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The map label for this gene is pilT [H]

Identifier: 187734679

GI number: 187734679

Start: 203383

End: 204477

Strand: Direct

Name: pilT [H]

Synonym: Amuc_0166

Alternate gene names: 187734679

Gene position: 203383-204477 (Clockwise)

Preceding gene: 187734678

Following gene: 187734680

Centisome position: 7.63

GC content: 57.35

Gene sequence:

>1095_bases
ATGGCTGTCATCGACCAATACTTCAAATACCTCGTAGAAGCGGGCGGTTCCGACCTTCACCTCAGCGAAGGCCAGCCTCC
TAAAGTCCGCGTTAACGGGACCATCTCCGCCATCTCCGACGAAAATCTGGAAGGCCAGGCGTTCAAAAACCTTCTCGCGG
AAATATGCGATCCCCAGGCCTTCCAGAAATATCTGGAAGAAGGGGACCTGGACTTTGCCTATGAAATGGATGAAACATCC
CGTTTCCGCTGCAACTACTTCAAGCAGCAGCACGGCCTGGGGGCTGTCTTCCGCCTCATTCCCACCAAAATAGCCACGCT
GGAAGAACTGGGCGTGCCTACGGTCGTCAAGGAATTCGCCCATATGAGATCCGGCCTTGTACTGGTCACAGGGCCCACCG
GCTCCGGTAAATCCACCACGCTGGCCGCTATCATCGACTACATCAACAGCAACCAGGCGCGGCACATCATCACCGTGGAG
GAACCTATCGAATTCGTCCATCCCAATAAAAAATCCATCGTCACCCAGAGGGAAGTGCCTCTCCAGACGCCTTCCTTCGC
AGACGGCCTCCGGGCTTCCCTTCGTGAAGACTCCGACATCGTGCTGGTGGGTGAAATGCGCGACCTGGAAACCATCTCCC
TGGCGCTCACGGCGGCGGAAACCGGCCTTCTGGTATTCGGCACCCTGCACACGAACAACGCCAGCAAAACCATTGACCGT
ATCATTGACGTATTCCCGTCTGACCAGCAATCCCAGGTGCGCACCATGCTTGCGGGTTCCCTCCGCGGCGTCGTGGCGCA
GCTCTTGATGAAGCGCAGCGACAAACCGGGGCGCGTAGCCGTCAATGAAATTCTCTTTGCCACGCCGGCCGTGGCGGCCA
TCATCCGTGAAGGCGCCACGCAGAAAATCCCTGACGTCATCGTCGGCGGCAAGGCCATGGGCATGCAATTCATGGATGAC
GCCATCTGGCAAAAACTCCAACAGGGCATCGTCTCCCCGGAAGAAGCCTACATGAAGTGCATTGAAAAGAAGAGATTCCG
CAACTTCCTTCCTCCGGAATCCGCCCGCCTGGCAGACTCCGGCGGCGGAAACTAA

Upstream 100 bases:

>100_bases
CTCATCACCACGGAAGACTGCATCTTCCGCGCGGAAGACAAAACCATCATGAAATCCTTCCTGGGAATCAAGTAACCTTT
AATTTTGAAACACTCCTGTT

Downstream 100 bases:

>100_bases
GCTCTTTGCCCTCCTTCTCCTGCCATGTCTCCCACTGGAAAATTCACGCTCAGGCTTGTGATTTATGGAGCCTTCCTGCT
CTATCTGGTGGGAGACTTGT

Product: twitching motility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 364; Mature: 363

Protein sequence:

>364_residues
MAVIDQYFKYLVEAGGSDLHLSEGQPPKVRVNGTISAISDENLEGQAFKNLLAEICDPQAFQKYLEEGDLDFAYEMDETS
RFRCNYFKQQHGLGAVFRLIPTKIATLEELGVPTVVKEFAHMRSGLVLVTGPTGSGKSTTLAAIIDYINSNQARHIITVE
EPIEFVHPNKKSIVTQREVPLQTPSFADGLRASLREDSDIVLVGEMRDLETISLALTAAETGLLVFGTLHTNNASKTIDR
IIDVFPSDQQSQVRTMLAGSLRGVVAQLLMKRSDKPGRVAVNEILFATPAVAAIIREGATQKIPDVIVGGKAMGMQFMDD
AIWQKLQQGIVSPEEAYMKCIEKKRFRNFLPPESARLADSGGGN

Sequences:

>Translated_364_residues
MAVIDQYFKYLVEAGGSDLHLSEGQPPKVRVNGTISAISDENLEGQAFKNLLAEICDPQAFQKYLEEGDLDFAYEMDETS
RFRCNYFKQQHGLGAVFRLIPTKIATLEELGVPTVVKEFAHMRSGLVLVTGPTGSGKSTTLAAIIDYINSNQARHIITVE
EPIEFVHPNKKSIVTQREVPLQTPSFADGLRASLREDSDIVLVGEMRDLETISLALTAAETGLLVFGTLHTNNASKTIDR
IIDVFPSDQQSQVRTMLAGSLRGVVAQLLMKRSDKPGRVAVNEILFATPAVAAIIREGATQKIPDVIVGGKAMGMQFMDD
AIWQKLQQGIVSPEEAYMKCIEKKRFRNFLPPESARLADSGGGN
>Mature_363_residues
AVIDQYFKYLVEAGGSDLHLSEGQPPKVRVNGTISAISDENLEGQAFKNLLAEICDPQAFQKYLEEGDLDFAYEMDETSR
FRCNYFKQQHGLGAVFRLIPTKIATLEELGVPTVVKEFAHMRSGLVLVTGPTGSGKSTTLAAIIDYINSNQARHIITVEE
PIEFVHPNKKSIVTQREVPLQTPSFADGLRASLREDSDIVLVGEMRDLETISLALTAAETGLLVFGTLHTNNASKTIDRI
IDVFPSDQQSQVRTMLAGSLRGVVAQLLMKRSDKPGRVAVNEILFATPAVAAIIREGATQKIPDVIVGGKAMGMQFMDDA
IWQKLQQGIVSPEEAYMKCIEKKRFRNFLPPESARLADSGGGN

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=299, Percent_Identity=46.1538461538462, Blast_Score=273, Evalue=9e-75,
Organism=Escherichia coli, GI1789723, Length=196, Percent_Identity=39.7959183673469, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1786296, Length=282, Percent_Identity=30.8510638297872, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 39926; Mature: 39795

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVIDQYFKYLVEAGGSDLHLSEGQPPKVRVNGTISAISDENLEGQAFKNLLAEICDPQA
CCHHHHHHHHHHHCCCCEEEECCCCCCEEEECCEEEECCCCCCCHHHHHHHHHHHCCHHH
FQKYLEEGDLDFAYEMDETSRFRCNYFKQQHGLGAVFRLIPTKIATLEELGVPTVVKEFA
HHHHHHCCCCCEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHH
HMRSGLVLVTGPTGSGKSTTLAAIIDYINSNQARHIITVEEPIEFVHPNKKSIVTQREVP
HHHCCEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCHHHHCCCHHHEEECCCCC
LQTPSFADGLRASLREDSDIVLVGEMRDLETISLALTAAETGLLVFGTLHTNNASKTIDR
CCCCCHHHHHHHHHCCCCCEEEEECCCCCHHHHEEEEECCCCEEEEEEEECCCHHHHHHH
IIDVFPSDQQSQVRTMLAGSLRGVVAQLLMKRSDKPGRVAVNEILFATPAVAAIIREGAT
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHCCCC
QKIPDVIVGGKAMGMQFMDDAIWQKLQQGIVSPEEAYMKCIEKKRFRNFLPPESARLADS
CCCCCEEECCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCC
GGGN
CCCC
>Mature Secondary Structure 
AVIDQYFKYLVEAGGSDLHLSEGQPPKVRVNGTISAISDENLEGQAFKNLLAEICDPQA
CHHHHHHHHHHHCCCCEEEECCCCCCEEEECCEEEECCCCCCCHHHHHHHHHHHCCHHH
FQKYLEEGDLDFAYEMDETSRFRCNYFKQQHGLGAVFRLIPTKIATLEELGVPTVVKEFA
HHHHHHCCCCCEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHH
HMRSGLVLVTGPTGSGKSTTLAAIIDYINSNQARHIITVEEPIEFVHPNKKSIVTQREVP
HHHCCEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCHHHHCCCHHHEEECCCCC
LQTPSFADGLRASLREDSDIVLVGEMRDLETISLALTAAETGLLVFGTLHTNNASKTIDR
CCCCCHHHHHHHHHCCCCCEEEEECCCCCHHHHEEEEECCCCEEEEEEEECCCHHHHHHH
IIDVFPSDQQSQVRTMLAGSLRGVVAQLLMKRSDKPGRVAVNEILFATPAVAAIIREGAT
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHCCCC
QKIPDVIVGGKAMGMQFMDDAIWQKLQQGIVSPEEAYMKCIEKKRFRNFLPPESARLADS
CCCCCEEECCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCC
GGGN
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]