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The map label for this gene is insO2 [H]

Identifier: 187732506

GI number: 187732506

Start: 2227197

End: 2228036

Strand: Direct

Name: insO2 [H]

Synonym: SbBS512_E2429

Alternate gene names: 187732506

Gene position: 2227197-2228036 (Clockwise)

Preceding gene: 187734201

Following gene: 187732348

Centisome position: 48.25

GC content: 54.64

Gene sequence:

>840_bases
GTGGTCACACTCTGCCAAGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGG
CAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTACATGGCATCAGCCATGGTTCGGCCGGAGCAAGAAGCATCGCCA
CAATGGCAACCCGGAGAGGCTACCAGATGGGACGCTGGCTTGCTGGCAGGCTCATGAAAGAGCTGGGGCTGGTCAGCTGT
CAGCAGCCGACTCACCGGTATAAACGTGGTGGTCATGAACATGTTGCTATCCCTAACTACCTTGAAAGGCAGTTCGCCGT
GACCGAGCCAAATCAGGTGTGGTGCGGTGATGTGACCTGTATCTGGACGGGTAAGCGCTGGGCGTACCTCGCCGTTGTTC
TCGACCTGTTCGCAAGAAAACCAGTGGGCTGGGCCATGTCGTTCTCGCCGGACAGCAGGCTCACCATGAAAGCGCTGGAA
ATGGCATGGGAAACCCGTGGTAAGCCCGGCGGGGTGATGTTCCACAGCGATCAGGGCAGTCATTATACGAGCAGGCAGTT
CCGGCAGTTATTGTGGCGATACCAGATCAGACAGAGTATGAGCCGGCGCGGAAACTGCTGGGATAACAGCCCAATGGAAC
GCTTCTTCAGGAGTCTGAAGAACGAATGGATGCCGGTGGTGGGTTACGTAAGCTTCAGCGAGGCAGCTCACGCCATAACG
GACTATATCGTTGGATATTACAGCGCACTAAGACCGCACGAATATAACGGTGGGTTACCCCCAAACGAATCGGAAAATCG
ATACTGGAAAAACTCTAACTCGGTGGCCAGTTTTTGTTGA

Upstream 100 bases:

>100_bases
AGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCTGTTCTCGATAATCGGGA
AACTCAGAGCGCATTATCCT

Downstream 100 bases:

>100_bases
CCACTTCAGCCAGAGAAATAACTGGCTGGCTGCTACACCATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAA
AGCTCTGCTGAACAATTGCG

Product: IS911, transposase orfB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MVTLCQVFGVHRSSYRYWKNRPEKPDGRRAVLRSQVLELHGISHGSAGARSIATMATRRGYQMGRWLAGRLMKELGLVSC
QQPTHRYKRGGHEHVAIPNYLERQFAVTEPNQVWCGDVTCIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALE
MAWETRGKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRSLKNEWMPVVGYVSFSEAAHAIT
DYIVGYYSALRPHEYNGGLPPNESENRYWKNSNSVASFC

Sequences:

>Translated_279_residues
MVTLCQVFGVHRSSYRYWKNRPEKPDGRRAVLRSQVLELHGISHGSAGARSIATMATRRGYQMGRWLAGRLMKELGLVSC
QQPTHRYKRGGHEHVAIPNYLERQFAVTEPNQVWCGDVTCIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALE
MAWETRGKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRSLKNEWMPVVGYVSFSEAAHAIT
DYIVGYYSALRPHEYNGGLPPNESENRYWKNSNSVASFC
>Mature_279_residues
MVTLCQVFGVHRSSYRYWKNRPEKPDGRRAVLRSQVLELHGISHGSAGARSIATMATRRGYQMGRWLAGRLMKELGLVSC
QQPTHRYKRGGHEHVAIPNYLERQFAVTEPNQVWCGDVTCIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALE
MAWETRGKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRSLKNEWMPVVGYVSFSEAAHAIT
DYIVGYYSALRPHEYNGGLPPNESENRYWKNSNSVASFC

Specific function: Involved in the transposition of the insertion sequence IS911B [H]

COG id: COG2801

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=245, Percent_Identity=28.1632653061224, Blast_Score=106, Evalue=3e-23,
Organism=Escherichia coli, GI1788405, Length=271, Percent_Identity=32.1033210332103, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1787263, Length=271, Percent_Identity=32.1033210332103, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1786753, Length=271, Percent_Identity=32.1033210332103, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1786570, Length=271, Percent_Identity=32.1033210332103, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1786490, Length=271, Percent_Identity=32.1033210332103, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1789981, Length=267, Percent_Identity=32.2097378277154, Blast_Score=132, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 32270; Mature: 32270

Theoretical pI: Translated: 10.22; Mature: 10.22

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTLCQVFGVHRSSYRYWKNRPEKPDGRRAVLRSQVLELHGISHGSAGARSIATMATRRG
CCCHHHHHHCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCC
YQMGRWLAGRLMKELGLVSCQQPTHRYKRGGHEHVAIPNYLERQFAVTEPNQVWCGDVTC
HHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCHHHHHHEECCCCCCEEECCEEE
IWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALEMAWETRGKPGGVMFHSDQGS
EEECCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCC
HYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRSLKNEWMPVVGYVSFSEAAHAIT
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEEEEHHHHHHHHH
DYIVGYYSALRPHEYNGGLPPNESENRYWKNSNSVASFC
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHCCC
>Mature Secondary Structure
MVTLCQVFGVHRSSYRYWKNRPEKPDGRRAVLRSQVLELHGISHGSAGARSIATMATRRG
CCCHHHHHHCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCC
YQMGRWLAGRLMKELGLVSCQQPTHRYKRGGHEHVAIPNYLERQFAVTEPNQVWCGDVTC
HHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCHHHHHHEECCCCCCEEECCEEE
IWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALEMAWETRGKPGGVMFHSDQGS
EEECCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCC
HYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRSLKNEWMPVVGYVSFSEAAHAIT
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEEEEHHHHHHHHH
DYIVGYYSALRPHEYNGGLPPNESENRYWKNSNSVASFC
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503 [H]