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The map label for this gene is fumB [H]

Identifier: 187736621

GI number: 187736621

Start: 2611159

End: 2612805

Strand: Direct

Name: fumB [H]

Synonym: Amuc_2144

Alternate gene names: 187736621

Gene position: 2611159-2612805 (Clockwise)

Preceding gene: 187736619

Following gene: 187736623

Centisome position: 98.01

GC content: 58.53

Gene sequence:

>1647_bases
ATGGCAACACCGGATTTTCATTATCAGGATCCTTTTCCCATGGAAAAGGACGACACCCAATACCGTCTCATCACGTCAGA
CTATGTGAGCGTAGGCGACTTTGAAGGACACCCCATGCTGAAAGTGGAACCGGAGGGACTGCGCCTGCTGGCAGCGGAAG
CCTTCCATGACGTGGCCTTTTTCCTGCGCCCCGCCCACCTGAAGCAGGTGGCCGCCATTCTGGACGATCCGGAAGCCAGC
GACAATGATAAAAGCGTGGCCCTGACGATGCTCCGCAACGCGGAAGTGGCGTCCGCCGGCATCCTGCCCTTCTGCCAGGA
CACAGGAACCGCCACCATCGTAGGCAAAAAGGGCCAGCAGGTATGGACCGGCTGTGACGACTGCGAACAGCTTTCCCACG
GCGTTTACGACGCCTATACCCAGAACAACCTGCGCTATTCCCAGACGGTGGCGCTGGATATGTACACGGAGAAAAACACC
GGCACCAACCTTCCCGCCCAAATTGACCTGGAAGCGGTGGAGGGCATGAAATACTCCTTCCTGTTCGTCGCCAAGGGCGG
CGGCTCCGCCAACAAAACTTACCTTTACCAGGAAACGAAGGCCCTGCTCAACCCGGCCACGCTGAAAAAGTTCCTGGTGG
AAAAGATGAGTACTCTGGGCACGGCGGCCTGCCCTCCCTATCACATCGCTTTCGTCATTGGCGGAACCTCTGCGGAAAAA
TGCCTGAAGACCGTGAAGATGGCTTCCACGAAGTACTACGACAATCTGCCGACTACGGGGAACGAACACGGCCGTGCCTT
CCGTGACCTGGAACTGGAAAAGGAACTGCATGAAGAAGCGTGCAAACTGGGCCTGGGCGCCCAGTTCGGCGGCAAGTGGT
ACGCTCTGGACGTGCGAGTTATCCGGCTGCCCCGCCACGGCGCCTCCTGCCCGGTTGGCCTGGGAGTCTCCTGCTCTGCG
GACCGCAACGCGAAAGCCAAGATCACGCCGGAGGGCATCTTTATTGAACAGCTGGAAACCAACCCCGGCCAGTATATTCC
GGAAGCCCTGCGCATGACCTCCAGCGAAGCTGTCTCCATTGACCTGAACCGCCCGATGAAGGAAGTTCTGAAAGAACTGG
GCAAGTACCCCGTCACCACGCGCCTGAGCCTGAACGGCACCATCATTGTGGCGCGCGACATCGCCCACGCCAAGCTGAAG
GAACGCCTGGAAAAGGGCGAAGGGCTGCCTCAGTATATGAAGGATCACCCCGTGTACTACGCCGGCCCGGCCAAAACTCC
TGAAGGCTACCCCTCCGGTTCCTTCGGCCCCACGACCGCAGGCCGCATGGATTCCTACGTAGACCTGTTCCAGGAAAACG
GAGGCTCCATGATCATGATTGCCAAGGGCAACCGCAGCCAGCAGGTAACGGATGCCTGCCGGAAGCACGGCGGCTTTTAC
CTGGGCTCCATCGGCGGCCCGGCCGCCATTCTGGCCAAGAACAACATCAAAAAAGTGGAACTGCTGGAATACCCGGAACT
GGGCATGGAAGCCATCTGGAAAATTGAAGTGGAAGATTTCCCCGCCTTCATTCTGGTAGATGACAAGGGCAACGATTTCT
TCGCGAAAATCCGCGAAGGATGGGCCTGCGCCGGTTGCGGGCATTAA

Upstream 100 bases:

>100_bases
TCAAGTATCCGGAACCTTTTCGGGCATGGGCTCCGCATGGGGGCATCCCTGATTTTTTTGCTCGAACCGGATTCGGAGCT
TCGCTATATTGGCTCCCGTT

Downstream 100 bases:

>100_bases
GCCTGAACCGGGAAAAAGATGATTCACTGCCGGAGCCTTTCGGGGCTCCGGCTTTTTTATGCCCCGGGTTCAGCCCTCCC
AGTACAGACGGAACGAATAT

Product: hydro-lyase, Fe-S type, tartrate/fumarate subfamily, beta subunit

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 548; Mature: 547

Protein sequence:

>548_residues
MATPDFHYQDPFPMEKDDTQYRLITSDYVSVGDFEGHPMLKVEPEGLRLLAAEAFHDVAFFLRPAHLKQVAAILDDPEAS
DNDKSVALTMLRNAEVASAGILPFCQDTGTATIVGKKGQQVWTGCDDCEQLSHGVYDAYTQNNLRYSQTVALDMYTEKNT
GTNLPAQIDLEAVEGMKYSFLFVAKGGGSANKTYLYQETKALLNPATLKKFLVEKMSTLGTAACPPYHIAFVIGGTSAEK
CLKTVKMASTKYYDNLPTTGNEHGRAFRDLELEKELHEEACKLGLGAQFGGKWYALDVRVIRLPRHGASCPVGLGVSCSA
DRNAKAKITPEGIFIEQLETNPGQYIPEALRMTSSEAVSIDLNRPMKEVLKELGKYPVTTRLSLNGTIIVARDIAHAKLK
ERLEKGEGLPQYMKDHPVYYAGPAKTPEGYPSGSFGPTTAGRMDSYVDLFQENGGSMIMIAKGNRSQQVTDACRKHGGFY
LGSIGGPAAILAKNNIKKVELLEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFAKIREGWACAGCGH

Sequences:

>Translated_548_residues
MATPDFHYQDPFPMEKDDTQYRLITSDYVSVGDFEGHPMLKVEPEGLRLLAAEAFHDVAFFLRPAHLKQVAAILDDPEAS
DNDKSVALTMLRNAEVASAGILPFCQDTGTATIVGKKGQQVWTGCDDCEQLSHGVYDAYTQNNLRYSQTVALDMYTEKNT
GTNLPAQIDLEAVEGMKYSFLFVAKGGGSANKTYLYQETKALLNPATLKKFLVEKMSTLGTAACPPYHIAFVIGGTSAEK
CLKTVKMASTKYYDNLPTTGNEHGRAFRDLELEKELHEEACKLGLGAQFGGKWYALDVRVIRLPRHGASCPVGLGVSCSA
DRNAKAKITPEGIFIEQLETNPGQYIPEALRMTSSEAVSIDLNRPMKEVLKELGKYPVTTRLSLNGTIIVARDIAHAKLK
ERLEKGEGLPQYMKDHPVYYAGPAKTPEGYPSGSFGPTTAGRMDSYVDLFQENGGSMIMIAKGNRSQQVTDACRKHGGFY
LGSIGGPAAILAKNNIKKVELLEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFAKIREGWACAGCGH
>Mature_547_residues
ATPDFHYQDPFPMEKDDTQYRLITSDYVSVGDFEGHPMLKVEPEGLRLLAAEAFHDVAFFLRPAHLKQVAAILDDPEASD
NDKSVALTMLRNAEVASAGILPFCQDTGTATIVGKKGQQVWTGCDDCEQLSHGVYDAYTQNNLRYSQTVALDMYTEKNTG
TNLPAQIDLEAVEGMKYSFLFVAKGGGSANKTYLYQETKALLNPATLKKFLVEKMSTLGTAACPPYHIAFVIGGTSAEKC
LKTVKMASTKYYDNLPTTGNEHGRAFRDLELEKELHEEACKLGLGAQFGGKWYALDVRVIRLPRHGASCPVGLGVSCSAD
RNAKAKITPEGIFIEQLETNPGQYIPEALRMTSSEAVSIDLNRPMKEVLKELGKYPVTTRLSLNGTIIVARDIAHAKLKE
RLEKGEGLPQYMKDHPVYYAGPAKTPEGYPSGSFGPTTAGRMDSYVDLFQENGGSMIMIAKGNRSQQVTDACRKHGGFYL
GSIGGPAAILAKNNIKKVELLEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFAKIREGWACAGCGH

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1790564, Length=547, Percent_Identity=75.3199268738574, Blast_Score=851, Evalue=0.0,
Organism=Escherichia coli, GI1787897, Length=539, Percent_Identity=74.7680890538034, Blast_Score=843, Evalue=0.0,
Organism=Escherichia coli, GI1789443, Length=185, Percent_Identity=28.1081081081081, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 60103; Mature: 59972

