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The map label for this gene is gph [C]

Identifier: 187734692

GI number: 187734692

Start: 226864

End: 227565

Strand: Reverse

Name: gph [C]

Synonym: Amuc_0179

Alternate gene names: 187734692

Gene position: 227565-226864 (Counterclockwise)

Preceding gene: 187734693

Following gene: 187734689

Centisome position: 8.54

GC content: 61.82

Gene sequence:

>702_bases
ATGAAAACGGGTTTTGTCTTTGACCTGGACGGGACGCTGGTGGACTCTATTCCCGGTATCGCCCGCGGCCTGAATCTGGC
CTTGAAAGCGCTGGGGTATCCGGAGCATTCCGTGGACGCCATCCGGGAAATGGTGGGCAAGGGGGCCCGGGAGCTTTGCC
TGGCTTCCCTGAAAGGCTATTTCAACGGAAACGTGCCGGAGGAAGCTTTTGAAGCGGTGCACCGGGGATTCATGCGTGAA
TACCCGCACACCTGGCAGGAGGGCACGGTTCCGTACCCCGGCATCCGGGAAATGCTGTTGTCCCTGGCCGGAGAAGGCCA
TCCTCTGGGCGTTTTATCCAACAAGCCCCATGCGGTTACGGTGCCGCTGGTGCGCCATATCCTGCCTGAAATTCCCTTCC
GGGAGGTGATGGGTTTTTCCGAACGCTTTCCGCGCAAGCCGGAACCTGACTCCCTGCTGTCCATCGTCCGTTCCTGGGGC
AGGGAGCCGGCGCAGGTGTGCATGGTGGGGGACTCCGCCCATGACGGCAACACGGCCGTGAACGCCGGAACACGGCTTAT
TCTGGTGGGGTGGGGCTACAGTTCCCGTTCCGCTCTGGATGCCTTCCGGGTGCCGGTGTGCGCCGCCGCGGGGGAATTAT
CTGCCCGGCTGCATGATGAAAGCAGCCTGCCGTTTCCCGTTGCCGCGCATAGGAATTGTTGA

Upstream 100 bases:

>100_bases
CTGGAGGAACTGCGGCATTCGGGGTATGCGGAAAATGCCTGCCTGAGGAGAATCATCCAATTCGTGGAAACTTCCGAACG
GGGGTTCTGCCATTGAATCC

Downstream 100 bases:

>100_bases
AAATTCCGGGAAGTGCAGGGCCGCCGGAGGCTGAATTCCTCTACATCTCCAAGATGCTTTCAAGCGCAGGAAAGCGTTTG
CGGGGGATGCCTTAGAGCTG

Product: HAD-superfamily hydrolase, subfamily IA, variant 3

Products: NA

Alternate protein names: PGP; PGPase [H]

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MKTGFVFDLDGTLVDSIPGIARGLNLALKALGYPEHSVDAIREMVGKGARELCLASLKGYFNGNVPEEAFEAVHRGFMRE
YPHTWQEGTVPYPGIREMLLSLAGEGHPLGVLSNKPHAVTVPLVRHILPEIPFREVMGFSERFPRKPEPDSLLSIVRSWG
REPAQVCMVGDSAHDGNTAVNAGTRLILVGWGYSSRSALDAFRVPVCAAAGELSARLHDESSLPFPVAAHRNC

Sequences:

>Translated_233_residues
MKTGFVFDLDGTLVDSIPGIARGLNLALKALGYPEHSVDAIREMVGKGARELCLASLKGYFNGNVPEEAFEAVHRGFMRE
YPHTWQEGTVPYPGIREMLLSLAGEGHPLGVLSNKPHAVTVPLVRHILPEIPFREVMGFSERFPRKPEPDSLLSIVRSWG
REPAQVCMVGDSAHDGNTAVNAGTRLILVGWGYSSRSALDAFRVPVCAAAGELSARLHDESSLPFPVAAHRNC
>Mature_233_residues
MKTGFVFDLDGTLVDSIPGIARGLNLALKALGYPEHSVDAIREMVGKGARELCLASLKGYFNGNVPEEAFEAVHRGFMRE
YPHTWQEGTVPYPGIREMLLSLAGEGHPLGVLSNKPHAVTVPLVRHILPEIPFREVMGFSERFPRKPEPDSLLSIVRSWG
REPAQVCMVGDSAHDGNTAVNAGTRLILVGWGYSSRSALDAFRVPVCAAAGELSARLHDESSLPFPVAAHRNC

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=213, Percent_Identity=32.8638497652582, Blast_Score=103, Evalue=7e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833
- InterPro:   IPR006346
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.18 [H]

Molecular weight: Translated: 25337; Mature: 25337

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTGFVFDLDGTLVDSIPGIARGLNLALKALGYPEHSVDAIREMVGKGARELCLASLKGY
CCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHH
FNGNVPEEAFEAVHRGFMREYPHTWQEGTVPYPGIREMLLSLAGEGHPLGVLSNKPHAVT
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEECCCCCEEE
VPLVRHILPEIPFREVMGFSERFPRKPEPDSLLSIVRSWGREPAQVCMVGDSAHDGNTAV
HHHHHHHHCCCCHHHHHCHHHHCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCEE
NAGTRLILVGWGYSSRSALDAFRVPVCAAAGELSARLHDESSLPFPVAAHRNC
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKTGFVFDLDGTLVDSIPGIARGLNLALKALGYPEHSVDAIREMVGKGARELCLASLKGY
CCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHH
FNGNVPEEAFEAVHRGFMREYPHTWQEGTVPYPGIREMLLSLAGEGHPLGVLSNKPHAVT
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEECCCCCEEE
VPLVRHILPEIPFREVMGFSERFPRKPEPDSLLSIVRSWGREPAQVCMVGDSAHDGNTAV
HHHHHHHHCCCCHHHHHCHHHHCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCEE
NAGTRLILVGWGYSSRSALDAFRVPVCAAAGELSARLHDESSLPFPVAAHRNC
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]