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The map label for this gene is gatB [H]

Identifier: 187734725

GI number: 187734725

Start: 262414

End: 263880

Strand: Direct

Name: gatB [H]

Synonym: Amuc_0213

Alternate gene names: 187734725

Gene position: 262414-263880 (Clockwise)

Preceding gene: 187734724

Following gene: 187734728

Centisome position: 9.85

GC content: 57.19

Gene sequence:

>1467_bases
ATGGCAATTTCAGATTACATCGTGACCATTGGCCTGGAAGTCCACTGCCAGATCAAGACAAAGAGCAAAATGTTCTGCTC
CTGCGAGACCGGCTTTGGCTTTGAACCCAATACGCGCGTGTGCCCCACCTGCCTGGGGCTGCCGGGCGCCCTGCCCGTCC
TGAATGAGTTCGCCATTGAACGCACGCTGCTGACCGGCCTGATGCTGGGATGCTCCAGCCCGGAGATTTCCCAATGGGAC
CGCAAAAATTATTTCTATCCGGACATGGCTAAAAATTACCAGACCTCCCAGCTGGATCTTCCGCTGTGCCTGGGAGGGGA
AGTGCCCCTGTACCCCTGGTCCTATCCGAATGACGTGATCAAAGCCGGCATCCCCCCTTTCCGCACCGTGAAGCTGACCC
GCATCCATCTGGAAGAAGACGCGGCGAAGATTACCCATCATGCCAATTATTCCCTGATCGACTACAACCGCGCGGGATCC
GCCCTGATGGAAATCGTTTCCGAACCGGACATCGACACCCCGGAAGAAGCCTACGCCTACCTCAAGTCCCTCCAGCAGAT
TTTGAATTACGGGGATATCTCCGACGCGGACATGGAAAAGGGCCAGATGCGGTGCGATGTGAATATTTCCCTGCGTCCCC
ACGGGCAGAAGGAATTGGGCGCCAAGGTGGAAATGAAGAACATCAACTCCATGTCCGCCGTCCGCAGAGCCCTTCATTAT
GAAATCCAGCGCCAGGCGGAAGAACTGGACATGGGCATTCCTCAAATCCAGTCCACCCGCCGCTGGGACGACGAACGCGG
AGAAAGCGTCGTCATGCGAACCAAGGAAGACGCGCACGATTACCGTTATTTCCCCTGTCCGGATCTGGTTCCCGTACGCA
CGGCCCCGCTGGTGGAGAAAGTGCGCGGGCAGATTCCCGAACTGCCTCAGGAACGGCAGAAACGCTTCATGGAAGAATAC
GGCCTGAGCGAGTACGACGCCAACGTGCTGGTGGGAGACCTGGAACTGGCACGCTTTTTTGAAGAGGCGGCGCGGGGAAC
CGCCAGCGGCAAAAAAATCGCCAACTGGGTGATTAACAACATTTCCGCCGTGCTGAATGAAAAAGGCATGCGCCCGTCTG
AATGCCCGGTGAAACCCGGCGCCATCCGGGAGCTGATCGCCATTATTGACGACGGCACCGTTTCCAACAACCAGGCCAAG
GACGTATTCGCCAAAATGTGGGATGAGCCGGCCCTCGCCGCAGCGCAGGCCGCCAAGCTGCTGGGCTTTGAAAAGGCGGA
TTCCTCTTTTCTGGACGGCATCGTGCGGGAAGTCATTTCCGCCAATCCGGACAAGGTGGCGGAAATACAGGGGGGCAATG
AAAAGCTCCTGAACTGGCTTACCGGCCAGGTCATGAAGGCCGCCAAAGGCAAAGCCAACCCGAAAATCGTGACGGAAGCG
CTGAAAAAAGCCCTTTTCATGGAGTGA

Upstream 100 bases:

>100_bases
CTTAGACTGAAAACCCTCCGCTCTCCCCCGCAGGCCTTTTTACTGGACAGGGGGAAAAGGGTTCCGTACAGTTTCCGCGA
TCTTCAAGCGTTCCGAATTT

Downstream 100 bases:

>100_bases
CCATAAACAAAGAGCCGCTTCCCTTGCCGATGCCGGCTCCTTGCCCGCAGGGCATCACGGTTTGAACAGCTTCCTGTTTC
CGGCCAGGGTGTTCCATACC

Product: glutamyl-tRNA(Gln) amidotransferase, B subunit

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 488; Mature: 487

Protein sequence:

>488_residues
MAISDYIVTIGLEVHCQIKTKSKMFCSCETGFGFEPNTRVCPTCLGLPGALPVLNEFAIERTLLTGLMLGCSSPEISQWD
RKNYFYPDMAKNYQTSQLDLPLCLGGEVPLYPWSYPNDVIKAGIPPFRTVKLTRIHLEEDAAKITHHANYSLIDYNRAGS
ALMEIVSEPDIDTPEEAYAYLKSLQQILNYGDISDADMEKGQMRCDVNISLRPHGQKELGAKVEMKNINSMSAVRRALHY
EIQRQAEELDMGIPQIQSTRRWDDERGESVVMRTKEDAHDYRYFPCPDLVPVRTAPLVEKVRGQIPELPQERQKRFMEEY
GLSEYDANVLVGDLELARFFEEAARGTASGKKIANWVINNISAVLNEKGMRPSECPVKPGAIRELIAIIDDGTVSNNQAK
DVFAKMWDEPALAAAQAAKLLGFEKADSSFLDGIVREVISANPDKVAEIQGGNEKLLNWLTGQVMKAAKGKANPKIVTEA
LKKALFME

Sequences:

>Translated_488_residues
MAISDYIVTIGLEVHCQIKTKSKMFCSCETGFGFEPNTRVCPTCLGLPGALPVLNEFAIERTLLTGLMLGCSSPEISQWD
RKNYFYPDMAKNYQTSQLDLPLCLGGEVPLYPWSYPNDVIKAGIPPFRTVKLTRIHLEEDAAKITHHANYSLIDYNRAGS
ALMEIVSEPDIDTPEEAYAYLKSLQQILNYGDISDADMEKGQMRCDVNISLRPHGQKELGAKVEMKNINSMSAVRRALHY
EIQRQAEELDMGIPQIQSTRRWDDERGESVVMRTKEDAHDYRYFPCPDLVPVRTAPLVEKVRGQIPELPQERQKRFMEEY
GLSEYDANVLVGDLELARFFEEAARGTASGKKIANWVINNISAVLNEKGMRPSECPVKPGAIRELIAIIDDGTVSNNQAK
DVFAKMWDEPALAAAQAAKLLGFEKADSSFLDGIVREVISANPDKVAEIQGGNEKLLNWLTGQVMKAAKGKANPKIVTEA
LKKALFME
>Mature_487_residues
AISDYIVTIGLEVHCQIKTKSKMFCSCETGFGFEPNTRVCPTCLGLPGALPVLNEFAIERTLLTGLMLGCSSPEISQWDR
KNYFYPDMAKNYQTSQLDLPLCLGGEVPLYPWSYPNDVIKAGIPPFRTVKLTRIHLEEDAAKITHHANYSLIDYNRAGSA
LMEIVSEPDIDTPEEAYAYLKSLQQILNYGDISDADMEKGQMRCDVNISLRPHGQKELGAKVEMKNINSMSAVRRALHYE
IQRQAEELDMGIPQIQSTRRWDDERGESVVMRTKEDAHDYRYFPCPDLVPVRTAPLVEKVRGQIPELPQERQKRFMEEYG
LSEYDANVLVGDLELARFFEEAARGTASGKKIANWVINNISAVLNEKGMRPSECPVKPGAIRELIAIIDDGTVSNNQAKD
VFAKMWDEPALAAAQAAKLLGFEKADSSFLDGIVREVISANPDKVAEIQGGNEKLLNWLTGQVMKAAKGKANPKIVTEAL
KKALFME

