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The map label for this gene is gutB [H]

Identifier: 187736594

GI number: 187736594

Start: 2578670

End: 2579791

Strand: Direct

Name: gutB [H]

Synonym: Amuc_2116

Alternate gene names: 187736594

Gene position: 2578670-2579791 (Clockwise)

Preceding gene: 187736591

Following gene: 187736597

Centisome position: 96.79

GC content: 58.38

Gene sequence:

>1122_bases
ATGCAACAAACCGCACGCGCCGCTGTCCTGACAGCACCAAAGACATTTGAAATCCGTGAATATCCCATTCCCGCCATCGG
AGACGATGAAATGCTGATCAAGGTGGAAGCCTGCGGCGTTTGCGGAACGGACGGCCACGAATACAACCGGGACCCCTTCG
GCCTCTGCCCCGTGGTCCTGGGCCATGAAGGCACCGGGGAAATCGTCGCCATGGGCAGGAATATCACTAAAGACACCGCC
GGAAATCCCGTAGCGCTGGGGGACAAGATCGTCACCTGCATCATTCCCTGCGGCACCTGTGACGCCTGCCTGAATACTCC
GGCCCGCACCAACCTGTGCGAAAATGTGGGCGTGTATGGCCTGATGCCTGACGACGACGTGCATCTGAACGGCTACTTCG
GGGAGTACCTCGTCATCCGCAAGGGCTCCACATTTTTCAATGTTTCCGGCATGACGCTGGACCAGCGCATTCTGGTGGAA
CCCGCCGCAGTGGTGGTCCATTCCCTGGAACGCGCCAAGTCCACCGGGCTCCTCAAGTTCAATTCTGTGGTTCTCGTGCA
GGGCTGCGGCCCCATCGGCCTTCTTCAAATCGCCACGCTGCGCACGCTGGGCATTGAAACCATCATCGCTGTGGACGGCA
ATGACTCCCGCCTGGAACTGGCCAGGGAAATGGGAGCCTCCCGCACGTATAACTTCACCCGGTACGCGGATCTGAACGAA
CTGCTGGATGCCGTGAAAAAGGACAACGGCGGCCGCCTGGCGGACTTCGTCTTTCAGTGCACGGGCGTAGGCAAGGCCGG
GGCCAACGCCTGGAAGTTCGTGAAGCGCGGCGGCGGCCTGTGCGAAGTGGGCTTTTTCATGGATGGAGGGGAAAGCGTTA
TCAACCACCATTACGACCTCTGCAACAAGGAGGTAACCGCCGTAGGCTCCTGGGTGTACTCCCCGCAGGACTACCCGACC
ACATTCGACTTTCTGAAGCGAGCCTACGGCATCGGCCTGCCGCTGACCAAGCTGATCTCCCACCGCTTCAAGCTGGATGA
AATCGCGGAAGCCCTGGAAACCAACGTCCAGATGAAAGGCATCAAGATTGCCGTCATTTGTAATTGCAGTAAAAATATAT
AA

Upstream 100 bases:

>100_bases
GACCGGGAAGGAACTTCCCCCCGGTTCCAACAGGCGGAGTTCCTTTCATCACTATTTGTTCTTGAAGAATCCGGGCGGCA
AAGGATAGTGTAAGGGCATC

Downstream 100 bases:

>100_bases
GTAAAAAATAACTCCATAAGATAATCCATGGAAAATATTTTTCTATTTATGTTGCTTTTTCCCTTTATTTAAAAATATCA
AATAAAAAATAACAAGAACC

Product: Alcohol dehydrogenase zinc-binding domain protein

Products: NA

Alternate protein names: Glucitol dehydrogenase; L-iditol 2-dehydrogenase [H]

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MQQTARAAVLTAPKTFEIREYPIPAIGDDEMLIKVEACGVCGTDGHEYNRDPFGLCPVVLGHEGTGEIVAMGRNITKDTA
GNPVALGDKIVTCIIPCGTCDACLNTPARTNLCENVGVYGLMPDDDVHLNGYFGEYLVIRKGSTFFNVSGMTLDQRILVE
PAAVVVHSLERAKSTGLLKFNSVVLVQGCGPIGLLQIATLRTLGIETIIAVDGNDSRLELAREMGASRTYNFTRYADLNE
LLDAVKKDNGGRLADFVFQCTGVGKAGANAWKFVKRGGGLCEVGFFMDGGESVINHHYDLCNKEVTAVGSWVYSPQDYPT
TFDFLKRAYGIGLPLTKLISHRFKLDEIAEALETNVQMKGIKIAVICNCSKNI

Sequences:

>Translated_373_residues
MQQTARAAVLTAPKTFEIREYPIPAIGDDEMLIKVEACGVCGTDGHEYNRDPFGLCPVVLGHEGTGEIVAMGRNITKDTA
GNPVALGDKIVTCIIPCGTCDACLNTPARTNLCENVGVYGLMPDDDVHLNGYFGEYLVIRKGSTFFNVSGMTLDQRILVE
PAAVVVHSLERAKSTGLLKFNSVVLVQGCGPIGLLQIATLRTLGIETIIAVDGNDSRLELAREMGASRTYNFTRYADLNE
LLDAVKKDNGGRLADFVFQCTGVGKAGANAWKFVKRGGGLCEVGFFMDGGESVINHHYDLCNKEVTAVGSWVYSPQDYPT
TFDFLKRAYGIGLPLTKLISHRFKLDEIAEALETNVQMKGIKIAVICNCSKNI
>Mature_373_residues
MQQTARAAVLTAPKTFEIREYPIPAIGDDEMLIKVEACGVCGTDGHEYNRDPFGLCPVVLGHEGTGEIVAMGRNITKDTA
GNPVALGDKIVTCIIPCGTCDACLNTPARTNLCENVGVYGLMPDDDVHLNGYFGEYLVIRKGSTFFNVSGMTLDQRILVE
PAAVVVHSLERAKSTGLLKFNSVVLVQGCGPIGLLQIATLRTLGIETIIAVDGNDSRLELAREMGASRTYNFTRYADLNE
LLDAVKKDNGGRLADFVFQCTGVGKAGANAWKFVKRGGGLCEVGFFMDGGESVINHHYDLCNKEVTAVGSWVYSPQDYPT
TFDFLKRAYGIGLPLTKLISHRFKLDEIAEALETNVQMKGIKIAVICNCSKNI

Specific function: Reduces glucitol to fructose [H]

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI156627571, Length=367, Percent_Identity=25.8855585831063, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI71565154, Length=380, Percent_Identity=28.6842105263158, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI4501939, Length=371, Percent_Identity=26.6846361185984, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI156523966, Length=371, Percent_Identity=26.6846361185984, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI4501929, Length=273, Percent_Identity=28.5714285714286, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI71743840, Length=275, Percent_Identity=25.4545454545455, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI262073058, Length=275, Percent_Identity=25.4545454545455, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI226510992, Length=354, Percent_Identity=30.7909604519774, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1788073, Length=358, Percent_Identity=29.608938547486, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1790045, Length=356, Percent_Identity=27.8089887640449, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1787863, Length=349, Percent_Identity=26.0744985673352, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1788407, Length=341, Percent_Identity=26.099706744868, Blast_Score=104, Evalue=8e-24,
Organism=Escherichia coli, GI1790718, Length=359, Percent_Identity=25.9052924791086, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1786552, Length=379, Percent_Identity=28.7598944591029, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87082125, Length=376, Percent_Identity=24.2021276595745, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI1786825, Length=292, Percent_Identity=26.3698630136986, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17562876, Length=375, Percent_Identity=27.4666666666667, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17562878, Length=371, Percent_Identity=25.8760107816712, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI25146526, Length=374, Percent_Identity=26.7379679144385, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI71997431, Length=374, Percent_Identity=26.2032085561497, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17565904, Length=346, Percent_Identity=26.878612716763, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17562584, Length=319, Percent_Identity=27.2727272727273, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17562582, Length=143, Percent_Identity=34.2657342657343, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71988145, Length=213, Percent_Identity=27.6995305164319, Blast_Score=65, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6322619, Length=375, Percent_Identity=29.6, Blast_Score=140, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6319955, Length=375, Percent_Identity=29.3333333333333, Blast_Score=138, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6323099, Length=355, Percent_Identity=28.169014084507, Blast_Score=120, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6319258, Length=269, Percent_Identity=30.4832713754647, Blast_Score=100, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6320033, Length=367, Percent_Identity=27.5204359673025, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6319257, Length=330, Percent_Identity=26.6666666666667, Blast_Score=94, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6324486, Length=315, Percent_Identity=25.3968253968254, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6323961, Length=315, Percent_Identity=24.4444444444444, Blast_Score=70, Evalue=6e-13,
Organism=Drosophila melanogaster, GI17137530, Length=374, Percent_Identity=28.0748663101604, Blast_Score=118, Evalue=6e-27,
Organism=Drosophila melanogaster, GI17737897, Length=367, Percent_Identity=27.7929155313352, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI17737895, Length=376, Percent_Identity=26.5957446808511, Blast_Score=94, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.14 [H]

Molecular weight: Translated: 40385; Mature: 40385

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQTARAAVLTAPKTFEIREYPIPAIGDDEMLIKVEACGVCGTDGHEYNRDPFGLCPVVL
CCCCCCEEEEECCCEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEE
GHEGTGEIVAMGRNITKDTAGNPVALGDKIVTCIIPCGTCDACLNTPARTNLCENVGVYG
CCCCCCCEEEECCCCCCCCCCCCEEECCEEEEEEEECCCCHHHHCCCHHHHHHHHCCEEE
LMPDDDVHLNGYFGEYLVIRKGSTFFNVSGMTLDQRILVEPAAVVVHSLERAKSTGLLKF
ECCCCCEEEEEEECCEEEEECCCCEEEECCCEECCEEECCHHHHHHHHHHHHHCCCEEEE
NSVVLVQGCGPIGLLQIATLRTLGIETIIAVDGNDSRLELAREMGASRTYNFTRYADLNE
CCEEEEECCCCHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHCCCCCCCCEEEECCHHH
LLDAVKKDNGGRLADFVFQCTGVGKAGANAWKFVKRGGGLCEVGFFMDGGESVINHHYDL
HHHHHHCCCCCCHHHHHEEECCCCCCCCHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHH
CNKEVTAVGSWVYSPQDYPTTFDFLKRAYGIGLPLTKLISHRFKLDEIAEALETNVQMKG
CCCHHHHHEEEEECCCCCCCHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHCCCEECC
IKIAVICNCSKNI
EEEEEEECCCCCC
>Mature Secondary Structure
MQQTARAAVLTAPKTFEIREYPIPAIGDDEMLIKVEACGVCGTDGHEYNRDPFGLCPVVL
CCCCCCEEEEECCCEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEE
GHEGTGEIVAMGRNITKDTAGNPVALGDKIVTCIIPCGTCDACLNTPARTNLCENVGVYG
CCCCCCCEEEECCCCCCCCCCCCEEECCEEEEEEEECCCCHHHHCCCHHHHHHHHCCEEE
LMPDDDVHLNGYFGEYLVIRKGSTFFNVSGMTLDQRILVEPAAVVVHSLERAKSTGLLKF
ECCCCCEEEEEEECCEEEEECCCCEEEECCCEECCEEECCHHHHHHHHHHHHHCCCEEEE
NSVVLVQGCGPIGLLQIATLRTLGIETIIAVDGNDSRLELAREMGASRTYNFTRYADLNE
CCEEEEECCCCHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHCCCCCCCCEEEECCHHH
LLDAVKKDNGGRLADFVFQCTGVGKAGANAWKFVKRGGGLCEVGFFMDGGESVINHHYDL
HHHHHHCCCCCCHHHHHEEECCCCCCCCHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHH
CNKEVTAVGSWVYSPQDYPTTFDFLKRAYGIGLPLTKLISHRFKLDEIAEALETNVQMKG
CCCHHHHHEEEEECCCCCCCHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHCCCEECC
IKIAVICNCSKNI
EEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1460002; 9384377; 8195086 [H]