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The map label for this gene is pyrB

Identifier: 187734626

GI number: 187734626

Start: 140311

End: 141240

Strand: Reverse

Name: pyrB

Synonym: Amuc_0112

Alternate gene names: 187734626

Gene position: 141240-140311 (Counterclockwise)

Preceding gene: 187734627

Following gene: 187734625

Centisome position: 5.3

GC content: 57.85

Gene sequence:

>930_bases
ATGAATCATCCCCGCAAGGATCTTCTTAATATCTCCTCATTGACTGACGAGGAAATTCACACGCTGCTGGATTCGGCCGG
CCCGATGAAGGAGCTTTTTACCAAAAGCGTCAAAAAGGTGCCCGCCCTGAAAGGCAAGTCCGTTCTGACGCTTTTTTATG
AACCCAGCACCCGCACCCGTTCCTCTTTTGAGGTAGCGGCGGAACGCCTGTCTGCGGACGTGACCAATTTCACGGTGTCC
ACCTCTTCCGTGGTGAAAGGGGAATCCGTACAGGACACGATCGCCACGCTCCAGGCCATGAAAGTGGATTATGTAATCGT
GCGCCATTACAACAGCGGCTTGCCGAACGTGATTGCGCGCCATACCTGCGCCAGTGTGGTGAACGCCGGGGACGGAGCCC
ACGCCCATCCCTCTCAGGCGCTGCTGGATTCCTTTACCATCCGGGAGGTGTTTCCGGATGTGAGGGGAAAGCGCGTTCTG
ATTGTGGGGGACATCCTGCATTCCCGCGTGGCCCGTTCCACCTCCCTGCTGATGAAGAGGCTGGGGATGGAGGTCGCCTT
CCTGGGGCCGGGTTCCCTGGTGCCGCGTACCGAACATTCCGGCATTCCCCGTTTTTCCGATTTCAATGAGGCGTTTGCCT
GGAAGCCGGACGTAATTTACCTGCTGCGCGTTCAAAAAGAGCGGCAGGACGCCCCTTTCTTCCCCAGCGCCCGGGAGTAC
AACAAGATTTACGGCGTCACGGAAGAACGCCTGAAGCGCATTTCCGGTGAAGGCCTGTACATCATGCATCCGGGGCCGGT
CAACCGCGGCGTGGAAATCTGTGACCTGGCGATGGACTATGAGCGCTGCCTGATCAACCGCCAGGTGGAAAACGGCATCG
CCTGCCGCATGTCCATCCTTTACCATCTCACTCCGCAAACCCAGCACTGA

Upstream 100 bases:

>100_bases
GCCGGAGCCAAGGTCATCGTAAGCATGAAGGAGGCTGACGGCGAGGACAGCGTGGTCATTCCCTCCCACTGAAAATTTCC
CAATCCATTCTCCGTTTCCC

Downstream 100 bases:

>100_bases
TTGTTCATGAAAACTTCCGTACTTATTCGCAACGCCCGGCTGACGGACGGTTCCGCCCCGGCGGACCTTCTGGTTTCCGG
GGGGGTGATTACAGCGAAAG

Product: aspartate carbamoyltransferase

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MNHPRKDLLNISSLTDEEIHTLLDSAGPMKELFTKSVKKVPALKGKSVLTLFYEPSTRTRSSFEVAAERLSADVTNFTVS
TSSVVKGESVQDTIATLQAMKVDYVIVRHYNSGLPNVIARHTCASVVNAGDGAHAHPSQALLDSFTIREVFPDVRGKRVL
IVGDILHSRVARSTSLLMKRLGMEVAFLGPGSLVPRTEHSGIPRFSDFNEAFAWKPDVIYLLRVQKERQDAPFFPSAREY
NKIYGVTEERLKRISGEGLYIMHPGPVNRGVEICDLAMDYERCLINRQVENGIACRMSILYHLTPQTQH

Sequences:

>Translated_309_residues
MNHPRKDLLNISSLTDEEIHTLLDSAGPMKELFTKSVKKVPALKGKSVLTLFYEPSTRTRSSFEVAAERLSADVTNFTVS
TSSVVKGESVQDTIATLQAMKVDYVIVRHYNSGLPNVIARHTCASVVNAGDGAHAHPSQALLDSFTIREVFPDVRGKRVL
IVGDILHSRVARSTSLLMKRLGMEVAFLGPGSLVPRTEHSGIPRFSDFNEAFAWKPDVIYLLRVQKERQDAPFFPSAREY
NKIYGVTEERLKRISGEGLYIMHPGPVNRGVEICDLAMDYERCLINRQVENGIACRMSILYHLTPQTQH
>Mature_309_residues
MNHPRKDLLNISSLTDEEIHTLLDSAGPMKELFTKSVKKVPALKGKSVLTLFYEPSTRTRSSFEVAAERLSADVTNFTVS
TSSVVKGESVQDTIATLQAMKVDYVIVRHYNSGLPNVIARHTCASVVNAGDGAHAHPSQALLDSFTIREVFPDVRGKRVL
IVGDILHSRVARSTSLLMKRLGMEVAFLGPGSLVPRTEHSGIPRFSDFNEAFAWKPDVIYLLRVQKERQDAPFFPSAREY
NKIYGVTEERLKRISGEGLYIMHPGPVNRGVEICDLAMDYERCLINRQVENGIACRMSILYHLTPQTQH

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=306, Percent_Identity=34.9673202614379, Blast_Score=157, Evalue=9e-39,
Organism=Homo sapiens, GI38788445, Length=166, Percent_Identity=30.7228915662651, Blast_Score=69, Evalue=5e-12,
Organism=Escherichia coli, GI2367364, Length=311, Percent_Identity=30.5466237942122, Blast_Score=114, Evalue=6e-27,
Organism=Escherichia coli, GI1790703, Length=147, Percent_Identity=35.3741496598639, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1786469, Length=196, Percent_Identity=30.6122448979592, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI193204318, Length=323, Percent_Identity=31.2693498452012, Blast_Score=115, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6322331, Length=304, Percent_Identity=31.9078947368421, Blast_Score=132, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24642586, Length=306, Percent_Identity=32.0261437908497, Blast_Score=127, Evalue=1e-29,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_AKKM8 (B2ULR3)

Other databases:

- EMBL:   CP001071
- RefSeq:   YP_001876738.1
- ProteinModelPortal:   B2ULR3
- SMR:   B2ULR3
- GeneID:   6274932
- GenomeReviews:   CP001071_GR
- KEGG:   amu:Amuc_0112
- HOGENOM:   HBG579429
- OMA:   MNRGLEI
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 34523; Mature: 34523

Theoretical pI: Translated: 8.85; Mature: 8.85

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHPRKDLLNISSLTDEEIHTLLDSAGPMKELFTKSVKKVPALKGKSVLTLFYEPSTRTR
CCCCHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCH
SSFEVAAERLSADVTNFTVSTSSVVKGESVQDTIATLQAMKVDYVIVRHYNSGLPNVIAR
HHHHHHHHHHHHHHHEEEEECHHHHCCCCHHHHHHHHHHHHHCEEEEEECCCCCHHHHHH
HTCASVVNAGDGAHAHPSQALLDSFTIREVFPDVRGKRVLIVGDILHSRVARSTSLLMKR
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHH
LGMEVAFLGPGSLVPRTEHSGIPRFSDFNEAFAWKPDVIYLLRVQKERQDAPFFPSAREY
HCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHCCCCCEEEEEEEHHHCCCCCCCCCHHHH
NKIYGVTEERLKRISGEGLYIMHPGPVNRGVEICDLAMDYERCLINRQVENGIACRMSIL
HHHCCCCHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHEEEEH
YHLTPQTQH
EEECCCCCC
>Mature Secondary Structure
MNHPRKDLLNISSLTDEEIHTLLDSAGPMKELFTKSVKKVPALKGKSVLTLFYEPSTRTR
CCCCHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCH
SSFEVAAERLSADVTNFTVSTSSVVKGESVQDTIATLQAMKVDYVIVRHYNSGLPNVIAR
HHHHHHHHHHHHHHHEEEEECHHHHCCCCHHHHHHHHHHHHHCEEEEEECCCCCHHHHHH
HTCASVVNAGDGAHAHPSQALLDSFTIREVFPDVRGKRVLIVGDILHSRVARSTSLLMKR
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHH
LGMEVAFLGPGSLVPRTEHSGIPRFSDFNEAFAWKPDVIYLLRVQKERQDAPFFPSAREY
HCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHCCCCCEEEEEEEHHHCCCCCCCCCHHHH
NKIYGVTEERLKRISGEGLYIMHPGPVNRGVEICDLAMDYERCLINRQVENGIACRMSIL
HHHCCCCHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHEEEEH
YHLTPQTQH
EEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA