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The map label for this gene is poxB [H]

Identifier: 187734726

GI number: 187734726

Start: 263935

End: 265701

Strand: Reverse

Name: poxB [H]

Synonym: Amuc_0214

Alternate gene names: 187734726

Gene position: 265701-263935 (Counterclockwise)

Preceding gene: 187734727

Following gene: 187734723

Centisome position: 9.97

GC content: 57.95

Gene sequence:

>1767_bases
ATGAATCCGTTGGAGGTTGGCTGGTGTCTAATCTGCATACACAGGGTAGCCGATCAGATTGTTGAAATTTTGGAGAAGGC
GGGAGTTAAGCGGATTTACGGCATTGTGGGGGATAGCCTGAACCCCGTTACGGACGCTTTGAGGAGGAGGAAGACCATTG
AATGGATTCATGTGCGGCATGAGGAAGTGGCGGCTTTTGCCGCGAGCGCGGAAGCCCAGCTGAGCGGGAATCTGGCTGTA
TGCTGCGGCAGCAGCGGCCCCGGGAACCTCCATTTGATCAACGGCCTGTTTGACGCCCACCGGAACCATGCCCCGGTGCT
GGCCATTGCTTCCCACATCCCGCAGAGCCAGGTAGGCATGGAATATTTCCAGGCCACGCATCCGGAACAGCTTTTCAAGG
AATGCAGTGCATATGCGGAACTGGTTTCGGAAGCTTCCCAGGCCCCGTCCATTTTGATGTCCGCCATTCAGCACGCGTGG
GCCAGGCGCGACGTAGGCGTCGTCGTCTTGCCCGGAGACATTGCGGACGCCCCTGCGGAGATGAAGAATCTGGTGCATCC
TCCTTCCTACACGCGGGAGAATGTTGTTCCTGAGCATGAATCCGTATCCCTTCTGGCACGGATGCTTGACGACCACCGCC
GCATTACTTTCTTCTGCGGCGCCGGCTGCGCGGGAGCGGAAGACAAGGTCGTCCGGCTCGCGCACCGCCTGAAAGCTCCG
ATTGCCTACACATGGAGGGGAAAGGATTATTTTGAACATGACAATCCCCTGGGAATCGGCATGACGGGGTTGCTTGGATG
GGGAGATGCCTACAAGGCCATGCATGAAAGCGACATGCTCGTGTTGTGGGGGACGGATTTCCCCTATTTCAATTTCATTC
CCACCAAACCTGAAATCGTGCAGATTGACAGGCGCGGTGAAGTGCTGGGCCGCCGCTGCCGCCTGGACCTGGGTATTTGC
GGGGATGTTTCCGTCACGGCGGAAGCTCTGCTGAACATGGTGCAGGAAAAGACGGATTCCGGGCATTTGGACGCCGCCCT
GAAACGCCACGCCAGGGACGTGAAGGAGATGAACGCCTATATGGAAGGAAATGACAAGGAGTCTCCCATCCGGCCGGAAC
AGCTCACGACCGCCCTGAACAGGCATGCGGCATCCGACGCCGTATTTACGGTAGATACCGGCACCCCCTGTATCTGGAGC
GCCAGGTTCCTGTGCGCCACGCCGGGAAGGAGGACGCTGGCTTCCTTCAATCATGGCTCCATGGCCAATGCCATGCCGAT
GGCTATTGGGGCGCAGATGGAATACCCCGAACGGCAGGTAATTGCCCTGTGCGGAGATGGCGGCCTGTCCATGCTGCTGG
GGGATCTCATCACGATTGTCCAGTACAGGCTGCCGGTGAAAATCGTGGTGTATAACAATGACTGTCTGGATTTCATCCAA
TTGGAAATGCAGGCGGCCGGGCTGATTCCGTGGCAGATCAACCTGAACAACCCCTCTTTTGCCGCCGTGGCGGATGCCGT
CGGCATCAAGGGGTTTTTATTGGACAAATCCAGCCAGGTGGACCAGATGGTGGACATGTTCCTGAATTATCCGGGCGCCG
CTCTGCTGGACGCGCATGTGGACAGGGACGCCCTTGCCCTGCCTCCCTACATCAGTATGGGCCAGGCGGCGGACTTTTCC
CTTGCCATGATGAAGCAGACGCTGGCCGGGGAAGTGAAGCAGGTATGGAACACCCTGGCCGGAAACAGGAAGCTGTTCAA
ACCGTGA

Upstream 100 bases:

>100_bases
CATAGGCCGCTGCCTGAAAAGGCGGCGGCCTTATGATATCAGGGGAAATCCTCAGGCATTTTTTCCGGTGCGGAAAAGAT
GGAGGGGATGACACATAAGA

Downstream 100 bases:

>100_bases
TGCCCTGCGGGCAAGGAGCCGGCATCGGCAAGGGAAGCGGCTCTTTGTTTATGGTCACTCCATGAAAAGGGCTTTTTTCA
GCGCTTCCGTCACGATTTTC

Product: thiamine pyrophosphate protein TPP binding domain protein

Products: NA

Alternate protein names: Pyruvate oxidase; POX [H]

Number of amino acids: Translated: 588; Mature: 588

Protein sequence:

>588_residues
MNPLEVGWCLICIHRVADQIVEILEKAGVKRIYGIVGDSLNPVTDALRRRKTIEWIHVRHEEVAAFAASAEAQLSGNLAV
CCGSSGPGNLHLINGLFDAHRNHAPVLAIASHIPQSQVGMEYFQATHPEQLFKECSAYAELVSEASQAPSILMSAIQHAW
ARRDVGVVVLPGDIADAPAEMKNLVHPPSYTRENVVPEHESVSLLARMLDDHRRITFFCGAGCAGAEDKVVRLAHRLKAP
IAYTWRGKDYFEHDNPLGIGMTGLLGWGDAYKAMHESDMLVLWGTDFPYFNFIPTKPEIVQIDRRGEVLGRRCRLDLGIC
GDVSVTAEALLNMVQEKTDSGHLDAALKRHARDVKEMNAYMEGNDKESPIRPEQLTTALNRHAASDAVFTVDTGTPCIWS
ARFLCATPGRRTLASFNHGSMANAMPMAIGAQMEYPERQVIALCGDGGLSMLLGDLITIVQYRLPVKIVVYNNDCLDFIQ
LEMQAAGLIPWQINLNNPSFAAVADAVGIKGFLLDKSSQVDQMVDMFLNYPGAALLDAHVDRDALALPPYISMGQAADFS
LAMMKQTLAGEVKQVWNTLAGNRKLFKP

Sequences:

>Translated_588_residues
MNPLEVGWCLICIHRVADQIVEILEKAGVKRIYGIVGDSLNPVTDALRRRKTIEWIHVRHEEVAAFAASAEAQLSGNLAV
CCGSSGPGNLHLINGLFDAHRNHAPVLAIASHIPQSQVGMEYFQATHPEQLFKECSAYAELVSEASQAPSILMSAIQHAW
ARRDVGVVVLPGDIADAPAEMKNLVHPPSYTRENVVPEHESVSLLARMLDDHRRITFFCGAGCAGAEDKVVRLAHRLKAP
IAYTWRGKDYFEHDNPLGIGMTGLLGWGDAYKAMHESDMLVLWGTDFPYFNFIPTKPEIVQIDRRGEVLGRRCRLDLGIC
GDVSVTAEALLNMVQEKTDSGHLDAALKRHARDVKEMNAYMEGNDKESPIRPEQLTTALNRHAASDAVFTVDTGTPCIWS
ARFLCATPGRRTLASFNHGSMANAMPMAIGAQMEYPERQVIALCGDGGLSMLLGDLITIVQYRLPVKIVVYNNDCLDFIQ
LEMQAAGLIPWQINLNNPSFAAVADAVGIKGFLLDKSSQVDQMVDMFLNYPGAALLDAHVDRDALALPPYISMGQAADFS
LAMMKQTLAGEVKQVWNTLAGNRKLFKP
>Mature_588_residues
MNPLEVGWCLICIHRVADQIVEILEKAGVKRIYGIVGDSLNPVTDALRRRKTIEWIHVRHEEVAAFAASAEAQLSGNLAV
CCGSSGPGNLHLINGLFDAHRNHAPVLAIASHIPQSQVGMEYFQATHPEQLFKECSAYAELVSEASQAPSILMSAIQHAW
ARRDVGVVVLPGDIADAPAEMKNLVHPPSYTRENVVPEHESVSLLARMLDDHRRITFFCGAGCAGAEDKVVRLAHRLKAP
IAYTWRGKDYFEHDNPLGIGMTGLLGWGDAYKAMHESDMLVLWGTDFPYFNFIPTKPEIVQIDRRGEVLGRRCRLDLGIC
GDVSVTAEALLNMVQEKTDSGHLDAALKRHARDVKEMNAYMEGNDKESPIRPEQLTTALNRHAASDAVFTVDTGTPCIWS
ARFLCATPGRRTLASFNHGSMANAMPMAIGAQMEYPERQVIALCGDGGLSMLLGDLITIVQYRLPVKIVVYNNDCLDFIQ
LEMQAAGLIPWQINLNNPSFAAVADAVGIKGFLLDKSSQVDQMVDMFLNYPGAALLDAHVDRDALALPPYISMGQAADFS
LAMMKQTLAGEVKQVWNTLAGNRKLFKP

