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The map label for this gene is dapF [H]

Identifier: 187734560

GI number: 187734560

Start: 61593

End: 62417

Strand: Direct

Name: dapF [H]

Synonym: Amuc_0044

Alternate gene names: 187734560

Gene position: 61593-62417 (Clockwise)

Preceding gene: 187734559

Following gene: 187734563

Centisome position: 2.31

GC content: 58.3

Gene sequence:

>825_bases
ATGCTGCTTCACTTCTACAAGATGACGGGTGCCGGCAACGACTTTGTCATGGTGGACAATCGTGACCTGAGCCTCTCCTC
CGTGCTGGACAGAGAAACCATAGAAGCCCTGTGTGACCGCCGCTTCGGCATTGGGGCTGACGGGTTGATCGCCGTGGAAC
CTTCCCAGGGCAAGGGGGGAATTGTTCGTATGCGTTATTACAATGCGGACGGCGGAGAAGCGGAAATGTGCGGCAATGGC
GCCCGCTGCTTCGGCAACTTTGCCGCCGCCCTGCTTCATCACGACAAGACAGCCCCCCTTCCTTTTGAAACCATGGCCGG
CGTCGTCACCGCAACCTTTGAAAAAGACGGCAACGTCACCGTAAACCTGACCAATCCCCACTCCATGCAGCTCTTCGTGC
TGAATGCCGATCCGACTGTGGGGGCAGATGTCCATTTCCTGAACACGGGCGTCCCCCATGCCGTTGCGTTTCTGGACAAG
CTGGAGGGCCTGGACATTTCCAGGCTGGGAGCCTATCTGCGTTACCATGAGGCCTTTTCACCCAAAGGCACCAATGCCAA
CTTCGCCAGAATCCTGTCTCCGGGGCACATTGCCATACGTACTTACGAACGCGGCGTAGAGGATGAAACGCTTGCCTGCG
GAACGGGAATGACCGCCTGTGCGCTGCTGCATGCCGTATTGACGGACGCCCCCTCCCCCATTCAGGTGGACGTGGCGGGC
GGAGACACGCTTAAAGTCGGATTCCAACGCACAGGGGACCGTTTCACTGACGTCACGCTGACCGGACCGGCAGACCTTGT
GTTCACCGGAGACATTCAGATCTAA

Upstream 100 bases:

>100_bases
TGACGGCTGTCATTGATGTGGATGCCATGTCATTCATGTAAGCCGCCTTGCCAAAACCGGCCGGGAACATTAGACTTTCG
GCACAACACAACTTACTATT

Downstream 100 bases:

>100_bases
ACAGCTTCGACCTGAAAAGGCGGACTCCTTCCCATCAGGGAAATTATCTGTTCAGCAGGGAACATTCCGCCCGCACATAA
CCGCCGTAATTATTGCAGAA

Product: Diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase [H]

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MLLHFYKMTGAGNDFVMVDNRDLSLSSVLDRETIEALCDRRFGIGADGLIAVEPSQGKGGIVRMRYYNADGGEAEMCGNG
ARCFGNFAAALLHHDKTAPLPFETMAGVVTATFEKDGNVTVNLTNPHSMQLFVLNADPTVGADVHFLNTGVPHAVAFLDK
LEGLDISRLGAYLRYHEAFSPKGTNANFARILSPGHIAIRTYERGVEDETLACGTGMTACALLHAVLTDAPSPIQVDVAG
GDTLKVGFQRTGDRFTDVTLTGPADLVFTGDIQI

Sequences:

>Translated_274_residues
MLLHFYKMTGAGNDFVMVDNRDLSLSSVLDRETIEALCDRRFGIGADGLIAVEPSQGKGGIVRMRYYNADGGEAEMCGNG
ARCFGNFAAALLHHDKTAPLPFETMAGVVTATFEKDGNVTVNLTNPHSMQLFVLNADPTVGADVHFLNTGVPHAVAFLDK
LEGLDISRLGAYLRYHEAFSPKGTNANFARILSPGHIAIRTYERGVEDETLACGTGMTACALLHAVLTDAPSPIQVDVAG
GDTLKVGFQRTGDRFTDVTLTGPADLVFTGDIQI
>Mature_274_residues
MLLHFYKMTGAGNDFVMVDNRDLSLSSVLDRETIEALCDRRFGIGADGLIAVEPSQGKGGIVRMRYYNADGGEAEMCGNG
ARCFGNFAAALLHHDKTAPLPFETMAGVVTATFEKDGNVTVNLTNPHSMQLFVLNADPTVGADVHFLNTGVPHAVAFLDK
LEGLDISRLGAYLRYHEAFSPKGTNANFARILSPGHIAIRTYERGVEDETLACGTGMTACALLHAVLTDAPSPIQVDVAG
GDTLKVGFQRTGDRFTDVTLTGPADLVFTGDIQI

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family [H]

Homologues:

Organism=Escherichia coli, GI87082334, Length=285, Percent_Identity=31.2280701754386, Blast_Score=114, Evalue=5e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001653
- InterPro:   IPR018510 [H]

Pfam domain/function: PF01678 DAP_epimerase [H]

EC number: =5.1.1.7 [H]

Molecular weight: Translated: 29296; Mature: 29296

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLHFYKMTGAGNDFVMVDNRDLSLSSVLDRETIEALCDRRFGIGADGLIAVEPSQGKGG
CEEEEEEECCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCC
IVRMRYYNADGGEAEMCGNGARCFGNFAAALLHHDKTAPLPFETMAGVVTATFEKDGNVT
EEEEEEECCCCCCCEECCCCHHHHHHHHHHHHHCCCCCCCCHHHHCCEEEEEEECCCCEE
VNLTNPHSMQLFVLNADPTVGADVHFLNTGVPHAVAFLDKLEGLDISRLGAYLRYHEAFS
EEEECCCEEEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCC
PKGTNANFARILSPGHIAIRTYERGVEDETLACGTGMTACALLHAVLTDAPSPIQVDVAG
CCCCCCCEEEEECCCEEEEEEECCCCCCCEEECCCCHHHHHHHHHHHHCCCCCEEEEECC
GDTLKVGFQRTGDRFTDVTLTGPADLVFTGDIQI
CCEEEEEEECCCCEEEEEEEECCCCEEEEECEEC
>Mature Secondary Structure
MLLHFYKMTGAGNDFVMVDNRDLSLSSVLDRETIEALCDRRFGIGADGLIAVEPSQGKGG
CEEEEEEECCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCC
IVRMRYYNADGGEAEMCGNGARCFGNFAAALLHHDKTAPLPFETMAGVVTATFEKDGNVT
EEEEEEECCCCCCCEECCCCHHHHHHHHHHHHHCCCCCCCCHHHHCCEEEEEEECCCCEE
VNLTNPHSMQLFVLNADPTVGADVHFLNTGVPHAVAFLDKLEGLDISRLGAYLRYHEAFS
EEEECCCEEEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCC
PKGTNANFARILSPGHIAIRTYERGVEDETLACGTGMTACALLHAVLTDAPSPIQVDVAG
CCCCCCCEEEEECCCEEEEEEECCCCCCCEEECCCCHHHHHHHHHHHHCCCCCEEEEECC
GDTLKVGFQRTGDRFTDVTLTGPADLVFTGDIQI
CCEEEEEEECCCCEEEEEEEECCCCEEEEECEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA