Click here to switch to the map view.

The map label for this gene is glgP [C]

Identifier: 187734747

GI number: 187734747

Start: 292855

End: 294519

Strand: Direct

Name: glgP [C]

Synonym: Amuc_0235

Alternate gene names: 187734747

Gene position: 292855-294519 (Clockwise)

Preceding gene: 187734746

Following gene: 187734760

Centisome position: 10.99

GC content: 55.56

Gene sequence:

>1665_bases
ATGGCGAAATCATTTTTACCCACCATTTTCGAGCACCCGTATGAAATAGACCCCAAATATGGGAAAAGCGTTGCGTACTT
CTCCATGGAATACGCCATTGATAATTCATTTAAAATCTATTCCGGCGGCCTGGGCTATCTGTCCGGCTCCCATATGCGCA
GTGCGCATGATCTGCGCCAGAACCTCGTAGGCGTCGGCATTCTGTGGAGCTACGGCTACTACAACCAGATCCGTGCGGAG
GACGGATCCATGGCCACGCAATACATGCGTAAAAACTACCCGTTCCTGGAAGATCACAACATCAAATTCCTCATTCATGT
CTGCGGAGCGCCCGTCTGGGTAAAAGCCTACTTCCTGAATCCGGAAACATTCGGCACTGCGCCCATGTTCTTCCTTTCCA
CGGACCTGGAGGAAAATGACGAGGAAAGCCGCAATATCTCCCGCCGCCTGTACGACGCCAACGGCTTCACCCGCATCGCC
CAGTACGTACTGCTGGGCAAAGGAGGCGCCCGCCTTTTTGACGAACTGGGCATTGAACCGGAAATCTACCATCTAAACGA
AGCCCACGGCCTGGCTGCGGCCTTCCACGTGCTTGCCAAGACGGGCAGCGTGGAAGAAGTGCGCAAGCGTTTCGTTTTCA
CTACCCATACCCCGGAAGAAGCCGGCAACGAAAAGATGGACGTAAACACCATGAATACGTTCTCCTTCTTCGACGGGCTC
TCCATGGAACAGGTGCGCGCCGCCGTAGGCATGACGGACAATACGTTCAACTACACGCTGGCCGCCCTGCGCCTCTCCCA
CATCGCCAACGGCGTTTCCAAACTCCATGGAGAAGTATCCCGCCAGATGTGGAAGGACTATGAAGGCATATGCCCCATCA
TCCATATCACGAATGCCCAGAACCAGAAATACTGGCAGGATCCGGAACTGGCGGAAGCTTTCCAGGCCCGCAACAAGGAA
GCGTTCATCCGCCGCAAGCGGGCTCTGAAAAAAGCCCTCTTCCGCATGGTGGGTGAACAAACGGGCCGCGTCTTTGACCC
GAACTGCCTCACCATCGTATGGGCTCGCCGTTTTGCGGCCTACAAGCGTCCGGACCTGGTCACCGGCAATCCCACGATGT
TCGAACGCATGCTCCAGCGCACCAACTATCCGGTGCAGTTCATCTGGGCCGGCAAACCCTACCCGATGGATCATGGCGCT
ATTGAAATCTTCAACCGCCTGAATGACTTGACGGCCAAATATCCCCGTTCCGCCGTGCTCACCGGTTATGAACTGGGCCT
GAGCCGCTACCTGAAAAACGGCTCCGACGTATGGCTCAACAACCCCGTGGTAACCCGGGAAGCATCCGGCACCTCCGGCA
TGTCCGCCGCCATGAACGGCTCCATCTCCGTCTCCACCAATGACGGCTGGATCTGTGAATTCGCCAAGGACGGAGAAAAC
TGCTTCGTCATTCCGGAAGCGCCCGCCCATCTCTCCCCGGAAGCCCGCGACCGCTCCGACCGCGATAACTTCTACAACAT
TCTGGACGACAAGATTCTTCCCCTCTACTACGATCACAACGACAAGTGGATGGACATCGTTTTCAATGCGATGACAGACA
TCTATCCCGAATTCGACTCCGACCGCATGGCGGATCAGTACTACACGGAAATGTACAACAGCTAA

Upstream 100 bases:

>100_bases
ATTCAGACACACACGCAATTTGCCCTTTCCAACAGGAAGGAATCATGATACCAAGGCGTGCGAGTTCTCCTTTTGAACAC
TTTACTCTATTTACAGAACC

Downstream 100 bases:

>100_bases
GATACAGCCTTGTCCCTCTTACGCAACGGCCGCCTTTCTTTGGGAAAGGCGGCCGTTCTTTTTGAAACCGGTGTCAAAAA
TCCACGGGAAGCGGGCCAAA

Product: alpha-glucan phosphorylase

Products: (1,4-alpha-D-glucosyl)n-1; alpha-D-glucose 1-phosphate

Alternate protein names: NA

Number of amino acids: Translated: 554; Mature: 553

Protein sequence:

>554_residues
MAKSFLPTIFEHPYEIDPKYGKSVAYFSMEYAIDNSFKIYSGGLGYLSGSHMRSAHDLRQNLVGVGILWSYGYYNQIRAE
DGSMATQYMRKNYPFLEDHNIKFLIHVCGAPVWVKAYFLNPETFGTAPMFFLSTDLEENDEESRNISRRLYDANGFTRIA
QYVLLGKGGARLFDELGIEPEIYHLNEAHGLAAAFHVLAKTGSVEEVRKRFVFTTHTPEEAGNEKMDVNTMNTFSFFDGL
SMEQVRAAVGMTDNTFNYTLAALRLSHIANGVSKLHGEVSRQMWKDYEGICPIIHITNAQNQKYWQDPELAEAFQARNKE
AFIRRKRALKKALFRMVGEQTGRVFDPNCLTIVWARRFAAYKRPDLVTGNPTMFERMLQRTNYPVQFIWAGKPYPMDHGA
IEIFNRLNDLTAKYPRSAVLTGYELGLSRYLKNGSDVWLNNPVVTREASGTSGMSAAMNGSISVSTNDGWICEFAKDGEN
CFVIPEAPAHLSPEARDRSDRDNFYNILDDKILPLYYDHNDKWMDIVFNAMTDIYPEFDSDRMADQYYTEMYNS

Sequences:

>Translated_554_residues
MAKSFLPTIFEHPYEIDPKYGKSVAYFSMEYAIDNSFKIYSGGLGYLSGSHMRSAHDLRQNLVGVGILWSYGYYNQIRAE
DGSMATQYMRKNYPFLEDHNIKFLIHVCGAPVWVKAYFLNPETFGTAPMFFLSTDLEENDEESRNISRRLYDANGFTRIA
QYVLLGKGGARLFDELGIEPEIYHLNEAHGLAAAFHVLAKTGSVEEVRKRFVFTTHTPEEAGNEKMDVNTMNTFSFFDGL
SMEQVRAAVGMTDNTFNYTLAALRLSHIANGVSKLHGEVSRQMWKDYEGICPIIHITNAQNQKYWQDPELAEAFQARNKE
AFIRRKRALKKALFRMVGEQTGRVFDPNCLTIVWARRFAAYKRPDLVTGNPTMFERMLQRTNYPVQFIWAGKPYPMDHGA
IEIFNRLNDLTAKYPRSAVLTGYELGLSRYLKNGSDVWLNNPVVTREASGTSGMSAAMNGSISVSTNDGWICEFAKDGEN
CFVIPEAPAHLSPEARDRSDRDNFYNILDDKILPLYYDHNDKWMDIVFNAMTDIYPEFDSDRMADQYYTEMYNS
>Mature_553_residues
AKSFLPTIFEHPYEIDPKYGKSVAYFSMEYAIDNSFKIYSGGLGYLSGSHMRSAHDLRQNLVGVGILWSYGYYNQIRAED
GSMATQYMRKNYPFLEDHNIKFLIHVCGAPVWVKAYFLNPETFGTAPMFFLSTDLEENDEESRNISRRLYDANGFTRIAQ
YVLLGKGGARLFDELGIEPEIYHLNEAHGLAAAFHVLAKTGSVEEVRKRFVFTTHTPEEAGNEKMDVNTMNTFSFFDGLS
MEQVRAAVGMTDNTFNYTLAALRLSHIANGVSKLHGEVSRQMWKDYEGICPIIHITNAQNQKYWQDPELAEAFQARNKEA
FIRRKRALKKALFRMVGEQTGRVFDPNCLTIVWARRFAAYKRPDLVTGNPTMFERMLQRTNYPVQFIWAGKPYPMDHGAI
EIFNRLNDLTAKYPRSAVLTGYELGLSRYLKNGSDVWLNNPVVTREASGTSGMSAAMNGSISVSTNDGWICEFAKDGENC
FVIPEAPAHLSPEARDRSDRDNFYNILDDKILPLYYDHNDKWMDIVFNAMTDIYPEFDSDRMADQYYTEMYNS

Specific function: Phosphorylase Is An Important Allosteric Enzyme In Carbohydrate Metabolism. Enzymes From Different Sources Differ In Their Regulatory Mechanisms And In Their Natural Substrates. However, All Known Phosphorylases Share Catalytic And Structural Properties.

