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The map label for this gene is puuP [H]

Identifier: 187731252

GI number: 187731252

Start: 1399255

End: 1400640

Strand: Reverse

Name: puuP [H]

Synonym: SbBS512_E1532

Alternate gene names: 187731252

Gene position: 1400640-1399255 (Counterclockwise)

Preceding gene: 187731037

Following gene: 187731790

Centisome position: 30.34

GC content: 53.17

Gene sequence:

>1386_bases
ATGGCTATTAATTCACCACTGAATATTGCTGCGCAACCCGGCAAAACCCGTCTGCGAAAATCACTGAAATTGTGGCAGGT
GGTGATGATGGGTCTGGCCTATCTCACGCCGATGACCGTATTTGATACTTTTGGTATTGTCTCCGGCATTAGCGACGGTC
ACGTTCCGGCGTCCTATTTGCTGGCGCTGGCGGGCGTGCTGTTTACCGCTATCAGCTACGGCAAACTGGTTCGCCAGTTT
CCGGAGGCCGATTCGGCCTATACCTACGCGCAAAAGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCT
GCTGGATTATCTCTTTTTGCCGATGATCAACGTCTTGTTGGCGAAAATCTATCTCTCCGCCCTCTTCCCGGAAGTGCCGC
CGTGGGTGTGCGTAGTAACCTTCGTCGCCATTTTAACCGCCGCGAATCTGAAGAGCATCAACCTAGTCGCTAACTTCAAT
ACCCTGTTTGTACTGGTGCAAATCTCCATCATGGTGGTGTTTATCTTCCTGGTAGTTCAGGGACTGCATAAAGGAGAAGG
CGTTGGCACCGTCTGGTCACTTCAGCCGTTTATCAGCGAGAACGCGCACCTGATCCCGATTATTACCGGGGCGACGATTG
TCTGTTTCTCCTTCCTCGGTTTCGATGCGGTGACCACGCTTTCGGAAGAGACGCCGGACGCCGCACGCGTGATCCCGAAA
GCCATCTTCCTGACGGCGGTCTACGGTGGCGTTATCTTTATCGCGGCGTCGTTCTTTATGCAGCTGTTCTTTCCCGATAT
CAGCCGCTTTAAAGATCCGGATGCCGCACTGCCTGAAATTGCGCTGTACGTCGGCGGCAAGCTCTTCCAGTCGATTTTCC
TCTGCACCACGTTTGTGAACACGTTAGCCTCTGGCCTGGCTTCACATGCCAGCGTGTCGCGTCTGCTGTATGTGATGGGG
CGTGACAATGTGTTTCCGGAGCGCGTGTTTGGCTATGTGCACCCAAAATGGCGGACTCCGGCACTCAACGTCATTATGGT
CGGGATTGTCGCGTTGTCGGCGCTGTTCTTCGATTTAGTTACCGCCACAGCGTTAATTAACTTCGGTGCTCTGGTGGCGT
TTACCTTCGTGAATCTGTCGGTGTTTAATCATTTCTGGCGGCGTAAAGGAATGAATAAAAGCTGGAAGGATCACTTCCAC
TATTTGCTGATGCCGCTGGTTGGCGCGCTGACGGTGGGTGTGCTGTGGGTTAACCTCGAGTCAACGTCACTGACACTCGG
TCTGGTATGGGCTTCGTTAGGCGGCGCATATTTATGGTATCTGATCCGCCGCTATCGCAAAGTGCCGTTGTATGATGGTG
ACAGAACGCCGGTGAGTGAAACGTAA

Upstream 100 bases:

>100_bases
GCTACTGACTGGACTCCGTCCCCTTTGCTTCCCTTGCTAAACAGGTTTGATTTCAGGTCGACACGACCGCAAACCTTATT
ACGCAGGGGAGGCAGCAATT

Downstream 100 bases:

>100_bases
TAGTCGGATGCGCGGCACATTATCCTGCCTGAGGGTAATGCACCGCGCCATTAACTATAAAAAGATGTATGACCCGGATC
ACGAGTGCTATCATCCGCCC

Product: amino acid permease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTFGIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQF
PEADSAYTYAQKSINPHVGFMVGWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVCVVTFVAILTAANLKSINLVANFN
TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSFLGFDAVTTLSEETPDAARVIPK
AIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAALPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMG
RDNVFPERVFGYVHPKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMNKSWKDHFH
YLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPLYDGDRTPVSET

Sequences:

