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The map label for this gene is rfbB1 [H]

Identifier: 187734722

GI number: 187734722

Start: 259057

End: 260109

Strand: Reverse

Name: rfbB1 [H]

Synonym: Amuc_0209

Alternate gene names: 187734722

Gene position: 260109-259057 (Counterclockwise)

Preceding gene: 187734723

Following gene: 187734721

Centisome position: 9.76

GC content: 58.4

Gene sequence:

>1053_bases
ATGAAAGTTATCGTTACGGGCGGCGCCGGATTCATCGGCTCCAATCTGGTCAGGCTGCTGCTGGAGCGCGGCCATGAAGT
CTTGAATATTGACGCCCTGACCTATGCCGGCAACATCCATTCACTGGACGGCTCGGCGGATTCTCCGGCATACCGCTTTC
TGCACGCCGACGTCCGGGACGCTTCCCTGATGCGGCGCACTTTGCGGGAGTACGCTCCGGACTGGGTGATGCACCTGGCG
GCGGAATCCCATGTGGACCGTTCCATTGACGATCCGGGAAATTTTATGACCACCAACGTCATGGGCACTTTTTCCATGCT
TCAGGCCGCCCTGGAATATTACCGTTCCCTGGAGGGAGAGAAAAAGGAGCGTTTCCGCTTTCATCACATTTCCACGGATG
AAGTGTACGGCTCCCTGGGAAAGGAAGGCCTGTTCACGGAGTCCACGCCGTATCGCCCCCATTCCCCGTATTCCGCCAGC
AAGGCCTCCAGCGACCACCTGGTGCGCGCCTGGCACGATACGTACGGGCTGCCCGTGCTGATCACCAACTGTTCCAACAA
TTACGGCCCCTACCAGTTCCCGGAAAAGCTGATACCCGTCGTTATCCTGAAAGCGCTGGGCGGGGAACCCATTCCTGTAT
ACGGCAAAGGGGAAAACGTGCGGGACTGGCTGTATGTGGGGGATCACTGCGAGGCGCTGTTGACGGTCGTTTCCTGCGGA
AAACCGGGGGAAACGTACAACATCGGCGGCAATAACGAGAAAACCAATCTGGAGCTGGTGGAGCTGCTGTGTTCCCATCT
GGATGAACTGCGGCCCCGTCCGGACGGCCGGAGCTACCGGGAGCAAATCTCCTTTGTGCAGGACAGGCCGGGCCATGACC
TGCGCTATGCGATTGACGCTTCCAAAATACGGAGGGAATTGGGGTGGACTCCGCGCCAGGACCATTCTTCCGGATTCAGA
AAGACCATTCAGTGGTATCTGGACCATGAGGAATGGTGGCGGGGCATCCTGTCCGGGGAATACCGCCTGGAACGTCTTGG
AACCAATGGCTAG

Upstream 100 bases:

>100_bases
CCGCCCATGCCGCCACGTTCCCGCGGCATGGGCGGATTTGTTTTGAAAAAAAACGCCGCTGCTGTATGATGGCGCCGCAT
CTTTCCCTGAAAACAGTGCT

Downstream 100 bases:

>100_bases
GAATTTGATATGAAGGGTATTGTTCTGGCCGGGGGAAGCGGCACGCGTCTGCATCCCGTTACGCTTTCCGTCAGCAAGCA
ATTGCTGCCCGTTTACGATA

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MKVIVTGGAGFIGSNLVRLLLERGHEVLNIDALTYAGNIHSLDGSADSPAYRFLHADVRDASLMRRTLREYAPDWVMHLA
AESHVDRSIDDPGNFMTTNVMGTFSMLQAALEYYRSLEGEKKERFRFHHISTDEVYGSLGKEGLFTESTPYRPHSPYSAS
KASSDHLVRAWHDTYGLPVLITNCSNNYGPYQFPEKLIPVVILKALGGEPIPVYGKGENVRDWLYVGDHCEALLTVVSCG
KPGETYNIGGNNEKTNLELVELLCSHLDELRPRPDGRSYREQISFVQDRPGHDLRYAIDASKIRRELGWTPRQDHSSGFR
KTIQWYLDHEEWWRGILSGEYRLERLGTNG

Sequences:

>Translated_350_residues
MKVIVTGGAGFIGSNLVRLLLERGHEVLNIDALTYAGNIHSLDGSADSPAYRFLHADVRDASLMRRTLREYAPDWVMHLA
AESHVDRSIDDPGNFMTTNVMGTFSMLQAALEYYRSLEGEKKERFRFHHISTDEVYGSLGKEGLFTESTPYRPHSPYSAS
KASSDHLVRAWHDTYGLPVLITNCSNNYGPYQFPEKLIPVVILKALGGEPIPVYGKGENVRDWLYVGDHCEALLTVVSCG
KPGETYNIGGNNEKTNLELVELLCSHLDELRPRPDGRSYREQISFVQDRPGHDLRYAIDASKIRRELGWTPRQDHSSGFR
KTIQWYLDHEEWWRGILSGEYRLERLGTNG
>Mature_350_residues
MKVIVTGGAGFIGSNLVRLLLERGHEVLNIDALTYAGNIHSLDGSADSPAYRFLHADVRDASLMRRTLREYAPDWVMHLA
AESHVDRSIDDPGNFMTTNVMGTFSMLQAALEYYRSLEGEKKERFRFHHISTDEVYGSLGKEGLFTESTPYRPHSPYSAS
KASSDHLVRAWHDTYGLPVLITNCSNNYGPYQFPEKLIPVVILKALGGEPIPVYGKGENVRDWLYVGDHCEALLTVVSCG
KPGETYNIGGNNEKTNLELVELLCSHLDELRPRPDGRSYREQISFVQDRPGHDLRYAIDASKIRRELGWTPRQDHSSGFR
KTIQWYLDHEEWWRGILSGEYRLERLGTNG

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=338, Percent_Identity=36.6863905325444, Blast_Score=223, Evalue=2e-58,
Organism=Homo sapiens, GI42516563, Length=337, Percent_Identity=24.9258160237389, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI4504031, Length=339, Percent_Identity=23.8938053097345, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI48994969, Length=353, Percent_Identity=60.3399433427762, Blast_Score=437, Evalue=1e-124,
Organism=Escherichia coli, GI1788353, Length=352, Percent_Identity=58.8068181818182, Blast_Score=426, Evalue=1e-120,
Organism=Escherichia coli, GI1788366, Length=339, Percent_Identity=28.023598820059, Blast_Score=101, Evalue=9e-23,
Organism=Escherichia coli, GI1786974, Length=359, Percent_Identity=27.5766016713092, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17568069, Length=336, Percent_Identity=36.6071428571429, Blast_Score=199, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI115532424, Length=329, Percent_Identity=31.0030395136778, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17539532, Length=334, Percent_Identity=24.251497005988, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI133901790, Length=330, Percent_Identity=25.1515151515152, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI133901786, Length=330, Percent_Identity=25.1515151515152, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17539424, Length=330, Percent_Identity=25.1515151515152, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI133901788, Length=330, Percent_Identity=25.1515151515152, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17539422, Length=330, Percent_Identity=25.1515151515152, Blast_Score=85, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17507723, Length=330, Percent_Identity=25.1515151515152, Blast_Score=84, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI71982035, Length=343, Percent_Identity=27.6967930029155, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI71982038, Length=345, Percent_Identity=27.536231884058, Blast_Score=81, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6319493, Length=347, Percent_Identity=24.4956772334294, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI21356223, Length=331, Percent_Identity=25.6797583081571, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24158427, Length=336, Percent_Identity=26.1904761904762, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI19923002, Length=362, Percent_Identity=24.8618784530387, Blast_Score=72, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 39673; Mature: 39673

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVIVTGGAGFIGSNLVRLLLERGHEVLNIDALTYAGNIHSLDGSADSPAYRFLHADVRD
CEEEEECCCCHHHHHHHHHHHHCCCCEEEEEEEEEECCEEECCCCCCCCHHHEEECCHHH
ASLMRRTLREYAPDWVMHLAAESHVDRSIDDPGNFMTTNVMGTFSMLQAALEYYRSLEGE
HHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCC
KKERFRFHHISTDEVYGSLGKEGLFTESTPYRPHSPYSASKASSDHLVRAWHDTYGLPVL
HHHHEEEECCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEE
ITNCSNNYGPYQFPEKLIPVVILKALGGEPIPVYGKGENVRDWLYVGDHCEALLTVVSCG
EECCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCHHEEECHHHHHHHHHHHCC
KPGETYNIGGNNEKTNLELVELLCSHLDELRPRPDGRSYREQISFVQDRPGHDLRYAIDA
CCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCEEEEECH
SKIRRELGWTPRQDHSSGFRKTIQWYLDHEEWWRGILSGEYRLERLGTNG
HHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHCCCCC
>Mature Secondary Structure
MKVIVTGGAGFIGSNLVRLLLERGHEVLNIDALTYAGNIHSLDGSADSPAYRFLHADVRD
CEEEEECCCCHHHHHHHHHHHHCCCCEEEEEEEEEECCEEECCCCCCCCHHHEEECCHHH
ASLMRRTLREYAPDWVMHLAAESHVDRSIDDPGNFMTTNVMGTFSMLQAALEYYRSLEGE
HHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCC
KKERFRFHHISTDEVYGSLGKEGLFTESTPYRPHSPYSASKASSDHLVRAWHDTYGLPVL
HHHHEEEECCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEE
ITNCSNNYGPYQFPEKLIPVVILKALGGEPIPVYGKGENVRDWLYVGDHCEALLTVVSCG
EECCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCHHEEECHHHHHHHHHHHCC
KPGETYNIGGNNEKTNLELVELLCSHLDELRPRPDGRSYREQISFVQDRPGHDLRYAIDA
CCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCEEEEECH
SKIRRELGWTPRQDHSSGFRKTIQWYLDHEEWWRGILSGEYRLERLGTNG
HHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8022265; 10710307 [H]