COG id: COG0058

COG function: function code G; Glucan phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycogen phosphorylase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011834
- InterPro:   IPR000811 [H]

Pfam domain/function: PF00343 Phosphorylase [H]

EC number: 2.4.1.1

Molecular weight: Translated: 63408; Mature: 63277

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKSFLPTIFEHPYEIDPKYGKSVAYFSMEYAIDNSFKIYSGGLGYLSGSHMRSAHDLRQ
CCCCCCCHHHCCCCCCCCCCCCEEEEEEEEEEECCCEEEEECCCCCCCCCHHHHHHHHHH
NLVGVGILWSYGYYNQIRAEDGSMATQYMRKNYPFLEDHNIKFLIHVCGAPVWVKAYFLN
HHHEEEEEECCCCCCEEECCCCHHHHHHHHHCCCCEECCCCEEEEEECCCCEEEEEEEEC
PETFGTAPMFFLSTDLEENDEESRNISRRLYDANGFTRIAQYVLLGKGGARLFDELGIEP
CCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHCCCC
EIYHLNEAHGLAAAFHVLAKTGSVEEVRKRFVFTTHTPEEAGNEKMDVNTMNTFSFFDGL
EEEECCCHHHHHHHHHHHHCCCCHHHHHHCEEEEECCCHHCCCCEEECCCCCHHHHHCCC
SMEQVRAAVGMTDNTFNYTLAALRLSHIANGVSKLHGEVSRQMWKDYEGICPIIHITNAQ
CHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCC
NQKYWQDPELAEAFQARNKEAFIRRKRALKKALFRMVGEQTGRVFDPNCLTIVWARRFAA
CCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHH
YKRPDLVTGNPTMFERMLQRTNYPVQFIWAGKPYPMDHGAIEIFNRLNDLTAKYPRSAVL
HCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCEE
TGYELGLSRYLKNGSDVWLNNPVVTREASGTSGMSAAMNGSISVSTNDGWICEFAKDGEN
CHHHHHHHHHHCCCCCEEECCCEEEECCCCCCCCHHHCCCEEEEECCCCEEEEEECCCCC
CFVIPEAPAHLSPEARDRSDRDNFYNILDDKILPLYYDHNDKWMDIVFNAMTDIYPEFDS
EEEECCCCCCCCCCCCCCCCCHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCC
DRMADQYYTEMYNS
CHHHHHHHHHHHCC
>Mature Secondary Structure 
AKSFLPTIFEHPYEIDPKYGKSVAYFSMEYAIDNSFKIYSGGLGYLSGSHMRSAHDLRQ
CCCCCCHHHCCCCCCCCCCCCEEEEEEEEEEECCCEEEEECCCCCCCCCHHHHHHHHHH
NLVGVGILWSYGYYNQIRAEDGSMATQYMRKNYPFLEDHNIKFLIHVCGAPVWVKAYFLN
HHHEEEEEECCCCCCEEECCCCHHHHHHHHHCCCCEECCCCEEEEEECCCCEEEEEEEEC
PETFGTAPMFFLSTDLEENDEESRNISRRLYDANGFTRIAQYVLLGKGGARLFDELGIEP
CCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHCCCC
EIYHLNEAHGLAAAFHVLAKTGSVEEVRKRFVFTTHTPEEAGNEKMDVNTMNTFSFFDGL
EEEECCCHHHHHHHHHHHHCCCCHHHHHHCEEEEECCCHHCCCCEEECCCCCHHHHHCCC
SMEQVRAAVGMTDNTFNYTLAALRLSHIANGVSKLHGEVSRQMWKDYEGICPIIHITNAQ
CHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCC
NQKYWQDPELAEAFQARNKEAFIRRKRALKKALFRMVGEQTGRVFDPNCLTIVWARRFAA
CCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHH
YKRPDLVTGNPTMFERMLQRTNYPVQFIWAGKPYPMDHGAIEIFNRLNDLTAKYPRSAVL
HCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCEE
TGYELGLSRYLKNGSDVWLNNPVVTREASGTSGMSAAMNGSISVSTNDGWICEFAKDGEN
CHHHHHHHHHHCCCCCEEECCCEEEECCCCCCCCHHHCCCEEEEECCCCEEEEEECCCCC
CFVIPEAPAHLSPEARDRSDRDNFYNILDDKILPLYYDHNDKWMDIVFNAMTDIYPEFDS
EEEECCCCCCCCCCCCCCCCCHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCC
DRMADQYYTEMYNS
CHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 5'-AMP [C]

Metal ions: CNO- [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 2.7 {glucose} 1 {glucose} [C]

Substrates: (1,4-alpha-D-glucosyl)n; phosphate

Specific reaction: (1,4-alpha-D-glucosyl)n + phosphate = (1,4-alpha-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate

General reaction: Hexosyl group transfer [C]

Inhibitor: ADP glucose; Ag2+; alpha-Amylose; beta-Amylose; Ca2+; CN-; Cu2+; Fe2+; Glucose; Glucose1-phosphate substrate inhibition; Hg2+; IAA; Iodoacetate; K2S2O8; KNO3; NEM; PCMB; TDP glucose; Zn2+ [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]