>Translated_461_residues
MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTFGIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQF
PEADSAYTYAQKSINPHVGFMVGWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVCVVTFVAILTAANLKSINLVANFN
TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSFLGFDAVTTLSEETPDAARVIPK
AIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAALPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMG
RDNVFPERVFGYVHPKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMNKSWKDHFH
YLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPLYDGDRTPVSET
>Mature_460_residues
AINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTFGIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFP
EADSAYTYAQKSINPHVGFMVGWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVCVVTFVAILTAANLKSINLVANFNT
LFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSFLGFDAVTTLSEETPDAARVIPKA
IFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAALPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGR
DNVFPERVFGYVHPKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMNKSWKDHFHY
LLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPLYDGDRTPVSET

Specific function: Imports putrescine [H]

COG id: COG0531

COG function: function code E; Amino acid transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily [H]

Homologues:

Organism=Homo sapiens, GI114326544, Length=422, Percent_Identity=22.5118483412322, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI114326550, Length=422, Percent_Identity=22.5118483412322, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI4507047, Length=415, Percent_Identity=21.9277108433735, Blast_Score=72, Evalue=8e-13,
Organism=Escherichia coli, GI87081869, Length=461, Percent_Identity=99.3492407809111, Blast_Score=921, Evalue=0.0,
Organism=Escherichia coli, GI87082023, Length=446, Percent_Identity=63.4529147982063, Blast_Score=576, Evalue=1e-165,
Organism=Escherichia coli, GI1788480, Length=395, Percent_Identity=23.5443037974684, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1786602, Length=176, Percent_Identity=24.4318181818182, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1786789, Length=175, Percent_Identity=26.8571428571429, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI87081708, Length=467, Percent_Identity=23.982869379015, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI87082250, Length=394, Percent_Identity=22.3350253807107, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI1786302, Length=173, Percent_Identity=24.8554913294798, Blast_Score=62, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI17532491, Length=408, Percent_Identity=23.5294117647059, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17533459, Length=404, Percent_Identity=23.5148514851485, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24667468, Length=400, Percent_Identity=25.5, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24668806, Length=418, Percent_Identity=24.6411483253589, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21356285, Length=418, Percent_Identity=24.6411483253589, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24668802, Length=418, Percent_Identity=24.6411483253589, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI281366235, Length=412, Percent_Identity=24.7572815533981, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI116007820, Length=412, Percent_Identity=24.7572815533981, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI116007818, Length=412, Percent_Identity=24.2718446601942, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI85725152, Length=412, Percent_Identity=24.2718446601942, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI221512776, Length=396, Percent_Identity=20.7070707070707, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24666159, Length=396, Percent_Identity=20.7070707070707, Blast_Score=79, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004841
- InterPro:   IPR002293 [H]

Pfam domain/function: PF00324 AA_permease [H]

EC number: NA

Molecular weight: Translated: 50843; Mature: 50711

Theoretical pI: Translated: 9.43; Mature: 9.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTFGIVSGISDGHVPASYL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
LALAGVLFTAISYGKLVRQFPEADSAYTYAQKSINPHVGFMVGWSSLLDYLFLPMINVLL
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AKIYLSALFPEVPPWVCVVTFVAILTAANLKSINLVANFNTLFVLVQISIMVVFIFLVVQ
HHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHH
GLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSFLGFDAVTTLSEETPDAARVIPK
HHCCCCCCCEEEECCHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
AIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAALPEIALYVGGKLFQSIFLCTTFVN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
TLASGLASHASVSRLLYVMGRDNVFPERVFGYVHPKWRTPALNVIMVGIVALSALFFDLV
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
TATALINFGALVAFTFVNLSVFNHFWRRKGMNKSWKDHFHYLLMPLVGALTVGVLWVNLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEC
STSLTLGLVWASLGGAYLWYLIRRYRKVPLYDGDRTPVSET
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTFGIVSGISDGHVPASYL
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
LALAGVLFTAISYGKLVRQFPEADSAYTYAQKSINPHVGFMVGWSSLLDYLFLPMINVLL
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AKIYLSALFPEVPPWVCVVTFVAILTAANLKSINLVANFNTLFVLVQISIMVVFIFLVVQ
HHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHH
GLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSFLGFDAVTTLSEETPDAARVIPK
HHCCCCCCCEEEECCHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
AIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAALPEIALYVGGKLFQSIFLCTTFVN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
TLASGLASHASVSRLLYVMGRDNVFPERVFGYVHPKWRTPALNVIMVGIVALSALFFDLV
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
TATALINFGALVAFTFVNLSVFNHFWRRKGMNKSWKDHFHYLLMPLVGALTVGVLWVNLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEC
STSLTLGLVWASLGGAYLWYLIRRYRKVPLYDGDRTPVSET
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]