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The map label for this gene is cpdB [H]

Identifier: 187731736

GI number: 187731736

Start: 4437505

End: 4438164

Strand: Reverse

Name: cpdB [H]

Synonym: SbBS512_E4754

Alternate gene names: 187731736

Gene position: 4438164-4437505 (Counterclockwise)

Preceding gene: 187730369

Following gene: 187733978

Centisome position: 96.15

GC content: 48.64

Gene sequence:

>660_bases
TTGACCTTCCGTAATGCCGCCGATCTTTATCTCTACCCCAATACGCTGATTGTAGTGAAAGCCAGCGGTAAAGAGGTGAA
AGAGTGGCTGGAGTGCTCCGCCGGACAGTTTAACCAGATTGATCCTAACAGCACGAAACCACAGTCACTCATCAACTGGG
ATGGTTTCCGCACTTATAACTTTGATGTTATTGATGGTGTGAATTATCAGATTGATGTTACCCAACCCGCCCGCTATGAC
GGCGAGTGCCAGATGATTAATGCCAATGCAGAGCGGATTAAGAACCTGACCTTTAATGGCAAGCCGATTGATCCGAACGC
TATGTTCCTCGTTGCCACCAATAACTATCGCGCTTACGGCGGCAAATTTGCCGGTACGGGCGACAGCCATATCGCTTTTG
CTTCACCGGATGAGAATCGCTCGGTGCTGGCAGCGAGGATTGCTGATGAGTCGAAACGTGCGGGAGAAATTCACCCGGCG
GCAGATAACAACTGGCGTTTAGCACCGATAGCTGGCGATAAGAAACTGGATATCCGTTTCGAAACTTCCCCGTCAGATAA
AGCCGCAGCGTTTATTAAAGAGAAAGGGCAATATCCGATGAATAAAGTCGCGACCGATGATATCGGGTTTGCGATTTATC
AGGTGGATTTGAGTAAGTAA

Upstream 100 bases:

>100_bases
AATTTCTTTTTTTCATTGGAGCACCTGTGTTGTTCTGAGGTGAGCATATCACCTCTGTTCAGGTGGCCAAATTCAGTGTG
CCACTTCAGAAAAAGGCCAG

Downstream 100 bases:

>100_bases
AACACTTCTTTTTCGGTCTATAAATTATCAACCGCATCCGGCATTTATTGGCGGATGCGATGCTGGCGCATCTTATCCGC
CCTACAAGCCATGCACCGTA

Product: 2',3'-cyclic-nucleotide 2'-phosphodiesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 219; Mature: 218

Protein sequence:

>219_residues
MTFRNAADLYLYPNTLIVVKASGKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDENRSVLAARIADESKRAGEIHPA
ADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKGQYPMNKVATDDIGFAIYQVDLSK

Sequences:

>Translated_219_residues
MTFRNAADLYLYPNTLIVVKASGKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDENRSVLAARIADESKRAGEIHPA
ADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKGQYPMNKVATDDIGFAIYQVDLSK
>Mature_218_residues
TFRNAADLYLYPNTLIVVKASGKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYDG
ECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDENRSVLAARIADESKRAGEIHPAA
DNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKGQYPMNKVATDDIGFAIYQVDLSK

Specific function: This bifunctional enzyme catalyzes two consecutive reactions during ribonucleic acid degradation. Converts a 2',3'- cyclic nucleotide to a 3'-nucleotide and then the 3'-nucleotide to the corresponding nucleoside and phosphate [H]

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Escherichia coli, GI1790658, Length=219, Percent_Identity=99.0867579908676, Blast_Score=455, Evalue=1e-129,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR006294
- InterPro:   IPR004843 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16 [H]

Molecular weight: Translated: 24326; Mature: 24195

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFRNAADLYLYPNTLIVVKASGKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYN
CCCCCCCEEEECCCEEEEEEECCHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCCEEEEE
FDVIDGVNYQIDVTQPARYDGECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYG
EEEECCCCEEEEECCCCCCCCEEEEEECCHHHHHCCCCCCCCCCCCCEEEEEECCEEEEC
GKFAGTGDSHIAFASPDENRSVLAARIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRF
CEECCCCCCEEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEE
ETSPSDKAAAFIKEKGQYPMNKVATDDIGFAIYQVDLSK
ECCCCHHHHHHHHHCCCCCCHHCCCCCCCEEEEEEEECC
>Mature Secondary Structure 
TFRNAADLYLYPNTLIVVKASGKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYN
CCCCCCEEEECCCEEEEEEECCHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCCEEEEE
FDVIDGVNYQIDVTQPARYDGECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYG
EEEECCCCEEEEECCCCCCCCEEEEEECCHHHHHCCCCCCCCCCCCCEEEEEECCEEEEC
GKFAGTGDSHIAFASPDENRSVLAARIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRF
CEECCCCCCEEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEE
ETSPSDKAAAFIKEKGQYPMNKVATDDIGFAIYQVDLSK
ECCCCHHHHHHHHHCCCCCCHHCCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3005231; 7610040; 9278503; 2172762; 9298646 [H]