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS00163 FUMARATE_LYASES ; PS00213 LIPOCALIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATPDFHYQDPFPMEKDDTQYRLITSDYVSVGDFEGHPMLKVEPEGLRLLAAEAFHDVAF
CCCCCCCCCCCCCCCCCCCEEEEEEECCEEECCCCCCCEEEECCCCCEEEHHHHHHHHHH
FLRPAHLKQVAAILDDPEASDNDKSVALTMLRNAEVASAGILPFCQDTGTATIVGKKGQQ
HHCHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCHHCCCCCEEECCCCCEEEEECCCCE
VWTGCDDCEQLSHGVYDAYTQNNLRYSQTVALDMYTEKNTGTNLPAQIDLEAVEGMKYSF
EECCCHHHHHHHCCCEEHHHCCCCEEEEEEEEEEEECCCCCCCCCCEEEHHHHCCCEEEE
LFVAKGGGSANKTYLYQETKALLNPATLKKFLVEKMSTLGTAACPPYHIAFVIGGTSAEK
EEEECCCCCCCCEEEEEHHHHHHCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCHHH
CLKTVKMASTKYYDNLPTTGNEHGRAFRDLELEKELHEEACKLGLGAQFGGKWYALDVRV
HHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEE
IRLPRHGASCPVGLGVSCSADRNAKAKITPEGIFIEQLETNPGQYIPEALRMTSSEAVSI
EEECCCCCCCCCCCCEECCCCCCCCEEECCCCEEEEEECCCCCHHHHHHHHCCCCCEEEE
DLNRPMKEVLKELGKYPVTTRLSLNGTIIVARDIAHAKLKERLEKGEGLPQYMKDHPVYY
ECCCHHHHHHHHHCCCCCEEEEECCCEEEEEECHHHHHHHHHHHHCCCCHHHHHCCCEEE
AGPAKTPEGYPSGSFGPTTAGRMDSYVDLFQENGGSMIMIAKGNRSQQVTDACRKHGGFY
ECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCEE
LGSIGGPAAILAKNNIKKVELLEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFAKIREG
EECCCCCEEEEECCCCCEEEEECCCCCCCEEEEEEEECCCCEEEEEECCCCHHHHHHHCC
WACAGCGH
EEECCCCC
>Mature Secondary Structure 
ATPDFHYQDPFPMEKDDTQYRLITSDYVSVGDFEGHPMLKVEPEGLRLLAAEAFHDVAF
CCCCCCCCCCCCCCCCCCEEEEEEECCEEECCCCCCCEEEECCCCCEEEHHHHHHHHHH
FLRPAHLKQVAAILDDPEASDNDKSVALTMLRNAEVASAGILPFCQDTGTATIVGKKGQQ
HHCHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCHHCCCCCEEECCCCCEEEEECCCCE
VWTGCDDCEQLSHGVYDAYTQNNLRYSQTVALDMYTEKNTGTNLPAQIDLEAVEGMKYSF
EECCCHHHHHHHCCCEEHHHCCCCEEEEEEEEEEEECCCCCCCCCCEEEHHHHCCCEEEE
LFVAKGGGSANKTYLYQETKALLNPATLKKFLVEKMSTLGTAACPPYHIAFVIGGTSAEK
EEEECCCCCCCCEEEEEHHHHHHCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCHHH
CLKTVKMASTKYYDNLPTTGNEHGRAFRDLELEKELHEEACKLGLGAQFGGKWYALDVRV
HHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEE
IRLPRHGASCPVGLGVSCSADRNAKAKITPEGIFIEQLETNPGQYIPEALRMTSSEAVSI
EEECCCCCCCCCCCCEECCCCCCCCEEECCCCEEEEEECCCCCHHHHHHHHCCCCCEEEE
DLNRPMKEVLKELGKYPVTTRLSLNGTIIVARDIAHAKLKERLEKGEGLPQYMKDHPVYY
ECCCHHHHHHHHHCCCCCEEEEECCCEEEEEECHHHHHHHHHHHHCCCCHHHHHCCCEEE
AGPAKTPEGYPSGSFGPTTAGRMDSYVDLFQENGGSMIMIAKGNRSQQVTDACRKHGGFY
ECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCEE
LGSIGGPAAILAKNNIKKVELLEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFAKIREG
EECCCCCEEEEECCCCCEEEEECCCCCCCEEEEEEEECCCCEEEEEECCCCHHHHHHHCC
WACAGCGH
EEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]