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=500, Percent_Identity=34.2, Blast_Score=278, Evalue=1e-74,
Organism=Caenorhabditis elegans, GI115534094, Length=286, Percent_Identity=36.7132867132867, Blast_Score=177, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6319391, Length=528, Percent_Identity=27.4621212121212, Blast_Score=187, Evalue=5e-48,
Organism=Drosophila melanogaster, GI45550808, Length=493, Percent_Identity=32.4543610547667, Blast_Score=250, Evalue=2e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 54527; Mature: 54396

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS01186 EGF_2 ; PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAISDYIVTIGLEVHCQIKTKSKMFCSCETGFGFEPNTRVCPTCLGLPGALPVLNEFAIE
CCCCCEEEEEEEEEEEEEEECCCEEEEECCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHH
RTLLTGLMLGCSSPEISQWDRKNYFYPDMAKNYQTSQLDLPLCLGGEVPLYPWSYPNDVI
HHHHHHHHHCCCCCCCCHHHCCCCCCCHHHCCCCCCCCCCCEEECCCCCCCCCCCCHHHH
KAGIPPFRTVKLTRIHLEEDAAKITHHANYSLIDYNRAGSALMEIVSEPDIDTPEEAYAY
HCCCCCCCEEEEEEEEEHHHHHHHHHCCCCEEEECCCHHHHHHHHHCCCCCCCHHHHHHH
LKSLQQILNYGDISDADMEKGQMRCDVNISLRPHGQKELGAKVEMKNINSMSAVRRALHY
HHHHHHHHCCCCCCCCHHHCCCEEEEEEEEECCCCHHHHCCEEEECCCHHHHHHHHHHHH
EIQRQAEELDMGIPQIQSTRRWDDERGESVVMRTKEDAHDYRYFPCPDLVPVRTAPLVEK
HHHHHHHHHHCCCCHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHH
VRGQIPELPQERQKRFMEEYGLSEYDANVLVGDLELARFFEEAARGTASGKKIANWVINN
HHCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHCCCCCCHHHHHHHHHH
ISAVLNEKGMRPSECPVKPGAIRELIAIIDDGTVSNNQAKDVFAKMWDEPALAAAQAAKL
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHH
LGFEKADSSFLDGIVREVISANPDKVAEIQGGNEKLLNWLTGQVMKAAKGKANPKIVTEA
HCCHHCCHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LKKALFME
HHHHHCCC
>Mature Secondary Structure 
AISDYIVTIGLEVHCQIKTKSKMFCSCETGFGFEPNTRVCPTCLGLPGALPVLNEFAIE
CCCCEEEEEEEEEEEEEEECCCEEEEECCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHH
RTLLTGLMLGCSSPEISQWDRKNYFYPDMAKNYQTSQLDLPLCLGGEVPLYPWSYPNDVI
HHHHHHHHHCCCCCCCCHHHCCCCCCCHHHCCCCCCCCCCCEEECCCCCCCCCCCCHHHH
KAGIPPFRTVKLTRIHLEEDAAKITHHANYSLIDYNRAGSALMEIVSEPDIDTPEEAYAY
HCCCCCCCEEEEEEEEEHHHHHHHHHCCCCEEEECCCHHHHHHHHHCCCCCCCHHHHHHH
LKSLQQILNYGDISDADMEKGQMRCDVNISLRPHGQKELGAKVEMKNINSMSAVRRALHY
HHHHHHHHCCCCCCCCHHHCCCEEEEEEEEECCCCHHHHCCEEEECCCHHHHHHHHHHHH
EIQRQAEELDMGIPQIQSTRRWDDERGESVVMRTKEDAHDYRYFPCPDLVPVRTAPLVEK
HHHHHHHHHHCCCCHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHH
VRGQIPELPQERQKRFMEEYGLSEYDANVLVGDLELARFFEEAARGTASGKKIANWVINN
HHCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHCCCCCCHHHHHHHHHH
ISAVLNEKGMRPSECPVKPGAIRELIAIIDDGTVSNNQAKDVFAKMWDEPALAAAQAAKL
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHH
LGFEKADSSFLDGIVREVISANPDKVAEIQGGNEKLLNWLTGQVMKAAKGKANPKIVTEA
HCCHHCCHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LKKALFME
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA