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The map label for this gene is pfkA [H]

Identifier: 187734723

GI number: 187734723

Start: 260241

End: 261203

Strand: Reverse

Name: pfkA [H]

Synonym: Amuc_0210

Alternate gene names: 187734723

Gene position: 261203-260241 (Counterclockwise)

Preceding gene: 187734726

Following gene: 187734722

Centisome position: 9.8

GC content: 57.63

Gene sequence:

>963_bases
ATGAGTGGAATAGCCATTATGACATCTGGCGGCGATGCCGCCGGAATGAACCCGGCGATCAAGGCGGCAGTGGATCATGC
CCGCCAGCTTGGGATGAACCCGTACGTTGTTTATGACGGCTTGGAAGGGCTGATCGATGGAAAAATCAAGGAAGCCACCC
GGGAACTGACCAACGATATGATCTACAGGGGCGGAACTCTCCTCCGTTCCTCACGATCCAAGCGCTTTTTCGACTACGAA
TTCCGCAAGCAGGCCTACGAACATCTGAAGAAACGCAACATCGACAAGCTCATCATCATGGGCGGGGACGGTTCCTTCCG
CGCCCTGAACGACTTTTTCAATGATTTTGAAGTGCCTTTTGCCGGTATTCCGGCCACGATTGACAACGACATTGCCGGCA
CGGACTACTGCCTGGGCGTGGATACGGCCCAGAACGTGATTCTGGATTGCATTGATTCCGTTCGTGATACGGCCCGTTCC
CATCACCGCGCTTTCGTGATTGAAACGATGGGCCGGGACTGCGGCTATCTGGCGATGACCACCGCTCTTTGCAGCGCGGC
GGATATCTGCATTGTCCCTGAAATTCCGTATGACCTGGAATCCATTTACAAGCGACTCCAGCCTGTCATCAATGAGGAAG
GAAGCTGCATCATTGCCGTGGTGGCGGAAGGCACCCGCGTGGCGCAGTACCTGACCCGCTGGCTGACGGAACGCGCCGGC
ATGGATACCCGCCTGACCGTGCTGGGCCATGTGCAGCGCGGCGGTTCCCCCACCGCCCGTGACCGCCTGATGGGCACCCG
TTTTGCCACCCGCGCCGTGGAAGCTCTGAACAACGGGGACATCAACCAGATCATGGTGTATCATGACGGCCAGTACGGCT
ATGCCTCCCTGGATTCCGTAGCCGGGCAGCAGTACTCCGTGAACCAGGATATCATCAACCTTTGCCGGGAGCTGAGCTGC
TAG

Upstream 100 bases:

>100_bases
CATGAAGCGTTGCCGTTTATTGCGCCAGTAAAAACTCCGCGCTTGCATTATTTGTTCCGCCCGGTAACATTTCCCCCCGG
ACAAGTTAACCTTATTACTT

Downstream 100 bases:

>100_bases
CCGTCTCAGCGGTTACGGAACCCGCTTTTTCCCGCCCATGCCGCCACGTTCCCGCGGCATGGGCGGATTTGTTTTGAAAA
AAAACGCCGCTGCTGTATGA

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MSGIAIMTSGGDAAGMNPAIKAAVDHARQLGMNPYVVYDGLEGLIDGKIKEATRELTNDMIYRGGTLLRSSRSKRFFDYE
FRKQAYEHLKKRNIDKLIIMGGDGSFRALNDFFNDFEVPFAGIPATIDNDIAGTDYCLGVDTAQNVILDCIDSVRDTARS
HHRAFVIETMGRDCGYLAMTTALCSAADICIVPEIPYDLESIYKRLQPVINEEGSCIIAVVAEGTRVAQYLTRWLTERAG
MDTRLTVLGHVQRGGSPTARDRLMGTRFATRAVEALNNGDINQIMVYHDGQYGYASLDSVAGQQYSVNQDIINLCRELSC

Sequences:

>Translated_320_residues
MSGIAIMTSGGDAAGMNPAIKAAVDHARQLGMNPYVVYDGLEGLIDGKIKEATRELTNDMIYRGGTLLRSSRSKRFFDYE
FRKQAYEHLKKRNIDKLIIMGGDGSFRALNDFFNDFEVPFAGIPATIDNDIAGTDYCLGVDTAQNVILDCIDSVRDTARS
HHRAFVIETMGRDCGYLAMTTALCSAADICIVPEIPYDLESIYKRLQPVINEEGSCIIAVVAEGTRVAQYLTRWLTERAG
MDTRLTVLGHVQRGGSPTARDRLMGTRFATRAVEALNNGDINQIMVYHDGQYGYASLDSVAGQQYSVNQDIINLCRELSC
>Mature_319_residues
SGIAIMTSGGDAAGMNPAIKAAVDHARQLGMNPYVVYDGLEGLIDGKIKEATRELTNDMIYRGGTLLRSSRSKRFFDYEF
RKQAYEHLKKRNIDKLIIMGGDGSFRALNDFFNDFEVPFAGIPATIDNDIAGTDYCLGVDTAQNVILDCIDSVRDTARSH
HRAFVIETMGRDCGYLAMTTALCSAADICIVPEIPYDLESIYKRLQPVINEEGSCIIAVVAEGTRVAQYLTRWLTERAGM
DTRLTVLGHVQRGGSPTARDRLMGTRFATRAVEALNNGDINQIMVYHDGQYGYASLDSVAGQQYSVNQDIINLCRELSC

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI266453619, Length=310, Percent_Identity=39.6774193548387, Blast_Score=203, Evalue=1e-52,
Organism=Homo sapiens, GI266453768, Length=310, Percent_Identity=39.6774193548387, Blast_Score=203, Evalue=1e-52,
Organism=Homo sapiens, GI266453748, Length=310, Percent_Identity=39.6774193548387, Blast_Score=203, Evalue=1e-52,
Organism=Homo sapiens, GI4505749, Length=310, Percent_Identity=39.6774193548387, Blast_Score=203, Evalue=1e-52,
Organism=Homo sapiens, GI48762920, Length=307, Percent_Identity=36.8078175895765, Blast_Score=194, Evalue=8e-50,
Organism=Homo sapiens, GI11321601, Length=307, Percent_Identity=35.5048859934853, Blast_Score=185, Evalue=5e-47,
Organism=Escherichia coli, GI1790350, Length=322, Percent_Identity=36.0248447204969, Blast_Score=206, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI25147584, Length=313, Percent_Identity=40.2555910543131, Blast_Score=205, Evalue=3e-53,
Organism=Caenorhabditis elegans, GI17558788, Length=367, Percent_Identity=30.7901907356948, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI72003601, Length=288, Percent_Identity=32.6388888888889, Blast_Score=139, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6321679, Length=308, Percent_Identity=35.3896103896104, Blast_Score=166, Evalue=5e-42,
Organism=Saccharomyces cerevisiae, GI6323861, Length=311, Percent_Identity=35.6913183279743, Blast_Score=164, Evalue=1e-41,
Organism=Drosophila melanogaster, GI28573326, Length=309, Percent_Identity=35.9223300970874, Blast_Score=191, Evalue=5e-49,
Organism=Drosophila melanogaster, GI24652337, Length=310, Percent_Identity=36.4516129032258, Blast_Score=190, Evalue=1e-48,
Organism=Drosophila melanogaster, GI17647809, Length=310, Percent_Identity=36.4516129032258, Blast_Score=189, Evalue=2e-48,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 35310; Mature: 35178

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGIAIMTSGGDAAGMNPAIKAAVDHARQLGMNPYVVYDGLEGLIDGKIKEATRELTNDM
CCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCHHHHCCHHHHHHHHHHHHH
IYRGGTLLRSSRSKRFFDYEFRKQAYEHLKKRNIDKLIIMGGDGSFRALNDFFNDFEVPF
HHCCCHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCC
AGIPATIDNDIAGTDYCLGVDTAQNVILDCIDSVRDTARSHHRAFVIETMGRDCGYLAMT
CCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCHHHHH
TALCSAADICIVPEIPYDLESIYKRLQPVINEEGSCIIAVVAEGTRVAQYLTRWLTERAG
HHHHCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHCC
MDTRLTVLGHVQRGGSPTARDRLMGTRFATRAVEALNNGDINQIMVYHDGQYGYASLDSV
CCEEEHEEEHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHH
AGQQYSVNQDIINLCRELSC
CCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
SGIAIMTSGGDAAGMNPAIKAAVDHARQLGMNPYVVYDGLEGLIDGKIKEATRELTNDM
CCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCHHHHCCHHHHHHHHHHHHH
IYRGGTLLRSSRSKRFFDYEFRKQAYEHLKKRNIDKLIIMGGDGSFRALNDFFNDFEVPF
HHCCCHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCC
AGIPATIDNDIAGTDYCLGVDTAQNVILDCIDSVRDTARSHHRAFVIETMGRDCGYLAMT
CCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCHHHHH
TALCSAADICIVPEIPYDLESIYKRLQPVINEEGSCIIAVVAEGTRVAQYLTRWLTERAG
HHHHCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHCC
MDTRLTVLGHVQRGGSPTARDRLMGTRFATRAVEALNNGDINQIMVYHDGQYGYASLDSV
CCEEEHEEEHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHH
AGQQYSVNQDIINLCRELSC
CCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500908 [H]