Specific function: Unknown

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=545, Percent_Identity=22.5688073394495, Blast_Score=100, Evalue=3e-21,
Organism=Escherichia coli, GI1787096, Length=560, Percent_Identity=49.2857142857143, Blast_Score=533, Evalue=1e-152,
Organism=Escherichia coli, GI1790104, Length=548, Percent_Identity=26.6423357664234, Blast_Score=179, Evalue=7e-46,
Organism=Escherichia coli, GI87081685, Length=521, Percent_Identity=25.1439539347409, Blast_Score=154, Evalue=2e-38,
Organism=Escherichia coli, GI1786717, Length=485, Percent_Identity=26.1855670103093, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1788716, Length=482, Percent_Identity=24.0663900414938, Blast_Score=70, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17531299, Length=476, Percent_Identity=23.7394957983193, Blast_Score=73, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17531301, Length=479, Percent_Identity=23.5908141962422, Blast_Score=72, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6323755, Length=566, Percent_Identity=22.4381625441696, Blast_Score=154, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6320816, Length=522, Percent_Identity=21.0727969348659, Blast_Score=77, Evalue=8e-15,
Organism=Drosophila melanogaster, GI19922626, Length=539, Percent_Identity=22.6345083487941, Blast_Score=107, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =1.2.5.1 [H]

Molecular weight: Translated: 64547; Mature: 64547

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPLEVGWCLICIHRVADQIVEILEKAGVKRIYGIVGDSLNPVTDALRRRKTIEWIHVRH
CCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHCCHHHEEHHH
EEVAAFAASAEAQLSGNLAVCCGSSGPGNLHLINGLFDAHRNHAPVLAIASHIPQSQVGM
HHHHHHHHCCCCEECCCEEEEECCCCCCCEEEEHHHHHHCCCCCCHHHHHHHCCHHHHHH
EYFQATHPEQLFKECSAYAELVSEASQAPSILMSAIQHAWARRDVGVVVLPGDIADAPAE
HHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHH
MKNLVHPPSYTRENVVPEHESVSLLARMLDDHRRITFFCGAGCAGAEDKVVRLAHRLKAP
HHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHCCC
IAYTWRGKDYFEHDNPLGIGMTGLLGWGDAYKAMHESDMLVLWGTDFPYFNFIPTKPEIV
EEEEECCCCCCCCCCCCCCCHHHHCCCCHHHHHHCCCCEEEEECCCCCCCCEECCCCCEE
QIDRRGEVLGRRCRLDLGICGDVSVTAEALLNMVQEKTDSGHLDAALKRHARDVKEMNAY
EECCCHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
MEGNDKESPIRPEQLTTALNRHAASDAVFTVDTGTPCIWSARFLCATPGRRTLASFNHGS
HCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEECCCCHHHHCCCCCC
MANAMPMAIGAQMEYPERQVIALCGDGGLSMLLGDLITIVQYRLPVKIVVYNNDCLDFIQ
CCCCCHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHH
LEMQAAGLIPWQINLNNPSFAAVADAVGIKGFLLDKSSQVDQMVDMFLNYPGAALLDAHV
HHHHHCCCCEEEEECCCCCHHHHHHHHCCCEEEECCCCHHHHHHHHHHCCCCHHHHHHHC
DRDALALPPYISMGQAADFSLAMMKQTLAGEVKQVWNTLAGNRKLFKP
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MNPLEVGWCLICIHRVADQIVEILEKAGVKRIYGIVGDSLNPVTDALRRRKTIEWIHVRH
CCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHCCHHHEEHHH
EEVAAFAASAEAQLSGNLAVCCGSSGPGNLHLINGLFDAHRNHAPVLAIASHIPQSQVGM
HHHHHHHHCCCCEECCCEEEEECCCCCCCEEEEHHHHHHCCCCCCHHHHHHHCCHHHHHH
EYFQATHPEQLFKECSAYAELVSEASQAPSILMSAIQHAWARRDVGVVVLPGDIADAPAE
HHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHH
MKNLVHPPSYTRENVVPEHESVSLLARMLDDHRRITFFCGAGCAGAEDKVVRLAHRLKAP
HHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHCCC
IAYTWRGKDYFEHDNPLGIGMTGLLGWGDAYKAMHESDMLVLWGTDFPYFNFIPTKPEIV
EEEEECCCCCCCCCCCCCCCHHHHCCCCHHHHHHCCCCEEEEECCCCCCCCEECCCCCEE
QIDRRGEVLGRRCRLDLGICGDVSVTAEALLNMVQEKTDSGHLDAALKRHARDVKEMNAY
EECCCHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
MEGNDKESPIRPEQLTTALNRHAASDAVFTVDTGTPCIWSARFLCATPGRRTLASFNHGS
HCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEECCCCHHHHCCCCCC
MANAMPMAIGAQMEYPERQVIALCGDGGLSMLLGDLITIVQYRLPVKIVVYNNDCLDFIQ
CCCCCHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHH
LEMQAAGLIPWQINLNNPSFAAVADAVGIKGFLLDKSSQVDQMVDMFLNYPGAALLDAHV
HHHHHCCCCEEEEECCCCCHHHHHHHHCCCEEEECCCCHHHHHHHHHHCCCCHHHHHHHC
DRDALALPPYISMGQAADFSLAMMKQTLAGEVKQVWNTLAGNRKLFKP
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3016647; 8905232; 9278503; 2663858; 3902830; 8022274 [H]