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The map label for this gene is oppA [H]

Identifier: 187734658

GI number: 187734658

Start: 176262

End: 178121

Strand: Reverse

Name: oppA [H]

Synonym: Amuc_0145

Alternate gene names: 187734658

Gene position: 178121-176262 (Counterclockwise)

Preceding gene: 187734660

Following gene: 187734657

Centisome position: 6.69

GC content: 60.97

Gene sequence:

>1860_bases
ATGCGCCTGTTCATGATGTTCTGTGTGATGCTTTTGCTCGCCGGATGCGATACGCGCACGAGCGTGGAACGGGCGCAGGA
GGCAGGGGTGCTCATCGTGGGCAATAATGCGGAGCCCCAGAGCTTTGACCCCCACATCGCCACTTCCGTTTCCGATTTCA
AGATGATCAATGCCGTCATGGAGGGGCTGCTGCGCGGGGATTCCAGGGATGACGCCGTTTTTCATCCCGCTGTGGCGGAG
TCGTGGACGCATAGTCCGGAGGCGGACAAGTGGCGTTTTTCCCTCCGGAAGGACGCCGTGTGGAGCGACGGCGTTCCTGT
GACGGCCCATGATTTCGTGTATGCCTATCACCGCCTGCTTCATCCGGGGTTTGGAGGGAGGTACGCGGACATGCTTTATC
CGCTGAAGAATGCGGAAGCCTATAACCGGAACAGGCGCAGCCTGCTGCTGTGCGGTCCCGGTTCCCGGCTGGCTGGGGAA
GAGGGGCTGGAAGAGAGGGTTCTGGCCCGGGTGGATTGGGAGAGGCTGGATGGTATGGGAGCCGCTGAGCTGGAGGCTCT
GCGGGATGATCCTACCCTGATGGAGTGGCCGGACGGGATGCCGGAGGAAGGAGCGCGGAAGATTGCGGCCCGGATGTTGG
AGGATGTCCGGGCCGGAAAGCCGGATTTATGGGAGGAAGCCCGCGTGGGCGTGCGCGCCGCGGATGATTATACCCTGGAG
ATGGAGCTGCGTTCTCCGATGCCCCAGCTTCCCCTGCTGTTGCTTCACTGCACCTGGTTTCCGGTTCCCCGCCATGCCGT
GGAAGCCCACGGCGGCATGCTGGACAGGACGGGGAAATGGACGCGGCCCGGAGCGGCCGTAGGGAACGGACCTTTTCTTA
TGGCGGAGCACCGTTTCAATGATTATGTGGAGGTGCGCCGCAATCCCCGGTACCGGAACGCTTCCGCCGTCCGGCTGAAT
GGAGTCCGTTTTCTTCCCACCGTGAACGGCTTTACGGAAACCCGCATGTTTTTCAACGGCAAGCTTCATGTCACCAACAA
CGTGCCTCCGGAGATGACGGCTTACGCCCGGGAGCGCGGCGGCGATGATTTTTGCCAGGATGATTATTACGTGACTATTT
TTTACCGTCTGAATACGTCGCGTGCGCCGCTGAATGACGCCCGGGTCAGGCGGGCCCTTTCCCTGGCGGTAGACCGGGAG
GCTCTGGTGCGGGACGTGGTGTGCGGCGGCGGCCAGCCGTGCTTCGGCTTTACGCCGGACGGCGCCGGATACAAGACGCC
GCATGGGGTGGAGTTCAATCCGGAGAAAGCGCGTTCCCTGCTGGCCCAGGCCGGTTTTCCCGGCGGGAAGGGTTTTCCCC
GGCTGGAGCTGATGACTACATCCCGCGAAGTTCAGAAGACGATGGCGGAAGCCATCCAGGGCATGTGGAAAAAGCATCTG
GGGATTCATGTGGATATCCGTTCCTGTGAGTGGACGGCCTACAAGTTCGCGCAGAATTCCATGCAGTATGATTTTAGTTC
CTCCTCATGGTCCGGTGATTATCTGGACCCTTCCAGTTTTCTGGATTTGTGGGGTTCCGCCTCCGGGAACAATAATACGG
GCTGGTTTCATCCGGAGTATGAACGCCTGCTGGCGGAGAGCCGCGCCACGGGAGACCAGGAGAAGCGCATGGCCCTGCTG
GCCCGTGCGGAAGCGCTGATGCTTTCCGAATCCCCGGTGATTCCCCTGTATTGGGCCAAACGGTCCTATTTGAAGAGGCC
GGAGGTGCGGGGATGGCACCCCCTGCTGCTGGATAACCATTTGTTTGAGGATATCAGCCTGGACGACGCCCGGGAACATG
GGAAGGAGGCCGCGCCTTGA

Upstream 100 bases:

>100_bases
CTTCCGCTTCTGGGCGGGCGCTCAGGTCGTTGTGGTTCTGCCGCCGTATTTCTTGACGTGAAGCCTTGCGTACGGGAAGG
AAACCTGACAGAATCCCGCC

Downstream 100 bases:

>100_bases
CCAGGATGATTCTGAAACGCCTGGGGCAGGGGCTTCTGGTCCTGCTGGTGCTGGAAACTGTGACGTTTTTCCTGATACGC
CTGTTGCCGGGGCACCCCTT

Product: 4-phytase

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 619; Mature: 619

Protein sequence:

>619_residues
MRLFMMFCVMLLLAGCDTRTSVERAQEAGVLIVGNNAEPQSFDPHIATSVSDFKMINAVMEGLLRGDSRDDAVFHPAVAE
SWTHSPEADKWRFSLRKDAVWSDGVPVTAHDFVYAYHRLLHPGFGGRYADMLYPLKNAEAYNRNRRSLLLCGPGSRLAGE
EGLEERVLARVDWERLDGMGAAELEALRDDPTLMEWPDGMPEEGARKIAARMLEDVRAGKPDLWEEARVGVRAADDYTLE
MELRSPMPQLPLLLLHCTWFPVPRHAVEAHGGMLDRTGKWTRPGAAVGNGPFLMAEHRFNDYVEVRRNPRYRNASAVRLN
GVRFLPTVNGFTETRMFFNGKLHVTNNVPPEMTAYARERGGDDFCQDDYYVTIFYRLNTSRAPLNDARVRRALSLAVDRE
ALVRDVVCGGGQPCFGFTPDGAGYKTPHGVEFNPEKARSLLAQAGFPGGKGFPRLELMTTSREVQKTMAEAIQGMWKKHL
GIHVDIRSCEWTAYKFAQNSMQYDFSSSSWSGDYLDPSSFLDLWGSASGNNNTGWFHPEYERLLAESRATGDQEKRMALL
ARAEALMLSESPVIPLYWAKRSYLKRPEVRGWHPLLLDNHLFEDISLDDAREHGKEAAP

Sequences:

>Translated_619_residues
MRLFMMFCVMLLLAGCDTRTSVERAQEAGVLIVGNNAEPQSFDPHIATSVSDFKMINAVMEGLLRGDSRDDAVFHPAVAE
SWTHSPEADKWRFSLRKDAVWSDGVPVTAHDFVYAYHRLLHPGFGGRYADMLYPLKNAEAYNRNRRSLLLCGPGSRLAGE
EGLEERVLARVDWERLDGMGAAELEALRDDPTLMEWPDGMPEEGARKIAARMLEDVRAGKPDLWEEARVGVRAADDYTLE
MELRSPMPQLPLLLLHCTWFPVPRHAVEAHGGMLDRTGKWTRPGAAVGNGPFLMAEHRFNDYVEVRRNPRYRNASAVRLN
GVRFLPTVNGFTETRMFFNGKLHVTNNVPPEMTAYARERGGDDFCQDDYYVTIFYRLNTSRAPLNDARVRRALSLAVDRE
ALVRDVVCGGGQPCFGFTPDGAGYKTPHGVEFNPEKARSLLAQAGFPGGKGFPRLELMTTSREVQKTMAEAIQGMWKKHL
GIHVDIRSCEWTAYKFAQNSMQYDFSSSSWSGDYLDPSSFLDLWGSASGNNNTGWFHPEYERLLAESRATGDQEKRMALL
ARAEALMLSESPVIPLYWAKRSYLKRPEVRGWHPLLLDNHLFEDISLDDAREHGKEAAP
>Mature_619_residues
MRLFMMFCVMLLLAGCDTRTSVERAQEAGVLIVGNNAEPQSFDPHIATSVSDFKMINAVMEGLLRGDSRDDAVFHPAVAE
SWTHSPEADKWRFSLRKDAVWSDGVPVTAHDFVYAYHRLLHPGFGGRYADMLYPLKNAEAYNRNRRSLLLCGPGSRLAGE
EGLEERVLARVDWERLDGMGAAELEALRDDPTLMEWPDGMPEEGARKIAARMLEDVRAGKPDLWEEARVGVRAADDYTLE
MELRSPMPQLPLLLLHCTWFPVPRHAVEAHGGMLDRTGKWTRPGAAVGNGPFLMAEHRFNDYVEVRRNPRYRNASAVRLN
GVRFLPTVNGFTETRMFFNGKLHVTNNVPPEMTAYARERGGDDFCQDDYYVTIFYRLNTSRAPLNDARVRRALSLAVDRE
ALVRDVVCGGGQPCFGFTPDGAGYKTPHGVEFNPEKARSLLAQAGFPGGKGFPRLELMTTSREVQKTMAEAIQGMWKKHL
GIHVDIRSCEWTAYKFAQNSMQYDFSSSSWSGDYLDPSSFLDLWGSASGNNNTGWFHPEYERLLAESRATGDQEKRMALL
ARAEALMLSESPVIPLYWAKRSYLKRPEVRGWHPLLLDNHLFEDISLDDAREHGKEAAP

Specific function: This protein is a component of the oligopeptide permease, a binding protein-dependent transport system, it binds peptides up to five amino acids long with high affinity [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=372, Percent_Identity=31.989247311828, Blast_Score=223, Evalue=3e-59,
Organism=Escherichia coli, GI87081878, Length=373, Percent_Identity=30.0268096514745, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI1789397, Length=384, Percent_Identity=31.7708333333333, Blast_Score=184, Evalue=2e-47,
Organism=Escherichia coli, GI1789966, Length=245, Percent_Identity=30.2040816326531, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1787052, Length=402, Percent_Identity=21.1442786069652, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787762, Length=383, Percent_Identity=21.6710182767624, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI1789887, Length=332, Percent_Identity=24.6987951807229, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 69768; Mature: 69768

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLFMMFCVMLLLAGCDTRTSVERAQEAGVLIVGNNAEPQSFDPHIATSVSDFKMINAVM
CHHHHHHHHHHHHCCCCCHHHHHHHHHCCEEEECCCCCCCCCCCHHHCCHHHHHHHHHHH
EGLLRGDSRDDAVFHPAVAESWTHSPEADKWRFSLRKDAVWSDGVPVTAHDFVYAYHRLL
HHHHCCCCCCCCEECCHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
HPGFGGRYADMLYPLKNAEAYNRNRRSLLLCGPGSRLAGEEGLEERVLARVDWERLDGMG
CCCCCCCHHHHHHCCCCCHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHCHHHHCCCC
AAELEALRDDPTLMEWPDGMPEEGARKIAARMLEDVRAGKPDLWEEARVGVRAADDYTLE
HHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHCCCEEECCCEEEE
MELRSPMPQLPLLLLHCTWFPVPRHAVEAHGGMLDRTGKWTRPGAAVGNGPFLMAEHRFN
EECCCCCCHHHHHHHHHCCCCCCHHHHHHCCCEECCCCCCCCCCCCCCCCCEEEEECCCC
DYVEVRRNPRYRNASAVRLNGVRFLPTVNGFTETRMFFNGKLHVTNNVPPEMTAYARERG
CHHHHHCCCCCCCCCEEEECCEEEECCCCCCCCEEEEECCEEEECCCCCCHHHHHHHHCC
GDDFCQDDYYVTIFYRLNTSRAPLNDARVRRALSLAVDREALVRDVVCGGGQPCFGFTPD
CCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
GAGYKTPHGVEFNPEKARSLLAQAGFPGGKGFPRLELMTTSREVQKTMAEAIQGMWKKHL
CCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHC
GIHVDIRSCEWTAYKFAQNSMQYDFSSSSWSGDYLDPSSFLDLWGSASGNNNTGWFHPEY
CEEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHH
ERLLAESRATGDQEKRMALLARAEALMLSESPVIPLYWAKRSYLKRPEVRGWHPLLLDNH
HHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHCCCCCCCCCCCCCCCH
LFEDISLDDAREHGKEAAP
HHHCCCCCHHHHCCCCCCC
>Mature Secondary Structure
MRLFMMFCVMLLLAGCDTRTSVERAQEAGVLIVGNNAEPQSFDPHIATSVSDFKMINAVM
CHHHHHHHHHHHHCCCCCHHHHHHHHHCCEEEECCCCCCCCCCCHHHCCHHHHHHHHHHH
EGLLRGDSRDDAVFHPAVAESWTHSPEADKWRFSLRKDAVWSDGVPVTAHDFVYAYHRLL
HHHHCCCCCCCCEECCHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
HPGFGGRYADMLYPLKNAEAYNRNRRSLLLCGPGSRLAGEEGLEERVLARVDWERLDGMG
CCCCCCCHHHHHHCCCCCHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHCHHHHCCCC
AAELEALRDDPTLMEWPDGMPEEGARKIAARMLEDVRAGKPDLWEEARVGVRAADDYTLE
HHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHCCCEEECCCEEEE
MELRSPMPQLPLLLLHCTWFPVPRHAVEAHGGMLDRTGKWTRPGAAVGNGPFLMAEHRFN
EECCCCCCHHHHHHHHHCCCCCCHHHHHHCCCEECCCCCCCCCCCCCCCCCEEEEECCCC
DYVEVRRNPRYRNASAVRLNGVRFLPTVNGFTETRMFFNGKLHVTNNVPPEMTAYARERG
CHHHHHCCCCCCCCCEEEECCEEEECCCCCCCCEEEEECCEEEECCCCCCHHHHHHHHCC
GDDFCQDDYYVTIFYRLNTSRAPLNDARVRRALSLAVDREALVRDVVCGGGQPCFGFTPD
CCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
GAGYKTPHGVEFNPEKARSLLAQAGFPGGKGFPRLELMTTSREVQKTMAEAIQGMWKKHL
CCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHC
GIHVDIRSCEWTAYKFAQNSMQYDFSSSSWSGDYLDPSSFLDLWGSASGNNNTGWFHPEY
CEEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHH
ERLLAESRATGDQEKRMALLARAEALMLSESPVIPLYWAKRSYLKRPEVRGWHPLLLDNH
HHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHCCCCCCCCCCCCCCCH
LFEDISLDDAREHGKEAAP
HHHCCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2821267; 3525163; 11677609; 8202710; 8747465; 10422831; 10438628 [H]