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The map label for this gene is priA [H]

Identifier: 187734559

GI number: 187734559

Start: 59317

End: 61533

Strand: Direct

Name: priA [H]

Synonym: Amuc_0043

Alternate gene names: 187734559

Gene position: 59317-61533 (Clockwise)

Preceding gene: 187734558

Following gene: 187734560

Centisome position: 2.23

GC content: 60.44

Gene sequence:

>2217_bases
ATGCAGGCGGCGCGCATTCTGGTTGACGGACAAAGCGATCTGGTACTGGATTACGGTATTCCTCCGGAAGCGGGAGACGT
CAAGCCGGGCTGCCGCGTACAGGTGCCCCTGCGCAACAGAACGGCTACCGGAACCGTGCTTACGCTGTCAGAACCGGCCC
CGGCCTGGAAGGACAGGCTCAAGCCCATTCTGAAACTGATCGATCCGGAGCCCCTGATTTCCCCGGTGATGATGAATCTG
GCCTCATGGGCGGCGGACTATTACTCCGTGGCTCTGGATCAGATGATCCGGTGTCTGCTCCCGGAAACCGTTCGTCAGGA
AAATACGGCGGAAAAAATGCGCAAAATGGTGTATCTGGAAAAAACTCCGGCACGGGAGGAACTGGACGCCCTGTACCGCA
AAGCACCCCGGCAGGCCCAAATGCTGGATTATTTCTCATCCGCGAAACAACAGTCCGCCCCCCTGGCGGCATTCGGCGCC
GGAGCCCTGAACGTTGCCCGCAGCCTGGAAGCCAAAGGCTTCATCTCCCTGAAAGAAGAGGCCGTGCACCGGGACCCCAG
CACCGGGGAGCAATTCGTCCCCACCCAGCCCATGAAGCTGAACAGCCAGCAGCAGAAAGCGCTGGAAGAAATCACGGCCA
TGTGCGCGGCAGAGCGCAAAAAACCGGTCCTGCTGCAAGGAGTTACCGGTTCCGGCAAGACGGAGGTTTACTTACAGGCC
GTTTCCCAAATAGTAAAATCCGGAAAATCCGCCCTGATCATGGTGCCGGAAATTTCCCTGACGCCCCAGACGGTTCAGCG
TTTCAAATCCCGTTTCGCAGAACTGCCTTCCTCCGTGGCAGTGCTGCACAGCCTCCTCTCGGACGGGGAGCGCTTTGACG
AATGGCATGCCATCCGTTCCGGAAAAGCCCGCATCGTCATCGGTCCCCGTTCCGCCGTCTTCGCCCCCCTCCAAAATCTG
GGCCTGGTGATTGTGGATGAAGAACACGACGCCTCTTACAAACAGGAAAGCTCCCCCCGCTACCACGGGCGGGATCTGGC
CGTGCTGCGCGCCCATCTGGAAAATTGCGCCGTGCTTCTTGGCTCTGCCACTCCCTCCCTGGAAAGCATCCATAACGCCC
TGATTGGAAAATATTCCCTGGTAAAACTGACGGAAAGGGCGGACGGCCAGCAGCTCCCGCTCATTCGCATCCTGGACATG
AAAACGGAAGGAAGGAACAAATCCGGTCCCAACGTTATCTCCGAACGCCTCAGGATGTCCATTGACCGGCGTCTCGACAA
GGGGGAACAGGTCATCCTGCTGCTCAACAGGCGCGGATTCGCACGCTCCATCCAATGCCCGGACTGCGGCCACGTAGTCA
CGTGCCTGCACTGCTCCCTGCCCCTGACCTACCACCGTACGGAAGACCGCCTCATGTGCCACCTGTGCGGATTCAAAGCC
CTCCCGCCCCGTTCCTGCCCGGAATGCCGGTCCGCCAACATCCTGCTCCAGGGGTACGGAACCCAGAAAGTGGAGGAACT
CCTGCGCCGCACCTTCCCTGCGGCGCGCATCACGCGCGTGGATGCGGACGTGGCCCGGCGGAAAAACGCCGTCCGGACCA
TTCTGAACCAGTTTCGCGCCCATAAGATAGATATTCTCCTGGGCACCCAAATGATCGCCAAAGGTTTGGATTTTCCCAAC
GTGACGCTGGTAGGCGTACTGAACGCGGACCTGGGGCTCCACATCCCGGATCCCCGTGCAGGGGAACGCACTTTCCAGCT
CCTGACGCAGGTAGCGGGCCGCGCCGGCCGCGGCGATCTTTCCGGAGAAGTCATTATCCAGACTTTTACGCCCCAGTCCC
CCTCCCTGCAATACGCCCGGCACCATGACACGGACGGCTTTGCAGCCCAGGAACTGGAAATGCGGCGTACTTTCGACCTC
CCCCCCTTCACCCATATCGCCGTCTTGACCATACGTTCCCAGCATGAAAGCATGGCGGAATTCGCCACGCAAACCCTCGC
GGCCCGCCTGCGCGGCATGCTCCCCCCTCCCGCCACGATGACCGACCCCATGCCTGCCCCCATCCCCCGTGCGCACGGAC
AGTTCAGATTCCAGATTACGGTCAAGGGGCCATCCGCCCGCATCCTCTCCCGCACTCTCCGGAAACTGGTGCAGGAAGCC
GGCCTGGGGGAAGACTTGACGGCTGTCATTGATGTGGATGCCATGTCATTCATGTAA

Upstream 100 bases:

>100_bases
GGATGGATACGGCTACAATTACATTGAAGTTCTGAAGGACATGAGCGGCGTCTCCCGTTTCCCCTTCGGCTATCGTTAAT
GTTATCCTCCTTTTCACTCC

Downstream 100 bases:

>100_bases
GCCGCCTTGCCAAAACCGGCCGGGAACATTAGACTTTCGGCACAACACAACTTACTATTATGCTGCTTCACTTCTACAAG
ATGACGGGTGCCGGCAACGA

Product: primosomal protein N'

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 738; Mature: 738

Protein sequence:

>738_residues
MQAARILVDGQSDLVLDYGIPPEAGDVKPGCRVQVPLRNRTATGTVLTLSEPAPAWKDRLKPILKLIDPEPLISPVMMNL
ASWAADYYSVALDQMIRCLLPETVRQENTAEKMRKMVYLEKTPAREELDALYRKAPRQAQMLDYFSSAKQQSAPLAAFGA
GALNVARSLEAKGFISLKEEAVHRDPSTGEQFVPTQPMKLNSQQQKALEEITAMCAAERKKPVLLQGVTGSGKTEVYLQA
VSQIVKSGKSALIMVPEISLTPQTVQRFKSRFAELPSSVAVLHSLLSDGERFDEWHAIRSGKARIVIGPRSAVFAPLQNL
GLVIVDEEHDASYKQESSPRYHGRDLAVLRAHLENCAVLLGSATPSLESIHNALIGKYSLVKLTERADGQQLPLIRILDM
KTEGRNKSGPNVISERLRMSIDRRLDKGEQVILLLNRRGFARSIQCPDCGHVVTCLHCSLPLTYHRTEDRLMCHLCGFKA
LPPRSCPECRSANILLQGYGTQKVEELLRRTFPAARITRVDADVARRKNAVRTILNQFRAHKIDILLGTQMIAKGLDFPN
VTLVGVLNADLGLHIPDPRAGERTFQLLTQVAGRAGRGDLSGEVIIQTFTPQSPSLQYARHHDTDGFAAQELEMRRTFDL
PPFTHIAVLTIRSQHESMAEFATQTLAARLRGMLPPPATMTDPMPAPIPRAHGQFRFQITVKGPSARILSRTLRKLVQEA
GLGEDLTAVIDVDAMSFM

Sequences:

>Translated_738_residues
MQAARILVDGQSDLVLDYGIPPEAGDVKPGCRVQVPLRNRTATGTVLTLSEPAPAWKDRLKPILKLIDPEPLISPVMMNL
ASWAADYYSVALDQMIRCLLPETVRQENTAEKMRKMVYLEKTPAREELDALYRKAPRQAQMLDYFSSAKQQSAPLAAFGA
GALNVARSLEAKGFISLKEEAVHRDPSTGEQFVPTQPMKLNSQQQKALEEITAMCAAERKKPVLLQGVTGSGKTEVYLQA
VSQIVKSGKSALIMVPEISLTPQTVQRFKSRFAELPSSVAVLHSLLSDGERFDEWHAIRSGKARIVIGPRSAVFAPLQNL
GLVIVDEEHDASYKQESSPRYHGRDLAVLRAHLENCAVLLGSATPSLESIHNALIGKYSLVKLTERADGQQLPLIRILDM
KTEGRNKSGPNVISERLRMSIDRRLDKGEQVILLLNRRGFARSIQCPDCGHVVTCLHCSLPLTYHRTEDRLMCHLCGFKA
LPPRSCPECRSANILLQGYGTQKVEELLRRTFPAARITRVDADVARRKNAVRTILNQFRAHKIDILLGTQMIAKGLDFPN
VTLVGVLNADLGLHIPDPRAGERTFQLLTQVAGRAGRGDLSGEVIIQTFTPQSPSLQYARHHDTDGFAAQELEMRRTFDL
PPFTHIAVLTIRSQHESMAEFATQTLAARLRGMLPPPATMTDPMPAPIPRAHGQFRFQITVKGPSARILSRTLRKLVQEA
GLGEDLTAVIDVDAMSFM
>Mature_738_residues
MQAARILVDGQSDLVLDYGIPPEAGDVKPGCRVQVPLRNRTATGTVLTLSEPAPAWKDRLKPILKLIDPEPLISPVMMNL
ASWAADYYSVALDQMIRCLLPETVRQENTAEKMRKMVYLEKTPAREELDALYRKAPRQAQMLDYFSSAKQQSAPLAAFGA
GALNVARSLEAKGFISLKEEAVHRDPSTGEQFVPTQPMKLNSQQQKALEEITAMCAAERKKPVLLQGVTGSGKTEVYLQA
VSQIVKSGKSALIMVPEISLTPQTVQRFKSRFAELPSSVAVLHSLLSDGERFDEWHAIRSGKARIVIGPRSAVFAPLQNL
GLVIVDEEHDASYKQESSPRYHGRDLAVLRAHLENCAVLLGSATPSLESIHNALIGKYSLVKLTERADGQQLPLIRILDM
KTEGRNKSGPNVISERLRMSIDRRLDKGEQVILLLNRRGFARSIQCPDCGHVVTCLHCSLPLTYHRTEDRLMCHLCGFKA
LPPRSCPECRSANILLQGYGTQKVEELLRRTFPAARITRVDADVARRKNAVRTILNQFRAHKIDILLGTQMIAKGLDFPN
VTLVGVLNADLGLHIPDPRAGERTFQLLTQVAGRAGRGDLSGEVIIQTFTPQSPSLQYARHHDTDGFAAQELEMRRTFDL
PPFTHIAVLTIRSQHESMAEFATQTLAARLRGMLPPPATMTDPMPAPIPRAHGQFRFQITVKGPSARILSRTLRKLVQEA
GLGEDLTAVIDVDAMSFM

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=732, Percent_Identity=37.9781420765027, Blast_Score=446, Evalue=1e-126,
Organism=Escherichia coli, GI1787357, Length=479, Percent_Identity=23.1732776617954, Blast_Score=74, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 81648; Mature: 81648

Theoretical pI: Translated: 9.40; Mature: 9.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAARILVDGQSDLVLDYGIPPEAGDVKPGCRVQVPLRNRTATGTVLTLSEPAPAWKDRL
CCCCEEEEECCCCEEEEECCCCCCCCCCCCCEEEEECCCCCCCCEEEEECCCCCHHHHHH
KPILKLIDPEPLISPVMMNLASWAADYYSVALDQMIRCLLPETVRQENTAEKMRKMVYLE
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHC
KTPAREELDALYRKAPRQAQMLDYFSSAKQQSAPLAAFGAGALNVARSLEAKGFISLKEE
CCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHCCCCCEEEHHH
AVHRDPSTGEQFVPTQPMKLNSQQQKALEEITAMCAAERKKPVLLQGVTGSGKTEVYLQA
HHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHH
VSQIVKSGKSALIMVPEISLTPQTVQRFKSRFAELPSSVAVLHSLLSDGERFDEWHAIRS
HHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHC
GKARIVIGPRSAVFAPLQNLGLVIVDEEHDASYKQESSPRYHGRDLAVLRAHLENCAVLL
CCEEEEECCCHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHEEEE
GSATPSLESIHNALIGKYSLVKLTERADGQQLPLIRILDMKTEGRNKSGPNVISERLRMS
CCCCCCHHHHHHHHHCCHHEEEEHHCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHH
IDRRLDKGEQVILLLNRRGFARSIQCPDCGHVVTCLHCSLPLTYHRTEDRLMCHLCGFKA
HHHHCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCCEEECCCHHHHHHHHCCCC
LPPRSCPECRSANILLQGYGTQKVEELLRRTFPAARITRVDADVARRKNAVRTILNQFRA
CCCCCCCCCCCCCEEEECCCHHHHHHHHHHHCCHHHEEECCHHHHHHHHHHHHHHHHHHH
HKIDILLGTQMIAKGLDFPNVTLVGVLNADLGLHIPDPRAGERTFQLLTQVAGRAGRGDL
HEEEEEECHHHHHCCCCCCCEEEEEEEECCCCEECCCCCCCHHHHHHHHHHHCCCCCCCC
SGEVIIQTFTPQSPSLQYARHHDTDGFAAQELEMRRTFDLPPFTHIAVLTIRSQHESMAE
CCCEEEEEECCCCCCCCHHHCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHH
FATQTLAARLRGMLPPPATMTDPMPAPIPRAHGQFRFQITVKGPSARILSRTLRKLVQEA
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHC
GLGEDLTAVIDVDAMSFM
CCCCCCEEEEECHHHHCC
>Mature Secondary Structure
MQAARILVDGQSDLVLDYGIPPEAGDVKPGCRVQVPLRNRTATGTVLTLSEPAPAWKDRL
CCCCEEEEECCCCEEEEECCCCCCCCCCCCCEEEEECCCCCCCCEEEEECCCCCHHHHHH
KPILKLIDPEPLISPVMMNLASWAADYYSVALDQMIRCLLPETVRQENTAEKMRKMVYLE
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHC
KTPAREELDALYRKAPRQAQMLDYFSSAKQQSAPLAAFGAGALNVARSLEAKGFISLKEE
CCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHCCCCCEEEHHH
AVHRDPSTGEQFVPTQPMKLNSQQQKALEEITAMCAAERKKPVLLQGVTGSGKTEVYLQA
HHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHH
VSQIVKSGKSALIMVPEISLTPQTVQRFKSRFAELPSSVAVLHSLLSDGERFDEWHAIRS
HHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHC
GKARIVIGPRSAVFAPLQNLGLVIVDEEHDASYKQESSPRYHGRDLAVLRAHLENCAVLL
CCEEEEECCCHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHEEEE
GSATPSLESIHNALIGKYSLVKLTERADGQQLPLIRILDMKTEGRNKSGPNVISERLRMS
CCCCCCHHHHHHHHHCCHHEEEEHHCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHH
IDRRLDKGEQVILLLNRRGFARSIQCPDCGHVVTCLHCSLPLTYHRTEDRLMCHLCGFKA
HHHHCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCCEEECCCHHHHHHHHCCCC
LPPRSCPECRSANILLQGYGTQKVEELLRRTFPAARITRVDADVARRKNAVRTILNQFRA
CCCCCCCCCCCCCEEEECCCHHHHHHHHHHHCCHHHEEECCHHHHHHHHHHHHHHHHHHH
HKIDILLGTQMIAKGLDFPNVTLVGVLNADLGLHIPDPRAGERTFQLLTQVAGRAGRGDL
HEEEEEECHHHHHCCCCCCCEEEEEEEECCCCEECCCCCCCHHHHHHHHHHHCCCCCCCC
SGEVIIQTFTPQSPSLQYARHHDTDGFAAQELEMRRTFDLPPFTHIAVLTIRSQHESMAE
CCCEEEEEECCCCCCCCHHHCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHH
FATQTLAARLRGMLPPPATMTDPMPAPIPRAHGQFRFQITVKGPSARILSRTLRKLVQEA
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHC
GLGEDLTAVIDVDAMSFM
CCCCCCEEEEECHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377; 9086272 [H]