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The map label for this gene is insB1 [H]

Identifier: 187733251

GI number: 187733251

Start: 1997026

End: 1997613

Strand: Reverse

Name: insB1 [H]

Synonym: SbBS512_E2198

Alternate gene names: 187733251

Gene position: 1997613-1997026 (Counterclockwise)

Preceding gene: 187733867

Following gene: 187732924

Centisome position: 43.28

GC content: 53.06

Gene sequence:

>588_bases
ATGTCGCGCCAGTGCACGCATTATGGGCGTTGGCCTCAACACGGTTTTCCGTCACTTAAAAAACTCAGGCCGCAGTCGGT
AACCTCGCGCATACAACCGGGCAGTGATGTGATTGTCTGCGCTGAAATGGACGAACAGTGGGGCTACGTCGGTGCTAAAT
CACGTCAGCGCTGGCTGTTTTACGCGTATGACAGGATACGGAGGACGGTTGCGGCGCACGTCTTCGGTGAACGCACTCTG
GCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATGGATGACGGATGGCTGGCCGCTGTATGA
ATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGAGCGACATAATCTGAATCTGAGAC
AACATCTGGCAAGGCTGGGACGGAAGTCACTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAGGTCATCGGGCATTAT
CTGAACATAAAACACTATCAGTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTG
CCGCAGATATTCCCGTGGCGAGCGATAA

Upstream 100 bases:

>100_bases
GCTCTCATTGCCGTAAAACATGGCAACTACAGTTCACTTACACCGCCTCTCAGCCCGGTACGCACCAGAAAATCATTGAT
ATGGCCATGAATGGCGTCGG

Downstream 100 bases:

>100_bases
CCCAGCGCACTATGCGGATGCCATTCGTTATAATGTTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGG
TTTGGGCATGATACTGATGT

Product: IS1 protein InsB

Products: NA

Alternate protein names: IS1a [H]

Number of amino acids: Translated: 195; Mature: 194

Protein sequence:

>195_residues
MSRQCTHYGRWPQHGFPSLKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVAAHVFGERTL
ATLERLLSLLSAFEVVVWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHY
LNIKHYQWICPYISRHLLSLNPLQARCRRYSRGER

Sequences:

>Translated_195_residues
MSRQCTHYGRWPQHGFPSLKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVAAHVFGERTL
ATLERLLSLLSAFEVVVWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHY
LNIKHYQWICPYISRHLLSLNPLQARCRRYSRGER
>Mature_194_residues
SRQCTHYGRWPQHGFPSLKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVAAHVFGERTLA
TLERLLSLLSAFEVVVWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYL
NIKHYQWICPYISRHLLSLNPLQARCRRYSRGER

Specific function: Absolutely required for transposition of IS1 [H]

COG id: COG1662

COG function: function code L; Transposase and inactivated derivatives, IS1 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 27 family [H]

Homologues:

Organism=Escherichia coli, GI1789853, Length=167, Percent_Identity=89.8203592814371, Blast_Score=314, Evalue=3e-87,
Organism=Escherichia coli, GI1788203, Length=167, Percent_Identity=89.8203592814371, Blast_Score=314, Evalue=3e-87,
Organism=Escherichia coli, GI1786203, Length=167, Percent_Identity=89.8203592814371, Blast_Score=314, Evalue=3e-87,
Organism=Escherichia coli, GI1787223, Length=167, Percent_Identity=89.2215568862275, Blast_Score=313, Evalue=3e-87,
Organism=Escherichia coli, GI1786470, Length=161, Percent_Identity=91.9254658385093, Blast_Score=312, Evalue=1e-86,
Organism=Escherichia coli, GI1786459, Length=161, Percent_Identity=91.9254658385093, Blast_Score=312, Evalue=1e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005063 [H]

Pfam domain/function: PF03400 Transposase_27 [H]

EC number: NA

Molecular weight: Translated: 23132; Mature: 23001

Theoretical pI: Translated: 10.92; Mature: 10.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRQCTHYGRWPQHGFPSLKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQRWLF
CCCCCCCCCCCCCCCCCHHHHCCHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCHHHH
YAYDRIRRTVAAHVFGERTLATLERLLSLLSAFEVVVWMTDGWPLYESRLKGKLHVISKR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHCCHHHHHHHH
YTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQWICPYISRHLLSL
HHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCC
NPLQARCRRYSRGER
CHHHHHHHHHCCCCC
>Mature Secondary Structure 
SRQCTHYGRWPQHGFPSLKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQRWLF
CCCCCCCCCCCCCCCCHHHHCCHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCHHHH
YAYDRIRRTVAAHVFGERTLATLERLLSLLSAFEVVVWMTDGWPLYESRLKGKLHVISKR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHCCHHHHHHHH
YTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQWICPYISRHLLSL
HHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCC
NPLQARCRRYSRGER
CHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 375010; 1849492; 1630901; 9278503 [H]