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The map label for this gene is leuA [H]

Identifier: 187734579

GI number: 187734579

Start: 87991

End: 89547

Strand: Direct

Name: leuA [H]

Synonym: Amuc_0065

Alternate gene names: 187734579

Gene position: 87991-89547 (Clockwise)

Preceding gene: 187734576

Following gene: 187734580

Centisome position: 3.3

GC content: 58.38

Gene sequence:

>1557_bases
ATGTCCGATCAAATCTACTTTTTTGACACTACCCTGCGCGATGGAGAACAATGCCCCGGAGCATCCATGAACCTACGCGA
AAAGCTGGAAGTGGCGCGTCAAATGGAACGGCTGGGCATGGACGTGATTGAAGCCGGCTTTCCCTGCATCTCCGACGGCG
ACTTTGAAGCCGTGCACACCATCGCCCGCGAAATCAAAAAATGCCGCATCGCTGGTCTGGCCCGCTGCGTAAAGGCGGAC
ATTGAAGCGGCGGCCAAAGCCCTGGAACCCGCCGGGGAACGTGCCCGCATCCACATTTTCCTGGCCACCAGCAAGCTGCA
CATGCAGTTCAAACTGAAAAAAGCGGAATCCGAAATCCTGCGCATGGCCGCGGAAGGCGTATCCTATGCCAAACAATTTG
TCCAGGACGTGGAATTTTCCCCGGAGGACGCTTCCCGCACAGACCTGGACTTCCTGACCAAAGTCGTGGAAACAGTCATC
GACGCCGGCGCCACTACCGTCAACATTCCGGATACCGTGGGCTACACCACACCGGACGAGTTCTACCGCATCATCCGCCA
CCTGAAGGAAAATGTGCCGAACATCGGCAAAGCCGTCATCTCCGTGCATTGCCACAATGACCTTGGCCTGGCCGTAGCAA
ACTCCCTGGCAGCCGTCCGCGCCGGAGCCCGCCAGGTGGAAGGAACCATTAACGGCATCGGCGAACGCGCCGGAAACGTG
GCTCTGGAAGAGGTCATCATGGCCCTGCGCACCCGCGCCGGACAATTCGGAGACGTCACGGACAACATCAACACGAAGGA
AATCGTCCGCACTTCCCGCATCGTGGCCCGCATGAGCGGCATGCAGGTGCAGCGGTCCAAAGCCATCGTGGGGGAAAACG
CTTTCGCCCACTCTTCAGGCATCCACCAGCACGGCATCCTCAACTGCCGGGAGACCTATGAAGTCATGGACCCGCAGGCG
GTCGGCTGGGGAGCCACGGAACTCCCGCTCACCAAGCATTCCGGACGCGCTGCCGTCAAATCCAGGCTGGAACAGCTGGG
CCATGTCCTTACGGAAGAGGAAGTCAACACCGTCTTTGAACGCTTCAAGAAAGTGGGCGACTCCAAAAAATTCGTTTATG
ATGATGATCTTTCCGCCATTGTGGACGACTCCCTGCATGCCTCCACCGGTCTATGGGAACTGGACTTCCTGCAATTCGTG
GCAGGCAGCCACGCGCGGCCCACGGCTACGGTGGGACTGCTCAAGGAAGGCAAGAAATTTGAAGACAGCTCCACGGGCAA
TGGCGCCGTGGACGCCGTTATCAAAGCCATTGAGCGCATCACCAGGCGCAAAGGCACGCTGAAAGGCTACAGCGTCAACG
CCGCTTCCGAAGGGAAGGACGCTCTGGGAGAAGTAACAGTGCATGTGGACTTCGGCCAGCCCAAGCCCATTGTGGGCAAG
GGAGCCTCCACAGATGTCATTGAAGCTTCCGCCCGGGCCTACCTGAACGCGGTCAACCGTTCTATCCGCATGGAAAACAT
GCCCCAGCCCAATAATCTGGATGCAGGAACCATCTAA

Upstream 100 bases:

>100_bases
CCTCTTCCGCCGCCCGGACGCTCTGTGAAAGCATGCAGCGCGTAGGGCTTGCGTTACTGGAGCAAAACATTATCATGCCA
CTGCTTAACATCATTAACCT

Downstream 100 bases:

>100_bases
CACGGGAAGCGCCCCGTTTGCCCGAACGGGGCGCAACTCCGCCCATCAGCCTGCCAGCCTCTGCGGGGCTTGCTTTTCCA
TTGCTCCCATGCCATAAGGT

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 518; Mature: 517

Protein sequence:

>518_residues
MSDQIYFFDTTLRDGEQCPGASMNLREKLEVARQMERLGMDVIEAGFPCISDGDFEAVHTIAREIKKCRIAGLARCVKAD
IEAAAKALEPAGERARIHIFLATSKLHMQFKLKKAESEILRMAAEGVSYAKQFVQDVEFSPEDASRTDLDFLTKVVETVI
DAGATTVNIPDTVGYTTPDEFYRIIRHLKENVPNIGKAVISVHCHNDLGLAVANSLAAVRAGARQVEGTINGIGERAGNV
ALEEVIMALRTRAGQFGDVTDNINTKEIVRTSRIVARMSGMQVQRSKAIVGENAFAHSSGIHQHGILNCRETYEVMDPQA
VGWGATELPLTKHSGRAAVKSRLEQLGHVLTEEEVNTVFERFKKVGDSKKFVYDDDLSAIVDDSLHASTGLWELDFLQFV
AGSHARPTATVGLLKEGKKFEDSSTGNGAVDAVIKAIERITRRKGTLKGYSVNAASEGKDALGEVTVHVDFGQPKPIVGK
GASTDVIEASARAYLNAVNRSIRMENMPQPNNLDAGTI

Sequences:

>Translated_518_residues
MSDQIYFFDTTLRDGEQCPGASMNLREKLEVARQMERLGMDVIEAGFPCISDGDFEAVHTIAREIKKCRIAGLARCVKAD
IEAAAKALEPAGERARIHIFLATSKLHMQFKLKKAESEILRMAAEGVSYAKQFVQDVEFSPEDASRTDLDFLTKVVETVI
DAGATTVNIPDTVGYTTPDEFYRIIRHLKENVPNIGKAVISVHCHNDLGLAVANSLAAVRAGARQVEGTINGIGERAGNV
ALEEVIMALRTRAGQFGDVTDNINTKEIVRTSRIVARMSGMQVQRSKAIVGENAFAHSSGIHQHGILNCRETYEVMDPQA
VGWGATELPLTKHSGRAAVKSRLEQLGHVLTEEEVNTVFERFKKVGDSKKFVYDDDLSAIVDDSLHASTGLWELDFLQFV
AGSHARPTATVGLLKEGKKFEDSSTGNGAVDAVIKAIERITRRKGTLKGYSVNAASEGKDALGEVTVHVDFGQPKPIVGK
GASTDVIEASARAYLNAVNRSIRMENMPQPNNLDAGTI
>Mature_517_residues
SDQIYFFDTTLRDGEQCPGASMNLREKLEVARQMERLGMDVIEAGFPCISDGDFEAVHTIAREIKKCRIAGLARCVKADI
EAAAKALEPAGERARIHIFLATSKLHMQFKLKKAESEILRMAAEGVSYAKQFVQDVEFSPEDASRTDLDFLTKVVETVID
AGATTVNIPDTVGYTTPDEFYRIIRHLKENVPNIGKAVISVHCHNDLGLAVANSLAAVRAGARQVEGTINGIGERAGNVA
LEEVIMALRTRAGQFGDVTDNINTKEIVRTSRIVARMSGMQVQRSKAIVGENAFAHSSGIHQHGILNCRETYEVMDPQAV
GWGATELPLTKHSGRAAVKSRLEQLGHVLTEEEVNTVFERFKKVGDSKKFVYDDDLSAIVDDSLHASTGLWELDFLQFVA
GSHARPTATVGLLKEGKKFEDSSTGNGAVDAVIKAIERITRRKGTLKGYSVNAASEGKDALGEVTVHVDFGQPKPIVGKG
ASTDVIEASARAYLNAVNRSIRMENMPQPNNLDAGTI

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=509, Percent_Identity=48.7229862475442, Blast_Score=459, Evalue=1e-130,
Organism=Saccharomyces cerevisiae, GI6320071, Length=387, Percent_Identity=31.5245478036176, Blast_Score=169, Evalue=7e-43,
Organism=Saccharomyces cerevisiae, GI6320019, Length=369, Percent_Identity=31.1653116531165, Blast_Score=167, Evalue=5e-42,
Organism=Saccharomyces cerevisiae, GI6324225, Length=572, Percent_Identity=23.7762237762238, Blast_Score=134, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6324682, Length=349, Percent_Identity=29.2263610315186, Blast_Score=127, Evalue=4e-30,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 56364; Mature: 56232

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDQIYFFDTTLRDGEQCPGASMNLREKLEVARQMERLGMDVIEAGFPCISDGDFEAVHT
CCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCHHHHHH
IAREIKKCRIAGLARCVKADIEAAAKALEPAGERARIHIFLATSKLHMQFKLKKAESEIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECEEEEEEEHHHHHHHHH
RMAAEGVSYAKQFVQDVEFSPEDASRTDLDFLTKVVETVIDAGATTVNIPDTVGYTTPDE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHH
FYRIIRHLKENVPNIGKAVISVHCHNDLGLAVANSLAAVRAGARQVEGTINGIGERAGNV
HHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALEEVIMALRTRAGQFGDVTDNINTKEIVRTSRIVARMSGMQVQRSKAIVGENAFAHSSG
HHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHCCCC
IHQHGILNCRETYEVMDPQAVGWGATELPLTKHSGRAAVKSRLEQLGHVLTEEEVNTVFE
CCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RFKKVGDSKKFVYDDDLSAIVDDSLHASTGLWELDFLQFVAGSHARPTATVGLLKEGKKF
HHHHCCCCCCEEECCCHHHHHCCCHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCC
EDSSTGNGAVDAVIKAIERITRRKGTLKGYSVNAASEGKDALGEVTVHVDFGQPKPIVGK
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCEEEEEEECCCCCCCCCC
GASTDVIEASARAYLNAVNRSIRMENMPQPNNLDAGTI
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SDQIYFFDTTLRDGEQCPGASMNLREKLEVARQMERLGMDVIEAGFPCISDGDFEAVHT
CCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCHHHHHH
IAREIKKCRIAGLARCVKADIEAAAKALEPAGERARIHIFLATSKLHMQFKLKKAESEIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECEEEEEEEHHHHHHHHH
RMAAEGVSYAKQFVQDVEFSPEDASRTDLDFLTKVVETVIDAGATTVNIPDTVGYTTPDE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHH
FYRIIRHLKENVPNIGKAVISVHCHNDLGLAVANSLAAVRAGARQVEGTINGIGERAGNV
HHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALEEVIMALRTRAGQFGDVTDNINTKEIVRTSRIVARMSGMQVQRSKAIVGENAFAHSSG
HHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHCCCC
IHQHGILNCRETYEVMDPQAVGWGATELPLTKHSGRAAVKSRLEQLGHVLTEEEVNTVFE
CCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RFKKVGDSKKFVYDDDLSAIVDDSLHASTGLWELDFLQFVAGSHARPTATVGLLKEGKKF
HHHHCCCCCCEEECCCHHHHHCCCHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCC
EDSSTGNGAVDAVIKAIERITRRKGTLKGYSVNAASEGKDALGEVTVHVDFGQPKPIVGK
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCEEEEEEECCCCCCCCCC
GASTDVIEASARAYLNAVNRSIRMENMPQPNNLDAGTI
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA