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The map label for this gene is purF [C]

Identifier: 187734965

GI number: 187734965

Start: 546222

End: 548141

Strand: Direct

Name: purF [C]

Synonym: Amuc_0458

Alternate gene names: 187734965

Gene position: 546222-548141 (Clockwise)

Preceding gene: 187734964

Following gene: 187734966

Centisome position: 20.5

GC content: 55.73

Gene sequence:

>1920_bases
ATGAGCGATTTTCTTAAACACGAGTGCGGCGTAGCCGCCATTCGTCTGCTTAAGCCTCTCTCTTATTTTCAGGATAAATA
CGGCTCCACCCTCTGGGCGTTCAACAAACTGCTCATCCTGATGGAAAAGCAGTACAACCGCGGCCAGGACGGCGCGGGCA
TAGGCTGCGTCAAGCTGAACATGCCCTTGGGGCAGCCTTACCTGTTCCGCACCCGGGACGCCAGCAAAGACGCCCTCACG
AGCATCTTCAACGGGCAAATCAAGAAGCTCAATAAAAAAGTTCGCCGCGGCCAGGTCAATCTGAAGGATGCGGCAGATAT
CAAAAACAACTTCGACTACGGCGGGGAAATCCTGATGGGGCATCTCCGCTACGGCACCTCCGGCCTCTTTGACGAAGGGT
CCTGCCACCCCTACCTGCGCCGCACCAACTGGCCGACCCGTACCCTGATGGTTCTGGGCAACTTCAACATGACCAATACG
CCGGAATTGAACCAGCGCATGATTGAACGCGGCCAGCACCCCGTCTTCGGAACGGACACGCAGACTGTCCTTGAAGAAAT
AGGGTATCACCTGGATGAAGCCCACACGGACCTCTACCGCGCCTTGCGGGACAGCGGCATGCCCGGCCCGGAAATACCCC
ATGCCATTTCCTCCCGGCTCAACATTCAGGAAATTATCCATAACTCCGCCAAGCAATGGGACGGCGGATATGCCATCATG
GGAGCCATCGGTAACGGAGACTACTTCTGCCTGCGGGATCCGCACGGCATCCGCCCATGCCACTACCTGATTACGGATGA
ATTCATTGCCGTGGCCTCCGAACGCGTTCCGCTGATGACCGTTTTTGAAGTGGAAAGCGAACAGGTTCAAGAACTTCCGC
CGGCCAACATGCTCTCTATCAAGGCAGACGGCACACATGCCATTACGGAATTTACCACACCTCTGAAACCGGCTCCCTGC
TCCTTTGAAAAAATTTACTTCTCCCGCGGAAACGATCCTATCGTTTACCGGGAACGCAAAGCGCTCGGTGCGGCATTGAC
CCCCCAAATCGTGGATTCTCTGGAAGACCGTTTTGACAAATCCGCTATCACCTACATTCCCAACACGGCGGAAACCGCCT
ACTACGGCCTACTGGAAGGCCTGCGCGTCTACCGCCGCAAGCGCGTGCATGCCCAGCTTCTGGAAGCCCTGAGAAACGGC
ACCCTGGATGAAAACATGCTGGACAGCGCTATCCTGAAACGCTGGCCGCGCGGTGAAAAAATCGCCCACAAGGATATCAA
AATGCGCACTTTCATCACGCAGGAAAAAAGCCGTGCGCAACTGGTCTCCCATGTATATGACCTTACTTACGGCGCCGTAG
GGCCGGAAGACGTGCTCGTCGCCCTTGACGACTCCATTGTCCGCGGCACCACCTTGAGAAGGTCCATCCTTCGCATCCTG
GGCCGAACCAATCCTCGGAAAATCGTCATTGCATCCACTGCCCCGCAAATCCGGTATCCGGACTGCTATGGCATTGACAT
GTCCGAACTGGGCAACTTCATCGCCTTCCAGGCGGCCGTCTCCCTGATCAAGCAGCACGGACAGGTCCGTCTGCTGGAAG
AAGTATACAAGGCATGCAGGGAAGAACTGGCCAAACCCAAGGAAGAACGGCGCAATTGTGTGAAGGCTATTTACGAAGGG
TTGACGGAGGCTGAAATCTCCAGGGAAATCACCCGTCTGGTCACTCCGCATGACGCTCCGTGCCCCGTGGAAGTCATTTT
CCAGACCATCGAAAACCTCCATGAATCCATAGAAGGCCCCTGCGGCGACTGGTATTTCACCGGAGATTACCCAACTCCGG
GCGGATACACTACAGTTAATGTGGCATACATGCGCTGGTTTGAGGGTAAAGGGGGCCGTGCATACGATTTGCCCTTGTAG

Upstream 100 bases:

>100_bases
AGGCCGCGGCATTCATGTCAAGGTACAGGGCGAATAAAAAACACCATCTCCTTGCAGCGGATACACCACACTTACAGGAC
AATCTTTTCATCATCATCCC

Downstream 100 bases:

>100_bases
CGGCTCCCTTTTTTTGGGTATTCTGTCGACAACATGTCGGGACCCATTTCATACACGACCTTGGAAGACACCGAACTGGT
CGCCAGGGCACGGGAAGGAG

Product: glutamine amidotransferase class-II

Products: L-Glutamate; Diphosphate; 5-Phospho-beta-D-ribosylamine [C]

Alternate protein names: Glutamine Amidotransferase Class-II; Phosphoribosylpyrophosphate Amidotransferase; Class II Glutamine Amidotransferase Domain Protein; Phosphoribosyltransferase; Glutamine Amidotransferase

Number of amino acids: Translated: 639; Mature: 638

Protein sequence:

>639_residues
MSDFLKHECGVAAIRLLKPLSYFQDKYGSTLWAFNKLLILMEKQYNRGQDGAGIGCVKLNMPLGQPYLFRTRDASKDALT
SIFNGQIKKLNKKVRRGQVNLKDAADIKNNFDYGGEILMGHLRYGTSGLFDEGSCHPYLRRTNWPTRTLMVLGNFNMTNT
PELNQRMIERGQHPVFGTDTQTVLEEIGYHLDEAHTDLYRALRDSGMPGPEIPHAISSRLNIQEIIHNSAKQWDGGYAIM
GAIGNGDYFCLRDPHGIRPCHYLITDEFIAVASERVPLMTVFEVESEQVQELPPANMLSIKADGTHAITEFTTPLKPAPC
SFEKIYFSRGNDPIVYRERKALGAALTPQIVDSLEDRFDKSAITYIPNTAETAYYGLLEGLRVYRRKRVHAQLLEALRNG
TLDENMLDSAILKRWPRGEKIAHKDIKMRTFITQEKSRAQLVSHVYDLTYGAVGPEDVLVALDDSIVRGTTLRRSILRIL
GRTNPRKIVIASTAPQIRYPDCYGIDMSELGNFIAFQAAVSLIKQHGQVRLLEEVYKACREELAKPKEERRNCVKAIYEG
LTEAEISREITRLVTPHDAPCPVEVIFQTIENLHESIEGPCGDWYFTGDYPTPGGYTTVNVAYMRWFEGKGGRAYDLPL

Sequences:

>Translated_639_residues
MSDFLKHECGVAAIRLLKPLSYFQDKYGSTLWAFNKLLILMEKQYNRGQDGAGIGCVKLNMPLGQPYLFRTRDASKDALT
SIFNGQIKKLNKKVRRGQVNLKDAADIKNNFDYGGEILMGHLRYGTSGLFDEGSCHPYLRRTNWPTRTLMVLGNFNMTNT
PELNQRMIERGQHPVFGTDTQTVLEEIGYHLDEAHTDLYRALRDSGMPGPEIPHAISSRLNIQEIIHNSAKQWDGGYAIM
GAIGNGDYFCLRDPHGIRPCHYLITDEFIAVASERVPLMTVFEVESEQVQELPPANMLSIKADGTHAITEFTTPLKPAPC
SFEKIYFSRGNDPIVYRERKALGAALTPQIVDSLEDRFDKSAITYIPNTAETAYYGLLEGLRVYRRKRVHAQLLEALRNG
TLDENMLDSAILKRWPRGEKIAHKDIKMRTFITQEKSRAQLVSHVYDLTYGAVGPEDVLVALDDSIVRGTTLRRSILRIL
GRTNPRKIVIASTAPQIRYPDCYGIDMSELGNFIAFQAAVSLIKQHGQVRLLEEVYKACREELAKPKEERRNCVKAIYEG
LTEAEISREITRLVTPHDAPCPVEVIFQTIENLHESIEGPCGDWYFTGDYPTPGGYTTVNVAYMRWFEGKGGRAYDLPL
>Mature_638_residues
SDFLKHECGVAAIRLLKPLSYFQDKYGSTLWAFNKLLILMEKQYNRGQDGAGIGCVKLNMPLGQPYLFRTRDASKDALTS
IFNGQIKKLNKKVRRGQVNLKDAADIKNNFDYGGEILMGHLRYGTSGLFDEGSCHPYLRRTNWPTRTLMVLGNFNMTNTP
ELNQRMIERGQHPVFGTDTQTVLEEIGYHLDEAHTDLYRALRDSGMPGPEIPHAISSRLNIQEIIHNSAKQWDGGYAIMG
AIGNGDYFCLRDPHGIRPCHYLITDEFIAVASERVPLMTVFEVESEQVQELPPANMLSIKADGTHAITEFTTPLKPAPCS
FEKIYFSRGNDPIVYRERKALGAALTPQIVDSLEDRFDKSAITYIPNTAETAYYGLLEGLRVYRRKRVHAQLLEALRNGT
LDENMLDSAILKRWPRGEKIAHKDIKMRTFITQEKSRAQLVSHVYDLTYGAVGPEDVLVALDDSIVRGTTLRRSILRILG
RTNPRKIVIASTAPQIRYPDCYGIDMSELGNFIAFQAAVSLIKQHGQVRLLEEVYKACREELAKPKEERRNCVKAIYEGL
TEAEISREITRLVTPHDAPCPVEVIFQTIENLHESIEGPCGDWYFTGDYPTPGGYTTVNVAYMRWFEGKGGRAYDLPL

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.4.2.14 [C]

Molecular weight: Translated: 72223; Mature: 72091

Theoretical pI: Translated: 7.40; Mature: 7.40

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDFLKHECGVAAIRLLKPLSYFQDKYGSTLWAFNKLLILMEKQYNRGQDGAGIGCVKLN
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEE
MPLGQPYLFRTRDASKDALTSIFNGQIKKLNKKVRRGQVNLKDAADIKNNFDYGGEILMG
CCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCHHEEE
HLRYGTSGLFDEGSCHPYLRRTNWPTRTLMVLGNFNMTNTPELNQRMIERGQHPVFGTDT
EHHCCCCCCCCCCCCCCCHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCH
QTVLEEIGYHLDEAHTDLYRALRDSGMPGPEIPHAISSRLNIQEIIHNSAKQWDGGYAIM
HHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHHCCCCCEEEE
GAIGNGDYFCLRDPHGIRPCHYLITDEFIAVASERVPLMTVFEVESEQVQELPPANMLSI
EEECCCCEEEEECCCCCCCEEHHHHHHHHHHHCCCCCEEEEEECCHHHHHHCCCCCEEEE
KADGTHAITEFTTPLKPAPCSFEKIYFSRGNDPIVYRERKALGAALTPQIVDSLEDRFDK
ECCCCEEHHHCCCCCCCCCCCHHHHEEECCCCCEEEEHHHHHCCCCCHHHHHHHHHHHCC
SAITYIPNTAETAYYGLLEGLRVYRRKRVHAQLLEALRNGTLDENMLDSAILKRWPRGEK
CCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCC
IAHKDIKMRTFITQEKSRAQLVSHVYDLTYGAVGPEDVLVALDDSIVRGTTLRRSILRIL
HHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHH
GRTNPRKIVIASTAPQIRYPDCYGIDMSELGNFIAFQAAVSLIKQHGQVRLLEEVYKACR
CCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
EELAKPKEERRNCVKAIYEGLTEAEISREITRLVTPHDAPCPVEVIFQTIENLHESIEGP
HHHCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCC
CGDWYFTGDYPTPGGYTTVNVAYMRWFEGKGGRAYDLPL
CCCCEEECCCCCCCCCEEEEEEEEEEEECCCCEEECCCC
>Mature Secondary Structure 
SDFLKHECGVAAIRLLKPLSYFQDKYGSTLWAFNKLLILMEKQYNRGQDGAGIGCVKLN
CHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEE
MPLGQPYLFRTRDASKDALTSIFNGQIKKLNKKVRRGQVNLKDAADIKNNFDYGGEILMG
CCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCHHEEE
HLRYGTSGLFDEGSCHPYLRRTNWPTRTLMVLGNFNMTNTPELNQRMIERGQHPVFGTDT
EHHCCCCCCCCCCCCCCCHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCH
QTVLEEIGYHLDEAHTDLYRALRDSGMPGPEIPHAISSRLNIQEIIHNSAKQWDGGYAIM
HHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHHCCCCCEEEE
GAIGNGDYFCLRDPHGIRPCHYLITDEFIAVASERVPLMTVFEVESEQVQELPPANMLSI
EEECCCCEEEEECCCCCCCEEHHHHHHHHHHHCCCCCEEEEEECCHHHHHHCCCCCEEEE
KADGTHAITEFTTPLKPAPCSFEKIYFSRGNDPIVYRERKALGAALTPQIVDSLEDRFDK
ECCCCEEHHHCCCCCCCCCCCHHHHEEECCCCCEEEEHHHHHCCCCCHHHHHHHHHHHCC
SAITYIPNTAETAYYGLLEGLRVYRRKRVHAQLLEALRNGTLDENMLDSAILKRWPRGEK
CCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCC
IAHKDIKMRTFITQEKSRAQLVSHVYDLTYGAVGPEDVLVALDDSIVRGTTLRRSILRIL
HHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHH
GRTNPRKIVIASTAPQIRYPDCYGIDMSELGNFIAFQAAVSLIKQHGQVRLLEEVYKACR
CCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
EELAKPKEERRNCVKAIYEGLTEAEISREITRLVTPHDAPCPVEVIFQTIENLHESIEGP
HHHCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCC
CGDWYFTGDYPTPGGYTTVNVAYMRWFEGKGGRAYDLPL
CCCCEEECCCCCCCCCEEEEEEEEEEEECCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Mg-phosphoribosyldiphosphate Mg-phosphoribosyldiphosphate, [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.067 {5-phospho-alpha-D-ribose} 1.7 {glutamine}} 8.8 {NH3}} [C]

Substrates: L-Glutamine; H2O; 5-Phospho-alpha-D-ribose 1-diphosphate [C]

Specific reaction: L-Glutamine + H2O + 5-Phospho-alpha-D-ribose 1-diphosphate --> L-Glutamate + Diphosphate + 5-Phospho-beta-D-ribosylamine [C]

General reaction: Pentosyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; 2-Mercaptoethanol; 6-Diazo-5-oxo-L-norleucine analogue [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA