Click here to switch to the map view.

The map label for this gene is insB1 [H]

Identifier: 187733923

GI number: 187733923

Start: 21144

End: 21332

Strand: Direct

Name: insB1 [H]

Synonym: SbBS512_E0022

Alternate gene names: 187733923

Gene position: 21144-21332 (Clockwise)

Preceding gene: 187732489

Following gene: 187732977

Centisome position: 0.46

GC content: 53.44

Gene sequence:

>189_bases
ATGTCGCGCCAGTGCACGCATTATGGGCGTTGGCCTCAACACGGTTTTACGTCACTTAAAAAACTCAGGCCGCAGTCGGT
AACCTCGCGCATACAACCGGGCAGTGATGTGATTGTCTGCGCTGAAATGGACGAACAGTGGGGCTACGTCGGTGCTAAAT
CAGTAAGCGTCAACGGAGCACCGTATTGA

Upstream 100 bases:

>100_bases
GCTCTCATTGCCGTAAAACATGGCAACTACAGTTCACTTACACCGCCTCTCAGCCCGGTACGCACCAGAAAATCATTGAT
ATGGCCATGAATGGCGTCGG

Downstream 100 bases:

>100_bases
CGCTTATTTATTGGTGAGTGCTACGTTCCATGGCAGGAGTTCGTCAACACGGTTGGAAGGCCATTCCGGCAGTACGCTCA
GGATATAGCGCAGATACGCT

Product: IS1 ORF2

Products: NA

Alternate protein names: IS1a [H]

Number of amino acids: Translated: 62; Mature: 61

Protein sequence:

>62_residues
MSRQCTHYGRWPQHGFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSVSVNGAPY

Sequences:

>Translated_62_residues
MSRQCTHYGRWPQHGFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSVSVNGAPY
>Mature_61_residues
SRQCTHYGRWPQHGFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSVSVNGAPY

Specific function: Absolutely required for transposition of IS1 [H]

COG id: COG1662

COG function: function code L; Transposase and inactivated derivatives, IS1 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 27 family [H]

Homologues:

Organism=Escherichia coli, GI1789853, Length=48, Percent_Identity=97.9166666666667, Blast_Score=103, Evalue=2e-24,
Organism=Escherichia coli, GI1788203, Length=48, Percent_Identity=97.9166666666667, Blast_Score=103, Evalue=2e-24,
Organism=Escherichia coli, GI1786203, Length=48, Percent_Identity=97.9166666666667, Blast_Score=103, Evalue=2e-24,
Organism=Escherichia coli, GI1787223, Length=48, Percent_Identity=91.6666666666667, Blast_Score=98, Evalue=1e-22,
Organism=Escherichia coli, GI1786470, Length=48, Percent_Identity=91.6666666666667, Blast_Score=97, Evalue=1e-22,
Organism=Escherichia coli, GI1786459, Length=48, Percent_Identity=91.6666666666667, Blast_Score=97, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005063 [H]

Pfam domain/function: PF03400 Transposase_27 [H]

EC number: NA

Molecular weight: Translated: 6927; Mature: 6796

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRQCTHYGRWPQHGFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSVSVNGA
CCCCCCCCCCCCCCHHHHHHHCCCCCHHHCCCCCCCEEEEEECCCCCCCCCCCEEECCCC
PY
CC
>Mature Secondary Structure 
SRQCTHYGRWPQHGFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSVSVNGA
CCCCCCCCCCCCCHHHHHHHCCCCCHHHCCCCCCCEEEEEECCCCCCCCCCCEEECCCC
PY
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 375010; 1849492; 1630901; 9278503 [H]