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The map label for this gene is bioD

Identifier: 187734906

GI number: 187734906

Start: 481699

End: 482340

Strand: Reverse

Name: bioD

Synonym: Amuc_0398

Alternate gene names: 187734906

Gene position: 482340-481699 (Counterclockwise)

Preceding gene: 187734907

Following gene: 187734905

Centisome position: 18.11

GC content: 53.89

Gene sequence:

>642_bases
ATGAGGAATTTCATTGTTACCGGTACCGATACGGAAGTCGGTAAAACTTATGTGAGCTGCCTGATCGTCAAATCTCTAAG
AGAAGCGGGCATCAATGCCGCCGGGTTCAAACCCGTGGCCTGCGGAGACAGGCAGGATGCCCGCCTTTTGCGCGAAGCCG
GGCCGAAGGATCTGACGCTGGATGAACTGAATCCCGTTTTTTTGAGGAATGCCACGTGTCCTTATGTGGCCGCACGGCTG
GAGAATACGAAAGTGGATGAAAAGGTTATCCTGCGGGCTTATGACGCTTTGTCCGCCGCACATGAGTGCGTGGTGGTGGA
AGGGGTGGGAGGATGGGAAGTCCCGATTGGCCCCGGAAGGAATTTCAGCGATATGGCTGCGGATTTCAAGCTGCCTGTTC
TTCTGGTGATCGGCAACAAGCTGGGAGCCATCAACCATGCCCTGTTAACGCTGAATGCAATCAAGGAACGCGGCCTGGAA
TGCCTGGGCATCGTGTTCAATAATGTGAAAGATGAATGGGATACTGCCTGCGTGACGAACAGGAGCATGGTGGAGGAGTT
TTCCGATGCGCCCATTCTCGGCGAATTGATTCACGGGCAGGATTACATGGACATGGACGTCCTGCTGGAACGTTTGCACT
GA

Upstream 100 bases:

>100_bases
CTGGTCTTTCATCAATCACTGGGTGACCACGAATGACGGCGTGCCTTCCCGCGGGCAGCTTGAGGAGATTAGAAGATCCC
ATCCCTGAATATTTACCGTT

Downstream 100 bases:

>100_bases
CTGGAGGAGTCTGTCTGGCGAATGAACGGGACGATAGCGAAGACGCCCGTTCGTTATTGCCGGATTTGCGGAGGGAAACA
GCGATATGGAGGAATAAGCA

Product: dethiobiotin synthase

Products: NA

Alternate protein names: DTB synthetase; DTBS; Dethiobiotin synthase

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MRNFIVTGTDTEVGKTYVSCLIVKSLREAGINAAGFKPVACGDRQDARLLREAGPKDLTLDELNPVFLRNATCPYVAARL
ENTKVDEKVILRAYDALSAAHECVVVEGVGGWEVPIGPGRNFSDMAADFKLPVLLVIGNKLGAINHALLTLNAIKERGLE
CLGIVFNNVKDEWDTACVTNRSMVEEFSDAPILGELIHGQDYMDMDVLLERLH

Sequences:

>Translated_213_residues
MRNFIVTGTDTEVGKTYVSCLIVKSLREAGINAAGFKPVACGDRQDARLLREAGPKDLTLDELNPVFLRNATCPYVAARL
ENTKVDEKVILRAYDALSAAHECVVVEGVGGWEVPIGPGRNFSDMAADFKLPVLLVIGNKLGAINHALLTLNAIKERGLE
CLGIVFNNVKDEWDTACVTNRSMVEEFSDAPILGELIHGQDYMDMDVLLERLH
>Mature_213_residues
MRNFIVTGTDTEVGKTYVSCLIVKSLREAGINAAGFKPVACGDRQDARLLREAGPKDLTLDELNPVFLRNATCPYVAARL
ENTKVDEKVILRAYDALSAAHECVVVEGVGGWEVPIGPGRNFSDMAADFKLPVLLVIGNKLGAINHALLTLNAIKERGLE
CLGIVFNNVKDEWDTACVTNRSMVEEFSDAPILGELIHGQDYMDMDVLLERLH

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0132

COG function: function code H; Dethiobiotin synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dethiobiotin synthetase family

Homologues:

Organism=Escherichia coli, GI1786995, Length=203, Percent_Identity=34.9753694581281, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI87081944, Length=183, Percent_Identity=34.4262295081967, Blast_Score=99, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOD_AKKM8 (B2UNC5)

Other databases:

- EMBL:   CP001071
- RefSeq:   YP_001877018.1
- ProteinModelPortal:   B2UNC5
- SMR:   B2UNC5
- GeneID:   6274811
- GenomeReviews:   CP001071_GR
- KEGG:   amu:Amuc_0398
- HOGENOM:   HBG650065
- OMA:   INPGLSH
- HAMAP:   MF_00336
- InterPro:   IPR004472
- InterPro:   IPR002586
- PIRSF:   PIRSF006755
- TIGRFAMs:   TIGR00347

Pfam domain/function: PF01656 CbiA

EC number: =6.3.3.3

Molecular weight: Translated: 23328; Mature: 23328

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNFIVTGTDTEVGKTYVSCLIVKSLREAGINAAGFKPVACGDRQDARLLREAGPKDLTL
CCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCH
DELNPVFLRNATCPYVAARLENTKVDEKVILRAYDALSAAHECVVVEGVGGWEVPIGPGR
HHCCCEEEECCCCHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCEECCCCCCC
NFSDMAADFKLPVLLVIGNKLGAINHALLTLNAIKERGLECLGIVFNNVKDEWDTACVTN
CHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCEEHHHHHHCCHHHHHHHHCC
RSMVEEFSDAPILGELIHGQDYMDMDVLLERLH
HHHHHHHCCCCHHHHHHCCCCCCCHHHHHHHCC
>Mature Secondary Structure
MRNFIVTGTDTEVGKTYVSCLIVKSLREAGINAAGFKPVACGDRQDARLLREAGPKDLTL
CCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCH
DELNPVFLRNATCPYVAARLENTKVDEKVILRAYDALSAAHECVVVEGVGGWEVPIGPGR
HHCCCEEEECCCCHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCEECCCCCCC
NFSDMAADFKLPVLLVIGNKLGAINHALLTLNAIKERGLECLGIVFNNVKDEWDTACVTN
CHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCEEHHHHHHCCHHHHHHHHCC
RSMVEEFSDAPILGELIHGQDYMDMDVLLERLH
HHHHHHHCCCCHHHHHHCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA