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The map label for this gene is miaB

Identifier: 187733108

GI number: 187733108

Start: 558906

End: 560330

Strand: Direct

Name: miaB

Synonym: SbBS512_E0586

Alternate gene names: 187733108

Gene position: 558906-560330 (Clockwise)

Preceding gene: 187731782

Following gene: 187732299

Centisome position: 12.11

GC content: 54.04

Gene sequence:

>1425_bases
ATGACCAAAAAACTCCATATTAAAACCTGGGGCTGTCAGATGAACGAGTACGATTCATCGAAGATGGCCGATCTGCTGGA
TGCCACCCACAGCTATCAACTGACCGACGTGGCGGAAGAAGCGGATGTGCTGCTGCTGAACACCTGCTCAATCCGCGAGA
AGGCTCAGGAAAAAGTCTTCCATCAGTTGGGTCGCTGGAAACTGTTAAAAGAGAAGAATCCAGACCTGATTATCGGCGTC
GGTGGCTGCGTGGCATCGCAAGAAGGCGAGCACATTCGCCAGCGCGCCCACTATGTCGATATTATTTTTGGGCCGCAAAC
GCTGCACCGTCTGCCGGAGATGATCAACTCCGTGCGCGGCGACCGCAGCCCGGTTGTAGATATCAGCTTCCCGGAAATCG
AGAAGTTTGACCGTCTACCGGAACCGCGCGCCGAAGGGCCGACCGCGTTTGTCTCCATCATGGAAGGCTGCAATAAATAT
TGCACCTACTGCGTGGTGCCTTACACCCGTGGTGAAGAGGTAAGCCGTCCGTCTGACGATATTCTGTTTGAGATTGCCCA
GCTTGCGGCTCAGGGCGTGCGTGAAGTCAACCTGCTCGGTCAGAACGTGAACGCCTGGCGTGGTGAAAACTACGACGGCT
CCACCGGATCGTTTGCCGATCTGCTGCGTCTGGTTGCTGCGATCGACGGGATCGATCGTATTCGCTTTACCACCAGCCAT
CCGATCGAATTCACCGACGATATCATCGAAGTGTATCGCGACACGCCGGAGCTGGTGAGCTTCCTGCATCTGCCGGTACA
GAGCGGTTCCGATCGCATTCTGAACCTGATGGGCCGTACTCATACGGCACTGGAGTACAAAGCGATCATCCGTAAACTGC
GTGCGGCGCGTCCAGATATTCAGATCAGTTCTGATTTCATCGTTGGCTTCCCTGGCGAAACCACCGAAGACTTCGAGAAA
ACGATGAAGCTGATTGCCGACGTCAATTTCGACATGAGCTACAGCTTTATCTTCTCTGCACGTCCGGGTACACCAGCCGC
CGATATGGTTGATGATGTTCCGGAAGAAGAGAAGAAGCAGCGTCTGTATATTCTGCAAGAACGTATCAATCAGCAAGCGA
TGGCATGGAGCCGCCGGATGCTCGGCACCACCCAGCGTATTCTGGTAGAAGGGACTTCGCGTAAGAGCATCATGGAGCTT
TCCGGGCGTACCGAAAATAACCGCGTGGTCAACTTCGAGGGCACACCGGATATGATCGGTAAATTCGTCGATGTAGAAAT
TACCGACGTCTACCCGAACTCTCTGCGCGGTAAAGTGGTGCGTACTGAAGATGAAATGGGCCTGCGCGTGGCAGAAACAC
CGGAATCGGTGATTGCCCGTACCCGCAAAGAAAACGACCTTGGCGTGGGTTATTATCAGCCGTAA

Upstream 100 bases:

>100_bases
TACCCCCAAGGGCTGATAACGCTCATACTGGCCACAACGGCAGCAAAGCATTACACTATGCGCCCTGCATTCCTGGCTAC
TATTTCGCAAGAGCAAGTCG

Downstream 100 bases:

>100_bases
TTCTCAGGCCCGCCGTTCCGGTGGGCCTTGTTTTTTCTCTCTTTATCCCCATCTTTATCGCAATGCTTGCGCCTTTTTCC
TGTGGCGTGAATAATTTCCT

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MTKKLHIKTWGCQMNEYDSSKMADLLDATHSYQLTDVAEEADVLLLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGV
GGCVASQEGEHIRQRAHYVDIIFGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGSTGSFADLLRLVAAIDGIDRIRFTTSH
PIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNLMGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEK
TMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGTSRKSIMEL
SGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRVAETPESVIARTRKENDLGVGYYQP

Sequences:

>Translated_474_residues
MTKKLHIKTWGCQMNEYDSSKMADLLDATHSYQLTDVAEEADVLLLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGV
GGCVASQEGEHIRQRAHYVDIIFGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGSTGSFADLLRLVAAIDGIDRIRFTTSH
PIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNLMGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEK
TMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGTSRKSIMEL
SGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRVAETPESVIARTRKENDLGVGYYQP
>Mature_473_residues
TKKLHIKTWGCQMNEYDSSKMADLLDATHSYQLTDVAEEADVLLLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVG
GCVASQEGEHIRQRAHYVDIIFGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYC
TYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGSTGSFADLLRLVAAIDGIDRIRFTTSHP
IEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNLMGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKT
MKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGTSRKSIMELS
GRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRVAETPESVIARTRKENDLGVGYYQP

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=481, Percent_Identity=37.2141372141372, Blast_Score=290, Evalue=2e-78,
Organism=Homo sapiens, GI28872782, Length=478, Percent_Identity=37.2384937238494, Blast_Score=290, Evalue=3e-78,
Organism=Homo sapiens, GI93277076, Length=456, Percent_Identity=30.2631578947368, Blast_Score=177, Evalue=2e-44,
Organism=Escherichia coli, GI1786882, Length=474, Percent_Identity=99.57805907173, Blast_Score=981, Evalue=0.0,
Organism=Escherichia coli, GI1787057, Length=385, Percent_Identity=28.051948051948, Blast_Score=147, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17553146, Length=462, Percent_Identity=32.9004329004329, Blast_Score=244, Evalue=9e-65,
Organism=Caenorhabditis elegans, GI71996771, Length=345, Percent_Identity=28.1159420289855, Blast_Score=118, Evalue=8e-27,
Organism=Drosophila melanogaster, GI21356207, Length=433, Percent_Identity=36.9515011547344, Blast_Score=276, Evalue=2e-74,
Organism=Drosophila melanogaster, GI19922432, Length=493, Percent_Identity=28.6004056795132, Blast_Score=160, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 53680; Mature: 53548

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKLHIKTWGCQMNEYDSSKMADLLDATHSYQLTDVAEEADVLLLNTCSIREKAQEKVF
CCCEEEEEEECCCCCCCCHHHHHHHHHHHCCEEEHHHHCCCCEEEEECCHHHHHHHHHHH
HQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDIIFGPQTLHRLPEMINSVRG
HHHCCEEEECCCCCCEEEECCCCCCCCCHHHHHHHCCEEEEEECHHHHHHHHHHHHHHCC
DRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYCTYCVVPYTRGEEVSRPSDD
CCCCEEECCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCHH
ILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGSTGSFADLLRLVAAIDGIDRIRFTTSH
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHEEEECCC
PIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNLMGRTHTALEYKAIIRKLRAARPDI
CCCHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCCCC
QISSDFIVGFPGETTEDFEKTMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQ
EECCCEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHCCCCHHHHH
RLYILQERINQQAMAWSRRMLGTTQRILVEGTSRKSIMELSGRTENNRVVNFEGTPDMIG
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHCCCCCCCEEEEECCCHHHHC
KFVDVEITDVYPNSLRGKVVRTEDEMGLRVAETPESVIARTRKENDLGVGYYQP
CEEEEEEEECCCCCCCCCEEEEHHHCCCEEECCCHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
TKKLHIKTWGCQMNEYDSSKMADLLDATHSYQLTDVAEEADVLLLNTCSIREKAQEKVF
CCEEEEEEECCCCCCCCHHHHHHHHHHHCCEEEHHHHCCCCEEEEECCHHHHHHHHHHH
HQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDIIFGPQTLHRLPEMINSVRG
HHHCCEEEECCCCCCEEEECCCCCCCCCHHHHHHHCCEEEEEECHHHHHHHHHHHHHHCC
DRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYCTYCVVPYTRGEEVSRPSDD
CCCCEEECCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCHH
ILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGSTGSFADLLRLVAAIDGIDRIRFTTSH
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHEEEECCC
PIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNLMGRTHTALEYKAIIRKLRAARPDI
CCCHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCCCC
QISSDFIVGFPGETTEDFEKTMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQ
EECCCEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHCCCCHHHHH
RLYILQERINQQAMAWSRRMLGTTQRILVEGTSRKSIMELSGRTENNRVVNFEGTPDMIG
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHCCCCCCCEEEEECCCHHHHC
KFVDVEITDVYPNSLRGKVVRTEDEMGLRVAETPESVIARTRKENDLGVGYYQP
CEEEEEEEECCCCCCCCCEEEEHHHCCCEEECCCHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA