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The map label for this gene is thiDE [H]

Identifier: 187734735

GI number: 187734735

Start: 279869

End: 280642

Strand: Reverse

Name: thiDE [H]

Synonym: Amuc_0223

Alternate gene names: 187734735

Gene position: 280642-279869 (Counterclockwise)

Preceding gene: 187734737

Following gene: 187734734

Centisome position: 10.53

GC content: 65.63

Gene sequence:

>774_bases
ATGATGACGATTGCCGGTTCCGACTGCTCCGCGGGGGCCGGGCTCCAGGCTGACCTGAAAGCGGCCCATGCCATGGGGGC
GTTTGCGCTTACGGCCGTCACCTGCGTCGTGTCCGAGGCGCCGGGGACTGTGCGCGGCATTCAGGAGGTGGACCCTTCGC
TGGTGGCCGATCAGGTGCGCATCAATCTGGAACATTTTCCCGTGCGCGCCGTGAAGACGGGGATGCTGTATTCCCCGGCT
ATCGTCCGTGCGGTGCATGGGGTGCTGGACGGGGCGGATATTCCCGTCGTGGTGGACCCGGTCATGATCGCCACGGCGGG
CGACCGCCTGATGCGGGAAGAAGCGGTGGCTGTTTATGAAGAGCTTTTGCTGCCGGGTGCGGCATTGCTTACGCCCAATC
TGGATGAGGCTGCCGTATTGCTCCGCTCTTCCGTTAATCCGGGAAGGGACGAACTGCCGGAAGCCGCCGCGCGCCTCGCT
GTCAGGTACGGATGCCCCGTTCTTCTGAAGGGCGGGCATCTGGAAGGGGACTGCCGGGACGTGCTGTCCGGCCCGGACGG
CTGCCTGCTGGGGCAATGGGAGCGTCCGCGCGTACAGGATGTGAGTACTCACGGAACGGGCTGTTCCCTGTCCGCCGCCG
TCGCGGCACGGCTGGCTGCCGGGGATGGACTGGTTGCGGCGGTGGAGCGCGGCCTGGAATTCATCGCTTCCGCAATCCGG
AACCATCTGCGCTGGGAACAGCCGGTCCGGGTGGATGCGCTGAAATTGTGGTAA

Upstream 100 bases:

>100_bases
TTTCCCACCCGGATTTGTGACATTGAGGCGGCACATGCGGATTTTGGGATGTTCAAGGGCTTCTTTCCCGATTAAAAACA
AGGCCATGAGTATTCCCGTA

Downstream 100 bases:

>100_bases
AGCCTCCTTCATCCAAGTTGTTTTCCTCTTCCGCGCGGGTTGGGGTAACCGGGGACTGCCTCCGCCTTTCTGTGCCTTAA
TAACGCTTTGTGCTTGCGTA

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Thiamine-phosphate pyrophosphorylase; TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase; Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MMTIAGSDCSAGAGLQADLKAAHAMGAFALTAVTCVVSEAPGTVRGIQEVDPSLVADQVRINLEHFPVRAVKTGMLYSPA
IVRAVHGVLDGADIPVVVDPVMIATAGDRLMREEAVAVYEELLLPGAALLTPNLDEAAVLLRSSVNPGRDELPEAAARLA
VRYGCPVLLKGGHLEGDCRDVLSGPDGCLLGQWERPRVQDVSTHGTGCSLSAAVAARLAAGDGLVAAVERGLEFIASAIR
NHLRWEQPVRVDALKLW

Sequences:

>Translated_257_residues
MMTIAGSDCSAGAGLQADLKAAHAMGAFALTAVTCVVSEAPGTVRGIQEVDPSLVADQVRINLEHFPVRAVKTGMLYSPA
IVRAVHGVLDGADIPVVVDPVMIATAGDRLMREEAVAVYEELLLPGAALLTPNLDEAAVLLRSSVNPGRDELPEAAARLA
VRYGCPVLLKGGHLEGDCRDVLSGPDGCLLGQWERPRVQDVSTHGTGCSLSAAVAARLAAGDGLVAAVERGLEFIASAIR
NHLRWEQPVRVDALKLW
>Mature_257_residues
MMTIAGSDCSAGAGLQADLKAAHAMGAFALTAVTCVVSEAPGTVRGIQEVDPSLVADQVRINLEHFPVRAVKTGMLYSPA
IVRAVHGVLDGADIPVVVDPVMIATAGDRLMREEAVAVYEELLLPGAALLTPNLDEAAVLLRSSVNPGRDELPEAAARLA
VRYGCPVLLKGGHLEGDCRDVLSGPDGCLLGQWERPRVQDVSTHGTGCSLSAAVAARLAAGDGLVAAVERGLEFIASAIR
NHLRWEQPVRVDALKLW

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=244, Percent_Identity=37.2950819672131, Blast_Score=131, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6325378, Length=250, Percent_Identity=33.2, Blast_Score=113, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6324517, Length=250, Percent_Identity=33.6, Blast_Score=101, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6324997, Length=250, Percent_Identity=33.6, Blast_Score=99, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013749
- InterPro:   IPR004399
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF08543 Phos_pyr_kin; PF02581 TMP-TENI [H]

EC number: =2.5.1.3; =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 26907; Mature: 26907

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTIAGSDCSAGAGLQADLKAAHAMGAFALTAVTCVVSEAPGTVRGIQEVDPSLVADQVR
CEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHE
INLEHFPVRAVKTGMLYSPAIVRAVHGVLDGADIPVVVDPVMIATAGDRLMREEAVAVYE
EEHHHCCHHHHHCCCCCCHHHHHHHHHHHCCCCCCEEECCEEEECCCHHHHHHHHHHHHH
ELLLPGAALLTPNLDEAAVLLRSSVNPGRDELPEAAARLAVRYGCPVLLKGGHLEGDCRD
HHHCCCCCEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHH
VLSGPDGCLLGQWERPRVQDVSTHGTGCSLSAAVAARLAAGDGLVAAVERGLEFIASAIR
HHCCCCCCEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
NHLRWEQPVRVDALKLW
HHHCCCCCCCCEEEECC
>Mature Secondary Structure
MMTIAGSDCSAGAGLQADLKAAHAMGAFALTAVTCVVSEAPGTVRGIQEVDPSLVADQVR
CEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHE
INLEHFPVRAVKTGMLYSPAIVRAVHGVLDGADIPVVVDPVMIATAGDRLMREEAVAVYE
EEHHHCCHHHHHCCCCCCHHHHHHHHHHHCCCCCCEEECCEEEECCCHHHHHHHHHHHHH
ELLLPGAALLTPNLDEAAVLLRSSVNPGRDELPEAAARLAVRYGCPVLLKGGHLEGDCRD
HHHCCCCCEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHH
VLSGPDGCLLGQWERPRVQDVSTHGTGCSLSAAVAARLAAGDGLVAAVERGLEFIASAIR
HHCCCCCCEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
NHLRWEQPVRVDALKLW
HHHCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA