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The map label for this gene is yafV [C]

Identifier: 187736596

GI number: 187736596

Start: 2580562

End: 2581419

Strand: Reverse

Name: yafV [C]

Synonym: Amuc_2118

Alternate gene names: 187736596

Gene position: 2581419-2580562 (Counterclockwise)

Preceding gene: 187736604

Following gene: 187736595

Centisome position: 96.9

GC content: 59.91

Gene sequence:

>858_bases
ATGCCGAAAATTGCACTCATTCAACTGTCCGCAGATGCCCGGCCTTCCGTGAACAAGGAAAAGACGGAATCCGCCATCCG
TGAAGCCGCCGCCAACGGCGCGCAGATTGTCTGCACCCAGGAACTTTTTACCACGGAATACTTTTGCAGGACGGAGGAAT
GCGATTTATTCGACTTGGCGGAACCTGTTCCCGGGGAATTGACGGAAGCCCACAGGAAACTGGCGGCGGAACTGGGCGTG
GTGATTGTTGCCTCCGGCTTTGAAAAGCGCGCCACCGGGCTTTACCACAATACCGCCTGGGTGGTGGATGCGGACGGTTC
GTTCCTGGGCATGTACCGCAAGATGCATATTCCGCAGGATCCCGGGTTTGAAGAAAAATTCTACTTCACCCCCGGCGACC
TGGGCTATAAGGCGTTCGACACCAAATTCGGCCGCATCGGCGTGCTCATCTGCTGGGACCAGTGGTACCCGGAAGCCGCG
CGCCTGACGGCCATGCAGGGGGCGGAAATCATTTTTTACCCCACGGCCATCGGCTGGCTGCCGGAGGAAAAGCCCCTGCT
GGGGGAACAGCAGCACTGCGCGTGGGAAACCGTGCAGCGCGGCCATGCCGTGGCCAACGGCTGCTATGTCTGCGCCGTCA
ACCGGGTGGGGACGGAAGGGGAAAGCGAATTCTGGGGGCAGTCCTTTGTGGCGGACTACTATGGCCAGATTGTGGCGAAG
GCTCCCGTCAGCGATGAAGCCATCCTGTACGCGGACCTTGACCTGGACGCATTGGAAGACCACCGCCGCATCTGGCCCTT
TTTCCGTGACCGCCGCATTGACTCCTACGGCGGCATCACGGAACGGTGGGGAAAGTAA

Upstream 100 bases:

>100_bases
GCAATTTCATAGCACTTTCCGGGCCGTTTTCCACCAAATAATCGCGCCGCGTTCATTTTCCCTGCTTGGAATGGGAAGGG
AAACGGGGTAAGGAAGGGGC

Downstream 100 bases:

>100_bases
TGTATCCTATATATTTTTATACAAAAATTTGGTATTTGGAAAATACTTGTTGACATTTTTTATTATCTGACCTTTCCTTG
AAAGATATGGATGTGCAATC

Product: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: D-N-alpha-carbamilase [H]

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MPKIALIQLSADARPSVNKEKTESAIREAAANGAQIVCTQELFTTEYFCRTEECDLFDLAEPVPGELTEAHRKLAAELGV
VIVASGFEKRATGLYHNTAWVVDADGSFLGMYRKMHIPQDPGFEEKFYFTPGDLGYKAFDTKFGRIGVLICWDQWYPEAA
RLTAMQGAEIIFYPTAIGWLPEEKPLLGEQQHCAWETVQRGHAVANGCYVCAVNRVGTEGESEFWGQSFVADYYGQIVAK
APVSDEAILYADLDLDALEDHRRIWPFFRDRRIDSYGGITERWGK

Sequences:

>Translated_285_residues
MPKIALIQLSADARPSVNKEKTESAIREAAANGAQIVCTQELFTTEYFCRTEECDLFDLAEPVPGELTEAHRKLAAELGV
VIVASGFEKRATGLYHNTAWVVDADGSFLGMYRKMHIPQDPGFEEKFYFTPGDLGYKAFDTKFGRIGVLICWDQWYPEAA
RLTAMQGAEIIFYPTAIGWLPEEKPLLGEQQHCAWETVQRGHAVANGCYVCAVNRVGTEGESEFWGQSFVADYYGQIVAK
APVSDEAILYADLDLDALEDHRRIWPFFRDRRIDSYGGITERWGK
>Mature_284_residues
PKIALIQLSADARPSVNKEKTESAIREAAANGAQIVCTQELFTTEYFCRTEECDLFDLAEPVPGELTEAHRKLAAELGVV
IVASGFEKRATGLYHNTAWVVDADGSFLGMYRKMHIPQDPGFEEKFYFTPGDLGYKAFDTKFGRIGVLICWDQWYPEAAR
LTAMQGAEIIFYPTAIGWLPEEKPLLGEQQHCAWETVQRGHAVANGCYVCAVNRVGTEGESEFWGQSFVADYYGQIVAKA
PVSDEAILYADLDLDALEDHRRIWPFFRDRRIDSYGGITERWGK

Specific function: The enzyme catalyzes the hydrolysis of N-carbamoyl-D- amino acids to the corresponding which are useful intermediates in the preparation of beta-lactam antibiotics. Industrial production of beta-lactam antibiotics is now being developed using this enzyme

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=281, Percent_Identity=33.8078291814947, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI7706509, Length=277, Percent_Identity=31.7689530685921, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI5031947, Length=290, Percent_Identity=29.3103448275862, Blast_Score=121, Evalue=7e-28,
Organism=Homo sapiens, GI297632350, Length=290, Percent_Identity=29.3103448275862, Blast_Score=121, Evalue=8e-28,
Organism=Homo sapiens, GI297632348, Length=290, Percent_Identity=29.3103448275862, Blast_Score=121, Evalue=8e-28,
Organism=Homo sapiens, GI297632346, Length=188, Percent_Identity=29.2553191489362, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17533173, Length=284, Percent_Identity=32.7464788732394, Blast_Score=127, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI17556280, Length=282, Percent_Identity=26.9503546099291, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6323383, Length=294, Percent_Identity=28.9115646258503, Blast_Score=109, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6322335, Length=311, Percent_Identity=24.1157556270096, Blast_Score=89, Evalue=8e-19,
Organism=Drosophila melanogaster, GI21358471, Length=283, Percent_Identity=32.1554770318021, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI17933642, Length=289, Percent_Identity=25.9515570934256, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI21355835, Length=295, Percent_Identity=26.7796610169492, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.77 [H]

Molecular weight: Translated: 32039; Mature: 31908

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKIALIQLSADARPSVNKEKTESAIREAAANGAQIVCTQELFTTEYFCRTEECDLFDLA
CCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHCCCCCCCHHHCC
EPVPGELTEAHRKLAAELGVVIVASGFEKRATGLYHNTAWVVDADGSFLGMYRKMHIPQD
CCCCCHHHHHHHHHHHHCCEEEEECCCCHHHCCEEECEEEEEECCCCEEEHHHHHCCCCC
PGFEEKFYFTPGDLGYKAFDTKFGRIGVLICWDQWYPEAARLTAMQGAEIIFYPTAIGWL
CCCCCEEEECCCCCCCHHHCCCCCCEEEEEEECCCCCCHHHHHHCCCCEEEEEECEECCC
PEEKPLLGEQQHCAWETVQRGHAVANGCYVCAVNRVGTEGESEFWGQSFVADYYGQIVAK
CCCCCCCCCCHHHHHHHHHCCCHHHCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHEEEE
APVSDEAILYADLDLDALEDHRRIWPFFRDRRIDSYGGITERWGK
CCCCCCEEEEEECCCHHHHHHHHHCCHHHHCCCCCCCCCHHHCCC
>Mature Secondary Structure 
PKIALIQLSADARPSVNKEKTESAIREAAANGAQIVCTQELFTTEYFCRTEECDLFDLA
CCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHCCCCCCCHHHCC
EPVPGELTEAHRKLAAELGVVIVASGFEKRATGLYHNTAWVVDADGSFLGMYRKMHIPQD
CCCCCHHHHHHHHHHHHCCEEEEECCCCHHHCCEEECEEEEEECCCCEEEHHHHHCCCCC
PGFEEKFYFTPGDLGYKAFDTKFGRIGVLICWDQWYPEAARLTAMQGAEIIFYPTAIGWL
CCCCCEEEECCCCCCCHHHCCCCCCEEEEEEECCCCCCHHHHHHCCCCEEEEEECEECCC
PEEKPLLGEQQHCAWETVQRGHAVANGCYVCAVNRVGTEGESEFWGQSFVADYYGQIVAK
CCCCCCCCCCHHHHHHHHHCCCHHHCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHEEEE
APVSDEAILYADLDLDALEDHRRIWPFFRDRRIDSYGGITERWGK
CCCCCCEEEEEECCCHHHHHHHHHCCHHHHCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9648217; 10903946 [H]