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The map label for this gene is pilT [H]

Identifier: 187734678

GI number: 187734678

Start: 202275

End: 203357

Strand: Direct

Name: pilT [H]

Synonym: Amuc_0165

Alternate gene names: 187734678

Gene position: 202275-203357 (Clockwise)

Preceding gene: 187734677

Following gene: 187734679

Centisome position: 7.59

GC content: 59.0

Gene sequence:

>1083_bases
ATGAGCGAATACATTTTGCCCCACGTCGACGGATATCTCAAACTTGGACAGGATTATGACTGTTCCGATATCCACCTTGC
CGTCAACAGCCGTCCCACCTGGCGCCGGTTCGGCCAGCTTCTCCCCATCTGGGAGGAAGCGCCCAATCTCACGCCCCAGG
ACACGGAAGTGCTGGCGAGGGGCTTTCTGGAAGACCCGGAATGGAACCGCCTTCAGCAACGGGGGGACGTGGACTTCGCC
TACGCCAATGACTTTGGCCGCTACCGCGCATCCGTGGTTAAACAGCGCCTGGGTTATGACATCTGCTTCCGCATCATCAA
CACGCGGGTGCGCACCATGGAGGAAATAGGCCTGCCGACAGAATACGTGGTGCCCCTCACCCGCTACACCAACGGTCTCA
TTTTAGTCACGGGCTCCGTAGGTTCCGGTAAATCCACCACGCTGGCCTCTATCGTGGATTTCATCAACCAGGACCGCCAC
GACCACATCATCACGCTTGAAGACCCCATCGAATACCTGATTCCCTCCAAAAACTGCCACGTCAACCAGCGTGAAGTGCA
TAAACATACGGAATCCTTCGCGCGGGCCCTCCGCGGAGCCCTCCGTGAAGACCCAGACGTCATCATGGTGGGCGAAATGC
GCGACCTGGAAACCATCTCCCTGGCGCTCACGGCGGCAGAAACCGGCCACCTGGTGCTCGGCACCCTGCATACGGGTTCC
GCAGCCCGTACGGTGGACCGCGTGCTGGACGTCTTCCCGGTGGAACAACGCGACCAGATACGCATCATGGTCAGCGAATC
CCTGCGCGGCATCATCTCCCAGCAGCTGGTTCCCAGAGCAGACGGCAACGGCCGCGCTCTAGCCATTGAAATGCTGGTTA
ATACGCCGGCTATCGCCAACTGCATCCGCGAAGGCAAGACATTCATGATTCCCGGCTGCATCCAGACAGGGAAAGCCCAG
GGCATGATCCTGATGGACGACTCGCTCCGGGCCCTGCACCAGGCGGGCCTCATCACCACGGAAGACTGCATCTTCCGCGC
GGAAGACAAAACCATCATGAAATCCTTCCTGGGAATCAAGTAA

Upstream 100 bases:

>100_bases
AAGCCGCTTTCACGGAAATCGGTACCCAGACATTTTTTGCTGGTCAAAAAAGCTCTGTTTTCGTTAGTATGCAGCTTTAT
CAGTTTGCTGCATTCACAAC

Downstream 100 bases:

>100_bases
CCTTTAATTTTGAAACACTCCTGTTATGGCTGTCATCGACCAATACTTCAAATACCTCGTAGAAGCGGGCGGTTCCGACC
TTCACCTCAGCGAAGGCCAG

Product: twitching motility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 360; Mature: 359

Protein sequence:

>360_residues
MSEYILPHVDGYLKLGQDYDCSDIHLAVNSRPTWRRFGQLLPIWEEAPNLTPQDTEVLARGFLEDPEWNRLQQRGDVDFA
YANDFGRYRASVVKQRLGYDICFRIINTRVRTMEEIGLPTEYVVPLTRYTNGLILVTGSVGSGKSTTLASIVDFINQDRH
DHIITLEDPIEYLIPSKNCHVNQREVHKHTESFARALRGALREDPDVIMVGEMRDLETISLALTAAETGHLVLGTLHTGS
AARTVDRVLDVFPVEQRDQIRIMVSESLRGIISQQLVPRADGNGRALAIEMLVNTPAIANCIREGKTFMIPGCIQTGKAQ
GMILMDDSLRALHQAGLITTEDCIFRAEDKTIMKSFLGIK

Sequences:

>Translated_360_residues
MSEYILPHVDGYLKLGQDYDCSDIHLAVNSRPTWRRFGQLLPIWEEAPNLTPQDTEVLARGFLEDPEWNRLQQRGDVDFA
YANDFGRYRASVVKQRLGYDICFRIINTRVRTMEEIGLPTEYVVPLTRYTNGLILVTGSVGSGKSTTLASIVDFINQDRH
DHIITLEDPIEYLIPSKNCHVNQREVHKHTESFARALRGALREDPDVIMVGEMRDLETISLALTAAETGHLVLGTLHTGS
AARTVDRVLDVFPVEQRDQIRIMVSESLRGIISQQLVPRADGNGRALAIEMLVNTPAIANCIREGKTFMIPGCIQTGKAQ
GMILMDDSLRALHQAGLITTEDCIFRAEDKTIMKSFLGIK
>Mature_359_residues
SEYILPHVDGYLKLGQDYDCSDIHLAVNSRPTWRRFGQLLPIWEEAPNLTPQDTEVLARGFLEDPEWNRLQQRGDVDFAY
ANDFGRYRASVVKQRLGYDICFRIINTRVRTMEEIGLPTEYVVPLTRYTNGLILVTGSVGSGKSTTLASIVDFINQDRHD
HIITLEDPIEYLIPSKNCHVNQREVHKHTESFARALRGALREDPDVIMVGEMRDLETISLALTAAETGHLVLGTLHTGSA
ARTVDRVLDVFPVEQRDQIRIMVSESLRGIISQQLVPRADGNGRALAIEMLVNTPAIANCIREGKTFMIPGCIQTGKAQG
MILMDDSLRALHQAGLITTEDCIFRAEDKTIMKSFLGIK

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=327, Percent_Identity=46.7889908256881, Blast_Score=270, Evalue=2e-73,
Organism=Escherichia coli, GI1789723, Length=271, Percent_Identity=36.1623616236162, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1786296, Length=215, Percent_Identity=35.8139534883721, Blast_Score=109, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 40418; Mature: 40287

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEYILPHVDGYLKLGQDYDCSDIHLAVNSRPTWRRFGQLLPIWEEAPNLTPQDTEVLAR
CCCCCCCCCCCCEECCCCCCCCCEEEEECCCCCHHHHCCCCCCHHHCCCCCCCHHHHHHH
GFLEDPEWNRLQQRGDVDFAYANDFGRYRASVVKQRLGYDICFRIINTRVRTMEEIGLPT
HCCCCCCHHHHHHCCCCCEEECCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCH
EYVVPLTRYTNGLILVTGSVGSGKSTTLASIVDFINQDRHDHIITLEDPIEYLIPSKNCH
HHEEEHHHCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCEEEECCCHHHHCCCCCCC
VNQREVHKHTESFARALRGALREDPDVIMVGEMRDLETISLALTAAETGHLVLGTLHTGS
CCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCEEEEEEEECCCCCEEEEEECCCC
AARTVDRVLDVFPVEQRDQIRIMVSESLRGIISQQLVPRADGNGRALAIEMLVNTPAIAN
HHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCHHHHH
CIREGKTFMIPGCIQTGKAQGMILMDDSLRALHQAGLITTEDCIFRAEDKTIMKSFLGIK
HHHCCCEEEECCCCCCCCCCCEEEECHHHHHHHHCCCEEEHHHEEECCCHHHHHHHHCCC
>Mature Secondary Structure 
SEYILPHVDGYLKLGQDYDCSDIHLAVNSRPTWRRFGQLLPIWEEAPNLTPQDTEVLAR
CCCCCCCCCCCEECCCCCCCCCEEEEECCCCCHHHHCCCCCCHHHCCCCCCCHHHHHHH
GFLEDPEWNRLQQRGDVDFAYANDFGRYRASVVKQRLGYDICFRIINTRVRTMEEIGLPT
HCCCCCCHHHHHHCCCCCEEECCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCH
EYVVPLTRYTNGLILVTGSVGSGKSTTLASIVDFINQDRHDHIITLEDPIEYLIPSKNCH
HHEEEHHHCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCEEEECCCHHHHCCCCCCC
VNQREVHKHTESFARALRGALREDPDVIMVGEMRDLETISLALTAAETGHLVLGTLHTGS
CCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCEEEEEEEECCCCCEEEEEECCCC
AARTVDRVLDVFPVEQRDQIRIMVSESLRGIISQQLVPRADGNGRALAIEMLVNTPAIAN
HHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCHHHHH
CIREGKTFMIPGCIQTGKAQGMILMDDSLRALHQAGLITTEDCIFRAEDKTIMKSFLGIK
HHHCCCEEEECCCCCCCCCCCEEEECHHHHHHHHCCCEEEHHHEEECCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]