896552, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR012838
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 29272; Mature: 29141

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGHIWRNTAMSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWDTHGGKEV
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEE
TVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHTCLDTNGFVR
EHHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHH
RYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANKNVKVWIRYVVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEEEEEE
PGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYKLDGVKPPKKETME
CCCCCCCHHHHHHHHHHHHHCCCEEEEECCHHHHCCCCEEECCCCEECCCCCCCHHHHHH
RVKGILEQYGHKVMF
HHHHHHHHHCCCCCC
>Mature Secondary Structure 
GHIWRNTAMSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWDTHGGKEV
CCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEE
TVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHTCLDTNGFVR
EHHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHH
RYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANKNVKVWIRYVVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEEEEEE
PGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYKLDGVKPPKKETME
CCCCCCCHHHHHHHHHHHHHCCCEEEEECCHHHHCCCCEEECCCCEECCCCCCCHHHHHH
RVKGILEQYGHKVMF
HHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]