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The map label for this gene is insB1 [H]

Identifier: 187730369

GI number: 187730369

Start: 4441749

End: 4441916

Strand: Reverse

Name: insB1 [H]

Synonym: SbBS512_E4759

Alternate gene names: 187730369

Gene position: 4441916-4441749 (Counterclockwise)

Preceding gene: 187732988

Following gene: 187731736

Centisome position: 96.23

GC content: 47.62

Gene sequence:

>168_bases
TTGTCTGGAGATTCAGGGGGCCAGTCTACTCAGCGCATTGAGCGACATAATCTGAATCTGAGACAACATCTGGCAAGGCT
GGGACGGAAGTCACTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAGGTCATCGGGCATTATCTGAACATAAAACACT
ATCAGTAA

Upstream 100 bases:

>100_bases
GCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTACTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATC
ACTTATTTAAGTGATACTGG

Downstream 100 bases:

>100_bases
GTTGGAGTCATTACCAATTTTTGCACTTTAAATACAATCTCTTTTTATTTATAACTTTTTAGGTTGGGAGAAATCAGGAT
GGCAGAATCAGGAAATACAC

Product: hypothetical protein

Products: NA

Alternate protein names: IS1a [H]

Number of amino acids: Translated: 55; Mature: 54

Protein sequence:

>55_residues
MSGDSGGQSTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ

Sequences:

>Translated_55_residues
MSGDSGGQSTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ
>Mature_54_residues
SGDSGGQSTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ

Specific function: Absolutely required for transposition of IS1 [H]

COG id: COG1662

COG function: function code L; Transposase and inactivated derivatives, IS1 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 27 family [H]

Homologues:

Organism=Escherichia coli, GI1789853, Length=46, Percent_Identity=100, Blast_Score=96, Evalue=3e-22,
Organism=Escherichia coli, GI1788203, Length=46, Percent_Identity=100, Blast_Score=96, Evalue=3e-22,
Organism=Escherichia coli, GI1786203, Length=46, Percent_Identity=100, Blast_Score=96, Evalue=3e-22,
Organism=Escherichia coli, GI1787223, Length=46, Percent_Identity=97.8260869565217, Blast_Score=95, Evalue=1e-21,
Organism=Escherichia coli, GI1786470, Length=46, Percent_Identity=97.8260869565217, Blast_Score=94, Evalue=2e-21,
Organism=Escherichia coli, GI1786459, Length=46, Percent_Identity=97.8260869565217, Blast_Score=94, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005063 [H]

Pfam domain/function: PF03400 Transposase_27 [H]

EC number: NA

Molecular weight: Translated: 6342; Mature: 6211

Theoretical pI: Translated: 10.88; Mature: 10.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGDSGGQSTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ
CCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SGDSGGQSTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ
CCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 375010; 1849492; 1630901; 9278503 [H]