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The map label for this gene is nuoB

Identifier: 187736633

GI number: 187736633

Start: 2629441

End: 2630022

Strand: Direct

Name: nuoB

Synonym: Amuc_2157

Alternate gene names: 187736633

Gene position: 2629441-2630022 (Clockwise)

Preceding gene: 187736632

Following gene: 187736634

Centisome position: 98.7

GC content: 57.56

Gene sequence:

>582_bases
ATGGTCACCACGAACGAACCGAATATTTACGAAACGGGAGTACTGGACTCCAATGCGGAAGGCAATTTTGTCTATACCAC
CCTGGACGCCGCCATCAACTGGATTCGTAAAAATTCCCTTTGGCCCATGCCGATGGGTCTTTCCTGCTGCGCCATTGAGT
TCATGGCCGTGGCCTGTTCCCGGTATGACCTTTCCCGCTTCGGCTCGGAAGTGACACGTTTTTCCCCCCGCCAGGCGGAC
GTGATGATTGTAGCCGGCACCGTCACTTATAAAATGGCCCTGGCCGTCCGCCGCATCTGGGACCAGATGCCGGAACCCAA
ATGGTGCATCGCCATGGGCGCGTGCGCTTCCACCGGCGGCATGTTCCGCTCCTATTCCGTACTCCAAGGCGTGGACAAGA
TTCTTCCCGTGGACGTTTACATTTCCGGTTGCCCGCCCCGTCCGGAGGCTATTCTGGAAGCCCTGCTCACCCTGCGCAAG
AAGCTGGATACCCAGCAGCCCGCACGCACTTTCTTCAAGAAGGAAGAACCTCGCGAAGCCAACGCTCCCGTCCCGGTCAA
CACGGAAATGCCCCTTGAATAA

Upstream 100 bases:

>100_bases
GGGCCGGAACAGGCCGCAGAAAATCATGGCTGAACCAAATTAAGGCTTGAGAGTTGAAGGCATCTCGCTAATCTGGCTTC
AGCAAGAATCATCTGTTATC

Downstream 100 bases:

>100_bases
GTTTTTTCAAACGTTTCTTCCGTTCACTTTTACCATTTTTTCTCCATGCTTTCTCTAGAATCACAGATTAAAACCGCCGC
AGACAACGCCCAAAACCGCT

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B

Number of amino acids: Translated: 193; Mature: 193

Protein sequence:

>193_residues
MVTTNEPNIYETGVLDSNAEGNFVYTTLDAAINWIRKNSLWPMPMGLSCCAIEFMAVACSRYDLSRFGSEVTRFSPRQAD
VMIVAGTVTYKMALAVRRIWDQMPEPKWCIAMGACASTGGMFRSYSVLQGVDKILPVDVYISGCPPRPEAILEALLTLRK
KLDTQQPARTFFKKEEPREANAPVPVNTEMPLE

Sequences:

>Translated_193_residues
MVTTNEPNIYETGVLDSNAEGNFVYTTLDAAINWIRKNSLWPMPMGLSCCAIEFMAVACSRYDLSRFGSEVTRFSPRQAD
VMIVAGTVTYKMALAVRRIWDQMPEPKWCIAMGACASTGGMFRSYSVLQGVDKILPVDVYISGCPPRPEAILEALLTLRK
KLDTQQPARTFFKKEEPREANAPVPVNTEMPLE
>Mature_193_residues
MVTTNEPNIYETGVLDSNAEGNFVYTTLDAAINWIRKNSLWPMPMGLSCCAIEFMAVACSRYDLSRFGSEVTRFSPRQAD
VMIVAGTVTYKMALAVRRIWDQMPEPKWCIAMGACASTGGMFRSYSVLQGVDKILPVDVYISGCPPRPEAILEALLTLRK
KLDTQQPARTFFKKEEPREANAPVPVNTEMPLE

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=158, Percent_Identity=50.6329113924051, Blast_Score=180, Evalue=6e-46,
Organism=Escherichia coli, GI1788624, Length=155, Percent_Identity=52.258064516129, Blast_Score=180, Evalue=6e-47,
Organism=Escherichia coli, GI1789074, Length=139, Percent_Identity=42.4460431654676, Blast_Score=103, Evalue=8e-24,
Organism=Escherichia coli, GI1788834, Length=122, Percent_Identity=40.1639344262295, Blast_Score=92, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17509685, Length=164, Percent_Identity=47.5609756097561, Blast_Score=172, Evalue=1e-43,
Organism=Drosophila melanogaster, GI18859983, Length=148, Percent_Identity=52.7027027027027, Blast_Score=175, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24642371, Length=148, Percent_Identity=52.7027027027027, Blast_Score=175, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24651058, Length=160, Percent_Identity=50.625, Blast_Score=175, Evalue=2e-44,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOB_AKKM8 (B2UPT6)

Other databases:

- EMBL:   CP001071
- RefSeq:   YP_001878745.1
- ProteinModelPortal:   B2UPT6
- SMR:   B2UPT6
- GeneID:   6273710
- GenomeReviews:   CP001071_GR
- KEGG:   amu:Amuc_2157
- HOGENOM:   HBG553221
- OMA:   CCAIEFM
- ProtClustDB:   PRK14815
- GO:   GO:0006810
- HAMAP:   MF_01356
- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406
- PANTHER:   PTHR11995:SF2
- PANTHER:   PTHR11995
- TIGRFAMs:   TIGR01957

Pfam domain/function: PF01058 Oxidored_q6

EC number: =1.6.99.5

Molecular weight: Translated: 21537; Mature: 21537

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
8.3 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
8.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTTNEPNIYETGVLDSNAEGNFVYTTLDAAINWIRKNSLWPMPMGLSCCAIEFMAVACS
CCCCCCCCEEECCCCCCCCCCCEEEEEHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
RYDLSRFGSEVTRFSPRQADVMIVAGTVTYKMALAVRRIWDQMPEPKWCIAMGACASTGG
HHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCEEEEECCHHCCCC
MFRSYSVLQGVDKILPVDVYISGCPPRPEAILEALLTLRKKLDTQQPARTFFKKEEPREA
HHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCC
NAPVPVNTEMPLE
CCCCCCCCCCCCC
>Mature Secondary Structure
MVTTNEPNIYETGVLDSNAEGNFVYTTLDAAINWIRKNSLWPMPMGLSCCAIEFMAVACS
CCCCCCCCEEECCCCCCCCCCCEEEEEHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
RYDLSRFGSEVTRFSPRQADVMIVAGTVTYKMALAVRRIWDQMPEPKWCIAMGACASTGG
HHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCEEEEECCHHCCCC
MFRSYSVLQGVDKILPVDVYISGCPPRPEAILEALLTLRKKLDTQQPARTFFKKEEPREA
HHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCC
NAPVPVNTEMPLE
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA