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The map label for this gene is hisH

Identifier: 187730914

GI number: 187730914

Start: 1116348

End: 1116938

Strand: Reverse

Name: hisH

Synonym: SbBS512_E1209

Alternate gene names: 187730914

Gene position: 1116938-1116348 (Counterclockwise)

Preceding gene: 187733376

Following gene: 187730346

Centisome position: 24.2

GC content: 56.18

Gene sequence:

>591_bases
ATGAACGTGGTGATCCTTGATACCGGCTGCGCCAATCTGAACTCGGTGAAGTCAGCCATTGCGCGCCACGGTTATGAACC
AAAAGTCAGCCGTGACCCGGACGTGGTGTTGCTGGCCGATAAACTATTTTTACCCGGCGTTGGCACTGCGCAAGCAGCGA
TGGATCAGGTGCGTGAGCGCGAGCTGTTTGATCTCATCAAAGCCTGTACCCAACCGGTACTGGGCATCTGCTTAGGGATG
CAACTGCTGGGTCGGCGCAGCGAAGAGAGCAACGGCGTCGACTTGCTGGGCATCATCGACGAAGACGTGCCGAAAATGAC
CGACTTTGGTCTGCCGCTGCCGCATATGGGCTGGAATCGCGTTTACCCGCAGGCAGGCAACCGCCTGTTTCAGGGGATTG
AAGACGGCGCGTACTTTTACTTTGTTCACAGCTACGCAATGCCAGTCAATCCGTGGACCATCGCCCAGTGTAATTACGGC
GAACCGTTCACCGCGGCGGTACAAAAAGATAACTTCTACGGCGTGCAGTTCCACCCGGAGCGTTCTGGTGCCGCTGGCGC
TAAGTTGCTGAAAAACTTCCTGGAGATGTGA

Upstream 100 bases:

>100_bases
ACCACCGTGTAGAGAGCCTGTTCAAAGCCTTTGGTCGCACCCTGCGCCAGGCCATCCGCGTGGAAGGCGACACCCTGCCC
TCGTCGAAAGGAGTGCTGTA

Downstream 100 bases:

>100_bases
TGATTATTCCGGCATTAGATTTAATCGACGGCACCGTGGTGCGTCTCCATCAGGGCGATTACGGCAAACAACGCGATTAC
GGTAACGACCCGCTGCCGCG

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 196; Mature: 196

Protein sequence:

>196_residues
MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFLPGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGM
QLLGRRSEESNGVDLLGIIDEDVPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM

Sequences:

>Translated_196_residues
MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFLPGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGM
QLLGRRSEESNGVDLLGIIDEDVPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM
>Mature_196_residues
MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFLPGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGM
QLLGRRSEESNGVDLLGIIDEDVPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=196, Percent_Identity=100, Blast_Score=409, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6319725, Length=209, Percent_Identity=35.4066985645933, Blast_Score=120, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 21653; Mature: 21653

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00442 GATASE_TYPE_I ; PS00136 SUBTILASE_ASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFLPGVGTAQAAMDQVRER
CEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEECCEECCCCCHHHHHHHHHHHH
ELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDEDVPKMTDFGLPLPHMGWNR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHCCCCCCCCCCCC
VYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYGEPFTAAVQKDNFYGVQFHPE
CCCHHHHHHHHCCCCCEEEEEEEEECCCCCCEEEEECCCCCCEEEEEECCCEEEEEECCC
RSGAAGAKLLKNFLEM
CCCCHHHHHHHHHHCC
>Mature Secondary Structure
MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFLPGVGTAQAAMDQVRER
CEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEECCEECCCCCHHHHHHHHHHHH
ELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDEDVPKMTDFGLPLPHMGWNR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHCCCCCCCCCCCC
VYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYGEPFTAAVQKDNFYGVQFHPE
CCCHHHHHHHHCCCCCEEEEEEEEECCCCCCEEEEECCCCCCEEEEEECCCEEEEEECCC
RSGAAGAKLLKNFLEM
CCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10222209; 11206551; 11258796 [H]