Click here to switch to the map view.

The map label for this gene is thiE [H]

Identifier: 187734884

GI number: 187734884

Start: 450548

End: 451192

Strand: Direct

Name: thiE [H]

Synonym: Amuc_0376

Alternate gene names: 187734884

Gene position: 450548-451192 (Clockwise)

Preceding gene: 187734877

Following gene: 187734897

Centisome position: 16.91

GC content: 53.18

Gene sequence:

>645_bases
ATGAACAGGAAGGAATGCCTCTCATCCTGCCGTCTGTACGGTATTGTAGATATGGGATATACAGCTGAACATCAACTACT
CCCTGTAACAGAAAAACTATTGGCAGGCGGCCTGCGCATTCTCCAGCTAAGGGCAAAAAACCATAACCCGGAACACATTG
AAAATATGGGGCTCCAGCTTGCCCCCCTGTGCCGTAAATACGGCTGTCTTTTCATCATCAACGATTATCCTGAAATTGCC
CTGAACATCGGCGCAGACGGCGTCCATCTGGGACAGGATGACGGAGACTTGGCATCCGTAAGGGGGCTGCTGGGAAAGGA
TGCCGTTATAGGACGGTCCACGCATAGTCCTGAACAGGCCTTGGGTGCATGTGGAGAGCAGGCGGACTATATCGGGTTTG
GGCCCTTGTTCCCCACGGGTACCAAACCGGGAAGACAGGCCATTGGCCTGGAAGACATCGCAAGCGTACAGCAACAGCTA
CCGGAAAATTTCCCTGTCTTCTGTATCGGCGGCATCAATGGGAACACACTGCCCTCCGTACTGGAGGCGGGGGCAAACAG
GGTAGTCATCGTTTCCTGGCTGCTTACACACCCCGACATCACGGGAACAGTCAGAACTCTCAGAAAAGAACTGGGAGAAG
CGTAA

Upstream 100 bases:

>100_bases
AGGCGGGTGACACAATGCGGGGAGTGTATCACACCCTCTTTTAGGGTCAAGAATGGGAAGCGTCTTGCCGGGATGATTTT
ATCCGGTACAATTCCTTGCC

Downstream 100 bases:

>100_bases
CGAAAAACGCACACCCCCAGCCATGTTATCCCTGCCTTTCCTCTTCCTCCGGAGACAGAGCCGGACGGGAGATCAGAAAA
ACGGCGGTATTCACAATCAT

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MNRKECLSSCRLYGIVDMGYTAEHQLLPVTEKLLAGGLRILQLRAKNHNPEHIENMGLQLAPLCRKYGCLFIINDYPEIA
LNIGADGVHLGQDDGDLASVRGLLGKDAVIGRSTHSPEQALGACGEQADYIGFGPLFPTGTKPGRQAIGLEDIASVQQQL
PENFPVFCIGGINGNTLPSVLEAGANRVVIVSWLLTHPDITGTVRTLRKELGEA

Sequences:

>Translated_214_residues
MNRKECLSSCRLYGIVDMGYTAEHQLLPVTEKLLAGGLRILQLRAKNHNPEHIENMGLQLAPLCRKYGCLFIINDYPEIA
LNIGADGVHLGQDDGDLASVRGLLGKDAVIGRSTHSPEQALGACGEQADYIGFGPLFPTGTKPGRQAIGLEDIASVQQQL
PENFPVFCIGGINGNTLPSVLEAGANRVVIVSWLLTHPDITGTVRTLRKELGEA
>Mature_214_residues
MNRKECLSSCRLYGIVDMGYTAEHQLLPVTEKLLAGGLRILQLRAKNHNPEHIENMGLQLAPLCRKYGCLFIINDYPEIA
LNIGADGVHLGQDDGDLASVRGLLGKDAVIGRSTHSPEQALGACGEQADYIGFGPLFPTGTKPGRQAIGLEDIASVQQQL
PENFPVFCIGGINGNTLPSVLEAGANRVVIVSWLLTHPDITGTVRTLRKELGEA

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=163, Percent_Identity=34.9693251533742, Blast_Score=91, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6325042, Length=192, Percent_Identity=31.7708333333333, Blast_Score=95, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 23008; Mature: 23008

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRKECLSSCRLYGIVDMGYTAEHQLLPVTEKLLAGGLRILQLRAKNHNPEHIENMGLQL
CCHHHHHHHHHEEEEEECCCCCCCCCCHHHHHHHHCCEEEEEEECCCCCHHHHHHCCCCH
APLCRKYGCLFIINDYPEIALNIGADGVHLGQDDGDLASVRGLLGKDAVIGRSTHSPEQA
HHHHHHCCEEEEECCCCEEEEEECCCCEECCCCCCHHHHHHHHHCCCCEECCCCCCHHHH
LGACGEQADYIGFGPLFPTGTKPGRQAIGLEDIASVQQQLPENFPVFCIGGINGNTLPSV
HHCCCCCCCCEECCCCCCCCCCCCCHHCCHHHHHHHHHHCCCCCCEEEEECCCCCHHHHH
LEAGANRVVIVSWLLTHPDITGTVRTLRKELGEA
HHCCCCEEEEEEEEHHCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MNRKECLSSCRLYGIVDMGYTAEHQLLPVTEKLLAGGLRILQLRAKNHNPEHIENMGLQL
CCHHHHHHHHHEEEEEECCCCCCCCCCHHHHHHHHCCEEEEEEECCCCCHHHHHHCCCCH
APLCRKYGCLFIINDYPEIALNIGADGVHLGQDDGDLASVRGLLGKDAVIGRSTHSPEQA
HHHHHHCCEEEEECCCCEEEEEECCCCEECCCCCCHHHHHHHHHCCCCEECCCCCCHHHH
LGACGEQADYIGFGPLFPTGTKPGRQAIGLEDIASVQQQLPENFPVFCIGGINGNTLPSV
HHCCCCCCCCEECCCCCCCCCCCCCHHCCHHHHHHHHHHCCCCCCEEEEECCCCCHHHHH
LEAGANRVVIVSWLLTHPDITGTVRTLRKELGEA
HHCCCCEEEEEEEEHHCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA