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The map label for this gene is rstB

Identifier: 187731289

GI number: 187731289

Start: 1638977

End: 1640278

Strand: Direct

Name: rstB

Synonym: SbBS512_E1795

Alternate gene names: 187731289

Gene position: 1638977-1640278 (Clockwise)

Preceding gene: 187731773

Following gene: 187734206

Centisome position: 35.51

GC content: 50.61

Gene sequence:

>1302_bases
ATGAAAAAACTGTTTATCCAGTTTTACCTGTTATTGTTTGTCTGCTTCCTTGTGATGTCTCTGCTGGTTGGGTTGGTGTA
CAAATTTACCGCCGAACGCGCGGGCAAACAGTCGCTGGATGATTTGATGAACAGTTTGCTGTATCTGATGCGCAGCGAAT
TGCGTGAGATCCCCCCACACGACTGGGGTAAGACGCTGAAAGAGATGGATTTAAATCTCTCTTTCGATCTGCGTGTCGAG
CCACTGAGTAAATACCATCTTGATGATATTTCCATGCACCGACTGCGTGGCGGCGAAATTGTCGCCCTGGACGATCAGTA
CACGTTTTTGCAGCGTATCCCGCGCAGCCACTACGTGCTGGCAGTTGGTCCTGTTCCTTATCTTTATTATCTCCATCAGA
TGCGATTGCTGGATATCGCCCTGATCGCTTTTATTGCTATTTCCCTCGCCTTTCCGGTGTTTATCTGGATGCGTCCGCAC
TGGCAGGATATGTTAAAACTGGAAGCAGCGGCGCAACGATTTGGCGATGGGCATCTCTATGAGCGTATCCACTTTGATGA
GGGTTCGAGCTTTGAACGACTTGGTATCGCATTTAACCAGATGGCGGACAATATCAACGCCTTGATTGCCAGCAAAAAAC
AGCTTATTGACGGTATCGCTCACGAACTGCGAACACCGTTAGTGCGCCTGTGTTATCGACTGGAGATGAGCGATAACCTG
AGCGCCGCCGAGTCCCAGGCGTTGAATCGTGATATCAGTCAACTTGAAGCTTTAATTGAAGAGCTGCTGACTTATGCCCG
ACTCGATCGCCCACAAAACGAGCTTCATCTTAGCGAACCAGACCTGCCGTTGTGGCTGTCAACGCATCTGGCAGATATTC
AGGCAGTAACGCCCGATAAAACGGTACGGATAAAAACGCTCGTGCAAGGCCATTATGCGGCGTTGGATATGCGCTTAATG
GAGCGCGTGCTGGATAATTTGCTCAATAACGCCCTGCGGTACTGCCATTCAACGGTTGAAACCAGCCTGCTACTGTCGGG
GAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTG
TTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCA
ATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCTCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACAT
CCCGCAATTTACCTCTGCCTGA

Upstream 100 bases:

>100_bases
GTTAAGAAAAAAACTGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGC
CTCATGCATGGGAATAAGCG

Downstream 100 bases:

>100_bases
CACTACGTGCACGATGGTGAAGTCACCACCATTGTGCTATAAATTAAGGATGTTGTAACTAAAGTGGTTAATATTATGGC
GCGTTACGATCTCGTAGACC

Product: sensor protein RstB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMNSLLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVE
PLSKYHLDDISMHRLRGGEIVALDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
WQDMLKLEAAAQRFGDGHLYERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDGIAHELRTPLVRLCYRLEMSDNL
SAAESQALNRDISQLEALIEELLTYARLDRPQNELHLSEPDLPLWLSTHLADIQAVTPDKTVRIKTLVQGHYAALDMRLM
ERVLDNLLNNALRYCHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLAIVHSIALA
MGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA

Sequences:

>Translated_433_residues
MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMNSLLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVE
PLSKYHLDDISMHRLRGGEIVALDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
WQDMLKLEAAAQRFGDGHLYERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDGIAHELRTPLVRLCYRLEMSDNL
SAAESQALNRDISQLEALIEELLTYARLDRPQNELHLSEPDLPLWLSTHLADIQAVTPDKTVRIKTLVQGHYAALDMRLM
ERVLDNLLNNALRYCHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLAIVHSIALA
MGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA
>Mature_433_residues
MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMNSLLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVE
PLSKYHLDDISMHRLRGGEIVALDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
WQDMLKLEAAAQRFGDGHLYERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDGIAHELRTPLVRLCYRLEMSDNL
SAAESQALNRDISQLEALIEELLTYARLDRPQNELHLSEPDLPLWLSTHLADIQAVTPDKTVRIKTLVQGHYAALDMRLM
ERVLDNLLNNALRYCHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLAIVHSIALA
MGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA

Specific function: Member of the two-component regulatory system rstB/rstA. RstB functions as a membrane-associated protein kinase that phosphorylates rstA (Potential) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787894, Length=433, Percent_Identity=99.0762124711316, Blast_Score=879, Evalue=0.0,
Organism=Escherichia coli, GI1790346, Length=272, Percent_Identity=34.5588235294118, Blast_Score=138, Evalue=5e-34,
Organism=Escherichia coli, GI1789808, Length=265, Percent_Identity=32.0754716981132, Blast_Score=131, Evalue=8e-32,
Organism=Escherichia coli, GI1786783, Length=246, Percent_Identity=33.739837398374, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1786600, Length=228, Percent_Identity=31.140350877193, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1789403, Length=236, Percent_Identity=31.3559322033898, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1788393, Length=269, Percent_Identity=24.1635687732342, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI145693157, Length=254, Percent_Identity=23.6220472440945, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787374, Length=283, Percent_Identity=26.8551236749117, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1786912, Length=259, Percent_Identity=28.5714285714286, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI87081816, Length=227, Percent_Identity=28.1938325991189, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1788279, Length=278, Percent_Identity=28.7769784172662, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1789149, Length=227, Percent_Identity=26.8722466960352, Blast_Score=66, Evalue=6e-12,
Organism=Escherichia coli, GI1788713, Length=234, Percent_Identity=24.3589743589744, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI87082128, Length=248, Percent_Identity=25.8064516129032, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 49320; Mature: 49320

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMNSLLYLMRSELREIPPH
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCC
DWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVALDDQYTFLQRIPRSHYVL
HHHHHHHHHCCCCEEEEEECCHHHHCCCCHHHHHCCCCCEEEECCCHHHHHHCCCCCEEE
AVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPHWQDMLKLEAAAQRFGDGHLY
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHH
ERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDGIAHELRTPLVRLCYRLEMSDNL
HEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SAAESQALNRDISQLEALIEELLTYARLDRPQNELHLSEPDLPLWLSTHLADIQAVTPDK
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEHHHHHHHHEEECCCC
TVRIKTLVQGHYAALDMRLMERVLDNLLNNALRYCHSTVETSLLLSGNRATLIVEDDGPG
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCEEEEEEECCCCC
IAPENREHIFEPFVRLDPSRDRSTGGCGLGLAIVHSIALAMGGTVNCDTSELGGARFSFS
CCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHCCCCEEEEC
WPLWHNIPQFTSA
CCHHCCCCCCCCC
>Mature Secondary Structure
MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMNSLLYLMRSELREIPPH
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCC
DWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVALDDQYTFLQRIPRSHYVL
HHHHHHHHHCCCCEEEEEECCHHHHCCCCHHHHHCCCCCEEEECCCHHHHHHCCCCCEEE
AVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPHWQDMLKLEAAAQRFGDGHLY
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHH
ERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDGIAHELRTPLVRLCYRLEMSDNL
HEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SAAESQALNRDISQLEALIEELLTYARLDRPQNELHLSEPDLPLWLSTHLADIQAVTPDK
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEHHHHHHHHEEECCCC
TVRIKTLVQGHYAALDMRLMERVLDNLLNNALRYCHSTVETSLLLSGNRATLIVEDDGPG
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCEEEEEEECCCCC
IAPENREHIFEPFVRLDPSRDRSTGGCGLGLAIVHSIALAMGGTVNCDTSELGGARFSFS
CCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHCCCCEEEEC
WPLWHNIPQFTSA
CCHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 2646639; 1925016; 1495392 [H]