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The map label for this gene is truA

Identifier: 187734897

GI number: 187734897

Start: 471190

End: 471987

Strand: Direct

Name: truA

Synonym: Amuc_0389

Alternate gene names: 187734897

Gene position: 471190-471987 (Clockwise)

Preceding gene: 187734884

Following gene: 187734900

Centisome position: 17.69

GC content: 59.15

Gene sequence:

>798_bases
ATGACCCGCATTCGTTTCACCGCCGCATATGACGGACGCCCTTATCTGGGCTGGCAAAGCCAGCCGGGAGGCCGTACCGT
CCAGGACGTGCTGGAACGAGCCTTTTCCGGACTCTTTGGAACCACCTGCCGTATCCACGGTTCCGGCAGGACAGACGCCG
GCGTTCATGCTCTGGGCCAGGTTTTTCATGCAGACGCTCCGGATACCCACCGCATTCCTGCGGACAAATGGCCTGCCGCG
CTCAACACGCGCCTTCCCCGAACCATCCGGATTACCCATGCGGAATACGTTCCTCCCGGTTTCCATGCCCGGTTCAGCGC
CACGGGAAAAACCTACCGTTACTGCATTTCACGCGCACCCATCCTCAACCCTTTTGACGCCGGGCTGGCCTGGCACCGCC
CGCTGGCATGGAGCGTGGATATTCTGGAACAGGCGGTACACCTATTCCGGGGAACGCATGACTTCACTGCTTTTGCCGCC
CTGCGCGGCAATGAACCGCGCCCTATTCCGGAGGGTTACTTCCGCCGAACCATCACGCAGACACAAGTGGCACAGACAGG
GGAACATGTCTTCATCACCTTTACCGGAACGGGCTTCCTCTACAAAATGGTGCGCCTCATGACGGGAGCCGCCCATGAAG
CCGCACGTGGAAAAATAACGCTGGAAGAACTGGCTCGCCTCATCAACGCTCCGCTCCCGGATGACAAAAGCCCGTTTTGC
GCCCCGCCGGACGGACTCACGCTGATGAGGGTCCATTACCCGGAAGAAACGTTCGGAGACAAAAAGAAAATAAACTGA

Upstream 100 bases:

>100_bases
AAGCTAAAGAGCGGATGGAACGCACGGCATCCATTCCAAACCGTCACCGTCCGGCACTTGCCGCTTGCCAAAACCGGGCC
GTTCCAACACAATCACCCCT

Downstream 100 bases:

>100_bases
AAACAGCCGTCCTTCTAGACGCGTGCCGTACCGTACAGCCCTTCCCTCCTGATCAGCGCCTCCACTTTCCGGTTCAGATG
GGGAACCGGACATACGCCCG

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 265; Mature: 264

Protein sequence:

>265_residues
MTRIRFTAAYDGRPYLGWQSQPGGRTVQDVLERAFSGLFGTTCRIHGSGRTDAGVHALGQVFHADAPDTHRIPADKWPAA
LNTRLPRTIRITHAEYVPPGFHARFSATGKTYRYCISRAPILNPFDAGLAWHRPLAWSVDILEQAVHLFRGTHDFTAFAA
LRGNEPRPIPEGYFRRTITQTQVAQTGEHVFITFTGTGFLYKMVRLMTGAAHEAARGKITLEELARLINAPLPDDKSPFC
APPDGLTLMRVHYPEETFGDKKKIN

Sequences:

>Translated_265_residues
MTRIRFTAAYDGRPYLGWQSQPGGRTVQDVLERAFSGLFGTTCRIHGSGRTDAGVHALGQVFHADAPDTHRIPADKWPAA
LNTRLPRTIRITHAEYVPPGFHARFSATGKTYRYCISRAPILNPFDAGLAWHRPLAWSVDILEQAVHLFRGTHDFTAFAA
LRGNEPRPIPEGYFRRTITQTQVAQTGEHVFITFTGTGFLYKMVRLMTGAAHEAARGKITLEELARLINAPLPDDKSPFC
APPDGLTLMRVHYPEETFGDKKKIN
>Mature_264_residues
TRIRFTAAYDGRPYLGWQSQPGGRTVQDVLERAFSGLFGTTCRIHGSGRTDAGVHALGQVFHADAPDTHRIPADKWPAAL
NTRLPRTIRITHAEYVPPGFHARFSATGKTYRYCISRAPILNPFDAGLAWHRPLAWSVDILEQAVHLFRGTHDFTAFAAL
RGNEPRPIPEGYFRRTITQTQVAQTGEHVFITFTGTGFLYKMVRLMTGAAHEAARGKITLEELARLINAPLPDDKSPFCA
PPDGLTLMRVHYPEETFGDKKKIN

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=253, Percent_Identity=32.4110671936759, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1788657, Length=259, Percent_Identity=31.2741312741313, Blast_Score=113, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI71985046, Length=242, Percent_Identity=26.8595041322314, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI116007752, Length=304, Percent_Identity=28.6184210526316, Blast_Score=85, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_AKKM8 (B2UNB6)

Other databases:

- EMBL:   CP001071
- RefSeq:   YP_001877009.1
- GeneID:   6274844
- GenomeReviews:   CP001071_GR
- KEGG:   amu:Amuc_0389
- HOGENOM:   HBG621020
- OMA:   QSKTPFR
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 29580; Mature: 29449

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: NA

Important sites: ACT_SITE 52-52

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRIRFTAAYDGRPYLGWQSQPGGRTVQDVLERAFSGLFGTTCRIHGSGRTDAGVHALGQ
CCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHH
VFHADAPDTHRIPADKWPAALNTRLPRTIRITHAEYVPPGFHARFSATGKTYRYCISRAP
HHCCCCCCCCCCCCCCCCHHHHCCCCCEEEEEECCCCCCCCCEEEECCCHHHHHHHHCCC
ILNPFDAGLAWHRPLAWSVDILEQAVHLFRGTHDFTAFAALRGNEPRPIPEGYFRRTITQ
CCCCHHCCCEECCCCEEHHHHHHHHHHHHCCCCCHHEEEEECCCCCCCCCHHHHHHHHHH
TQVAQTGEHVFITFTGTGFLYKMVRLMTGAAHEAARGKITLEELARLINAPLPDDKSPFC
HHHHCCCCEEEEEEECCHHHHHHHHHHHCCHHHHHCCCEEHHHHHHHHCCCCCCCCCCCC
APPDGLTLMRVHYPEETFGDKKKIN
CCCCCCEEEEEECCHHHCCCCCCCC
>Mature Secondary Structure 
TRIRFTAAYDGRPYLGWQSQPGGRTVQDVLERAFSGLFGTTCRIHGSGRTDAGVHALGQ
CEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHH
VFHADAPDTHRIPADKWPAALNTRLPRTIRITHAEYVPPGFHARFSATGKTYRYCISRAP
HHCCCCCCCCCCCCCCCCHHHHCCCCCEEEEEECCCCCCCCCEEEECCCHHHHHHHHCCC
ILNPFDAGLAWHRPLAWSVDILEQAVHLFRGTHDFTAFAALRGNEPRPIPEGYFRRTITQ
CCCCHHCCCEECCCCEEHHHHHHHHHHHHCCCCCHHEEEEECCCCCCCCCHHHHHHHHHH
TQVAQTGEHVFITFTGTGFLYKMVRLMTGAAHEAARGKITLEELARLINAPLPDDKSPFC
HHHHCCCCEEEEEEECCHHHHHHHHHHHCCHHHHHCCCEEHHHHHHHHCCCCCCCCCCCC
APPDGLTLMRVHYPEETFGDKKKIN
CCCCCCEEEEEECCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA