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The map label for this gene is rsmF [H]

Identifier: 187734612

GI number: 187734612

Start: 124157

End: 125125

Strand: Direct

Name: rsmF [H]

Synonym: Amuc_0098

Alternate gene names: 187734612

Gene position: 124157-125125 (Clockwise)

Preceding gene: 187734611

Following gene: 187734613

Centisome position: 4.66

GC content: 62.33

Gene sequence:

>969_bases
GTGTCTTCCTCCCAAATACTCAAACTCGCCCGCAAACTGGAATTGACGGCGGAACAGGAACGGGATTTCCTGGACGCCAT
GCAGGCCGGGAACCGTTCCAGAAGCGCGCTGGTCATCACCCCGAAGGCACCGGAAGGCTACAGGCCGCCCTTGCCGGCAA
AGGACACGCCGCAGGAGTGGGGGCATTCCTCCATCCTGGCGCTCCCGTCCGGAGAGGCGGAAACCAAACCCGGCTCCCTG
CCGGATTACGAATGCGGCTATTACTATCCCCTGGATCTTTCGTCCGTCTGGGAAACGGCCCCTCTGGCGCATCTTCCGTT
TCGTCCGGAACGCTGCCTGGACATGTGCGCGGCGCCGGGAGGGAAAAGCATTCTGGCGCAGACGCGGGTATCTCCGCAGG
AGCATGTTTCCAACGAAGTGAATCCGAAGCGCCTGGGCATTCTGCGCCACAACCTGCTTCGCTGCGGCTTCACCAATCTG
TACACCCAGCGCCTGCGCCCGGACCAGTGGGCGGAACTGGCCCCCGGGTGTTTTGACCTCATTCTGGCGGATGCCCCGTG
CAGCGGGCAGTCCCTGCTGGCCAAAGGCATTCCCAACCCCGGCTGTTTCAATTCCTCCGTCACCGGAGGCAACGCCAAGC
GGCAGCGGGGCATTTTGCTGGCCGCCCTCCGGTGTCTGGCTCCCGGCGGCTTCCTGCTGTACACTACATGCACTTACGCT
CCGGAGGAAAATGAACGCAATGTCCTGTACCTGCTGAAACGCCATCCGGACCTGCACACCATAACCGTGCCGGAGCTGGA
GCATTTCCGTTCCTCCCTGACGCAGGAAGCCTGCTACCGGCTCATGCCCTTCCACGGAGCAGGAGCGGGCGGTTTCACCT
GCCTGCTGCGCAAGGAGGGAGGCCATCCGCTCCCGCTTTCCCTGCCGGATGAATTGAAGGCATGGCCCATTCATGCCATG
AATTGTTGA

Upstream 100 bases:

>100_bases
GAAATCCTTCCCGCCCAGTTCGGTACGGAGGCGGGCATCCTGGGGGCCGCCCATCTGGCTCTCAACACCCACTTCGGAGA
AACATTCCGGGCCTGATCTC

Downstream 100 bases:

>100_bases
AAAAAGATGAAGATAGGCATTTTTTCCGACTTGCACAACCGCACAGACCATCTGGAGCCAGCCATGCACCGGATGCGCCT
GCTGGGCTGCGGACATTTCA

Product: Fmu (Sun) domain protein

Products: NA

Alternate protein names: 16S rRNA m5C1407 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmF [H]

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MSSSQILKLARKLELTAEQERDFLDAMQAGNRSRSALVITPKAPEGYRPPLPAKDTPQEWGHSSILALPSGEAETKPGSL
PDYECGYYYPLDLSSVWETAPLAHLPFRPERCLDMCAAPGGKSILAQTRVSPQEHVSNEVNPKRLGILRHNLLRCGFTNL
YTQRLRPDQWAELAPGCFDLILADAPCSGQSLLAKGIPNPGCFNSSVTGGNAKRQRGILLAALRCLAPGGFLLYTTCTYA
PEENERNVLYLLKRHPDLHTITVPELEHFRSSLTQEACYRLMPFHGAGAGGFTCLLRKEGGHPLPLSLPDELKAWPIHAM
NC

Sequences:

>Translated_322_residues
MSSSQILKLARKLELTAEQERDFLDAMQAGNRSRSALVITPKAPEGYRPPLPAKDTPQEWGHSSILALPSGEAETKPGSL
PDYECGYYYPLDLSSVWETAPLAHLPFRPERCLDMCAAPGGKSILAQTRVSPQEHVSNEVNPKRLGILRHNLLRCGFTNL
YTQRLRPDQWAELAPGCFDLILADAPCSGQSLLAKGIPNPGCFNSSVTGGNAKRQRGILLAALRCLAPGGFLLYTTCTYA
PEENERNVLYLLKRHPDLHTITVPELEHFRSSLTQEACYRLMPFHGAGAGGFTCLLRKEGGHPLPLSLPDELKAWPIHAM
NC
>Mature_321_residues
SSSQILKLARKLELTAEQERDFLDAMQAGNRSRSALVITPKAPEGYRPPLPAKDTPQEWGHSSILALPSGEAETKPGSLP
DYECGYYYPLDLSSVWETAPLAHLPFRPERCLDMCAAPGGKSILAQTRVSPQEHVSNEVNPKRLGILRHNLLRCGFTNLY
TQRLRPDQWAELAPGCFDLILADAPCSGQSLLAKGIPNPGCFNSSVTGGNAKRQRGILLAALRCLAPGGFLLYTTCTYAP
EENERNVLYLLKRHPDLHTITVPELEHFRSSLTQEACYRLMPFHGAGAGGFTCLLRKEGGHPLPLSLPDELKAWPIHAMN
C

Specific function: Specifically methylates the cytosine at position 1407 (m5C1407) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=183, Percent_Identity=35.5191256830601, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI76150623, Length=183, Percent_Identity=35.5191256830601, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI11545785, Length=182, Percent_Identity=30.7692307692308, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI32698918, Length=168, Percent_Identity=30.952380952381, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI87081985, Length=255, Percent_Identity=30.1960784313725, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI2367212, Length=181, Percent_Identity=32.0441988950276, Blast_Score=72, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17536757, Length=182, Percent_Identity=35.1648351648352, Blast_Score=93, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6324268, Length=217, Percent_Identity=29.0322580645161, Blast_Score=80, Evalue=5e-16,
Organism=Drosophila melanogaster, GI22024126, Length=191, Percent_Identity=34.0314136125654, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR011023 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu [H]

EC number: =2.1.1.178 [H]

Molecular weight: Translated: 35422; Mature: 35291

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSSQILKLARKLELTAEQERDFLDAMQAGNRSRSALVITPKAPEGYRPPLPAKDTPQEW
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHC
GHSSILALPSGEAETKPGSLPDYECGYYYPLDLSSVWETAPLAHLPFRPERCLDMCAAPG
CCCEEEEECCCCCCCCCCCCCCCCCCEEECCCHHHHHCCCCCCCCCCCHHHHHHHHCCCC
GKSILAQTRVSPQEHVSNEVNPKRLGILRHNLLRCGFTNLYTQRLRPDQWAELAPGCFDL
CCHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCEEH
ILADAPCSGQSLLAKGIPNPGCFNSSVTGGNAKRQRGILLAALRCLAPGGFLLYTTCTYA
EEECCCCCCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECC
PEENERNVLYLLKRHPDLHTITVPELEHFRSSLTQEACYRLMPFHGAGAGGFTCLLRKEG
CCCCCCCEEEEEECCCCCEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCC
GHPLPLSLPDELKAWPIHAMNC
CCCCCCCCCHHHCCCCCCCCCC
>Mature Secondary Structure 
SSSQILKLARKLELTAEQERDFLDAMQAGNRSRSALVITPKAPEGYRPPLPAKDTPQEW
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHC
GHSSILALPSGEAETKPGSLPDYECGYYYPLDLSSVWETAPLAHLPFRPERCLDMCAAPG
CCCEEEEECCCCCCCCCCCCCCCCCCEEECCCHHHHHCCCCCCCCCCCHHHHHHHHCCCC
GKSILAQTRVSPQEHVSNEVNPKRLGILRHNLLRCGFTNLYTQRLRPDQWAELAPGCFDL
CCHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCEEH
ILADAPCSGQSLLAKGIPNPGCFNSSVTGGNAKRQRGILLAALRCLAPGGFLLYTTCTYA
EEECCCCCCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECC
PEENERNVLYLLKRHPDLHTITVPELEHFRSSLTQEACYRLMPFHGAGAGGFTCLLRKEG
CCCCCCCEEEEEECCCCCEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCC
GHPLPLSLPDELKAWPIHAMNC
CCCCCCCCCHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA