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The map label for this gene is glkA [H]

Identifier: 187734611

GI number: 187734611

Start: 123193

End: 124152

Strand: Direct

Name: glkA [H]

Synonym: Amuc_0097

Alternate gene names: 187734611

Gene position: 123193-124152 (Clockwise)

Preceding gene: 187734603

Following gene: 187734612

Centisome position: 4.62

GC content: 58.96

Gene sequence:

>960_bases
ATGACAGCTTCAACCATTCCCTCCATCGGCATTGACTTCGGAGGCACCTCCATCAAGATGGGGGTCGTCAAGGGCGCGGA
AGTAATCGCCCACGCTCCTTCAATAGCCACCCAGGAATACGGCAATCCCGATCAACTGATTGAAGCCATAGCCCAGTTCG
TGAATATGCTGCGGCTGAATCACCCGGAAGTGCAGGCCATCGGCATGGGAATGCCGGGGTTCGTCAATTTTTACCAGGGG
ACCGTTTATACGCTTACCAACGTGCCCGGTTGGAACAACGTTCCGGTAAAGGACATGCTCCAGGCTGCCTGCGGACTGCC
CGTATACGTGGAAAACGACGCCAACTGCATGGCTTATGCGGAATGGAAGCTGGGCGCCGGAAAAGGAAAAAGGCATCTGG
TCTGCCTGACCCTCGGCACCGGCGTTGGCAGCGGCCTGATCGTGAACGGAGAACTTCTGCGCGGGGCTACCTGCTCCGCC
GGGGAGCTGGGGCAGACGAGCATCGACTACCGGGGGCGTCTGGGCCATTACGGAAACCGCGGCTCCCTGGAAGATTATGT
AGGCAACCGGGAAATAGCTGCGGATGCACGCACGCTGTATGCCAGCCACGGCATTGACAAGGCCATTGTGGACTGCAACC
CCATCTCTTTGGAACGGGCCGCATTGGCTGGTGATGAAGTAGCCGAGCAAGTATGGCGGGACCTGGCCGTAAAACTCTCC
TGCGCCCTGATGAATTGCTGCTATCTCCTGAACCCGGAAGCCATCATCATCGGCGGGGGCGTGGCCAAGGCCAGAACCCT
GCTTTTCCAGCCCCTTCAGGAAATCATGAAAACCCAGCTCGCCGCCCCTCTGGTGGAATACCTTGAAATCCTTCCCGCCC
AGTTCGGTACGGAGGCGGGCATCCTGGGGGCCGCCCATCTGGCTCTCAACACCCACTTCGGAGAAACATTCCGGGCCTGA

Upstream 100 bases:

>100_bases
CCCCCCCCTTTTGCACCCTCTATTTTCCTGTCGGGATTTTACCGGGAACATACTTCTTTTAACCTTTTCAACGCCCGGTA
AAATTCTATATTGGCGTTAT

Downstream 100 bases:

>100_bases
TCTCGTGTCTTCCTCCCAAATACTCAAACTCGCCCGCAAACTGGAATTGACGGCGGAACAGGAACGGGATTTCCTGGACG
CCATGCAGGCCGGGAACCGT

Product: ROK family protein

Products: NA

Alternate protein names: Glucose kinase [H]

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MTASTIPSIGIDFGGTSIKMGVVKGAEVIAHAPSIATQEYGNPDQLIEAIAQFVNMLRLNHPEVQAIGMGMPGFVNFYQG
TVYTLTNVPGWNNVPVKDMLQAACGLPVYVENDANCMAYAEWKLGAGKGKRHLVCLTLGTGVGSGLIVNGELLRGATCSA
GELGQTSIDYRGRLGHYGNRGSLEDYVGNREIAADARTLYASHGIDKAIVDCNPISLERAALAGDEVAEQVWRDLAVKLS
CALMNCCYLLNPEAIIIGGGVAKARTLLFQPLQEIMKTQLAAPLVEYLEILPAQFGTEAGILGAAHLALNTHFGETFRA

Sequences:

>Translated_319_residues
MTASTIPSIGIDFGGTSIKMGVVKGAEVIAHAPSIATQEYGNPDQLIEAIAQFVNMLRLNHPEVQAIGMGMPGFVNFYQG
TVYTLTNVPGWNNVPVKDMLQAACGLPVYVENDANCMAYAEWKLGAGKGKRHLVCLTLGTGVGSGLIVNGELLRGATCSA
GELGQTSIDYRGRLGHYGNRGSLEDYVGNREIAADARTLYASHGIDKAIVDCNPISLERAALAGDEVAEQVWRDLAVKLS
CALMNCCYLLNPEAIIIGGGVAKARTLLFQPLQEIMKTQLAAPLVEYLEILPAQFGTEAGILGAAHLALNTHFGETFRA
>Mature_318_residues
TASTIPSIGIDFGGTSIKMGVVKGAEVIAHAPSIATQEYGNPDQLIEAIAQFVNMLRLNHPEVQAIGMGMPGFVNFYQGT
VYTLTNVPGWNNVPVKDMLQAACGLPVYVENDANCMAYAEWKLGAGKGKRHLVCLTLGTGVGSGLIVNGELLRGATCSAG
ELGQTSIDYRGRLGHYGNRGSLEDYVGNREIAADARTLYASHGIDKAIVDCNPISLERAALAGDEVAEQVWRDLAVKLSC
ALMNCCYLLNPEAIIIGGGVAKARTLLFQPLQEIMKTQLAAPLVEYLEILPAQFGTEAGILGAAHLALNTHFGETFRA

Specific function: Required for glucose repression of many different genes [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family [H]

Homologues:

Organism=Homo sapiens, GI298566325, Length=274, Percent_Identity=28.8321167883212, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI4885285, Length=274, Percent_Identity=28.8321167883212, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI190014632, Length=275, Percent_Identity=28.7272727272727, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI298566315, Length=274, Percent_Identity=28.8321167883212, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI1787878, Length=265, Percent_Identity=25.6603773584906, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI87081733, Length=310, Percent_Identity=28.7096774193548, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1787363, Length=312, Percent_Identity=28.525641025641, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1786891, Length=259, Percent_Identity=25.0965250965251, Blast_Score=86, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600
- InterPro:   IPR004654 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: =2.7.1.2 [H]

Molecular weight: Translated: 33909; Mature: 33778

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASTIPSIGIDFGGTSIKMGVVKGAEVIAHAPSIATQEYGNPDQLIEAIAQFVNMLRLN
CCCCCCCCCCCCCCCCEEEEEEECCHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHCC
HPEVQAIGMGMPGFVNFYQGTVYTLTNVPGWNNVPVKDMLQAACGLPVYVENDANCMAYA
CCCCEEECCCCCHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHCCCCEEEECCCCEEEEE
EWKLGAGKGKRHLVCLTLGTGVGSGLIVNGELLRGATCSAGELGQTSIDYRGRLGHYGNR
EEEECCCCCCCEEEEEEECCCCCCCEEEECCEECCCCCCCCCCCCCCCCCCCCCCCCCCC
GSLEDYVGNREIAADARTLYASHGIDKAIVDCNPISLERAALAGDEVAEQVWRDLAVKLS
CCHHHHCCCCHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHCHHHHHHHHHHHHHHHHH
CALMNCCYLLNPEAIIIGGGVAKARTLLFQPLQEIMKTQLAAPLVEYLEILPAQFGTEAG
HHHHHHHHEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
ILGAAHLALNTHFGETFRA
HHHHHHHEEECCCCHHHCC
>Mature Secondary Structure 
TASTIPSIGIDFGGTSIKMGVVKGAEVIAHAPSIATQEYGNPDQLIEAIAQFVNMLRLN
CCCCCCCCCCCCCCCEEEEEEECCHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHCC
HPEVQAIGMGMPGFVNFYQGTVYTLTNVPGWNNVPVKDMLQAACGLPVYVENDANCMAYA
CCCCEEECCCCCHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHCCCCEEEECCCCEEEEE
EWKLGAGKGKRHLVCLTLGTGVGSGLIVNGELLRGATCSAGELGQTSIDYRGRLGHYGNR
EEEECCCCCCCEEEEEEECCCCCCCEEEECCEECCCCCCCCCCCCCCCCCCCCCCCCCCC
GSLEDYVGNREIAADARTLYASHGIDKAIVDCNPISLERAALAGDEVAEQVWRDLAVKLS
CCHHHHCCCCHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHCHHHHHHHHHHHHHHHHH
CALMNCCYLLNPEAIIIGGGVAKARTLLFQPLQEIMKTQLAAPLVEYLEILPAQFGTEAG
HHHHHHHHEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
ILGAAHLALNTHFGETFRA
HHHHHHHEEECCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1435260; 12000953 [H]