Click here to switch to the map view.

The map label for this gene is kbl [C]

Identifier: 187734591

GI number: 187734591

Start: 100094

End: 101293

Strand: Direct

Name: kbl [C]

Synonym: Amuc_0077

Alternate gene names: 187734591

Gene position: 100094-101293 (Clockwise)

Preceding gene: 187734590

Following gene: 187734592

Centisome position: 3.76

GC content: 57.92

Gene sequence:

>1200_bases
ATGTATACCCCCGAATTCAAAAACATCCTCACTTCCACCCTGGACGGACTGCGTGCGGAAGGACTTTACAAGGAAGAACG
CTTTATCGCCTCCCAGCAGTACTCCCAGGTGACGCTGAAGGACGGACGCTCCGTCATCAACATGTGCGCCAATAATTATC
TGGGCCTTGCTAATAATCCGGAAGTGATGGAAGCCGCCAAGAAAGCCATCGACCAGTGGGGTTTCGGCATGGCGTCCGTG
CGTTTTATCTGCGGCACGCAGACCCTTCACCGTCAATTGGAAGAACGTCTTACCCAATTTCTCGGCACGGAAGACACGAT
TCTGTTTCCCTCCTGCTTTGACGCCAACGGCGGGCTGTTTGAAGGCCTCCTGACTGCAGACGACGCTATTATTTCCGATT
CCCTGAACCACGCTTCCATTATTGACGGCGTGCGCCTCTGCAAAGCCAAGCGCTTCCGTTATGCCAACAACGACATGGCA
GACCTGGAAGCCAAGCTCCAGGAAGCGGACGCCGCCGGAGCGCGCGTGAAGTTGATTTCCACGGACGGCGTCTTTTCCAT
GGACGGCATCATTGCTCAGCTGGACAAGATTCACGAGCTGGCAGCCAAGTACAATGCCATTGTCCACTTTGACGACTGCC
ACGCCACGGGCTTCCTGGGCGAAAAGGGCCGCGGCACGCATGAATACCGCGGCCTGTTCGGCCATATAGACATCACCACC
GGCACCCTGGGCAAGGCACTGGGAGGCGCTTCCGGCGGCTACGTCTCCGGCCCGAAAGAAGTAGTGGATGTCCTGCGCCA
GAAGGCGCGCCCGTACCTGTTCTCCAACAGCGTGGCCCCGGCTATCGTAGCCGCTTCCATCAAAGTACTGGACCTGTTGG
AACAGTCCACGGAAGCGCGCGACCGCGTAGAAGCCAATACCAAGTATTTCCGTGACGCGATGACGGGAGCCGGATTCACC
ATCGGCGGCAAGGACCACCCCATTTCCCCGGTCATGCTGGGGGATGCCGTCCTGTCCCAGAAATTCTCCGCCCAGCTTCT
GGACGAAGGAGTGTACGCCGTCGGCTTCTTCTATCCCGTCGTTCCCAAGGGACAGGCCCGCATCCGCACGCAGATTTCCG
CAGCCCATACCCGCGAACAGCTGGACAAGGCGATTGAGGCCTTCTGCAAGGTAGGTAAAAACCTGGGCGTTATTTCCTGA

Upstream 100 bases:

>100_bases
CTTTTCCTCCCGCTTCCGTTGTGATAATGATGCGGGAGCAACCGTGTGATCCTTGATCGTTCACTTGCTGACTATTTCAA
TTCATATTCCATTATTCAAC

Downstream 100 bases:

>100_bases
TTCCCGCGCTTTCCATGGCTTCGGACGCATCACCCATGCCCGGCGGCAACCTGCCGACGCTGCCCGTCAAGCGGGTGGTG
CGTCCCACTCCCTCCATTCC

Product: 2-amino-3-ketobutyrate coenzyme A ligase

Products: NA

Alternate protein names: AONS/AKB ligase; 7-keto-8-amino-pelargonic acid synthase; 7-KAP synthase; KAPA synthase; 8-amino-7-ketopelargonate synthase; Alpha-oxoamine synthase; L-alanine--pimeloyl-CoA ligase [H]

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MYTPEFKNILTSTLDGLRAEGLYKEERFIASQQYSQVTLKDGRSVINMCANNYLGLANNPEVMEAAKKAIDQWGFGMASV
RFICGTQTLHRQLEERLTQFLGTEDTILFPSCFDANGGLFEGLLTADDAIISDSLNHASIIDGVRLCKAKRFRYANNDMA
DLEAKLQEADAAGARVKLISTDGVFSMDGIIAQLDKIHELAAKYNAIVHFDDCHATGFLGEKGRGTHEYRGLFGHIDITT
GTLGKALGGASGGYVSGPKEVVDVLRQKARPYLFSNSVAPAIVAASIKVLDLLEQSTEARDRVEANTKYFRDAMTGAGFT
IGGKDHPISPVMLGDAVLSQKFSAQLLDEGVYAVGFFYPVVPKGQARIRTQISAAHTREQLDKAIEAFCKVGKNLGVIS

Sequences:

>Translated_399_residues
MYTPEFKNILTSTLDGLRAEGLYKEERFIASQQYSQVTLKDGRSVINMCANNYLGLANNPEVMEAAKKAIDQWGFGMASV
RFICGTQTLHRQLEERLTQFLGTEDTILFPSCFDANGGLFEGLLTADDAIISDSLNHASIIDGVRLCKAKRFRYANNDMA
DLEAKLQEADAAGARVKLISTDGVFSMDGIIAQLDKIHELAAKYNAIVHFDDCHATGFLGEKGRGTHEYRGLFGHIDITT
GTLGKALGGASGGYVSGPKEVVDVLRQKARPYLFSNSVAPAIVAASIKVLDLLEQSTEARDRVEANTKYFRDAMTGAGFT
IGGKDHPISPVMLGDAVLSQKFSAQLLDEGVYAVGFFYPVVPKGQARIRTQISAAHTREQLDKAIEAFCKVGKNLGVIS
>Mature_399_residues
MYTPEFKNILTSTLDGLRAEGLYKEERFIASQQYSQVTLKDGRSVINMCANNYLGLANNPEVMEAAKKAIDQWGFGMASV
RFICGTQTLHRQLEERLTQFLGTEDTILFPSCFDANGGLFEGLLTADDAIISDSLNHASIIDGVRLCKAKRFRYANNDMA
DLEAKLQEADAAGARVKLISTDGVFSMDGIIAQLDKIHELAAKYNAIVHFDDCHATGFLGEKGRGTHEYRGLFGHIDITT
GTLGKALGGASGGYVSGPKEVVDVLRQKARPYLFSNSVAPAIVAASIKVLDLLEQSTEARDRVEANTKYFRDAMTGAGFT
IGGKDHPISPVMLGDAVLSQKFSAQLLDEGVYAVGFFYPVVPKGQARIRTQISAAHTREQLDKAIEAFCKVGKNLGVIS

Specific function: Catalyzes the decarboxylative condensation of pimeloyl- CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A and/or converts 2-amino-3-ketobutyrate to glycine and acetyl-CoA [H]

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI7657118, Length=396, Percent_Identity=54.2929292929293, Blast_Score=450, Evalue=1e-126,
Organism=Homo sapiens, GI284448556, Length=422, Percent_Identity=50.9478672985782, Blast_Score=440, Evalue=1e-123,
Organism=Homo sapiens, GI119220554, Length=368, Percent_Identity=36.1413043478261, Blast_Score=199, Evalue=4e-51,
Organism=Homo sapiens, GI4758668, Length=368, Percent_Identity=35.5978260869565, Blast_Score=199, Evalue=4e-51,
Organism=Homo sapiens, GI83977442, Length=378, Percent_Identity=30.6878306878307, Blast_Score=196, Evalue=3e-50,
Organism=Homo sapiens, GI83977444, Length=378, Percent_Identity=30.6878306878307, Blast_Score=196, Evalue=3e-50,
Organism=Homo sapiens, GI83977440, Length=378, Percent_Identity=30.6878306878307, Blast_Score=196, Evalue=4e-50,
Organism=Homo sapiens, GI4502025, Length=376, Percent_Identity=30.3191489361702, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI40316939, Length=376, Percent_Identity=30.3191489361702, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI5454084, Length=334, Percent_Identity=28.7425149700599, Blast_Score=118, Evalue=8e-27,
Organism=Escherichia coli, GI1790046, Length=395, Percent_Identity=66.5822784810127, Blast_Score=552, Evalue=1e-158,
Organism=Escherichia coli, GI1786993, Length=352, Percent_Identity=30.6818181818182, Blast_Score=177, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI71994529, Length=400, Percent_Identity=55, Blast_Score=440, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI32566772, Length=407, Percent_Identity=30.958230958231, Blast_Score=193, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI17560914, Length=359, Percent_Identity=32.5905292479109, Blast_Score=175, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI17560912, Length=359, Percent_Identity=32.5905292479109, Blast_Score=175, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI71982625, Length=304, Percent_Identity=25, Blast_Score=101, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI71982617, Length=304, Percent_Identity=25, Blast_Score=101, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6320438, Length=373, Percent_Identity=30.0268096514745, Blast_Score=180, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6320267, Length=370, Percent_Identity=31.3513513513514, Blast_Score=179, Evalue=7e-46,
Organism=Saccharomyces cerevisiae, GI6323954, Length=282, Percent_Identity=29.0780141843972, Blast_Score=114, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24662918, Length=395, Percent_Identity=58.4810126582278, Blast_Score=501, Evalue=1e-142,
Organism=Drosophila melanogaster, GI17137420, Length=369, Percent_Identity=30.8943089430894, Blast_Score=188, Evalue=7e-48,
Organism=Drosophila melanogaster, GI17136286, Length=372, Percent_Identity=32.258064516129, Blast_Score=176, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24653280, Length=302, Percent_Identity=25.1655629139073, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24653276, Length=302, Percent_Identity=25.1655629139073, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24653278, Length=302, Percent_Identity=25.1655629139073, Blast_Score=103, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011282
- InterPro:   IPR001917
- InterPro:   IPR004839
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: =2.3.1.29; =2.3.1.47 [H]

Molecular weight: Translated: 43363; Mature: 43363

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYTPEFKNILTSTLDGLRAEGLYKEERFIASQQYSQVTLKDGRSVINMCANNYLGLANNP
CCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCEEEHHHHHHHHHHHHCCCCCCCCCH
EVMEAAKKAIDQWGFGMASVRFICGTQTLHRQLEERLTQFLGTEDTILFPSCFDANGGLF
HHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCHH
EGLLTADDAIISDSLNHASIIDGVRLCKAKRFRYANNDMADLEAKLQEADAAGARVKLIS
HHHHHHCHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEEE
TDGVFSMDGIIAQLDKIHELAAKYNAIVHFDDCHATGFLGEKGRGTHEYRGLFGHIDITT
CCCCCCHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCHHHCCEEEEEEEEC
GTLGKALGGASGGYVSGPKEVVDVLRQKARPYLFSNSVAPAIVAASIKVLDLLEQSTEAR
CHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHH
DRVEANTKYFRDAMTGAGFTIGGKDHPISPVMLGDAVLSQKFSAQLLDEGVYAVGFFYPV
HHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHCCC
VPKGQARIRTQISAAHTREQLDKAIEAFCKVGKNLGVIS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MYTPEFKNILTSTLDGLRAEGLYKEERFIASQQYSQVTLKDGRSVINMCANNYLGLANNP
CCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCEEEHHHHHHHHHHHHCCCCCCCCCH
EVMEAAKKAIDQWGFGMASVRFICGTQTLHRQLEERLTQFLGTEDTILFPSCFDANGGLF
HHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCHH
EGLLTADDAIISDSLNHASIIDGVRLCKAKRFRYANNDMADLEAKLQEADAAGARVKLIS
HHHHHHCHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEEE
TDGVFSMDGIIAQLDKIHELAAKYNAIVHFDDCHATGFLGEKGRGTHEYRGLFGHIDITT
CCCCCCHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCHHHCCEEEEEEEEC
GTLGKALGGASGGYVSGPKEVVDVLRQKARPYLFSNSVAPAIVAASIKVLDLLEQSTEAR
CHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHH
DRVEANTKYFRDAMTGAGFTIGGKDHPISPVMLGDAVLSQKFSAQLLDEGVYAVGFFYPV
HHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHCCC
VPKGQARIRTQISAAHTREQLDKAIEAFCKVGKNLGVIS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]