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The map label for this gene is algU [H]

Identifier: 187734966

GI number: 187734966

Start: 548175

End: 548774

Strand: Direct

Name: algU [H]

Synonym: Amuc_0459

Alternate gene names: 187734966

Gene position: 548175-548774 (Clockwise)

Preceding gene: 187734965

Following gene: 187734967

Centisome position: 20.58

GC content: 52.17

Gene sequence:

>600_bases
ATGTCGGGACCCATTTCATACACGACCTTGGAAGACACCGAACTGGTCGCCAGGGCACGGGAAGGAGATTCGCGGGCTTT
TGACGAACTTGTCATTCGCCACAGCCGCAAACTCCATGCTACCCTGTACCAGATGACGGACAACTACGACGATGCCTATG
ACATCGCCCAGGAAGCGTTCTCCAAAGCCTACCGGGCCTTGCGCTACTTCAATGGACAAAGCGCCTTTTACACATGGCTG
CACTCCATTGCCGTCAACCACGCCAGAAACTTCCTGAAAAAACGCAACCGCAGAATGACCTACAGTCTGGACGACGACGA
GTACGGCGACCATACGGAAAAGGACATGAACATGGCGGATGAAACGGACGGGGCGGACCCGGAACGCCGTACCCATTTGA
ATGAATTGCAGCTCAAAATCAACGAAGCATTGAAGAAACTTTCCACTAAACACAGGGAAGTCGTTGTTCTTCATGACGTA
AAAGGGCTCAACCATACGGAAATATCCGCCCTTCTCGGTATCTCTGAAGGAACGCTCAGATCACGTCTGCACTATGCCCA
CAAGGAACTCCAGGGGCTGCTGGCGGAATATCTAAGTTAA

Upstream 100 bases:

>100_bases
AGTTAATGTGGCATACATGCGCTGGTTTGAGGGTAAAGGGGGCCGTGCATACGATTTGCCCTTGTAGCGGCTCCCTTTTT
TTGGGTATTCTGTCGACAAC

Downstream 100 bases:

>100_bases
TCAGGAAAAAAAATATTGAACGTCAATTCCCGCCGCCATGTCAAACGCATAGTCCCAAATTCGGGACTGACACATCCTAA
CTTCTATTATACTTAACATC

Product: RNA polymerase, sigma-24 subunit, ECF subfamily

Products: NA

Alternate protein names: Sigma-30 [H]

Number of amino acids: Translated: 199; Mature: 198

Protein sequence:

>199_residues
MSGPISYTTLEDTELVARAREGDSRAFDELVIRHSRKLHATLYQMTDNYDDAYDIAQEAFSKAYRALRYFNGQSAFYTWL
HSIAVNHARNFLKKRNRRMTYSLDDDEYGDHTEKDMNMADETDGADPERRTHLNELQLKINEALKKLSTKHREVVVLHDV
KGLNHTEISALLGISEGTLRSRLHYAHKELQGLLAEYLS

Sequences:

>Translated_199_residues
MSGPISYTTLEDTELVARAREGDSRAFDELVIRHSRKLHATLYQMTDNYDDAYDIAQEAFSKAYRALRYFNGQSAFYTWL
HSIAVNHARNFLKKRNRRMTYSLDDDEYGDHTEKDMNMADETDGADPERRTHLNELQLKINEALKKLSTKHREVVVLHDV
KGLNHTEISALLGISEGTLRSRLHYAHKELQGLLAEYLS
>Mature_198_residues
SGPISYTTLEDTELVARAREGDSRAFDELVIRHSRKLHATLYQMTDNYDDAYDIAQEAFSKAYRALRYFNGQSAFYTWLH
SIAVNHARNFLKKRNRRMTYSLDDDEYGDHTEKDMNMADETDGADPERRTHLNELQLKINEALKKLSTKHREVVVLHDVK
GLNHTEISALLGISEGTLRSRLHYAHKELQGLLAEYLS

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor regulates genes such as algD, involved in alginate biosynthesis [H]

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=181, Percent_Identity=36.4640883977901, Blast_Score=108, Evalue=2e-25,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR014286
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 23019; Mature: 22888

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGPISYTTLEDTELVARAREGDSRAFDELVIRHSRKLHATLYQMTDNYDDAYDIAQEAF
CCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
SKAYRALRYFNGQSAFYTWLHSIAVNHARNFLKKRNRRMTYSLDDDEYGDHTEKDMNMAD
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHCCCCC
ETDGADPERRTHLNELQLKINEALKKLSTKHREVVVLHDVKGLNHTEISALLGISEGTLR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHCCCCHHHH
SRLHYAHKELQGLLAEYLS
HHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SGPISYTTLEDTELVARAREGDSRAFDELVIRHSRKLHATLYQMTDNYDDAYDIAQEAF
CCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
SKAYRALRYFNGQSAFYTWLHSIAVNHARNFLKKRNRRMTYSLDDDEYGDHTEKDMNMAD
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHCCCCC
ETDGADPERRTHLNELQLKINEALKKLSTKHREVVVLHDVKGLNHTEISALLGISEGTLR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHCCCCHHHH
SRLHYAHKELQGLLAEYLS
HHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8432708; 8378309; 7961421; 10984043; 7737518 [H]